CHEMISTRY OF MATERIALS
·
2024
·
IF 7.2
Tiansheng Gan,Wenhui Shang,Stephan Handschuh-Wang,Yaokang Zhang,Xuechang Zhou
Galvanic replacement reaction (GRR) holds immense potential for engineering metal nanostructures. This reaction allows for the formation of bimetallic hollow nanostructures in a single step, in which precise control of the reaction is necessary to customize the size, composition, and morphology. Whereas achieving trimetallic or multimetallic systems through sequential GRR is highly desirable, their synthesis remains challenging because of the high complexity of multistep GRR processes and the fact that they require precise control of the addition sequence and amount of metal precursors. Here, we introduce a simplified sequential GRR approach, namely, “living” galvanic replacement reaction (LGRR). The LGRR utilizes a liquid metal (LM) nanoreactor consisting of a chemically active LM core and a polydopamine (PDA) shell. The LM core undergoes stepwise reactions with various dissolved metal ions, leading to the nucleation and growth of reduced metal nanocrystals on the PDA shell within minutes at room temperature. Importantly, the LM core remains active (“living”), and the LGRR continues uninterrupted by both the deposited metals and the addition sequence of the metal precursors. We demonstrate that the LGRR of LM nanoreactors facilitates the stepwise growth of different metal nanocrystals when different metal precursors are added sequentially, resulting in the formation of diverse multimetallic nanostructures that possess a phase-segregated multimetallic shell surrounding an LM core. Furthermore, the LGRR of the LM nanoreactors also enables the rapid synthesis of nanoalloys within 5 min by simultaneously reducing different precursors.
研究领域:
材料科学
·
纳米技术
·
化学
·
冶金学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Jing Peng,Simin Liu,Ziyue Qiu,Luyin Zhang,Ziyan Dong,Qianying Zhou,Ting Huang,Yanfei Zhang,Jingyu Shi,Jin-Xiang Chen,Zong Dai,Jun Chen
miRNA is an effective disease diagnostic biomarker. Developing efficient miRNA in living cells analysis methods, holds significant importance for disease diagnosis and understanding disease progression. Strategies combining DNA nanostructures and enzyme-free amplification have been developed for highly sensitive and rapid imaging of miRNA. However, due to nonspecific amplification of enzyme-free amplification inherent in the probe assembly process and during transfection, these strategies suffer from significant background signals and reduced sensitivity, limiting their further application. To overcome these limitations, we have developed a DNA tetrahedral-based and photocontrolled intramolecular CHA strategy (TCHA-P) to achieve spatiotemporal, highly sensitive, and accurate imaging of miRNA in living cells. In this strategy, the DNA tetrahedron not only serves as a transfection vector but also enhances the local concentration of reactant probes, boosting the reaction kinetics and sensitivity of CHA. Additionally, the light-controlled group (PC-linker) can trigger CHA at selected times and locations, enhancing the spatiotemporal precision of miRNA imaging and ensuring high sensitivity and accuracy by eliminating nonspecific amplification during the assembly and transfection processes. Using miR-10b as a proof-of-concept model, the proposed strategy demonstrated higher signal-to-noise ratio compared to traditional CHA, with a detection limit of 28 pM. Living cell imaging experiments showed that the strategy could effectively differentiate the expression of miR-10b between normal breast cells and breast cancer cells, providing high imaging contrast. The strategy holds promise for broadening the path of nucleic acid amplification-based imaging and is expected to become an effective tool in nucleic acid-based disease diagnosis, treatment, and drug development.
研究领域:
分子生物学
·
纳米生物学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2017
·
IF 3.133
Kui Liang,Hailin Yang,Wenxun Guo,Jianlong Du,Lingyun Tian,Xiaofeng Wen
Silicon has been regarded as a promising alternative anode material for next-generation lithium ion batteries (LIBs) due to its high theoretical specific capacity of 3590 mAh g −1 (Li 3.75 Si formed at room temperature). In this work, nanoscale silicon has been synthesized via a simple and effective metathesis reaction in which SiCl 4 is the silicon source and Zn is the reductant and solvent, at a mild temperature of 500 °C. The preparation process is very simple, facile and low-cost. The as-prepared silicon particles are spherical with a diameter of 150–200 nm, and the delithiation capacity of the nanoscale silicon can reach 1747.7 mAh g −1 at a current density of 0.2 A g −1 .
研究领域:
材料科学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Shaoqiong Feng,Hanjun Chen,Ziao Hu,Tingting Wu,Zhihong Liu
MicroRNA (miRNA) is a promising biomarker for the diagnosis, monitoring, and prognostic evaluation of diseases, especially cancer. The existing miRNA detection methods usually need external instruments for quantitative signal output, limiting their practical applications in point-of-care (POC) settings. Here, we propose a distance-based biosensor through a responsive hydrogel, in combination with a CRISPR/Cas12a system and target-triggered strand displacement amplification (SDA) reaction for visual quantitative and sensitive measurement of miRNA. The target miRNA is first converted into plenty of double-stranded DNA (dsDNA) via target-triggered SDA reaction. Then, the dsDNA products trigger the collateral cleavage activity of CRISPR/Cas12a, leading to the release of trypsin from magnetic beads (MBs). The released trypsin can hydrolyze gelatin, and hence the permeability of gelatin-treated filter paper is increased, resulting in a visible distance signal on a cotton thread. Using this system, the concentration of the target miRNA can be quantified visually without any assistance of instruments, and a detection limit of 6.28 pM is obtained. In addition, the target miRNA in human serum samples and cell lysates can also be detected accurately. Owing to the characteristics of simplicity, sensitivity, specificity, and portability, the proposed biosensor provides a new tool for miRNA detection and holds great promise in POC applications.
研究领域:
生物技术
·
分子诊断
·
医学检验
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Xiaojuan Liu,Ningning Xu,Panpan Gai,Feng Li
Since melamine is a strong hazard to human health, the development of new methods for highly sensitive detection of melamine is highly desirable. Herein, a novel fluorescent biosensing strategy was designed for sensitive and selective melamine assay based on the recognition ability of abasic (AP) site in triplex towards melamine and signal amplification by Mg 2+ -dependent DNAzyme. In this strategy, the melamine-induced formation of triplex DNA was employed to trigger the strand displacement reaction (SDR). The SDR process converted the specific target recognition into the release and activation of Mg 2+ -dependent DNAzyme, which could catalyze the cleavage of fluorophore/quencher labeled DNA substrate (FQ), resulting in a significantly increased fluorescent signal. Under the optimal conditions, the fluorescent signal has a linear relationship with the logarithm of the melamine concentration in a wide range of 0.005–50 μM. The detection limit was estimated to be 0.9 nM (0.1ppb), which is sufficiently sensitive for practical application. Furthermore, this strategy exhibits high selectivity against other potential interfering substances, and the practical application of this strategy for milk samples reveals that the proposed strategy works well for melamine assay in real samples. Therefore, this strategy presents a new method for the sensitive melamine assay and holds great promise for sensing applications in the environment and the food safety field.
研究领域:
生物化学
·
食品安全
·
分析化学
✓ 已验证
DOI
高影响因子
CRYSTENGCOMM
·
2023
·Vol. 25
·期 28
·4039-4047
·
IF 3.1
Guoliang Feng,Jintao Gao,Xi Lan,Yu Li,Zhancheng Guo
Vanadium (V) is often transferred into vanadium slag from the smelting of vanadium titano-magnetite ore, however, basic data for the crystallization behavior and crystal structure of V-spinel are lacking, which limits the efficient recovery of V resources from vanadium slag. In this study, the crystallization behavior of V-spinel in vanadium slag was investigated, and in situ separation of high-purity V-spinel crystals from vanadium slag was conducted via super-gravity at 1673 K. It was found that TiO2 content of 12 wt% in vanadium slag was the most conducive to the crystallization of V-spinel. On this basis, the crystal structures of various V-spinel crystals were characterized, by which the displacement mechanism of V and Ti in V-spinel was verified. Namely, V3+ in the octahedron sites was gradually replaced by Ti4+ with the increase of Ti content, while an equal amount of Fe3+ in the tetrahedron sites was replaced by Fe2+. This study provides some necessary theoretical basis for the efficient recovery of V resources in vanadium slag.
研究领域:
冶金学
·
材料科学
✓ 已验证
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2023
·
IF 2.9
Zongkang Guo,Xueting Fan,Xiaoli Wang,Zhemin Zhou,Yuting Zhang,Nandi Zhou
Mec A, as a representative gene mediating resistance to β‐lactam antibiotics in methicillin-resistant Staphylococcus aureus (MRSA), allows a new genetic analysis for the detection of MRSA. Here, a sensitive, prompt, and visual colorimetry is reported to detect the Mec A gene based on toehold‐mediated strand displacement (TMSD) and the enrichment effect of graphene oxide (GO). The Mec A triggers to generate the profuse amount of signal units of single-stranded DNA (SG) composed of a long single‐stranded base tail and a base head: the tail can be adsorbed and enriched on the surface of GO; the head can form a G quadruplex structure to exert catalytic function towards 2,2′‐azino‐bis (3‐ethylbenzthiazoline‐6‐sulphonic acid). Therefore, through the enrichment effect of GO, the signal units SG reflects different degrees of signal amplification on different substrates (such as aqueous solution or filter membrane). This strategy demonstrates a broad linear working range from 100 pM to 1.5 nM (solution) and 1 pM to 1 nM (filter membrane), with a low detection limit of 39.53 pM (solution) and 333 fM (filter membrane). Analytical performance in real samples suggests that this developed colorimetry is endowed with immense potential for clinical detection applications.
研究领域:
生物化学
·
医学诊断
·
纳米材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Mingyan Jia,Yixuan Pan,Jianbo Zhou,Mei Zhang
In this work, we developed an optical colorimetric sensor array for the discrimination of Chinese teas. The sensor array was carefully designed based on tea polyphenol induced indicators displacement assay (IDA), using phenylboronic acids with different substituents as the receptors to polyphenols. The accurate identification for polyphenols with different species or concentrations proved the potential of the sensor array. The sensor array successfully distinguished tea samples within different categories, grades and origins, coupling with PLS-DA. This work offered an efficient and rapid method to distinguish teas and tea-related products. Besides, the assay is supposed to be suitable for the identification of other polyphenol-related natural products.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
SENSORS
·
2020
·Vol. 20
·期 5
·1331
·
IF 3.275
Yue Wang,Huan Feng,Haibo Li,Xinyi Yang,Hongmin Jia,Wenjun Kang,Qingtao Meng,Zhiqiang Zhang,Run Zhang
Quick and effective detection of biothiols in biological fluids has gained increasing attention due to its vital biological functions. In this paper, a novel reversible fluorescence chemosensor (L-Cu2+) based on a benzocoumarin-Cu2+ensemble has been developed for the detection of biothiols (Cys, Hcy and GSH) in human urine. The chemosensing ensemble (L-Cu2+) contains a 2:1 stoichiometry structure between fluorescent ligandLand paramagnetic Cu2+.Lwas found to exclusively bond with Cu2+ions accompanied with a dramatic fluorescence quenching maximum at 443 nm and an increase of an absorbance band centered at 378 nm. Then, the in situ generated fluorescence sluggish ensemble,L-Cu2+, was successfully used as a chemosensor for the detection of biothiols with a fluorescence “OFF-ON” response modality. Upon the addition of biothiols, the decomplexation ofL-Cu2+led to the liberation of the fluorescent ligand,L, resulting in the recovery of fluorescence and absorbance spectra. Studies revealed thatL-Cu2+possesses simple synthesis, excellent stability, high sensitivity, reliability at a broad pH range and desired renewability (at least 5 times). The practical application ofL-Cu2+was then demonstrated by the detection of biothiols in human urine sample.Keywords:chemosensor;biothiols;displacement;detection;urine samples
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Jinzhi Liu,Mengyan Wang,Chi Zhang,Gaiping Li,Qi Shen,Lina Zou
Herein, a “signal-on” electrochemical aptasensor based on dual-output toehold mediated strand displacement reaction (TMSDR) was developed to detect cytokeratin fragment antigen 21–1 (CYFRA 21–1). First, the methylene blue (MB) labeled signal probe (SP) was combined with a long template strand (TS) to form the three-strands duplex, which was immobilized on magnetic beads. The sequence of TS was innovatively designed with two toehold zones. Thus, with the presence of target DNA and Fuel strand (F), double SP and target were released through TMSDR. Besides, the released target could participate in the next cycle, achieving the further amplification. The released SP was selected by magnetic separation, then added to the gold electrode (GE) to hybridize with the hairpin DNA. Ultimately, the target can be detected by recording the current response of the MB. Under optimized conditions, the response peak current was linear with the concentration of CYFRA 21–1 in range from 10 pM to 1 μM with detection limit of 8.079 pM. The proposed signal amplification strategy was enzyme-free in homogenous solution and the using of magnetic beads improved the recognition efficiency and simplified the experimental steps, which endowed the sensor with super-sensitivity.
研究领域:
生物化学
·
医学检测
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 2
·1-7
·
IF 5.705
Ge Jia,Bai Dong-Mei,-Geng Xin,Hu Ya-Lei,Cai Qi-Yong,Xing Ke,Zhang Lin,Li Zhao-Hui
The authors describe a fluorometric method for the quantitation of nucleic acids by combining (a) cycled strand displacement amplification, (b) the unique features of the DNA probe SYBR Green, and (c) polydopamine nanotubes. SYBR Green undergoes strong fluorescence enhancement upon intercalation into double-stranded DNA (dsDNA). The polydopamine nanotubes selectively adsorb single-stranded DNA (ssDNA) and molecular beacons. In the absence of target DNA, the molecular beacon, primer and SYBR Green are adsorbed on the surface of polydopamine nanotubes. This results in quenching of the fluorescence of SYBR Green, typically measured at excitation/emission wavelengths of 488/518 nm. Upon addition of analyte (target DNA) and polymerase, the stem of the molecular beacon is opened so that it can bind to the primer. This triggers target strand displacement polymerization, during which dsDNA is synthesized. The hybridized target is then displaced due to the strand displacement activity of the polymerase. The displaced target hybridizes with another molecular beacon. This triggers the next round of polymerization. Consequently, a large amount of dsDNA is formed which is detected by addition of SYBR Green. Thus, sensitive and selective fluorometric detection is realized. The fluorescent sensing strategy shows very good analytical performances towards DNA detection, such as a wide linear range from 0.05 to 25 nM with a low limit of detection of 20 pM.
Schematic of a fluorometric strategy for highly sensitive and selective determination of nucleic acids by combining strand displacement amplification and the unique features of SYBR Green I (SG) and polydopamine nanotubes.
研究领域:
分析化学
·
分子生物学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Xianfeng Wang,Chengxiang Chu,Jiayi Lv,Yuanyuan Jia,Libo Lin,Mei Yang,Suyi Zhang,Danqun Huo,Changjun Hou
High-performance analysis of heavy metal ions is great importance in both environment and food safety. In this work, a facile and reliable colorimetric sensor was presented for simultaneous detection of Cu 2+ and Cr 3+ based on indicator-displacement assay (IDA). As a typical silicate nanomaterials , ZnSiO 3 hollow nanosphere (ZSHS) exhibited an outstanding ion exchange capacity. Zincon was incorporated with the ZSHS to form a zincon/ZSHS hybrid ionophore with a blue color. Upon the addition of Cr 3+ , IDA reaction and selective ion exchange occurred with the color change of zincon/ZSHS ionophore from blue to yellow. With such a design, colorimetric measurement of Cr 3+ was realized. The linear concentration for Cr 3+ detection ranged from 0.5 μM to 75 μM with the LOD of 83.2 nM. Furthermore, we also screened different kinds of complexing agents that may respond with zincon/ZSHS ionophore and various metal ions. It was found that tartaric acid (TA) showed the chelation capability of Zn 2+ -TA is stronger than that of Zn 2+ -zincon. Thus zincon/ZSHS/TA presented a yellow color due to the chelation reaction of Zn 2+ -TA, releasing the zincon as a free state. After addition of Cu 2+ , a stronger chelation reaction of Cu 2+ -zincon occurred. This process involved in the color change from yellow to blue and realized colorimetric measurement of Cu 2+ . The detection limit of Cu 2+ was calculated to be 43.7 nM with linear range from 0.1 to 20 μM. In addition, the zincon/ZSHS nanoprobe was successfully applied for simultaneous measurement of Cu 2+ and Cr 3+ in sorghum and river water, indicating that the zincon/ZSHS nanoprobe provided a promising sensing platform in environment and food safety.
研究领域:
环境科学
·
食品安全
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）