FORENSIC SCIENCE INTERNATIONAL
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2014
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IF 2.115
Jinjie Wang,Qian Lai,Hongyang Pan,Daming Sun,Chunfeng Yu,Wei Zhang,Jian Chen,Liqin Ma,Ling Li,Ren Zhou
Objective To explore the value of CK13 (Rab) and CK10/13(Mab) as objective and specific biomarkers combined with Alcian-Phloxine-Martius yellow (APM) staining for the diagnosis of amniotic fluid embolism (AFE) and amniotic fluid aspiration (AFA). Methods A retrospective study of forensic autopsy cases of 148 neonatal deaths and 19 maternal deaths in the perinatal stage was conducted at the Institute of Pathology and Forensic Medicine, Zhejiang University. Medical records were reviewed and monoclonal antibody for CK13 (Rab) and CK10/13 (Mab) as immunostaining of amniotic fluid squamous cells, APM staining, and Hematoxylin and Eosin (HE) staining were used to diagnose the AFE and AFA. Descriptive statistics of the patient population were analyzed using SPSS 20.0 software. Results Immunoreactivity of CK13 and CK10/13 specifically identified squamous cells of all the AFE and AFA cases. The amniotic fluid squamous cells were stained positive in a deep brown color with the monoclonal antibody to CK 13 and CK10/13 whereas the endothelial cells and alveolar epithelial cells were negative. There was no CK13 or CK10/13 expression in the non-AFE and non-AFA cases. With APM staining keratinized squamous cells were pink and mucus was blue-green in marked contrast with the surrounding tissue, which improved the detection rates of both keratinized squamous cells and mucus. Conclusions CK13 (Rab) and CK10/13 (Mab) are valuable and reliable biomarkers of amniotic fluid squamous cells. APM reveals enriched mucus and keratinized squamous cells of amniotic fluid. Immunohistochemical detection of CK13 and CK10/13 combined with APM staining can improve the accuracy and reduce the difficulty in the diagnosis of AFE and AFA.
研究领域:
病理学
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法医学
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妇产科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
HUMAN & EXPERIMENTAL TOXICOLOGY
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2014
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IF 1.407
MS Acar,ZB Bulut,A Ateş,B Nami,N Koçak,B Yıldız
Titanium dioxide (TiO2) nanoparticles (NPs) are commonly used materials present in many consumables for which most people are exposed to. The biological hazards of the NPs on human health have been demonstrated previously. In this study, we aimed to assess the cytotoxicity potency of TiO2 NPs on the primary human amniotic fluid cells. The cells derived from amniotic fluid were treated with different dosages of TiO2 NPs for some periods. Cell adhesion status was assessed using a light microscopic observation. Cell proliferation and cell death rates were determined using trypan blue staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Also, mitotic index was determined using fluorescence in situ hybridization with chromosome 8 centromer-specific DNA probe. Disrupted cell adhesion, decreased proliferation, and increased mortality rates were detected in the cells that were treated with TiO2 NPs depending on the dosage (p < 0.001). Also, reduced mitotic index was determined in the cells depending on the time and TiO2 dosage when compared with the controls (p < 0.0001). These results showed that TiO2 NPs have high cytotoxicity for amniotic fluid-derived cells. Therefore, different products containing TiO2 NPs should be used with care, especially for pregnant women.
研究领域:
毒理学
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Zhen Li,Xingtong Liu,Linna Li,Yandi Hui,Lili Liu,Fujun Yao,Xiaofeng Kang,Yanli Guo
Sickle cell disease (SCD) is an autosomal recessive genetic disease that is caused by a single nucleotide mutation from adenine to thymine in the β-bead protein gene (HBB gene). Currently, there is no completely effective cure for SCD, and management is primarily to prevent. Therefore, timely detection of the genotype is crucial for subsequent diagnosis. In this study, we developed a rapid and straightforward method utilizing the high spatiotemporal resolution of the α-hemolysin (α-HL) nanopore to detect wild-type (HBBW) and mutant (HBBM) HBB gene. This approach uses the anthracycline drug doxorubicin (DOX) as a probe and leverages the distinct characteristic signals generated from its interactions with HBBW and HBBM. When DOX interacts with HBBW, it forms a double-stranded structure of the DNA-DOX complexes, whereas it induces the formation of hairpin structures when interacting with HBBM. These two structures exhibit significant differences in dwell time and amplitude, enabling clear and efficient identification. By mixing different ratios of HBBW and HBBM, we were able to accurately identify genotypes based on the peak area occupied by the characteristic HBBM signals. This method has been successfully applied for detection of HBBM in simulated artificial amniotic fluid (AAF), demonstrating its practicality and reliability. Our approach enables early diagnosis of SCD at the single-molecule level, providing a new avenue for rapid detection of single-nucleotide mutations in gene sequences using DNA–drug interactions.
研究领域:
生物物理学
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遗传学
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分子诊断学
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纳米技术
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药物基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EXPERIMENTAL ANIMALS
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2016
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IF 1.247
Yunyun Tian,Li Tao,Siriguleng Zhao,Dapeng Tai,Dongjun Liu,Pengxia Liu
Mesenchymal stem cells (MSCs) are one of the most promising cell populations for tissue engineering and regenerative medicine. Of utmost importance to MSC research is identification of MSC sources that are easily obtainable and stable. Several studies have shown that MSCs can be isolated from amniotic fluid. The sheep is one of the main types of farm animal, and it has many biophysical and biochemical similarities to humans. Here, we obtained MSCs from ovine amniotic fluid and determined the expansion capacity, surface and intracellular marker expression, karyotype, and multilineage differentiation ability of these ovine amniotic fluid mesenchymal stem cells (oAF-MSCs). Moreover, expression levels of differentiation markers were measured using reverse transcription-qPCR (RT-qPCR). Our phenotypic analysis shows that the isolated oAF-MSCs are indeed MSCs.
研究领域:
干细胞生物学
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再生医学
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组织工程
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兽医学
✓ 已验证
DOI
PubMed
高影响因子
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