FORENSIC SCIENCE INTERNATIONAL
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2014
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IF 2.115
Jinjie Wang,Qian Lai,Hongyang Pan,Daming Sun,Chunfeng Yu,Wei Zhang,Jian Chen,Liqin Ma,Ling Li,Ren Zhou
Objective To explore the value of CK13 (Rab) and CK10/13(Mab) as objective and specific biomarkers combined with Alcian-Phloxine-Martius yellow (APM) staining for the diagnosis of amniotic fluid embolism (AFE) and amniotic fluid aspiration (AFA). Methods A retrospective study of forensic autopsy cases of 148 neonatal deaths and 19 maternal deaths in the perinatal stage was conducted at the Institute of Pathology and Forensic Medicine, Zhejiang University. Medical records were reviewed and monoclonal antibody for CK13 (Rab) and CK10/13 (Mab) as immunostaining of amniotic fluid squamous cells, APM staining, and Hematoxylin and Eosin (HE) staining were used to diagnose the AFE and AFA. Descriptive statistics of the patient population were analyzed using SPSS 20.0 software. Results Immunoreactivity of CK13 and CK10/13 specifically identified squamous cells of all the AFE and AFA cases. The amniotic fluid squamous cells were stained positive in a deep brown color with the monoclonal antibody to CK 13 and CK10/13 whereas the endothelial cells and alveolar epithelial cells were negative. There was no CK13 or CK10/13 expression in the non-AFE and non-AFA cases. With APM staining keratinized squamous cells were pink and mucus was blue-green in marked contrast with the surrounding tissue, which improved the detection rates of both keratinized squamous cells and mucus. Conclusions CK13 (Rab) and CK10/13 (Mab) are valuable and reliable biomarkers of amniotic fluid squamous cells. APM reveals enriched mucus and keratinized squamous cells of amniotic fluid. Immunohistochemical detection of CK13 and CK10/13 combined with APM staining can improve the accuracy and reduce the difficulty in the diagnosis of AFE and AFA.
研究领域:
病理学
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法医学
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妇产科学
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DOI
PubMed
高影响因子
Journal of Environmental Sciences
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2020
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IF 4.302
Qiangying Zhang,Ming Que,Wei Li,Shuang Gao,Xin Tan,Duo Bu
Gangba sheep are known for having typical sensory characteristics attributed to free range conditions and grazing on wild plants. The genuine Gangba mutton was selected as the experimental group, and the commercial Tibetan mutton was selected as the control group, the nutritive composition of basic chemical components, amino acids and fatty acids in mutton were investigated to correlate its unique meat quality and eating satisfaction. The results showed that fatty acids were significantly higher ( P <0.05) in Gangba mutton than in commercial mutton, and the higher content of flavoring amino acids (glutamic acid and aspartame) were primarily responsible for the taste attributes umami of meat juices. Moreover, the trace elements analysis in mutton and grazing factors (forage, water source and soil) were conducted, to explain the source of essential trace elements in mutton. The concentrations of essential trace elements show that the Gangba mutton was a valuable source for highly available Cu and Zn in human nutrition, and well managed with few detected of toxicity metal. The concentrations of essential trace elements in mutton are closely related to the trace elements in environmental grazing factors. In conclusion, the congenital grazing conditions (a highly mineralized water resource, natural forages and clean soils) were shown to contribute to the unique meat characteristics of Gangba sheep.
研究领域:
动物科学
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食品科学
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营养学
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DOI
PubMed
高影响因子
Separations
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2024
·Vol. 11
·期 9
·276
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IF 2.5
Kaidierya Abudureheman,Qinglin Wang,Hao Zhang,Xingchu Gong
The Analytical Quality by Design (AQbD) concept was adopted to establish a quantitative analysis of multi-components with a single marker (QAMS) method for industrial lanolin alcohol, targeting cholesterol, lanosterol, and 24,25-dihydrolanosterol. The potential critical method parameters (CMPs) were identified as column temperature, flow rate, and gradient. Definitive screening design and statistical modeling were employed to optimize the gradient conditions of the mobile phase, column temperature, and flow rate. The Method Operable Design Region (MODR) was determined using a risk-based quantification approach. The robustness was assessed using a Plackett–Burman experimental design, followed by methodological validation. Optimal analytical conditions were as follows: acetonitrile (B)—water (A) mobile phase system; flow rate of 1.58 mL/min; detection wavelength of 205 nm; injection volume of 10 µL; and column temperature of 37 °C. A gradient elution program was implemented as follows: 0–19.0 min, 90.5% B; 19.0–25.0 min, 90.5–100% B; and 25.0–55.0 min, 100% B. Cholesterol served as an internal standard for quantifying lanosterol and 24,25-dihydrolanosterol, with relative correction factors of 0.4227 and 0.8228, respectively. This analytical method utilized only the cholesterol reference substance as an internal standard to quantify the content of cholesterol, lanosterol, and 24,25-dihydrolanosterol in industrial lanolin alcohol. It reduced the testing costs and enhanced efficiency, making it potentially suitable for widespread adoption in lanolin alcohol processing industries.
研究领域:
分析化学
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生物医学
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DOI
高影响因子
Frontiers in Nutrition
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2023
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IF 5
Li Jing,Tang Chaohua,Yang Youyou,Hu Ying,Zhao Qingyu,Ma Qing,Yue Xiangpeng,Li Fadi,Zhang Junmin
Sheep breed has a major influence on characteristics of meat quality and intramuscular fat (IMF), however, studies into the relationship between sheep breed and meat quality traits rarely consider the large variation in IMF within breed. In this study, groups of 176 Hu and 76 Tan male sheep were established, weaned at 56 days old, with similar weights, and representative samples were selected based on the distribution of IMF in each population, to investigate variations in meat quality, IMF and volatile compound profiles between breeds. Significant differences were observed in drip loss, shear force, cooking loss, and color coordinates between Hu and Tan sheep (p < 0.01). The IMF content and the predominate unsaturated fatty acids, oleic and cis, cis-linoleic acids, were similar. Eighteen out of 53 volatile compounds were identified as important odor contributors. Of these 18 odor-active volatile compounds, no significant concentration differences were detected between breeds. In another 35 volatile compounds, γ-nonalactone was lower in Tan sheep relative to Hu sheep (p < 0.05). In summary, Tan sheep exhibited lower drip loss, higher shear force values, and redder color, had less saturated fatty acids, and contained less γ-nonalactone against Hu sheep. These findings improve understanding of aroma differences between Hu and Tan sheep meat.
研究领域:
动物科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
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2024
·Vol. 14
·期 5
·240
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IF 4.9
Dongpeng Luo,Limin Xu,Lifeng Jia,Lianglun Cheng,Ping Tang,Jinyun Zhou
Cashmere and wool are both natural animal fibers used in the textile industry, but cashmere is of superior quality, is rarer, and more precious. It is therefore important to distinguish the two fibers accurately and effectively. However, challenges due to their similar appearance, morphology, and physical and chemical properties remain. Herein, a terahertz electromagnetic inductive transparency (EIT) metasurface biosensor is introduced for qualitative and quantitative identification of cashmere and wool. The periodic unit structure of the metasurface consists of four rotationally symmetric resonators and two cross−arranged metal secants to form toroidal dipoles and electric dipoles, respectively, so that its effective sensing area can be greatly improved by 1075% compared to the traditional dipole mode, and the sensitivity will be up to 342 GHz/RIU. The amplitude and frequency shift changes of the terahertz transmission spectra caused by the different refractive indices of cashmere/wool can achieve highly sensitive label−free qualitative and quantitative identification of both. The experimental results show that the terahertz metasurface biosensor can work at a concentration of 0.02 mg/mL. It provides a new way to achieve high sensitivity, precision, and trace detection of cashmere/wool, and would be a valuable application for the cashmere industry.
研究领域:
材料科学
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纺织工程
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传感技术
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
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IF 5.2
Fenghou Yuan,Wanyue Liu,Binghui Song,Jing Wang,Fan Li,Tian Liu
The sheep lice eggshells and felting are both important problem in wool industry. Deep eutectic solvents (DES) are a class of eco-friendly and low-cost solvents that are used in various fields including the anti-felting of wool but not in the eggshell removal. In this study, the simultaneous removal of wool scales and lice eggshells using [ChCl:Urea] was achieved by optimizing the formula and condition. Satisfactory eggshell removal (80.0%) and anti-felting rates (22.7%) had little effect on the mechanical strength of the wool (12.1%). The mechanism of the beneficial effects was studied using scanning electron microscopy and Fourier-transform infrared spectroscopy. The loose structure of protein cement is conducive to the penetration of DESs to achieve complete disintegration and dissolution. In contrast, the dense wool structure prevents DESs from penetrating the interior and only removes the surface scales. In addition, dissolution of eggshells by DES also helps in its removal. To our knowledge, this is the first report of DES-mediated one-step eggshell removal and anti-felting of wool. Through economic and environmental effects analyses, DES-based technique exhibits a more promising potential for industrial applications. This work not only has significant application prospects in the wool industry, but also the application scenarios of DESs.
研究领域:
纺织科学
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环境科学
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生物学
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DOI
高影响因子
Microbial Cell Factories
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2023
·Vol. 22
·期 1
·1-11
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IF 6.4
Lin Yu,Chen Wen-wen,Ding Bo,Guo Man,Liang Meng,Pang Hao,Wei Yu-tuo,Huang Ri-bo,Du Li-qin
Background Icaritin is an aglycone of flavonoid glycosides from Herba Epimedii. It has good performance in the treatment of hepatocellular carcinoma in clinical trials. However, the natural icaritin content of Herba Epimedii is very low. At present, the icaritin is mainly prepared from flavonoid glycosides by α-L-rhamnosidases and β-glucosidases in two-step catalysis process. However, one-pot icaritin production required reported enzymes to be immobilized or bifunctional enzymes to hydrolyze substrate with long reaction time, which caused complicated operations and high costs. To improve the production efficiency and reduce costs, we explored α-L-rhamnosidase SPRHA2 and β-glucosidase PBGL to directly hydrolyze icariin to icaritin in one-pot, and developed the whole-cell catalytic method for efficient icaritin production.Results The SPRHA2 and PBGL were expressed in Escherichia coli, respectively. One-pot production of icaritin was achieved by co-catalysis of SPRHA2 and PBGL. Moreover, whole-cell catalysis was developed for icariin hydrolysis. The mixture of SPRHA2 cells and PBGL cells transformed 200 g/L icariin into 103.69 g/L icaritin (yield 95.23%) in 4 h in whole-cell catalysis under the optimized reaction conditions. In order to further increase the production efficiency and simplify operations, we also constructed recombinant E. coli strains that co-expressed SPRHA2 and PBGL. Crude icariin extracts were also efficiently hydrolyzed by the whole-cell catalytic system.Conclusion sCompared to previous reports on icaritin production, in this study, whole-cell catalysis showed higher production efficiency of icaritin. This study provides promising approach for industrial production of icaritin in the future.
研究领域:
生物技术
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药学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Bopei Wei,Shuaihao Wang,Jiaxin Wang,Man Zhou,Ping Wang,Yuanyuan Yu,Qiang Wang
Nowadays, the Chlorination-Hercosett process dominates the production of machine-washable wool fabric. However, absorbable organic halogens (AOX) generated during the treatment are highly detrimental to human health and the environment. Herein, we developed an innovative pad-steam-pad-cold batch enzymatic treatment with the merit of being eco-friendly, water-saving, and cost-effective to replace traditional high pollution chlorination technique. With the assistance of sodium sulfite or L-cysteine, a broad-temperature-range proteinase (BTRP) can efficiently hydrolyze wool scales during the steaming and cold batch processes. The results indicate that BTRP treatment endows the wool fabric with excellent dimensional stability, acceptable strength loss, and improved wearability properties such as drapability, and air and moisture permeability. This non-chlorination treatment achieves a felting shrinkage of 4.33 % ± 0.28 %, and a tensile strength loss of 12.36 % ± 1.46 %. Furthermore, colored wool samples treated with BTRP were shown to maintain their original color performance, including K/S value and various color fastness properties. Pilot plant tests indicates that this technique remarkably decreased the cost of chemical materials and water usage during enzymatic treatment. As an ideal alternative substitute for the Chlorination-Hercosett treatment, the “pad-steam-pad-cold batch” approach based on BTRP treatment can be utilized for commercial production of pristine and colored machine-washable wool garments.
研究领域:
纺织科学
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生物技术
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环境科学
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产品关联
DOI
PubMed
高影响因子
Phytochemistry Letters
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2023
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IF 1.7
Yu-Chen Chen,Qiong Zheng,Hui Lu,Yi-Xin Zhuang,Ting-Ting Gong,Lie-Feng Ma,Zha-Jun Zhan
A new flavonoid glycoside bearing an unusual furan ring, epimedokoreanin F ( 1 ), was isolated from Epimedium brevicornu , along with 17 known flavonoids ( 2−18 ). The structures of these flavonoids were identified based on interpretation of spectroscopic data and comparison with literature data. Biologically, compounds 1−4 exhibited significant neuroprotective effects on the model of RSL3-induced ferroptosis in HT22 cells. Among them, compound 1 displayed the best activity with EC 50 values of 0.95 μ M, and can be used as lead compound for the development of neuroprotective agent to treat neurological diseases .
研究领域:
药理学
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神经科学
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生物化学
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植物化学
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产品关联
DOI
高影响因子
Metabolites
·
2023
·Vol. 13
·期 2
·294
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IF 4.1
Linlin Yang,Fei Zhang,Yueci Yan,Xupeng Gu,Shengwei Zhou,Xiuhong Su,Baoyu Ji,Hua Zhong,Chengming Dong
The harvesting period is a critical period for the accumulation of flavonoids in the leaves of the important medicinal plantEpimedium sagittatum. In this study, we conducted an experiment onE. sagittatumleaves sprayed with mineral elements with the aim of improving the quality of the herbal leafage during the harvesting period. We elucidated the changes in flavonoids (icariin, epimedin A, epimedin B, and epimedin C) inE. sagittatumleaves. The sum of main flavonoids content reached a maximum (11.74%) at 20 days after the high-concentration Fe2+(2500 mg·L−1) treatment. We analyzed the FT-IR spectra characteristics ofE. sagittatumleaf samples using the FT-IR technique, and constructed an OPLS-DA model and identified characteristic peaks to achieve differentiated identification ofE. sagittatum. Further, widely untargeted metabolomic analysis identified different classes of metabolites. As the most important characteristic flavonoids, the relative contents of icariin, icaritin, icariside I, and icariside II were found to be up-regulated by high-Fe2+treatment. Our experimental results demonstrate that high-concentration Fe2+treatment is an effective measure to increase the flavonoids content inE. sagittatumleaves during the harvesting period, which can provide a scientific basis for the improvement ofE. sagittatumleaf cultivation agronomic measures.Keywords:Epimedium sagittatum;flavonoids;icariin;FT-IR;metabolomic
研究领域:
生药学
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植物生理学
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分析化学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Veterinary Science
·
2025
·
IF 2.9
Jie Luo,Yiping Wang,Wenya Zheng,Chaolong Xu,Jianhua Chen,Ben Liu,Xiaoyue Wang,Manxin Fang
Introduction Mastitis outbreaks in dairy goats at a farm in western Jiangxi Province, have recently imposed significant economic losses on farmers. This study aimed to identify the causative pathogen and implement targeted control measures for early disease eradication. Methods Pathological autopsy was performed on diseased goats submitted for examination. Pathological mammary gland tissues and milk cistern fluid were collected aseptically for bacterial isolation and purification. The isolated pathogen was characterized through colony morphology observation, Gram staining, biochemical tests, and 16S rDNA gene sequencing. Pathogenicity was assessed using an animal challenge test in mice. In vitro antibacterial susceptibility testing was conducted using 30 Antibiotics (e.g., cefradine, cephalexin, cefuroxime), 18 Chinese herbal medicines (CHMs; e.g., Rhus chinensis (Galla Chinensis), Coptis chinensis (Chinese Goldthread), Punica granatum (Granati Pericarpium)), and 15 phytogenic extracts (e.g., Berberine, Gallic acid, Tannic acid). Results A Gram-positive bacterium was isolated from the diseased tissues. Biochemical profiling and 16S rDNA sequencing identified the isolate as Staphylococcus caprae . The animal challenge test confirmed the isolate’s strong pathogenicity in mice. Susceptibility testing indicated that the isolate was susceptible to Antibiotics such as cefradine, cephalexin, cefuroxime, and ciprofloxacin, while showing the poorest susceptibility to penicillin among the tested antibiotics. Among the CHMs and phytogenic extracts, Rhus chinensis (Galla Chinensis), Coptis chinensis (Chinese Goldthread), Punica granatum (Granati Pericarpium), Berberine, Gallic acid and Tannic acid demonstrated superior antibacterial efficacy. Discussion This study provides multiple therapeutic and preventive options (CHMs, Antibiotics, phytogenic extracts) for mastitis control on this farm. It also offers valuable insights into the identification and treatment of mastitis in dairy goats, contributing to safeguarding goat health and ensuring dairy product safety.
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DOI
PubMed
高影响因子
Chemistry Africa-A Journal of the Tunisian Chemical Society
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2025
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IF 2.2
Chen Wang,Zhang Fen,Xu Jianghe,Lai Feifan
Purpose To immobilize snailase on SBA-15 to enhance the efficient conversion of Epimedium wushanense extracts for the preparation of rhamnosyl icariside II. Methods The snailase was covalently coupled to amino and aldehyde modified SBA-15, and the immobilization conditions were optimized. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), N 2 adsorption-desorption analysis, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to characterize the physicochemical properties of immobilized snailase. The optimal enzymatic hydrolysis conditions, enzymatic kinetic parameters, storage stability and reusability of the immobilized snailase were investigated. Results The optimal immobilization conditions were pH 5.0, temperature 30℃, immobilization time 3 h and snailase concentration 10 mg⋅mL − 1 . The immobilized snailase showed a retained activity of 235.8 µmol⋅(h⋅g) −1 and a high enzyme loading capacity of 249.6 mg⋅g − 1 support. The optimum hydrolysis conditions for immobilized snailase were pH 5.5, temperature 50℃, substrate concentration 3.0 mg⋅mL − 1 , and reaction time 6 h. The V max and K m of the immobilized snailase were 3.92 µg⋅min − 1 and 1.18 mmol⋅L − 1 , respectively. Conclusion The immobilized snailase has a high enzyme loading capacity, strong conversion capability, better storage stability and good reusability, making it suitable for the efficient conversion of Epimedium wushanense extracts to produce rhamnosyl icariside II.
研究领域:
酶学
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生物催化
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植物化学
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纳米技术
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生物技术
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）