ACTA PHARMACOLOGICA SINICA
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2026
·
IF 10.4
Rao Fang,Tang Ming-xin,Yang Hang,Wang Tian-hai,Zhou Chun-yi,Xiao Cheng
Chronic neuropathic pain is sustained by nerve injury-induced central sensitization and is characterized by both sensory and affective manifestations. However, its underlying neural circuit mechanisms remain to be fully elucidated. Here, we revealed that CaMKII-expressing neurons in the parasubthalamic nucleus (PSTN) were recruited and exhibited hyperactivity in a neuropathic pain mouse model, established via spared nerve injury of the sciatic nerve (SNI). Optogenetic and chemogenetic inhibition of PSTN CaMKII neurons alleviated hyperalgesia and induced conditioned place preference (CPP) in SNI mice, without altering pain thresholds in sham mice. Mechanistically, we demonstrated that PSTN neurons received monosynaptic glutamatergic inputs from tachykinin precursor 1 (Tac1)-expressing neurons in the lateral parabrachial nucleus (LPB), and were excited by glutamate and substance P (SP) co-released from this projection. Inhibition of the LPB–PSTN projection recapitulated the analgesic and CPP-inducing effects of direct inhibition of PSTN neurons in SNI mice. Notably, these therapeutic effects were mimicked by local administration of a neurokinin receptor 1 (NK1R) antagonist, but were reversed by intra-PSTN injection of SP. Together, this study not only establishes the involvement of the LPB–PSTN circuit and local NK1R in the pathophysiology of neuropathic pain but also demonstrates their promise as therapeutic targets.
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高影响因子
Coatings
·
2023
·Vol. 13
·期 4
·733
·
IF 3.4
Meizhu Qin,Xinping Hu,Jingyan Guo
Polystyrene (PS) is a widely used building insulation material with good mechanical strength and strong temperature adaptability. However, PS itself is highly flammable and displays poor flame retardancy. At present, building fires caused by organic external wall thermal insulation materials prepared from PS represent a new fire hazard. In this study, the addition of an intumescent flame retardant (IFR) to reduce the flammability of PS was achieved. Using melamine (MEL), acrylonitrile-styrene-acrylate (ASA), and phytic acid (PA) as raw materials, a new type of flame retardant (MAP) was prepared by an electrostatic self-assembly method and was introduced to modify PS. Its effect on the flammability of PS composites was also investigated. The flammability of the PS composites was characterized using the limiting oxygen index (LOI) and vertical combustion. The effect of MAP on the morphology of the carbon layer formed from polymer decomposition was studied using scanning electron microscopy (SEM). By adding MAP to a PS/20%N-IFR flame-retardant composite, the flame-retardant property was significantly improved, the limiting oxygen index reached 37, and the vertical combustion reached a V-0 level. The fire performance index (FPI) of the PS/20%N-IFR composite reached 0.0054, which was significantly higher than that of the control PS (0.037) as determined by the cone calorimetry test. The SEM results showed that the introduction of MAP can increase the density of the carbon layer after combustion. The heat release rate for combustion was reduced. In addition, the mechanical properties of the PS/20%N-IFR composites were compared with those with no flame retardant. The tensile strength of the PS/20%N-IFR composite was 26.1 MPa and the elongation of the PS/20%N-IFR composite remained at 2.2%. The PS/20%N-IFR composite displayed better flame retardancy than the untreated material and good mechanical properties. The presence of MAP prevented the heat and oxygen transfer and interrupted the releasing of flammable products, thus protecting the PS from burning. This flame-retardant material may find broad applications in building insulation materials.Keywords:intumescent flame retardant;polystyrene;insulation materials
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Microbiology Spectrum
·
2022
·
IF 9.043
Chenghua Song,Ruichao Wen,Jiaxuan Zhou,Xiaoyan Zeng,Zi Kou,Yufeng Li,Feng Yun,Rongqian Wu
Due to the changes in pathogenic species and the absence of research on topical skin antibiotics, the therapy of skin and soft tissue infections (SSTIs) is facing more and more severe challenges. It is particularly urgent to look for alternative therapies without induction of drug resistance. UV C (UVC) light within the range of 200 to 280 nm is one of the most common techniques used to kill and/or inactivate pathogenic microorganisms. However, the traditional most commonly used wavelength of 254 nm irradiated from a low-pressure mercury lamp is hazardous to human health, being both carcinogenic and damaging to eye tissues, which limits its applications in vivo. This research aimed to investigate the antimicrobial properties and influence of 275-nm UVC light from a light-emitting diode (UVC-LED light) on wound healing time. Five bacteria, three fungi, and scalded-mouse models combined with SSTIs were used to evaluate the antimicrobial effect in vitro and in vivo. 275-nm UVC-LED light inactivated both bacteria and fungi with a very short irradiation time in vitro and induced neither DNA damage nor epidermal lesions in the mice’s skin. Furthermore, in mouse models of SSTIs induced by either methicillin-resistant Staphylococcus aureus (MRSA) or Candida albicans, the 275-nm UVC-LED light showed significant antimicrobial effects and shortened the wound healing time compared with that in the no-irradiation group. UVC-LED light at 275 nm has the potential to be a new form of physical therapy for SSTIs.IMPORTANCE As a common clinical problem, the therapy of SSTIs is facing growing challenges due to an increase in the number of drug-resistant bacteria and fungi. UV C (UVC) light sterilization has been widely used in all aspects of daily life, but there are very few reports about in vivo therapy using UVC light. It is well known that prolonged exposure to UVC light increases the possibility of skin cancer. In addition, it is also very harmful for eyes. UV irradiation with 254-nm UVC light can cause corneal damage, like thinning of the corneal epithelial layer, superficial punctate keratitis, corneal erosion, etc. In this study, we focused on looking for a more accessible light source and safer UVC wavelength, and 275-nm UVC LED light was chosen. We investigated its applicability for SSTIs therapy with relative skin safety and expected that it could be used as a new physical therapy method for SSTIs.
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 6
·202200862
·
IF 3.1
Jiana Lin,Xiaoyan Ouyang,Yuling Hu,Gongke Li,Qisheng Zhong
A highly chemically stable primary amide-functionalized cyclotricatechylene covalent organic framework was synthesized by an irreversible reaction and a post-synthetic modification. It possessed a rod-like morphology and exhibited strong solvent stability owing to the polyether bonds. The material showed good adsorption performance for melamine and its derivatives and adsorption mechanism was investigated by molecular simulations. The adsorbent was coated on the nylon-66 membrane to prepare the enrichment membrane. Under optimized conditions, an in-syringe membrane-based extraction method, combined with ultra-high performance liquid chromatography-tandem mass spectrometry was developed for the analysis of melamine and six melamine derivatives in the migration solution. A good linearity was obtained with correlation coefficients ranging from 0.9924 to 0.9995. The limits of detection were 1–200 ng/L and the limits of quantification were 3–500 ng/L. This method was successfully applied to the migration solution of sushi bamboo rolling mats with spiked recoveries of 73.2%–115% and relative standard deviations of 0.9%–9.9%. This work shows a practical and perspective approach for the efficient enrichment of food contact material hazards.
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高影响因子
ANALYST
·
2023
·Vol. 148
·期 18
·4339-4345
·
IF 4.2
Zhong-Xia Wang,Kai-Qi Liu,Xiang-Ying Meng,Feng Li,Heng-Ye Li,Hang Gao,Wei Wang
Meloxicam (Mel), as a powerful and effective anti-inflammatory drug, is commonly employed for the treatment of various inflammatory diseases; however, the use of Mel at high doses or for extended periods could cause severe side effects in human visceral organs. Therefore, a simple, rapid, and reliable method is urgently needed to monitor Mel in biological samples. Herein, novel water-soluble luminescent nano-carbon dots (nano-Cdots) with outstanding physicochemical properties were prepared by a one-pot high-temperature hydrothermal process of ellagic acid and guanidine. The nano-Cdots were further used as an optical probe for the sensitive detection of Mel in serum samples through the cooperative mechanisms of the inner filter effect and photoelectron transfer. By employing this sensor, an excellent linear correlation was achieved between the relative luminescent intensity [(PL0 − PL)/PL0] and the concentration of Mel in the range of 0.1 to 200 μM, with a limit of detection of 34.68 nM (3σ/k). This sensor was effectively employed for the analysis of Mel in real serum samples, implying its potential development prospects for the advancement of drug analysis with carbon-based probes.
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Pharmaceutics
·
2022
·Vol. 14
·期 9
·1937
·
IF 6.525
Chenghua Song,Ruichao Wen,Jiaxuan Zhou,Xiaoyan Zeng,Zi Kou,Jia Zhang,Tao Wang,Pengkang Chang,Yi Lv,Rongqian Wu
The increasing resistance of human pathogens promotes the development of novel antimicrobial agents. Due to the physical bactericidal mechanism of membrane disruption, antimicrobial peptides are considered as potential therapeutic candidates without inducing microbial resistance. Scorpion venom-derived peptide,Androctonus amoreuxiAntimicrobial Peptide 1 (AamAP1), has been proved to have broad-spectrum antimicrobial properties. However, AamAP1 can induce hemolysis and shows strong toxicity against mammalian cells. Herein, the antimicrobial activity and mechanism of a novel synthetic antimicrobial peptide, GK-19, derived from AamAP1 and its derivatives, was evaluated. Five bacteria and three fungi were used to evaluate the antimicrobial effects of GK-19 in vitro. Scalded mice models combined with skin and soft tissue infections (SSTIs) were used to evaluate its applicability. The results indicated that GK-19 could not only inhibit Gram-positive and Gram-negative bacterial growth, but also kill fungi by disrupting the microbial cell membrane. Meanwhile, GK-19 showed negligible toxicity to mammalian cells, low hemolytic activity and high stability in plasma. Furthermore, in scalded mice models combined with SSTIs induced by either Methicillin-ResistantStaphylococcus aureus(MRSA) orCandida albicans, GK-19 showed significant antimicrobial and healing effects. Overall, it was demonstrated that GK-19 might be a promising drug candidate in the battle against drug-resistant bacterial and fungal infections.Keywords:antimicrobial peptides;GK-19;skin and soft tissue infections;MRSA;Candida albicans
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Materials Today Energy
·
2022
·
IF 9.257
Xiaochun Hu,Yuqing Luo,Xianyue Wu,Jiabin Niu,Mingwu Tan,Zhiqiang Sun,Wen Liu
Heteroatom-doped microporous carbon nanosheets (HMCNs) are used in a wide range of applications, including gas adsorption, energy storage, and catalysis. Here, we demonstrate a solvent-free, template-free, one-pot polycondensation approach for the synthesis of HMCNs using melamine (MEL) and pentaerythritol (PER) as precursors. By varying the ratio of MEL to PER and the pyrolysis temperature, the doping amount, surface area and porosity of the HMCNs can be controlled. When used for CO 2 capture, the HMCN synthesized by the pyrolysis of a 3:2 mixture of MEL and PER at 900 °C (3/2-HMCNs-900) affords a CO 2 uptake of 5.35 mmol g −1 at 273 K and 1 bar CO 2 partial pressure. Density functional theory calculations suggest that the high CO 2 uptake performance of the HMCNs is associated with the chemical modification of the surface, as a result of N- and O- co-doping. When assembled in a supercapacitor , 3/2-HMCNs-900 exhibits a high specific capacitance (475 F g −1 at 1.3 A) and a fast charge-discharge rate of 13.3 F s −1 g −1 . This study presents a novel, resource-efficient and environmentally friendly method for preparing HMCNs for energy and environmental applications.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
·
IF 5.518
Ya Xu,Ru Zhou,Jingjing Mu,Yanming Ding,Juncheng Jiang
Aluminum diethylphosphinate (ADP) was surface modified with the silane coupling agent KH-560, and mixed intumescent flame retardants (IFRs) were prepared with neopentyl glycol (NPG) and melamine (MEL). Then mixed IFRs and zinc borate (ZB) were compounded and added into linear low-density polyethylene (LLDPE) material. The morphology, composition , and structure of LLDPE/IFRs/ZB composites were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy , and X-ray photoelectron spectroscopy. The maximum limiting oxygen index of LLDPE/IFRs/ZB composites is 28.7%, and the combustion grade is V 0. The thermogravimetric analysis results showed that the thermal degradation rate of LLDPE/IFRs/ZB composites decreased, and the carbon residue reached 15.3 wt% at 700 ℃. The cone calorimeter test showed that peak heat release rate (PHRR), total heat release rate (THR), peak smoke release rate (PSPR), and total flue gas output (TSP) of LLDPE/IFRs/ZB composites decreased by 52.5%, 24.7%, 30%, and 15.5%, respectively, compared with pure LLDPE, significantly improving the flame retardant and smoke suppression performances of LLDPE. The residual carbon analysis shows that the C C and P O C bonds in the modified mixed IFR strengthen the carbon layer structure and form a denser intumescent carbon layer, effectively hindering flame propagation. The experimental results show that the new intumescent flame retardant is a remarkable breakthrough in both flame retardancy and smoke suppression compared with ordinary intumescent flame retardants, and is more suitable for flame retardant polymer materials than traditional intumescent flame retardants.
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BIOMEDICINE & PHARMACOTHERAPY
·
2023
·
IF 7.5
Gilbert Audira,Jong-Chin Huang,Kelvin H.-C. Chen,Kevin Adi Kurnia,Ross D. Vasquez,Marri Jmelou M. Roldan,Yu-Heng Lai,Chung-Der Hsiao,Cheng-Yo Yen
Recently, the usage of zebrafish for pain studies has increased in the past years, especially due to its robust pain-stimulated behaviors. Fin amputation has been demonstrated to induce a noxious response in zebrafish. However, based on the prior study, although lidocaine, the most used painkiller in zebrafish, has been shown to ameliorate amputated zebrafish behaviors, it still causes some prolonged effects. Therefore, alternative painkillers are always needed to improve the treatment quality of fin-amputated zebrafish. Here, the effects of several analgesics in recovering zebrafish behaviors post-fin amputation were evaluated. From the results, five painkillers were found to have potentially beneficial effects on amputated fish behaviors. Overall, these results aligned with their binding energy level to target proteins of COX-1 and COX-2. Later, based on their sub-chronic effects on zebrafish survivability, indomethacin, and diclofenac were further studied. This combination showed a prominent effect in recovering zebrafish behaviors when administered orally or through waterborne exposure, even with lower concentrations. Next, based on the ELISA in zebrafish brain tissue, although some changes were found in the treated group, no statistical differences were observed in most of the tested biomarkers. However, since heatmap clustering showed a similar pattern between biochemical and behavior endpoints, the minor changes in each biomarker may be sufficient in changing the fish behaviors.
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高影响因子
DRYING TECHNOLOGY
·
2023
·
IF 3.3
Yanchao Li,Haosen Zhang,Tianwei Zhang,Tao Liu
This study aimed to produce nanoparticles with reduced crystallinity by using a combination method. The effect of solid-state transition (different drying processes) coupled with particle size reduction (homogenization) on drug dissolution was systematically investigated. Meloxicam, indomethacin and naproxen with different X-ray diffraction crystal intensity (relative high, medium and low) were selected as models. All drugs were first processed with and without polyvinylpyrrolidone (PVP) to obtain solid dispersions and then high pressure homogenization was used to produce nanosuspensions. Changes of particle morphology might be an important factor to improve the nanosizing efficiency. The smallest particle size (188 nm) could be obtained by using freeze-dried naproxen coupled with PVP after 20 homogenization cycles. Dissolution rates of processed indomethacin and meloxicam without PVP were found similar to their raw drugs. However, significant dissolution improvements were found after homogenization (higher than nanocrystals). Dissolution values of three drug nanoparticle achieved by using PVP dispersions were all higher than 83%. For naproxen, although nanosized solid dispersion possessed the smallest size and amorphous state, its dissolution curve was comparable to the larger sized nanocrystals. It was inferred that the relatively low crystal intensity of the initial compound could be the reason. Nanosuspensions of indomethacin and meloxicam prepared by using the two-step method showed optimized dissolution compared to their nanocrystals and amorphous solid dispersions.
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Science China-Chemistry
·
2023
·
IF 9.6
Zhao Haitao,Bian Guoqing,Xu Xiang,He Weiwei,Zhang Lifen,Cheng Zhenping
Developing a new type of photocatalyst (PC) and catalytic mechanism for near-infrared (NIR) photocontrolled reversible-deactivation radical polymerization (RDRP) system is charming but challenging. Herein, a novel PC of the persistent radical anion (PRA) (possessing the properties of both radical and anion) was developed for NIR photocontrolled reversible addition-fragmentation chain transfer (RAFT) polymerization, enabling successful polymerization while gaining a deep insight into the mechanism of photo-induced electron transfer RAFT (PET-RAFT) polymerization. Different from the conventional and well-accepted reductive quenching (RQ) pathway, in which the radical anion intermediates of PCs (PCs •− ) must be generated in an excited state (ES), here, the PRA (3,4,9,10-perylenetetracarboxylic dianhydride radical anion (PTCDA •− )) could generate conveniently in situ in the ground state (GS) and subsequently serve as highly efficient PC in the NIR region (740–850 nm). The successful implementation of this strategy elucidates the peculiar role played by light and the real way of electron transfer behaviors. In fact, the transfer of a single electron from PRA to chain transfer agent (CTA) and cleavage of the C–S bonds is a process from ES to GS, rather than always from GS (PCs •− ) to GS (CTA) in the RQ pathway as is well known to all. In addition, the excellent spatial-temporal control and powerful penetration ability of the NIR light were also confirmed by this PRA-catalyzed polymerization system.
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高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Wei Zhu,Xiaoqiang Feng,Zhiduo Liu,Menghan Zhao,Peng He,Siwei Yang,Shiwei Tang,Da Chen,Qinglei Guo,Gang Wang,Guqiao Ding
Surface-enhanced Raman scattering (SERS) substrates based on graphene and its derivatives have recently attracted attention among those interested in the detection of trace molecules; however, these substrates generally show poor uniformity, an unsatisfactory enhancement factor, and require a complex fabrication process. Herein, we design and fabricate three-dimensional (3D) graphene/silicon (3D-Gr/Si) heterojunction SERS substrates to detect various types of molecules. Notably, the detection limit of 3D-Gr/Si can reach 10–10 M for rhodamine 6G (R6G) and rhodamine B (RB), 10–7 M for crystal violet (CRV), copper(II) phthalocyanine (CuPc), and methylene blue (MB), 10–8 M for dopamine (DA), 10–6 M for bovine serum albumin (BSA), and 10–5 M for melamine (Mel), which is superior to most reported graphene-based SERS substrates. Besides, the proposed 3D-Gr/Si heterojunction SERS substrates can achieve a high uniformity with relative standard deviations (RSDs) of less than 5%. Moreover, the 3D-Gr/Si SERS substrates are reusable after washing with ethyl alcohol to remove the adsorbed molecules. These excellent SERS performances are attributed to the novel 3D structure and abundantly exposed atomically thin edges, which facilitate charge transfer between 3D-Gr and probe molecules. We believe that the 3D-Gr/Si heterojunction SERS substrates offer potential for practical applications in biochemical molecule detection and provide insight into the design of high-performance SERS substrates.
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