PROCESS BIOCHEMISTRY
·
2025
·
IF 4
Kit-Leong Cheong,Keying Liu,Suresh Veeraperumal,Gowsika Jaikumar,Sathuvan Malairaj,Xiaofei Liu,Xuejing Jia,Peiying Zheng,Hengyi Jiang,Ruixu Cai,Saiyi Zhong,Udayakumar Veerabagu
Laminarin, a polysaccharide derived from brown algae, has gained attention for its potential biological activities. However, its application in cosmetic formulations is limited due to its functional properties. To enhance its bioavailability and performance, laminarin was modified through esterification to form laminarin butyl esters (LBE). This study aimed to evaluate the antioxidant, skin-whitening, and anti-wrinkle properties of LBE and assess its potential as a novel ingredient in skincare products. Antioxidant activity was evaluated using in vitro assays, including DPPH and ABTS radical scavenging, both of which demonstrated significant free radical neutralizing capacity. Skin-whitening and anti-wrinkle activities were assessed by measuring the inhibition of tyrosinase and elastase. The results indicated that LBE exhibited significantly higher inhibitory effects than native laminarin, with tyrosinase inhibition reaching 26.7 % (compared to 4.7 % for laminarin) and elastase inhibition at 45.8 % (versus 18.5 % for laminarin). Additionally, LBE showed stronger anti-glycation activity, as it effectively inhibited Amadori products, dicarbonyl compounds, and advanced glycation end-products. These findings provide valuable insights into the modification of laminarin and its potential application in cosmetics, offering a foundation for the development of more effective, bioactive ingredients in the beauty industry.
研究领域:
化妆品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Cosmetic Dermatology
·
2023
·Vol. 23
·期 1
·244-255
·
IF 2.3
Xiufang Yan,Xiaoyu Ma,Daoxin Dai,Xiaojuan Yan,Xingyan Han,Xijun Bao,Qiufang Xie
Background Frankincense volatile oil (FVO) has long been considered a side product in pharmaceutical industry since frankincense of large molecular weight is the prime target. However, the volatile oil recycled in the extract process might contain a series of functional actives, serving as promising ingredients in the cosmetic field. Methods Gas chromatography-mass spectrometer was utilized to determine the species and amount of active ingredients in FVO. Subsequently, zebrafish models were used to evaluate pigmentation inhibition, ROS elimination and neutrophil activation. In vitro DPPH test was also conducted to consolidate the anti-oxidation efficacy. Based on the test results, network pharmacology was incorporated, where GO and KEGG enrichment analyses were performed to discover the interrelations between active ingredients. Results About 40 actives molecules were identified, including incensole, acetate incensole, and acetate incensole oxide. The FVO demonstrated great depigmentation activity by suppressing melanin synthesis, as well as providing free radical scavenging and anti-inflammation effect. In network pharmacology analysis, 192 intersected targets were identified. By enrichment analysis and network construction, a series of whitening signal pathways, and hub genes, containing STAT3，MAPK3，MAPK1 were identified. Conclusion The current study quantified the components of FVO, evaluated its efficacy in skin depigmentation, and give pioneering insights on the possible mechanism. The results confirmed that the FVO could serve as whitening agent in topical uses.
研究领域:
药理学
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化妆品科学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Materials
·
2023
·
IF 3.2
Cheng Yuxun,Fang Shishu,Liu Xulin,Li Xin,Song Zhixin,Ma Yanning,Jin Zuolin
Introduction: Clear aligner treatment (CAT) has become popular over recent years because it is both comfortable and aesthetically acceptable. However, most of patients undergoing orthodontic treatment request dental bleaching. A safe and controlled bleaching treatment at the same time as the clear aligner treatment can save time and improve patient satisfaction with the outcome of the treatment.Aim: This study was aimed to develop a thermoforming film loaded with hydrogen peroxide as a clear aligner and detect its efficiency on teeth blenching and its influence on shear bonding strength for attachment.Methods: The thermoforming film loaded with sodium alginate-dopamine/Mesoporous silica nanoparticles compound gel was immersed in 6 wt% hydrogen peroxide solution and the hydrogen peroxide was loaded into mesoporous silica nanoparticle channels by capillary action. Then, a thermoforming film loaded with sustained-release hydrogen peroxide gel was made. Six dentition models were prepared with 90 isolated human premolars and divided into the experiment group, the condition control group and the blank control group, respectively. Then, the experiment group wore the clear aligner made by the thermoforming film loaded with hydrogen peroxide for 40 days; the conditional control group wore the clear aligner made by the ordinary thermoforming film for 40 days; and the blank control group wore no clear aligner. The aligners were updated every 10 days and the color of teeth was measured every 10 days. Tooth color should be determined by specific parameters (L, a* and b*). What’s more, in order to determine the influence of the thermoforming film loaded with sustained-release hydrogen peroxide gel on shear bonding strength for attachment. The shear bonding strength of attachment of isolated premolars were measured.Results: Isolated premolars treated by bleaching experiments showed an increase in L value (ΔL = 7.76 ± 0.64) and a decrease in both a* (Δa = −0.82 ± 0.12) and b* (Δb = −3.10 ± 0.21) values. However, the isolated premolars in conditional control group and blank control group exhibited that an decrease in L value (ΔLCCG = −0.91 ± 0.24; ΔLBCG = −0.86 ± 0.15)and a increase in both a* (ΔaCCG = 0.19 ± 0.05; ΔaBCG = 0.18 ± 0.04) and b* (ΔbCCG = 0.43 ± 0.11; ΔbBCG = 0.31 ± 0.10) value. While the shear bonding strength for attachment after bleaching was 22.78 ± 2.28 MPa, which had no significant change compared with the shear bonding strength for attachment without bleaching experiment (22.21 ± 2.77 MPa) (p > 0.05). Conclusion: A thermoforming film featuring the sustained release of hydrogen peroxide had a good bleaching effect on isolated teeth and had no significant influence on the shear bonding strength for attachment.
研究领域:
牙科学
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材料科学
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口腔医学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Ginseng Research
·
2024
·
IF 6.8
Zeyu Wang,Daiyan Zhang,Mingju Shui,Ian Wa Ho,Weng Si Kou,Jianwen Wei,Jian-Bo Wan,Ruibing Wang,Qing-Wen Zhang
Background Ginsenoside, as an active ingredient in traditional Chinese medicine, has been widely used for skin whitening for several years. Recent research has found that Panax notoginseng has a higher content of ginsenosides compared with the Panax ginseng. Those ginsenosides have promising potential to be developed as skin whitening agents. Methods We selected five dammarane ginsenosides isolated from P. notoginseng and their mixtures to investigate the skin lightning activity. Zebrafish embryo model was used for initial screening of the whitening activity. Subsequently, the whitening effect of components was examined and compared via testing the inhibition of melanin and activity of tyrosinase in B16 cells treated with these components. Molecular docking was also applied to investigate the interactions between ginsenosides and tyrosinase. Finally, the most effective saponins were selected for dosage form optimization and the whitening effect of saponin-loaded ethosomes was further demonstrated on the C57BL/6 mouse model. Results Experimental results showed that the protopanaxtriol saponins (PTS) were the most potent saponins with a decent safety profile, and the molecule docking results demonstrated that PTS had strong inhibitory ability to tyrosinase. PTS was successfully encapsulated into ethosomes with an encapsulation efficiency of 93%. The PTS ethosome gel could effectively inhibit the melanin production caused by UVB tanning on the back skin of mice. Conclusion The PTS ethosome gel provides an effective and safe formulation of PTS to whiten the UVB-tanned skin in vivo and could be used as a potential skin whitening agent in the future.
研究领域:
药理学
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皮肤科学
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中药学
✓ 已验证
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2024
·
IF 3.7
Yanting Xu,Jing Zhuang,Shuo Wang,Jianying Dai,Zhi-Long Xiu
The aim of this work was to develop an integrated process of 2,3-butanediol production and extraction of active components from Chinese herbs via fermentation, and evaluate their potential as cosmetic ingredients in vitro. A GRAS strain DL2–2 of Bacillus licheniformis was isolated and the fermentation medium was optimized using the ingredients that could be used in cosmetics. Four types of Chinese herbs were added solely or in combination into the fermentation medium to perform 2,3-butanediol production and active components extraction simultaneously. The release of polysaccharides and flavonoids was promoted, and the antioxidant, whitening and antibacterial abilities were improved. The highest antioxidant and antibacterial activity was obtained from the ferment filtrate of Dendrobium devonianum and Gastrodia elata f. glauca , resulting from the highest contents of flavonoids (0.44 g/L) and polyphenols (0.31 g/L), and 42.2 g/L 2,3-butanediol was also produced. The highest polysaccharide concentration was obtained from the ferment filtrate containing Polygonatum kingianum , which exhibited the highest inhibitory effect on tyrosinase (65 %), similar to that of 1 g/L arbutin. Therefore, the filtrate of 2,3-butanediol-Chinese herbal fermentation broth is promising as an ingredient in the production of natural high-value cosmetics.
研究领域:
生物技术
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化妆品科学
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微生物学
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产品关联
DOI
高影响因子
DENTAL MATERIALS JOURNAL
·
2022
·Vol. 41
·期 5
·731-740
·
IF 2.418
Jae-Heon KIM,Soyeon KIM,Van Mai TRUONG,Joo Won LEE,Young-Seok PARK
The relative dentin abrasivity-profilometry equivalent values were compared using non-contact profilometry with three subtypes of regular toothpaste and two subtypes of whitening toothpaste containing sodium bicarbonate and 35% hydrogen peroxide. Bovine dentin specimens were assigned to six groups: regular toothpaste (R): R1 (BAMBOO SALT GUM OINTMENT); R2 (MEDIAN TARTAR ORIGINAL); R3 (PERIOE Alpha), Reference slurry: RS (calcium pyrophosphate), whitening toothpaste (W): W1 (NET. WT); W2 (Vussen 28 WHITENING). Relative dentin abrasion–profilometry equivalent (RDA-PE) was determined by brushing 10,000 times (n=8). The pH of the toothpaste was measured (n=5) and the abrasive constituents of the toothpaste was analyzed by FE-SEM and EDS. The RDA-PE values ranged from 26 to 166, and the pH level ranges were 4.928–9.153. The RDA-PE value of the whitening toothpaste containing hydrogen peroxide was not high compared with that of the regular toothpaste. The RDA-PE values of whitening toothpaste could vary depending on the mechanism and ingredients of the whitening agents.
研究领域:
口腔医学
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
AMERICAN JOURNAL OF DENTISTRY
·
2024
·Vol. 37
·期 1
·3-8
·
IF 0.9
Soyeon Kim,Yeon-Jee Yoo,Franklin Garcia-Godoy,Young-Seok Park
Purpose: To evaluate the effectiveness of whitening toothpaste in restoring tooth color after coffee staining and its potential impact on enamel surfaces compared with regular toothpaste. Methods: Bovine tooth enamel specimens were prepared and stained with coffee solutions before undergoing brushing simulation with different toothpaste slurries (whitening, regular, reference). For precise evaluation, spectrophotometric measurements were taken at intervals to assess color changes using the CIELAB (Commission Internationale de l'Éclairage Lab*) color space. Additionally, profilometric measurements were taken to determine the impact of toothpaste type on the roughness and abraded depth of the enamel surface. To understand the effects of toothpaste and brushing on color change, surface roughness, and abraded depth, while also considering correlations between these factors, the findings were analyzed using mixed-effects models. Results: The whitening toothpaste group demonstrated the highest recovery rate (71%) after 10,000 brushstrokes, followed by the regular toothpaste group (48%) and the reference slurry group (43%). The mixed-effects model analysis revealed that the reference group had a smaller change in lightness (ΔL) than those in the regular toothpaste group. The whitening toothpaste group showed a greater change in lightness on average than those in the regular toothpaste group, with an increase in lightness as the number of brushstrokes increased. According to the roughness and abraded depth data, the whitening toothpaste group was least affected by brushing, while the reference and regular toothpaste groups showed higher levels of roughness and abraded depth at all intervals. Clinical significance: Gaining a thorough understanding of the effectiveness of whitening toothpaste and its impact on the enamel surface plays a crucial role in refining toothpaste formulations and advancing tooth whitening techniques in dental care.
研究领域:
口腔医学
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2021
·Vol. 19
·期 1
·e202100740
·
IF 2.408
Xiang Liu,Jinqiu Rao,Kai Wang,Meijun Wang,Tie Yao,Feng Qiu
Many stilbene glycosides can alleviate skin hyperpigmentation due to their inhibitory effect on tyrosinase. Mulberrosides in Morus alba L. are stilbene glycosides. In the present study, the inhibition of tyrosinase by five mulberrosides (S1–5), isolated from Morus alba L. was investigated and compared, and the inhibitory mechanism was explored. These five mulberrosides exhibited obvious inhibitory effects on tyrosinase only in a concentration-dependent manner, without time-dependence, indicating that they are reversible inhibitors of tyrosinase. S2, S1 and S5 inhibited tyrosinase activity with IC 50 values of 28.93, 75.94 and 151.72 μM, respectively, and were more active than kojic acid (IC 50 =169.13 μM). Kinetic studies revealed that S1, S2 and S4 were competitive inhibitors, while S3 and S5 were mixed inhibitors. Analysis of the fluorescent spectra showed that mulberrosides S1, S2 and S4 quenched the intrinsic fluorescence intensity of tyrosinase. A molecular docking study indicated that the interaction of tyrosinase with mulberrosides was reflected by compound scores as follows: S2>S5>S1>S3/S4>kojic acid, and hydroxy groups in the side chain of mulberrosides may play a crucial role in the binding of the enzyme. Our results suggest that mulberrosides in Morus alba L. could be further developed as whitening agents for enhanced performance against hyperpigmentation. Graphical
研究领域:
植物化学
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生物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Haixia Xu,Xiaofeng Li,Xuan Xin,Lan Mo,Yucong Zou,Guanglei Zhao
In this paper, an enzymatic route for synthesizing phenolic glycoside azelaic acid esters was successfully set up via lipase-catalyzed esterification and transesterification. Among the lipases tested, Candida antarctica lipase B (Novozyme 435) showed the highest activity in catalyzing esterification and Thermomyces lanuginosus (Lipozyme TLIM) gave the highest substrate conversion in catalyzing transesterification for the synthesis of ester. The addition of 4A molecular sieves into the reaction system is found to be an effective method for in situ absorption of the byproduct water and methanol, with which the substrate conversions of the enzymatic esterification and transesterification were 98.7 and 95.1%, respectively. Also, the main product ratios in transesterification were above 99.0% with lipozyme TLIM as a catalyst because the hydrolysis reaction was hindered. The results of the physical and biological properties indicate that all esters had higher Clog p values than their parent compounds. Also, the esters showed higher intracellular tyrosinase inhibitory and depigmentating activities than phenolic glycosides, azelaic acid (AA), and their physical mixtures due to their higher membrane penetration and tyrosinase inhibitory effects. In particular, piceid 6″-O-azelaic acid ester (PIA) showed the strongest inhibitory effect against melanin production. Its inhibitory rate was 77.4% at a concentration of 0.25 mM, about 4.2 times higher than that of arbutin (18.5%).
研究领域:
生物化学
·
药理学
·
化妆品科学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2015
·
IF 1.03
Rong-Xiang Chen,Li Wang,Jie Wang,Fu-Qiang Xu
A simple method was developed for the determination of hydrophilic whitening agents (ascorbyl glucoside, magnesium ascorbyl phosphate, arbutin, hydroquinone, and kojic acid) in bleaching cosmetics by high performance liquid chromatography with electrochemical detection. The samples were prepared using microdialysis and were separated within ten minutes using isocratic elution. Linear calibration was achieved for concentrations between 0.10 and 10.00 micromolar. The limits of detection were from 0.6 to 2.0 nanomolar; the recoveries were between 91.43 and 108.04 percent, and the relative standard deviation was less than 6.88 percent. This method was used to determine whitening compounds in several cosmetics with on-line microdialysis and the zero net flux method. The procedure was fast, simple, selective, and suitable for routine analysis.
研究领域:
分析化学
·
化妆品科学
✓ 已验证
DOI
高影响因子
Nature Communications
·
2020
·Vol. 11
·期 1
·1-11
·
IF 12.121
Wang Yang,Wen Xinrong,Jia Yanmin,Huang Ming,Wang Feifei,Zhang Xuehui,Bai Yunyang,Yuan Guoliang,Wang Yaojin
The increasing demand for a whiter smile has resulted in an increased popularity for tooth whitening procedures. The most classic hydrogen peroxide-based whitening agents are effective, but can lead to enamel demineralization, gingival irritation, or cytotoxicity. Furthermore, these techniques are excessively time-consuming. Here, we report a nondestructive, harmless and convenient tooth whitening strategy based on a piezo-catalysis effect realized by replacement of abrasives traditionally used in toothpaste with piezoelectric particles. Degradation of organic dyes via piezo-catalysis of BaTiO 3 (BTO) nanoparticles was performed under ultrasonic vibration to simulate daily tooth brushing. Teeth stained with black tea, blueberry juice, wine or a combination thereof can be notably whitened by the poled BTO turbid liquid after vibration for 3 h. A similar treatment using unpoled or cubic BTO show negligible tooth whitening effect. Furthermore, the BTO nanoparticle-based piezo-catalysis tooth whitening procedure exhibits remarkably less damage to both enamel and biological cells.
研究领域:
牙医学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2022
·Vol. 11
·期 4
·772
·
IF 7.675
Jiaman Liu,Yanbiao Chen,Xinxin Zhang,Jie Zheng,Weiying Hu,Bo Teng
The application of hops could be extended to obtain higher commercial values. Tannins from hops were assessed for their tyrosinase inhibition ability, and the associated mechanisms were explored. Nuclear magnetic resonance (NMR) and high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (HPLC–ESI–MS/MS) revealed that the hop tannins were characterized as condensed tannins with (epi)catechin and (epi)gallocatechin as subunits and an average polymerization degree of 10.32. Tyrosinase inhibition assay indicated that hop tannins had an IC50= 76.52 ± 6.56 μM. Kinetic studies of the inhibition processes indicated the tannins provided inhibition through competitive–uncompetitive mixed reactions. In silico molecule docking showed that tannins were bound to the active site of tyrosinase via hydrogen and electrovalent bonds. Circular dichroism (CD) observed the structural variation in the tyrosinase after reacting with the tannins. Fluorescence quenching analysis and free radical scavenging assays indicated that the tannins had copper ion chelating and antioxidant activities, which may also contribute to inhibition. The intracellular inhibition assay revealed that the melanin was reduced by 34.50% in B16F10 cells. These results indicate that these tannins can be applied as whitening agents in the cosmetics industry.Keywords:hop tannins;polyphenols;condensed tannin;tyrosinase inhibitor;whitening agent
研究领域:
药理学
·
化妆品学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）