Gels
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2025
·Vol. 11
·期 4
·285
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IF 5.3
Lin Zhang,Xiaoling Lu,Hao Li,Hongjie Chen,Qi Yin,Xuehan Hu,Kang Yang,Fang Yang,Liya Chen,Zeng Yang,You Long,Chao Shen,Bo Yao,Chenghong Huang
The medicinal value of vanillin and its isomers has not been well developed, so it is necessary to prepare crystals of vanillin and its isomers as well as to investigate their crystallization rules in detail using advanced crystallization techniques in polymer gel. Based on molecular simulation, the maximum number of hydrogen bonds between CMCS with Van, IsoVan and oVan were reached at molar ratios of 1:9 and 1:4 and 1:5, respectively. The gel hardness and apparent viscosity of CMCS/Van isomers were proportional to the mole ratio, while elongation at break and tensile strength decreased with an increase in molar concentration depending on the position of the side chain group of the Van isomer, exposure of the benzene ring, steric resistance and the number of hydrogen bonds formed. The crystallization of Van, IsoVan and oVan in CMCS gel unexceptionally follow classical supersaturation theory in the case that nVan mainly exhibits a unique growth pattern from needle to strip, IsoVan’s growth style changes from plate to bulk and oVan adapts growth pattern from needle to branch bifurcating. It was also found that the Van crystal changed from II-type to I-type under long-term heating. Studies have further confirmed that the discrepancy of physicochemical characteristics of CMCS/Van blend gel can be attributed to differences in the number of hydrogen bonds compared to CMCS with given group positions of Van isomers. This study provides powerful technical support for the gel crystallization of van isomers.
研究领域:
材料科学
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药剂学
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高分子化学
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分子模拟
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PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2022
·
IF 3.141
YuLiang Jiao,YuYing Zhu,ShuMin Zeng,ShuFang Wang,Jing Chen,XiangHong Zhou,GuiZhen Ma
A novel uricase producing marine bacterium Priestia flexa alkaAU was isolated and identified. The 16S rDNA and the uricase coding gene were sequenced, analyzed and submitted to GenBank. The uricase from Priestia flexa alkaAU (PFU) was purified, determined to be 58.87 kDa, and conjugated with carboxymethyl chitosan (CMCS) by ionic gelation. CMCS conjugation had no effect on the optimum pH of PFU but decreased the optimum temperature by 10 °C. CMCS conjugation increased the specific activity of PFU by 53% at the human body temperature (37 °C) and small intestine’s pH (pH 6.8). Uricase thermostabilizing ability of CMCS was significant in the range of 37–80 °C but not at lower temperatures. For improvement of the pH stability of PFU, CMCS was more effective at pHs 3–5 than pHs 6–11. CMCS increased the half-life of PFU against artificial intestinal fluid by 1.5 folds, which demonstrated the potential capability of CMCS–PFU for oral administration.
研究领域:
海洋微生物学
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酶学
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生物技术
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药学
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DOI
PubMed
高影响因子
CURRENT MICROBIOLOGY
·
2023
·Vol. 80
·期 9
·1-9
·
IF 2.6
Jiao Yu Liang,Shen Pin Quan,Wang Shu Fang,Chen Jing,Zhou Xiang Hong,Ma Gui Zhen
Arginase has shown promising potential in treating cancers by arginine deprivation therapy; however, low enzymatic activity and stability of arginase are impeding its development. This study was aimed to improve the enzymological properties of a marine bacterial arginase by carboxymethyl chitosan (CMCS) conjugation. An arginase producing marine bacterium Priestia megaterium strain P6 was isolated and identified. The novel arginase PMA from the strain was heterologously expressed, purified, and then conjugated to CMCS by ionic gelation with calcium chloride as the crosslinking agent. Enzymological properties of both PMA and CMCS-PMA conjugate were determined. The optimum temperature for PMA and CMCS-PMA at pH 7 were 60 °C and 55 °C, respectively. The optimum pH for PMA and CMCS-PMA at 37 °C were pH 10 and 9, respectively. CMCS-PMA showed higher thermostability than PMA over 55–70 °C and higher pH stability over pH 4–11 with the highest pH stability at pH 7. At 37 °C and pH of 7, i.e., around the human blood temperature and pH, CMCS-PMA was higher than the free PMA in enzymatic activity and stability by 24% and 21%, respectively. CMCS conjugation not only changed the optimum temperature, optimum pH, and enzymatic activity of PMA, but also improved its pH stability and temperature stability, and thus made it more favorable for medical application.
研究领域:
生物化学
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生物技术
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药学
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癌症研究
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 46
·期 4
·1569-1576
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IF 3.591
Zhuoyao Ni,Jiajie Hu,Hui Zhu,Yazhuo Shang,Daijie Chen,Yinghong Chen,Honglai Liu
Antibiotic resistance caused by long-term abuse of antibiotics has inevitably become a very serious problem and developing novel strategies to enhance the efficacy of treatments is exigent. Herein, a dual-antibacterial platform using sodium alginate (SA) and carboxymethyl chitosan (CMCS) as the framework was designed for NIR controlled pharmacotherapy and photothermal therapy of drug-resistant bacteria. The SA/CMCS solution can quickly transform into a hydrogel in response to Ca2+ and Mg2+ in biological bodies. By mixing indocyanine green (ICG) and thermosensitive drug-loaded liposomes into the solution, this designed platform can not only achieve near-infrared (NIR) controlled drug release properties but also possess dual-antibacterial capacity by combining pharmacotherapy and photothermal therapy (PTT). Local temperature rise induced by ICG will break the stability of thermosensitive liposomes, causing the release of the encapsulated drug. The in vitro antibacterial test and in vitro cytotoxicity experiment demonstrate the excellent antibacterial capacity and satisfactory biocompatibility of the prepared platform. The proposed NIR controlled dual-antibacterial platform can not only avoid bacterial resistance but also kill drug-resistant bacteria efficiently, which will provide a novel concept for the development of an intelligent antibacterial platform.
研究领域:
药学
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生物医学工程
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材料科学
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DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2023
·
IF 5.8
Puxin Lang,Tiantian Liu,Shiqi Huang,Zhaojie Zhou,Mengxing Zhang,Yunzhu Lin,Qin He,Yuqin Yao,Zhenmi Liu,Ling Zhang
Tissue adhesions could occur following surgeries, and severe tissue adhesions can lead to serious complications. Medical hydrogels could be applied at surgical sites as a physical barrier to prevent tissue adhesion. For practical reasons, spreadable, degradable, and self-healable gels are highly demanded. To meet these requirements, we applied carboxymethyl chitosan (CMCS) to poloxamer-based hydrogels to generate low Poloxamer338 (P338) content gels displaying low viscosity at refrigerator temperature and improved mechanical strength at body temperature. Heparin, an effective adhesion inhibitor, was also added to construct P338/CMCS-heparin composite hydrogel (PCHgel). PCHgel presents as a flowable liquid below 20 °C and could rapidly transform into gel when spread on the surface of damaged tissue due to temperature change. The introduction of CMCS enabled hydrogels to form a stable self-healable barrier at injured positions and slowly release heparin during the wound healing period before being degraded after ∼14 days. Ultimately, PCHgel significantly reduced tissue adhesion in model rats and displayed higher efficiency than P338/CMCS gel without heparin. Its adhesion suppression mechanism was verified, and it also displayed good biosafety. Therefore, PCHgel showed good clinical transformation potential with high efficacy, good safety, and ease of use.
研究领域:
生物医学工程
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材料科学
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外科研究
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药剂学
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DOI
PubMed
高影响因子
Polymers
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2023
·Vol. 15
·期 12
·2744
·
IF 5
Yue Jiang,Shanzhu Guo,Jingjing Jiao,Long Li
The treatment of osteochondral defects remains challenging due to the limited healing capacity of cartilage and the poor results of traditional methods. Inspired by the structure of natural articular cartilage, we have fabricated a biphasic osteochondral hydrogel scaffold using a Schiff base reaction and a free radical polymerization reaction. Carboxymethyl chitosan (CMCS), oxidized sodium alginate (OSA), and polyacrylamide (PAM) formed a hydrogel (COP) as the cartilage layer, while hydroxyapatite (HAp) was incorporated into the COP hydrogel to obtain a hydrogel (COPH) as an subchondral bone layer. At the same time, hydroxyapatite (HAp) was incorporated into the COP hydrogel to obtain a hydrogel (COPH) as an osteochondral sublayer, combining the two to obtain an integrated scaffold for osteochondral tissue engineering. Interlayer interpenetration through the continuity of the hydrogel substrate and good self-healing properties due to the dynamic imine bonding of the hydrogel resulted in enhanced interlayer bond strength. In addition, in vitro experiments have shown that the hydrogel exhibits good biocompatibility. It shows great potential for osteochondral tissue engineering applications.Keywords:biphasic scaffolds;self-healing hydrogel;osteochondral repair
研究领域:
组织工程
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生物材料
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骨科
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Na Wen,Shuangshuang Li,Hongzhi Jiang,Jiachao Yang,Weibo Yang,Yunhao Song,Jinlin Long,Jiwu Zhao,Zhihui Lin,Xunbin Yu,Yen Wei,Shiyun Lu,Xueping Huang,Tianhua Zhou
Gastric ulcers accompanied by acute or chronic bleeding represent a significant risk of fatality. However, the development of effective strategies for achieving rapid hemostasis and wound healing in gastric ulcer bleeding represents a formidable challenge. To address this challenge, thrombin-derived C-terminal peptide (TCP-25) is employed as a targeted agent, and it is incorporated into two novel injectable and biocompatible carboxymethyl chitosan (CMCs) hydrogels via Schiff's base reaction. The TCP-25 peptide hydrogels display optimal adaptability within the distinctive gastrointestinal microenvironment, demonstrating a balance of acid resistance and degradation, along with controlled release and bioactivity, and good bio-adhesive properties. Both in vitro and in vivo reveal that the hydrogel achieves rapid hemostasis. In an ethanol-induced gastric ulcer model in rats, it demonstrates that the hydrogel effectively inhibits gastric ulcer bleeding by 92% within 24-h, exhibiting efficacy that surpasses that of omeprazole medicine, a commonly used clinical treatment. This enhanced performance is attributed to the synergistic effects of the CMCs hydrogel and TCP-25 peptide in effectively achieving hemostasis, inhibiting bacterial growth, and promoting gastric wound healing in acute or chronic bleeding gastric ulcers. Consequently, the present work offers promising clinical applications for rapid hemostasis and wound healing in gastric ulcers.
研究领域:
生物医学工程
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材料科学
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药学
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胃肠病学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Shanshan Jin,Rajib Mia,Md All Amin Newton,Hongju Cheng,Weihong Gao,Yuansheng Zheng,Zijian Dai,Jie Zhu
The escalating global health concern arises from chronic wounds induced by bacterial infections, posing a significant threat to individuals. Consequently, an imperative exist for the development of hydrogel dressings to facilitate prompt wound monitoring and efficacious wound management. To this end, pH-sensitive bromothymol blue (BTB) and pH-responsive drug tetracycline hydrochloride (TH) were introduced into the polysaccharide-based hydrogel to realize the integration of wound monitoring and controlled treatment. Polysaccharide-based hydrogels were formed via a Schiff base reaction by cross-linking carboxymethyl chitosan (CMCS) on an oxidized sodium alginate (OSA) skeleton. BTB was used as a pH indicator to monitor wound infection through visual color changes visually. TH could be dynamically released through the pH response of the Schiff base bond to provide effective treatment and long-term antibacterial activity for chronically infected wounds. In addition, introducing polylactic acid nanofibers (PLA) enhanced the mechanical properties of hydrogels. The multifunctional hydrogel has excellent mechanical, self-healing, injectable, antibacterial properties and biocompatibility. Furthermore, the multifaceted hydrogel dressing under consideration exhibits noteworthy capabilities in fostering the healing process of chronically infected wounds. Consequently, the research contributes novel perspectives towards the advancement of intelligent and expeditious bacterial infection monitoring and dynamic treatment platforms.
研究领域:
生物医学工程
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材料科学
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伤口护理
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药学
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组织工程
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DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
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2020
·
IF 4.152
Wanjun Hu,Zhigang Zhang,Long Zhu,Yazhou Wen,Tianzhu Zhang,Pengfei Ren,Faming Wang,Zhenling Ji
Polypropylene (PP) mesh has been used successfully for a long time in clinical practice as an impressive prosthesis for ventral hernia repair. To utilize a physical barrier for separating mesh from viscera is a general approach for preventing adhesions in clinical practice. However, a serious abdominal adhesion between the mesh and viscera can possibly occur post-hernia, especially with the small intestine; this can lead to a series of complications, such as chronic pain, intestinal obstruction, and fistula. Thus, determining how to prevent abdominal adhesions between the mesh and viscera is still an urgent clinical problem. In this study, a dopamine-functionalized polysaccharide derivative (oxidized-carboxymethylcellulose-g-dopamine, OCMC-DA) was synthesized; this was blended with carboxymethylchitosan (CMCS) to form a hydrogel (OCMC-DA/CMCS) in situ at the appropriate time. The physical and chemical properties of the hydrogel were characterized successfully, and its excellent biocompatibility was presented by the in vitro cell test. The combination of this hydrogel and PP mesh was used in laparoscopic surgery for repairing the abdominal wall defect, where the hydrogel could become fixed in situ on the PP mesh to form an anti-adhesion gel-mesh. The results showed that the gel-mesh could prevent abdominal adhesions effectively in the piglet model. Moreover, the histology and immunohistochemical staining proved that the gel-mesh could effectively alleviate the inflammation reaction and deposition of collagen around the mesh, and it did not disturb the integration between mesh and abdominal wall. Thus, the gel-mesh has superior tissue compatibility.
研究领域:
手术材料
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生物材料
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组织工程
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医疗器械
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外科技术
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动物模型
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Zuoxiang Dong,Jian Xu,Peng Lun,Zeyu Wu,Wenshuai Deng,Peng Sun
Given the widespread clinical demand, addressing irregular cranial bone defects poses a significant challenge following surgical procedures and traumatic events. In situ-formed injectable hydrogels are attractive for irregular bone defects due to their ease of administration and the ability to incorporate ceramics, ions, and proteins into the hydrogel. In this study, a multifunctional hydrogel composed of oxidized sodium alginate (OSA)-grafted dopamine (DO), carboxymethyl chitosan (CMCS), calcium ions (Ca2+), nanohydroxyapatite (nHA), and magnesium oxide (MgO) (DOCMCHM) was prepared to address irregular cranial bone defects via dynamic Schiff base and chelation reactions. DOCMCHM hydrogel exhibits strong adhesion to wet tissues, self-healing properties, and antibacterial characteristics. Biological evaluations indicate that DOCMCHM hydrogel has good biocompatibility, in vivo degradability, and the ability to promote cell proliferation. Importantly, DOCMCHM hydrogel, containing MgO, promotes the expression of osteogenic protein markers COL-1, OCN, and RUNX2, and stimulates the formation of new blood vessels by upregulating CD31. This study could provide meaningful insights into ion therapy for the repair of cranial bone defects.
研究领域:
材料科学
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生物材料
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组织工程
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生物医学工程
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骨科
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再生医学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jingjing Yuan,Menghan He,Jueying Yang,Kang Li,Kexiang Fan,Hang Luo,Bo Li,Yu Chen
Femur fracture becomes increasingly common due to global population aging. Intramedullary nailing, a widely used treatment for fractures, tends to damage the blood vessels in the bone marrow cavity, which causes substantial hidden bleedings. The application of hydrogels to intramedullary nail hemostasis is limited by the challenges in balancing the adhesion, mechanical strength, and toughness. Inspired by the multi-network structure of snail mucus, which offers strong adhesion in harsh environments via adaptive wet-state contact followed by tight fitting upon dehydration, we developed a dual-network carboxymethyl chitosan (CMCS)-gluconic acid δ-lactone (GDL)-Ca 2+ /polyacrylamide (PAM) (CGC/P) hydrogel. The integration of GDL with CMCS introduces hydroxyl groups to enhance the adhesion of hydrogel and regulated system pH to facilitate the formation of the dynamic CMCS-GDL-Ca 2+ physical crosslinking network. The chemically crosslinked PAM network further improves the strength and toughness of the hydrogel. As the adhesion strength of the hydrogel can be increased by up to 50.14 times upon dehydration, a stretch-winding and dehydration method is developed to coat intramedullary nails with controllable thickness, effective hemostatic properties, excellent mechanical and adhesion characteristics. In rabbit femoral intramedullary nailing surgery, this system achieves rapid hemostasis and greatly promotes osteogenesis. Our study simultaneously improves the performance regulation contradiction of hydrogel coating, providing a crucial support to promote the application of hemostatic coatings to implantations.
研究领域:
生物医学工程
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材料科学
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组织工程
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骨科
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生物材料
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）