ANALYTICAL CHEMISTRY
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2020
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IF 6.785
Li Fu,Bin Zhang,Kena Fu,Xuwen Gao,Guizheng Zou
Electrochemiluminescence (ECL) with high electrode compatibility and less electrochemical interference has conventionally been envisioned by lowering the oxidative potential of luminophores and/or screening luminophores with a low oxidative potential. Herein, an alternative was developed by employing the environmental-friendly carbohydrazide as a coreactant, which enabled serial luminophores with oxidative-reduction ECL at one similar low triggering potential around 0.55 V versus Ag/AgCl, including Ru(bpy)32+ as well as CdTe, CdSe, CuInS2/ZnS, and Au nanocrystals. Because the eight-electron releasing process of carbohydrazide was electrochemically triggered at ∼0.25 V versus Ag/AgCl, the radicals generated via electrochemical oxidation of carbohydrazide could reduce the luminophores at a much lower potential than those of traditional coreactants. All the luminophore/carbohydrazide systems exhibited one ECL process around 0.55 V, which was about 0.65 V lower than that of a traditional Ru(bpy)32+/tri-n-propylamine system (typically around +1.2 V), and even lower than the oxidative potential of some luminophores. The ECL of the luminophore/carbohydrazide system was spectrally close to that of the corresponding luminophore/tri-n-propylamine system; the maximum emission wavelength of the low triggering potential ECL could shift from 540 to 783 nm via the selection of luminophores in this case. The coreactant screening strategy would be a favorable addition to the expected luminophore screening strategy for achieving enhanced ECL performance. This work created an avenue toward a deeper understanding of the ECL mechanism.
研究领域:
分析化学
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电化学
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材料科学
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2017
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IF 3.359
Sheng Xu,Lifen Liu,Yan Wang
Amine cross-linking is one of the popular modifications of polyimide membranes in order to improve their operation stability and separation performance. In this work, a novel kind of cross-linker tricarbohydrazide — 1,3,5-benzenetricarboxylic acid trihydrazide (BTCH), is synthesized and incorporated into polyimide membranes for isopropanol dehydration via pervaporation. The chemical structure of BTCH is examined by NMR and FTIR to confirm the successful synthesis. The effects of BTCH content and membrane-forming temperature on the membrane physicochemical properties and separation performance are studied and characterized by various techniques. And the possible reaction mechanism during the chemical cross-linking is proposed and analyzed. This work is believed to shed useful insights on the chemical modification of polyimide membranes for pervaporation and other membrane-based separation applications.
研究领域:
材料科学
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分离技术
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
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2019
·Vol. 9
·期 12
·6717-6723
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IF 3.049
Xiuli Dong,Jiayuan Tang,Yan Ren,Xiangming Chen
A novel fluorescence labeling reagent N-acetylhydrazine acridone (AHAD) was designed and synthesized. A highly sensitive high performance liquid chromatography (HPLC) method coupled with fluorescence detection to determine benzaldehyde after derivatization with AHAD was developed. Optimum derivatization was obtained at 40 °C for 30 min with trichloroacetic acid as catalyst. Benzaldehyde derivative was separated on a reversed-phase SB-C18 column in conjunction with a gradient elution and detected by fluorescence detection at excitation and emission wavelengths of 371 nm and 421 nm. The established method exhibited excellent linearity over the injected amount of benzaldehyde of 0.003 to 5 nmol mL−1. The method was successfully applied to the determination of serum semicarbazide-sensitive amine oxidase (SSAO) activity in humans. SSAO is a significant biomarker because serum SSAO activity is elevated in patients with Alzheimer's disease, vascular disorders, heart disease and diabetes mellitus. It was demonstrated that the SSAO activity of the hyperglycemic group (60 ± 4 nmol mL−1 h−1) was significantly higher than that of normal blood sugar group (44 ± 4 nmol mL−1 h−1) with P < 0.05.
研究领域:
分析化学
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医学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
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2024
·
IF 5.6
Nie Xiongfeng,Tang Yong,Wu Tengling,Zhao Xinrui,Xu Ziyang,Yang Rong,Sun Yage,Wu Bin,Han Quanhong,Hui Jingwen,Liu Wenguang
3D printing of high-strength natural polymer biodegradable hydrogel scaffolds simultaneously resembling the biomechanics of corneal tissue and facilitating tissue regeneration remains a huge challenge due to the inherent brittleness of natural polymer hydrogels and the demanding requirements of printing. Herein, concentrated aqueous solutions of gelatin and carbohydrazide modified alginate (Gel/Alg-CDH) are blended to form a natural polymer hydrogel ink, where the hydrazides in Alg-CDH are found to form strong hydrogen-bonds with the gelatin. The hydrogen-bonding-strengthened Gel/Alg-CDH hydrogel demonstrates an appropriate thickened viscosity and shear-thinning for extrusion printing. The strong hydrogen bonds contribute to remarkably increased mechanical properties of Gel/Alg-CDH hydrogel with a maximum elongation of over 400%. In addition, sequentially Ca2+-physical crosslinking and then moderately chemical crosslinking significantly enhance the mechanical properties of Gel/Alg-CDH hydrogels that ultimately exhibit an intriguing J-shaped stress-strain curve (tensile strength of 1.068 MPa and the toughness of 677.6 kJ/m2). The dually crosslinked Gel-Alg-CDH-Ca2+-EDC hydrogels demonstrate a high transparency, physiological swelling stability and rapid enzymatic degradability, as well as suturability. Growth factor and drug-loaded biomimetic bilayer hydrogel scaffold is customized via a multi-nozzle printing system. This bioactive bilayer hydrogel scaffold considerably promotes regeneration of corneal epithelium and stroma, and inhibits cornea scarring in rabbit cornea keratoplasty.
研究领域:
生物医学工程
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材料科学
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组织工程
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再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2024
·
IF 6.1
Yang Liu,Xiaoting Chen,Xueqin Tan,Yeqian Huang,Wen Zhang,Zhicun Wang,Li Yang,Yunbing Wang,Zhengyong Li,Xingdong Zhang
Microtia profoundly affects patients' appearance and psychological well-being. Tissue engineering ear cartilage scaffolds have emerged as the most promising solution for ear reconstruction. However, constructing tissue engineering ear cartilage scaffolds requires multiple passaging of chondrocytes, resulting in their dedifferentiation and lose their special phenotypes and functions. To tackle these issues, here we employ guanidinobenzoic acid (GBA) modified Generation 5 Polyamidoamine (PAMAM) dendrimers (PG) as Runx1 plasmid carrier to construct PG/pRunx1 polyplex nanoparticles. The PG/pRunx1 polyplexes are transfected into human auricular chondrocytes, significantly mitigating chondrocyte dedifferentiation and enhancing cartilage regeneration during the in vitro culture. Furthermore, we develop a highly porous double-network hydrogels based on methacrylate-functionalized and oxidized chondroitin sulfate and carbohydrazide-modified gelatin and the hydrogels possessed both dynamic adaptability and mechanical support characteristics by reversible dynamic covalent crosslinking and static covalent crosslinking, serving as an ideal scaffold for tissue engineering. Consequently, chondrocytes treated with PG/pRunx1 polyplex nanoparticles are incorporated into the hydrogels to construct tissue-engineered auricular cartilage scaffolds. After subcutaneous implantation in nude mice, the scaffolds containing chondrocytes treated with PG/pRunx1 nanoparticles showed more mature cartilaginous tissue, characterized by prominent ECM deposition and enhanced chondrogenesis. Therefore, this research provides a novel strategy for the development of tissue-engineered auricular cartilage scaffolds.
研究领域:
组织工程
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再生医学
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生物材料
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生物医学工程
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）