Journal of Analysis and Testing
·
2023
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IF 4.7
Gong Xue,Wu Jiangling,Zhang Jiajia,Jiang Zhongwei,Wang Yi,Zhang Pu
Heart failure (HF) is a clinical hallmark of cardiovascular syndrome leading to substantial morbidity, repeated hospitalization, and enormous healthcare expenditure. As elderly populations continue to increase, biomarker-guided diagnosis and treatment for age-related cardiac decline have become essential. Accumulating evidence demonstrates that N-terminal proBNP (NT-proBNP) can provide a unique window into the diagnosis and risk stratification with HF. Herein, a simple yet robust aptasensor is developed for on-site recognizing the NT-proBNP by its targeting aptamer, thus achieving the accurate diagnosis of HF. This aptasensing system is prepared by absorbing the fluorophore-labeled aptamer strand onto the graphene oxide (GO), leading to efficient quenching without possible off-site signal leakage. The aptamer strand can specifically identify target NT-proBNP molecules via a versatile conformational transformation, resulting in the desorption of the aptamer-NT-proBNP complexes from GO and re-generation fluorescence signal, thus allowing sensitive detection of NT-proBNP in 37 clinical blood samples. Taking advantage of the high specificity of aptamer-guaranteed recognitions of NT-proBNP, this aptasensor system readily achieves better diagnostic performance for HF than commercially adopted chemiluminescence immunoassay (Siemens, CLIA) in hospitals in terms of accuracy (89.2% vs 83.8%), specificity (89.5% vs 84.2%), and positive predictive value (88.9% vs 83.3%). This work provides a stable option for the diagnosis and treatment of elderly HF-related diseases in clinics.
研究领域:
医学
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生物技术
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Jian Sun,Pei He,Rui Wang,Zhong-Yuan Zhang,Yu-Qing Dai,Xiao-Yu Li,Si-Yu Duan,Cai-Ping Liu,Hao Hu,Guang-Jun Wang,Yan-Ping Zhang,Fei Xu,Rui Zhang,Yi Zhao,Hui-Fang Yang
Experimental evidence has demonstrated that neonicotinoids (NEOs) exposure can cause lipid accumulation and increased leptin levels. However, the relationship between NEOs exposure and dyslipidemia in humans remains unclear, and the interactive effects of NEOs and their characteristic metabolites on dyslipidemia remain unknown. We detected 14 NEOs and their metabolites in urine samples of 500 individuals (236 and 264 with and without dyslipidemia, respectively) randomly selected from the baseline of the Yinchuan community-dwelling elderly cohort (Ningxia, China). The NEOs and their metabolites were widely detected in urine (87.2–99.6 %) samples, and the median levels ranged within 0.06–0.55 μg/g creatinine. The positive associations and dose-dependent relationships of thiacloprid , imidacloprid-olefin, and imidacloprid-equivalent total with dyslipidemia were validated using restricted cubic spline analysis. Mixture models revealed a positive association between the NEOs mixture and dyslipidemia risk, with urine desnitro-imidacloprid ranked as the top contributor. The Bayesian Kernel Machine Regression models showed that the NEOs mixtures were associated with increased dyslipidemia when the chemical mixtures were ≥ 25th percentile compared to their medians, and desnitro-imidacloprid and imidacloprid-olefin were the major contributors to the combined effect. Given the widespread use of NEOs and the dyslipidemia pandemic, further investigations are urgently needed to confirm our findings and elucidate the underlying mechanisms.
研究领域:
公共卫生
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环境医学
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老年医学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Ya-Fang Shang,Jun-Hao Miao,Jia Zeng,Tian-Hua Zhang,Rui-Ming Zhang,Bing-Yan Zhang,Chao Wang,Yi-Long Ma,Xiang-Li Niu,Xiao-Long Ni,Zhao-Jun Wei
We evaluated the in vitro digestibility of apple polyphenols mimicking elderly and adult digestion models (dynamic and static systems). The digestibility of total apple polyphenols in small intestine was much higher in the adult dynamic system (62 μg/100 g fresh apple) compared to the static system (20 μg/100 g fresh apple) and elderly dynamic digestion conditions (33 μg/100 g fresh apple). Elderly in vitro static digestion showed better antioxidant activity than the adult system ( OH and ABTS + methods). Thus, the in vitro dynamic digestion system can more truly reflect the digestion of apple polyphenols than static digestion system. Moreover, elderly digestion conditions negatively influenced the digestibility of apple polyphenols including chlorogenic acid , epicatechin , phlorizin , rutin , phloretin , hyperoside , proanthocyanidin B 2 , and quercetin . Hence, appropriate selection of in vitro digestion models for elderly is a prerequisite to exploring the digestibility of phytochemicals for the development of functional food products for elderly.
研究领域:
食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CLINICAL LABORATORY ANALYSIS
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2022
·Vol. 36
·期 4
·e24350
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IF 3.124
Fenghui Pan,Wenxia Cui,Lei Gao,Xiaoting Shi,Haiyan Yang,Yun Hu,Man Li
Objective To investigate the relationship between serum lutein and type 2 diabetes mellitus (T2DM) and diabetic kidney disease (DKD) in elderly individuals. Methods A total of 60 T2DM patients over 60 years were subgrouped into a DKD group and a non-DKD group according to their urinary microalbumin-to-creatinine ratio (UACR), while 30 age-matched non-T2DM patients were recruited in the control group. Baseline characteristics, laboratory examination results, and serum lutein levels were compared, and their correlations were analyzed. Receiver operating characteristic (ROC) curves were plotted to identify the diagnostic potential of lutein in T2DM and DKD. Results The lutein level in the T2DM group was significantly lower than that in the control group and was also significantly lower in the DKD group than in the non-DKD group ( p < 0.001). Lutein levels were negatively correlated with body mass index, glycosylated hemoglobin, fasting blood glucose, triglyceride, and UACR and positively correlated with high-density lipoprotein cholesterol ( p < 0.05). T2DM patients were divided into four groups according to the quartile of their lutein level. The proportion of T2DM and DKD gradually decreased with increasing lutein levels ( p < 0.001). The area under the ROC curve of serum lutein in diagnosing T2DM and DKD was 0.880 and 0.779, respectively, with corresponding cut-off values of 0.433 μmol/L and 0.197 μmol/L ( p < 0.001). Conclusion The serum level of lutein is negatively correlated with the incidence of T2DM and DKD in the elderly and can serve as a diagnostic marker for T2DM and DKD.
研究领域:
医学
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内分泌学
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老年医学
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DOI
PubMed
高影响因子
CHEMICAL PAPERS
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2024
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IF 2.1
Liu Zitong,Liu Jiali
Green water-based paint cannot exhibit antibacterial property. For good antibacterial property, ZnO/reduced graphene oxide (rGO) nanofiller was added. Graphene oxide and ZnO/rGO composite were synthesized. Then, ZnO/rGO/paint composite coating with excellent antibacterial property was prepared. As ZnO content in ZnO/rGO filler is increased, antibacterial property of coating is improved. In fighting against Escherichia coli and Staphylococcus aureus , pristine coating shows low antibacterial rates of 0.50% and 0.48%, respectively. When ZnO was added, antibacterial rates of coating were be increased to 49.31% and 41.67%. When ZnO/rGO filler was used, antibacterial rates were increased to 65.46% and 62.17%. At a higher load of ZnO/rGO filler, antibacterial rates reached 93.37% and 83.38%. Friction coefficient of ZnO/rGO/paint composite coating was decreased to 0.53 compared with original coating. At a higher dose of ZnO/rGO filler, friction coefficient was decreased to 0.40. Properties of ZnO/rGO/paint coating meet a national standard for water-based wood coatings for indoor decoration. This composite coating with excellent antibacterial property and wear resistance can be applied in eco-friendly indoor decoration.
研究领域:
材料科学
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环境科学
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微生物学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Jiaxin Chang,Yan Zeng,Huangqiao Tao,Dejun Hu,Wei Zhao,Suilou Wang,Chongjiang Cao
Polysaccharide hydrocolloids are widely used in milk protein to improve swallowing safety in dysphagia, but their effects on gastric digestion require critical investigation. This study compared xanthan gum (XG; with excellent rheological properties but potential digestion-impairing effects) and Naematelia aurantialba polysaccharide (NAP; a potential thickening polysaccharide) for their effects on sodium caseinate (SC) and whey protein (WP) digestibility and gastric emptying. The experiments utilized both INFOGEST (static) and DHS-IV (dynamic) digestion models under simulated elderly conditions. Results demonstrated that the addition of XG hinders protein digestion and delays gastric emptying. Specifically, XG reduced free amino groups release by 20.26 ± 0.08 % (SC-XG) and 21.82 ± 0.04 % (WP-XG). The gastric half-emptying time was prolonged by 14.90 ± 1.07 min (SC-XG) and 15.26 ± 1.10 min (WP-XG). In contrast, NAP was found to promote gastric-phase protein digestion. This study provides novel insights for the design of dysphagia-friendly foods for elderly populations.
研究领域:
食品科学
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营养学
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生物技术
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老年医学
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高分子科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Electronic Materials
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2025
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IF 4.7
Yunlu Wang,Mengqi Zhang,Zihang Cheng,Qingyang Feng,Zida An,Dongsheng Liu,Wenxu Ni,Youshan Ma,Zhe Sun,Yupeng Mao
Accelerated aging of the population has led to an increase in the incidence of orthopedic chronic diseases and acute illness, which has placed a significant burden on the public health and economy. Continuous physiological monitoring and timely rehabilitation interventions represent crucial strategies for early disease detection, functional independence preservation, and healthcare cost containment among elderly individuals. This study proposes a single-electrode triboelectric nanogenerator (PE-TENG) for elderly assistance systems, developed through the integration of a supramolecular conductive hydrogel (PCBC-hydrogel) with a soft Ecoflex layer. The PCBC-hydrogel, engineered from poly(vinyl alcohol) (PVA), chitosan, borate cross-linking, and carbon nanotubes (CNTs), serves as an advanced friction electrode material that combines mechanical compliance with stable electrical performance, making it particularly suitable for aging-care applications. The pristine PVA hydrogel showed moderate mechanical properties. Boric acid formed dynamic borate bonds with PVA hydroxyls, enhancing the elasticity and strength. Material characterization reveals that dynamic borate ester bonding between boric acid and PVA hydroxyl groups enhances mechanical resilience, achieving a tensile strength of ∼209 kPa. The introduction of chitosan facilitates additional hydrogen bonding networks, enabling rapid self-recovery within 10 s. Incorporated CNTs provide stable electrical conductivity (0.13 S/m) while reinforcing the hydrogel matrix. The PE-TENG demonstrates reliable operation with a 90 s response time and maintains a consistent performance through 3000 cycles. This design allows for real-time monitoring of physiological signals through mechanoelectrical transduction. The utilization of supramolecular hydrogel TENG-based health monitoring systems, smart home assistance systems, and bionic effector systems enables the real-time monitoring of the physical condition of the elderly, facilitating the promotion of their healthy lifestyles through the monitoring of the home environment and bionic control. This work not only promotes the development of hydrogel-based TENGs and provides another direction for self-powered wearable devices but also establishes a foundation for future smart aging-care systems.
研究领域:
生物医学工程
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材料科学
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老年医学
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产品关联
DOI
高影响因子
Food Bioscience
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2023
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IF 5.2
Yinlong Ma,Jinzhao Xu,Ruibin Guo,Guoxin Teng,Yidan Chen,Xiaoxi Xu
With the increase in global life expectancy, the research on the specific digestive patterns of the elderly is becoming highly essential. We simulated the digestion process of older individuals in vitro and investigated the effects of various high hydrostatic pressure (HHP) treatments on the protein structure, digestibility , and antioxidant activity of whey protein isolate (WPI), as well as the antioxidant peptides produced from its digest. The HHP treatment specifically affected on the secondary and tertiary protein structures along with the free sulfhydryl content of WPI. Protein microstructure revealed a significant increase in the number and depth of pores. Highest in vitro digestibility and cellular antioxidant activity was observed after HHP treatment at 600 MPa for 30 min. In the intestinal digest, 15 antioxidant peptides were identified whose characteristics matched those of bioactive peptides . Of these, the LGSDMEDLR peptide sequence had the highest "-CDOCKER Energy” and formed the salt bridge, attractive charge, conventional hydrogen bond , carbon hydrogen bond, and van der Waals with Arg380, Arg415, Arg483, Tyr334 and Asn382, which all located in the binding site of the Keap1-Nrf2 interaction. Overall, HHP appeared to be a potential method for the application of dairy products among the elderly.
研究领域:
食品科学
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营养学
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老年学
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生物化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Jingjie Hou,Guixin Tan,Afeng Wei,Shan Gao,Huajiang Zhang,Wentao Zhang,Yujia Liu,Rui Zhao,Yunze Ma
In this study, a carboxymethylcellulose (CMC) induced depletion attraction was developed to stabilize high internal phase Pickering emulsions (HIPPEs) as age-friendly 3D printing inks. The results demonstrated that depletion force induced the adsorption of yolk particles at the droplet interface and the formation of osmotic droplet clusters, thereby increasing the stability of HIPPEs. In addition, the rheological properties and nutrient delivery properties of HIPPEs could be adjusted by the mass ratio of yolk/CMC. The HIPPEs stabilized at yolk/CMC mass ratio 20:7.5 showed optimal printability, viscoelastic, structural recovery, and swallowability. HIPPEs have been applied to 3D printing, International Dysphagia Dietary Standardization Initiative (IDDSI) test, and in vitro digestive simulation in the elderly, indicating their attractive appearance, safe swallowability, and enhanced bioaccessibility of β-carotene. Our work provides new ideas for developing age-friendly foods with plasticity and nutrient delivery capacity by depletion attraction stabilizing HIPPEs.
研究领域:
食品科学
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材料科学
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老年医学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Aggregate
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2024
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IF 13.9
Yuanting Xie,Kaidi Li,Jing Liu,Ying Zhou,Chuangjie Zhang,Yan Yu,Jianlong Wang,Lei Su,Xueji Zhang
Frequent nocturia-induced nighttime visits aggravate falls in seniors, requiring synthetic antidiuretic drugs that risk the dangerous syndrome of inappropriate antidiuretic hormone secretion (SIADH), thus the detection of sodium ion and uric acid alterations during treatment is obligatory for drug-safe management. Herein, we design a convolutional neural network (CNN)-enhanced smart wearable microneedle array-based colorimetric (WMNC) sensor to independently detect in vivo interstitial fluid (ISF) sodium ions and uric acid alterations. The WMNC sensor is composed of a vacuum tube-driven microneedle array patch and a built-in colorimetric sensing paper, enabling an efficient ISF extraction and rapid colorimetric assay. Furthermore, leveraging self-designed CNNs, the WMNC sensor efficiently eliminates the influence of ambient light on colorimetric outcomes, facilitating a rapid and accurate colorimetric result classification. This study provides an ISF-based rapid, intuitionistic, user-friendly, wearable point-of-care technique for the elderly suffering from nocturia in monitoring their health status for early warnings of SIADH.
研究领域:
生物医学工程
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可穿戴技术
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即时诊断
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神经网络
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健康监测
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Cardiovascular Medicine
·
2024
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IF 2.8
Chen Hui-Lian,Wang Qing-Yu,Qi Ruo-Mei,Cai Jian-Ping
Background: Platelet hyperreactivity is a risk factor for thrombosis in elderly patients with cardiovascular diseases. However, the mechanism of platelet hyperactivation has not been elucidated. This study aims to investigate alterations in the proteomes of platelets and their correlation with platelet hyperreactivity among elderly individuals.Methods: This study included 10 young (28.1 ± 1.9 years), 10 middle-aged (60.4 ± 2.2 years), and 10 old (74.2 ± 3.0 years) subjects. Washed platelets were used in the present study. Platelet samples were analysed by using data-independent acquisition (DIA) quantitative mass spectrometry (MS).Results: The results showed that the platelet proteomic profile exhibited high similarity between the young and middle-aged groups. However, there were significant differences in protein expression profiles between the old group and the young group. By exploring the dynamic changes in the platelet proteome with ageing, clusters of proteins that changed significantly with ageing were selected for further investigation. These clusters were related to the initial triggering of complement, phagosome and haemostasis based on enrichment analysis. We found that platelet degranulation was the major characteristic of the differentially expressed proteins between the old and young populations. Moreover, complement activation, the calcium signalling pathway and the nuclear factor-κB (NF-κB) signalling pathway were enriched in differentially expressed proteins.Conclusions: The present study showed that there are obvious differences in the protein profiles of the elderly compared with young and middle-aged populations. The results provide novel evidence showing changes in platelet hyperactivity and susceptibility to thrombosis in the elderly population.
研究领域:
血液学
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蛋白质组学
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衰老研究
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心血管疾病
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2023
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IF 8.1
Jingjie Hou,Guixin Tan,Shihui Hua,Huajiang Zhang,Jing Wang,Ning Xia,Sijie Zhou,Dong An
The work aimed to develop the multi-protein mixture of egg yolk as natural particles to stabilize high internal phase Pickering emulsions (HIPPEs) to improve the bioaccessibility of β-carotene in the elderly. The results showed that the depletion attraction drove the adsorption of egg yolk protein particles at the oil–water interface and the formation of osmotic droplet clusters due to the attachment of particle-coated droplets in the dispersed phase, leading to kinetic blocking and stable gelation of HIPPEs. Rheological measurements showed that HIPPEs had shear thinning, low shear stress, viscoelastic properties, and structural recovery properties, which facilitated easy consumption for the elderly. The stability of HIPPEs was verified by ionic and centrifugal stability tests, demonstrating their potential for application to complex gastric environments. HIPPEs have been applied to the International Dysphagia Dietary Standardization Initiative (IDDSI) test and simulated in vitro digestion in older adults, demonstrating their safe swallowability and high β-carotene bioaccessibility. Our findings suggest solutions for food practitioners facing the aging problem and provide new insights for preparing age-friendly foods.
研究领域:
食品科学
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营养学
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老年学
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胶体科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）