AQUATIC TOXICOLOGY
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2019
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IF 3.794
Jian Han,Kechun Liu,Rongchun Wang,Yun Zhang,Bingsheng Zhou
Otolith consisting largely of calcium carbonate, fibrous and proteins, is vital for maintaining body balance and/or hearing of fish. The formation of otolith involves Ca 2+ transport and deposition. In the present study, we investigated the effects of Cd 2+ on otoliths development by using zebrafish embryos as model. The results showed that exposure to Cd 2+ inhibited the utricular and saccular otoliths growth, indicated by reduced lateral areas. Swimming speeds were reduced and a losing balance control was observed in Cd 2+ exposed larvae. The genes related to Ca 2+ transport (e.g. plasma membrane Ca 2+ -ATPase isoform 2 , pmca2 ; Ca 2+ -ATPase isoform 2 , atp2b1a ) and regulation (e.g. parathyroid hormone ligand type-1 , pth1 ; stanniocalcin isoform 1 , stc1 ) were significantly downregulated. However, the adverse effects of Cd 2+ on otoliths growth and swimming activity can be protected by supplementation of Ca 2+ in exposure medium. Body burden of Cd 2+ in larvae was reduced upon the supplement with Ca 2+ . The overall results suggest that exposure to Cd 2+ can inhibit influx of Ca 2+ , leading to less deposition of CaCO 3 for otolith growth, and finally result in impaired balance control and swimming activity in zebrafish larvae.
研究领域:
环境毒理学
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发育生物学
✓ 已验证
DOI
PubMed
高影响因子
Materials
·
2023
·Vol. 16
·期 21
·6900
·
IF 3.4
Qi Wang,Minghao Fang,Xin Min,Pengpeng Du,Zhaohui Huang,Yangai Liu,Xiaowen Wu,Yulin Liu,Changmiao Liu,Feihui Huang
In recent years, regenerative thermal oxidizer (RTO) has been widely used in the petroleum industry, chemical industry, etc. The massive storage required by solid waste has become a serious problem. Due to their chemical composition, bauxite tailings as raw materials for high-temperature thermal storage ceramics show enormous potential in the fields of research and application. In this study, we propose a method for preparing ferric-rich and high specific storage capacity by adding Fe2O3powder to bauxite tailings. Based on a 7:3 mass ratio of bauxite tailings to lepidolite, Fe2O3powder with different mass fractions (7 wt%, 15 wt%, 20 wt%, 30 wt%, and 40 wt%) was added to the ceramic material to improve the physical properties and thermal storage capacity of thermal storage ceramics. The results showed that ferric-rich thermal storage ceramics with optimal performance were obtained by holding them at a sintering temperature of 1000 °C for 2 h. When the Fe2O3content was 15 wt%, the bulk density of the thermal storage ceramic reached 2.53 g/cm3, the compressive strength was 120.81 MPa, and the specific heat capacity was 1.06 J/(g·K). This study has practical guidance significance in the preparation of high thermal storage ceramics at low temperatures and low costs.Keywords:bauxite tailings;thermal storage ceramic;specific heat capacity;bulk density;RTO
研究领域:
材料科学
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环境工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Cell and Developmental Biology
·
2020
·
IF 5.186
Zhang Yan,Wang Yanfei,Yao Xuebo,Wang Changquan,Chen Fangyi,Liu Dong,Shao Ming,Xu Zhigang
Hair cells in the inner ear and lateral lines are mechanosensitive receptor cells whose development and function are tightly regulated. Several transcription factors as well as splicing factors have been identified to play important roles in hair cell development, whereas the role of RNA stability in this process is poorly understood. In the present work, we report that RNA-binding motif protein 24a (Rbm24a) is indispensable for hair cell development in zebrafish. Rbm24a expression is detected in the inner ear as well as lateral line neuromasts. Albeit rbm24a deficient zebrafish do not survive beyond 9 days post fertilization (dpf) due to effects outside of the inner ear, rbm24a deficiency does not affect the early development of inner ear except for delayed otolith formation and semicircular canal fusion. However, hair cell development is severely affected and hair bundle is disorganized in rbm24a mutants. As a result, the auditory and vestibular function of rbm24a mutants are compromised. RNAseq analyses identified several Rbm24a-target mRNAs that are directly bound by Rbm24a and are dysregulated in rbm24a mutants. Among the identified Rbm24a-target genes, lrrc23, dfna5b, and smpx are particularly interesting as their dysregulation might contribute to the inner ear phenotypes in rbm24a mutants. In conclusion, our data suggest that Rbm24a affects hair cell development in zebrafish through regulating mRNA stability.
研究领域:
发育生物学
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分子生物学
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神经科学
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遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
·
2015
·
IF 4.046
Jie Ding,Zihua Tang,Jiarong Chen,Haosong Shi,Jianling Chen,Cuicui Wang,Cui Zhang,Liang Li,Ping Chen,Jinfu Wang
Sensorineural hearing loss and vestibular dysfunction have become the most common forms of sensory defects. Stem cell-based therapeutic strategies for curing hearing loss are being developed. Several attempts to develop hair cells by using chicken utricle stromal cells as feeder cells have resulted in phenotypic conversion of stem cells into inner ear hair-cell-like cells. Here, we induced the differentiation of human embryonic stem cells (hESCs) into otic epithelial progenitors (OEPs), and further induced the differentiation of OEPs into hair-cell-like cells using different substrates. Our results showed that OEPs cultured on the chicken utricle stromal cells with the induction medium could differentiate into hair-cell-like cells with stereociliary bundles. Co-culture with stromal cells, however, may be problematic for subsequent examination of the induced hair-cell-like cells. In order to avoid the interference from stromal cells, we cultured OEPs on laminin with different induction media and examined the effects of the induction medium on the differentiation potentials of OEPs into hair-cell-like cells. The results revealed that the culture of OEPs on laminin with the conditioned medium from chicken utricle stromal cells supplemented with EGF and all-trans retinoic acid (RA) could promote the organization of cells into epithelial clusters displaying hair-cell-like cells with stereociliary bundles. These cells also displayed the expected electrophysiological properties.
研究领域:
干细胞生物学
·
耳鼻喉科学
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再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）