ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2022
·Vol. 415
·期 18
·4265-4275
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IF 4.478
Wang Tingting,Chen Yihui,Hou Chunyan,Qiao Xiaoqiang
A novel type of PEG-modified halloysite was prepared and used as a hydrophilic interaction and cation exchange mixed-mode sorbent for solid-phase extraction of biogenic amines in fish samples. The eluates were analyzed by high-performance liquid chromatography-ultraviolet detection after the derivatization with benzoyl chloride. The developed sorbent was characterized by scanning electron microscopy, infrared spectroscopy, X-ray diffraction, zeta potential analyzer, and thermo-gravimetric analysis. After the optimization of various parameters influencing the extraction efficiency, the PEG-modified halloysite-based SPE method was evaluated. The adsorption capacities of putrescine, spermine, phenethylamine, and histamine were as high as 9.3, 8.5, 5.7, and 5.6 mg g −1 , respectively. Satisfactory reproducibility of sorbent preparation was obtained with within-batch and batch-to-batch relative standard deviations (RSDs) lower than 3.9% and 8.6%, respectively. The biogenic amine spiking recoveries in fish samples ranged from 84.3 to 105.5% with good RSDs lower than 7.8%. Intra-day and inter-day precision, expressed as RSDs, were better than 8.8%. The limits of detection of histamine, putrescine, phenethylamine, and spermine were 9.4, 1.9, 0.5, and 0.9 μg L −1 , respectively. This work provides a new hydrophilic interaction and cation exchange mixed-mode sorbent and is successfully applied to the extraction of trace biogenic amines from fish samples. Graphical Abstract
研究领域:
分析化学
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食品科学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2023
·
IF 5.8
Jian Chen,Hui Wang,Li Zhang,Wanying Yan,Ruilong Sheng
Developing low-cost, easy-to-prepare, biocompatible and highly efficient vaccine carriers is a promising approach to realize practical cancer immunotherapy . In this study, through facile modification of mPEG5k-4-toluenesulfonate (mPEG5k-OTs) on PEI25k under mild conditions, a series of “stealth” mPEG5k-PEI25k polymers (PP1, PP2 and PP3) were prepared, their structures and physicochemical properties were characterized and theoretically analyzed. The polymers could bind/load ovalbumin (OVA) to form mPEG5k-PEI25k/OVA complexes as negatively charged nanoparticles with small hydrodynamic particle size (80–210 nm) and narrow size distribution. Compared to PEI25k/OVA, lower cytotoxicity could be achieved on mPEG5k-PEI25k/OVA complexes in dendritic cells (DCs). In DCs-RF 33.70 T-cells co-culture system, the mPEG5k-PEI25k/OVA complexes could bring about higher IL-2 production /secretion than that of PEI25k/OVA, notably, the optimum IL-2 secretion could reach 9.3-folds of the PEI25k/OVA under serum condition (10% FBS). Moreover, the cell biological features could be optimized by selecting suitable mPEG5k-grafting ratios and/or mPEG5k-PEI25k/OVA weight ratios. Intracellular imaging results showed that the mPEG5k-PEI25k(PP3)/Rhodamine-OVA complexes mainly localized inside lysosomes . Taken together, this work provided a facile method to prepare “stealth” PEGylated-PEI25k polymers with reduced cytotoxicity, promoted OVA cross-presentation efficiency and improved serum compatibility towards cancer immunotherapy.
研究领域:
生物医学工程
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免疫学
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DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·
IF 10.6
Zifei Wu,Xie Huang,Chuan Wu,Yan Zhou,Mingquan Gao,Shenglin Luo,Qiang Xiang,Weidong Wang,Rong Li
Enhancing the sensitivity of radiotherapy (RT) towards renal cell carcinoma (RCC) remains a challenge because RCC is a radioresistant tumor. In this work, we design and report asymmetrically Polyethylene Glycol (PEG)ylated and amphipathic heptamethine indocyanine dyes for efficient radiosensitization of RCC treatment. They were synthesized by modifying different lengths of PEG chains as hydrophilic moieties on one N -alkyl chain of a mitochondria-targeting heptamethine indocyanine dye (IR-808), and a radiosensitizer 2-nitroimidazole (NM) as a hydrophobic moiety on another N -alkyl chain. The PEG modification significantly improved water solubility, decreased the intermolecular π–π large aggregates, thereby enhanced renal excretion. The asymmetrical and amphipathic modification enhanced the preferential accumulation in renal tumors through self-assembly into small-size nanoparticles in aqueous environment. Radiosensitization was further improved by preferential accumulation in renal tumor cells and their mitochondria as mitochondria play a crucial role in rapid cancer cell growth, metastasis, and RT resistance. Additionally, the modification also increased the abilities of fluorescence emission and photostability, which is meaningful for imaging-guided precise RCC RT. Therefore, our findings may present a theranostic radiosensitizer for renal tumor-targeted imaging and radiosensitization.
研究领域:
肿瘤学
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药理学
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2021
·Vol. 109
·期 9
·1303-1312
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IF 3.368
Ji Ji,Guang Chen,Zitong Liu,Lili Li,Jiugang Yuan,Ping Wang,Bo Xu,Xuerong Fan
To improve mechanical properties of keratin (KR) porous scaffolds, we prepared a PEGylated keratin through thiol-ene click reaction. Several porous scaffolds were prepared by blending PEGylated keratin with sodium alginate (SA). The surface morphology, mechanical properties, and porosity of scaffolds were detailed studied at different KR/SA proportions. The results showed the content of SA had an effect on pore formation and mechanical properties. When the mass ratio of KR to SA was 2:1, the stress of yield point of the keratin porous scaffold reached 1.24 MPa, and also showed good deformation recovery ability. The PEGylated keratin porous scaffold had a high porosity and great cytocompatibility. Its' porosity is up to 81.7% and the cell viability is about 117.78%. This allows it to absorb the simulated plasma quickly (9.20 ± 0.37 g/g). In addition, the structural stability and acid–base stability of the keratin porous scaffold were also improved after PEGylation. Overall, the PEGylated keratin porous scaffold will be promising in tissue materials due to its great physical, chemical, and biological properties.
研究领域:
材料科学
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生物医学工程
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DOI
PubMed
高影响因子
LANGMUIR
·
2020
·
IF 3.557
Junnan Yang,Wei Liu,Yan Sun,Xiaoyan Dong
Deposition of amyloid-β (Aβ) aggregates in the brain is a main pathological hallmark of Alzheimer’s disease (AD), so inhibition of Aβ aggregation has been considered as a promising strategy for AD prevention and treatment. Black phosphorus (BP) is a 2D nanomaterial with high biocompatibility and unique biodegradability, but its potential application in biomedicine suffers from the rapid degradability and unfunctionability. To overcome the drawbacks and broaden its application, we have herein designed an Aβ inhibitor (LK7)-coupled and polyethylene glycol (PEG)-stabilized BP-based nanosystem. The PEGylated-LK7-BP nanosheets (PEG-LK7@BP) not only exhibited a good stability but also demonstrated a significantly enhanced inhibitory potency on Aβ42 fibrillogenesis in comparison with its counterparts. This elaborately designed PEG-LK7@BP stopped the conformational transition and suppressed the fibrillization of Aβ42, so it could completely rescue cultured cells from the toxicity of Aβ42 (by increasing the cell viability from 72 to 100%) at 100 μg/mL. It is considered that PEG-LK7@BP could bind Aβ species by enhanced electrostatic and hydrophobic interactions and thus efficiently alleviated Aβ–Aβ interactions. Meanwhile, the coupled LK7 on the BP surface formed a high local concentration that enhanced the affinity between the nanosystem and Aβ species. Finally, PEG could improve the stability and dispersibility of the nanoplatform to make it show an increased inhibitory effect on the amyloid formation. Hence, this work proved that PEG-LK7@BP is a promising nanosystem for the development of amyloid inhibitors fighting against AD.
研究领域:
生物医学
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纳米材料科学
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神经退行性疾病研究
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 32
·25329-25336
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IF 3.84
Qing Wan,Jianwen Tian,Meiying Liu,Guangjian Zeng,Zhen Li,Ke Wang,Qingsong Zhang,Fengjie Deng,Xiaoyong Zhang,Yen Wei
The biomedical applications of carbon nanotubes (CNTs) have been intensively investigated. However, poor water dispersibility and obvious toxicity of pristine CNTs are still two major issues for their biomedical applications. Although great efforts have been devoted to solving these problems, a simple and effective strategy for preparation of CNTs with high water dispersibility and desirable biocompatibility is still of great research interest. Herein, surface modification of CNTs with a biocompatible polymer polyethylene glycol (PEG) via a mussel inspired strategy has been developed. The dispersibility as well as biocompatibility of these PEGylated CNTs (named as CNT-poly(PEGMA-co-IA-DA)) was subsequently investigated. These PEGylated CNTs showed remarkable enhancement of dispersibility in aqueous and organic solvents. More importantly, as evidenced by cell viability and reactive oxygen species results, these PEGylated CNTs showed negative toxicity toward cancer cells. Therefore the PEGylated strategy described in this work can provide a general platform for fabrication of multifunctional biomaterials for various biomedical applications because of the advantages of mussel inspired chemistry and the excellent properties of PEGylated materials.
研究领域:
材料科学
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生物医学工程
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纳米技术
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DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2015
·Vol. 132
·期 31
·
IF 1.768
Haitao Huang,Tao Yue,Yanyun Xu,Ke Xu,Haibo Xu,Shiyuan Liu,Jiahui Yu,Jin Huang
To enhance biocompatibility and physiological stability of hydrophobic MnO nanoparticles as contrast agent of T 1 -weighted magnetic resonance imaging (MRI), dopamine-functionalized poly(ethylene glycol) (PEG) was used to coat the surface of about 5 nm MnO nanoparticles. Although hydrophilic coating might decrease longitudinal relaxivity due to inhibiting the intimate contact between manganese of nanoparticle surface and proton in water molecules, higher longitudinal relaxivity was still maintained by manipulating the PEGylation degree of MnO nanoparticles. Moreover, in vivo MRI demonstrated considerable signal enhancement in liver and kidney using PEGylated MnO nanoparticles. Interestedly, the PEGylation induced the formation of about 120 nm clusters with high stability in storing and physiological conditions, indicating passive targeting potential to tumor and prolonged circulation in blood. In addition, the cytotoxicity of PEGylated MnO nanoparticles also proved negligible. Consequently, the convenient PEGylation strategy toward MnO nanoparticles could not only realize a good “trade-off” between hydrophilic modification and high longitudinal relaxivity but also contribute additional advantages, such as passive targeting to tumor and long blood circulation, to MRI diagnosis of tumor. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 42360.
研究领域:
材料科学
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医学影像学
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生物医学工程
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DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2017
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IF 3.234
Shengnan Li,Lingyu Zhang,Haipeng Zhang,Zhongcheng Mu,Lu Li,Chungang Wang
Elaborately designed novel multifunctional therapeutic agents are highly desired for efficient cancer therapy. In this work, a new therapeutic nanoplatform based on calcium phosphate/small gold nanorod assemblies modified with methoxy-poly(ethylene glycol)-thiol (designated as PEGylated CaP/Au NR assemblies) is created via a mild, reproducible, and simple route for the first time. The obtained PEGylated CaP/Au NR assemblies possess many virtues including outstanding drug-loading capacity, excellent photothermal conversion efficiency (η, ∼38.5%), pH/near-infrared (NIR) dual-responsive release property, and good biocompatibility. After loading doxorubicin (DOX) in PEGylated CaP/Au NR assemblies, the DOX-loaded PEGylated CaP/Au NR assemblies can simultaneously supply intense heating effect and increased DOX release under 808 nm NIR laser, achieving excellent antitumor therapeutic effect in vitro and in vivo. Furthermore, the combination of DOX-loading and photothermal treatment upon PEGylated CaP/Au NR assemblies displays better therapeutic effect than single chemotherapy or photothermal therapy. Furthermore, the comprehensive methyl thiazolyl tetrazolium (MTT), hemolysis, and histological assays manifest no obvious toxicity of PEGylated CaP/Au NR assemblies. Our work elucidates the great prospect of PEGylated CaP/Au NR assemblies as a therapeutic agent for synergistic chemo-photothermal cancer therapy.
研究领域:
生物医学工程
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纳米医学
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肿瘤学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Sha Jin,Jiaxun Wan,Lizheng Meng,Xiaoxing Huang,Jia Guo,Li Liu,Changchun Wang
The application of nanomaterials in intelligent drug delivery is developing rapidly for treatment of cancers. In this paper, we fabricated a new kind of protease/redox/pH stimuli-responsive biodegradable nanohydrogels with methacrylic acid (MAA) as the monomer and N,N-bis(acryloyl)cystamine (BACy) as the cross-linker through a facile reflux-precipitation polymerization. After that, the polyethylene glycol (PEG) and folic acid (FA) were covalently grafted onto the surface of the nanohydrogels for enhancement of their long in vivo circulation lifetime and active targeting ability to the tumor cells and tissues. This kind of nanohydrogels could be disassembled into short polymer chains (Mn < 1140; PDI < 1.35) both in response to glutathione (GSH) through reduction of the sensitive disulfide bonds and protease by breakage of the amido bonds in the cross-linked networks. The nanohydrogels were utilized to simultaneously load both hydrophilic drug doxorubicin (DOX) and hydrophobic drug paclitaxel (PTX) with high drug loading efficiency. The cumulative release profile showed that the drug release from the drug-loaded nanohydrogels was significantly expedited by weak acidic (pH 5.0) and reducing environment (GSH), which exhibited an distinct redox/pH dual stimuli-responsive drug release to reduce the leakage of drugs before they reach tumor site. In addition, the in vitro experiment results indicated that the multidrug-loaded system had synergistic effect on cancer therapy. Meanwhile, the acute toxicity and intravital fluorescence imaging studies were adopted to evaluate the biocompatibility and biotoxicity of the nanohydrogels, the experimental results showed that the PEG modification could greatly enhance the long in vivo circulation lifetime and reduce the acute toxicity (LD50: from 138.4 mg/kg to 499.7 mg/kg) of the nanohydrogels.
研究领域:
药学
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生物材料学
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肿瘤学
✓ 已验证
DOI
高影响因子
Journal of Water Process Engineering
·
2015
·
IF 0
Xiangrong Chen,Jianquan Luo,Benkun Qi,Weifeng Cao,Yinhua Wan
Polyethylene oxide (PEO)-modified ultrafiltration (UF) membranes were prepared using polyacrylonitrile-graft-polyethylene oxide (PAN-g-PEO) copolymers through immersion precipitation phase inversion method. Compared to PAN membrane, the PEO-modified UF membranes possessed extraordinary hydrophilic surface owing to the enrichment of PEO segments, which was confirmed by X-ray photoelectronic spectroscopy (XPS). The effects of membrane hydrophilicity on the organic fouling resistance were investigated by means of the resistance-in-series model. The results showed that the hydrophilic modification of UF membrane contributed to the fouling control in bovine serum albumin (BSA) and Escherichia coli ( E . coli ) bacteria filtration. The antifouling ability of the membranes increased with increasing PEO content. The pure water flux could recover completely after filtering BSA and bacteria, respectively, for the membranes with 25.2% and 8.5% PEO content. In contract, the increase of membrane hydrophilicity had a negative effect on the humic acid (HA) and sodium alginate (SA) filtration. The membrane permeability of the PAN membrane unexpectedly increased after HA or SA filtration, while the flux recovery ratio of PEO-modified membranes decreased with increasing PEO content. The differences in fouling behavior of the various organic foulants on hydrophobic or hydrophilic membrane were attributed to the nature of organic foulants and their interaction with membranes.
研究领域:
环境科学
·
生物技术
✓ 已验证
DOI
JOURNAL OF POLYMER SCIENCE
·
2025
·
IF 3.6
Yumiao Ma,Weijun Zhen,Zhe Wang,Tengfei Shao
In order to improve the crystallization properties and toughness of PLA film, poly(ethylene glycol) (PEG)-modified carbon dots (CDs) were synthesized as nanofillers to achieve this goal. PEG-CDs were characterized by Fourier transform infrared spectrometer (FTIR), static contact angle measurement, X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), and thermogravimetric analyzer (TGA), and the results demonstrated that the PEG chains were successfully grafted onto the surface of CDs. PEG-CDs/PLA composite films were prepared by extrusion blow molding technology, and their mechanical properties, thermal stability, anti-aging performance, and water vapor barrier properties were investigated. The results showed that the elongation at break of PEG-CDs/PLA composite films (0.05 wt%) increased from 19.43% (for pure PLA film) to 107.75%, and the water vapor barrier properties, thermal stability, and anti-aging properties of PEG-CDs/PLA composite films were improved to varying degrees compared with those of pure PLA film. The rheological behavior analysis of the PEG-CDs/PLA composite film revealed that PEG-CDs had a plasticizing effect on PLA, which is one possible cause for the increased toughness of the PEG-CDs/PLA composite films over pure PLA film. Moreover, the results of the crystallization behavior proved that PEG-CDs could not only improve the crystallinity of PLA but also accelerate its crystallization rate. Molecular dynamics simulations confirmed the presence of strong interactions between PEG-CDs and PLA, thereby enhancing the overall performance of PLA films. Graphical DSC and POM analyses indicate that PEG-CDs can effectively enhance the crystallinity of PLA. The results of isothermal and non-isothermal crystallization behavior show that PEG-CDs not only promote the crystallization of PLA but also accelerate its crystallization rate. Additionally, MD analysis demonstrates that there is a strong interaction between PEG-CDs and PLA. These factors collectively explain the effectiveness of PEG-CDs in improving the overall performance of PLA-based films.
研究领域:
材料科学
·
高分子化学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Xiaobin Zhang,Suling Zhang,Ting Lü,Wenfei Huang,Hongting Zhao,Hui Wang,Dong Zhang,Weixuan Yao
Anabolic steroids and β-agonists are commonly prohibited substances found in doping control studies; therefore, the determination of anabolic substances in biological samples is crucial. To analyze the anabolic compounds in urine, an adsorbent, polyethylene glycol (PEG)-grafted magnetic nanoparticle material (Fe 3 O 4 @SiO 2 -PEG), with low toxicity and strong biocompatibility was prepared in this investigation. Compared to those of Fe 3 O 4 and Fe 3 O 4 @SiO 2 , the grafted PEG chains (approximately 5.4 wt.%) on the magnetic nanoparticles improved the extraction efficiencies by factors of 3.9–17.0 and 2.5–2.9, respectively, likely due to the electrostatic attraction and hydrogen bonding. To achieve maximum extraction efficiency, several extraction parameters were optimized, including the kind and volume of desorption solvent, pH, and the extraction and desorption time. The standard curves were linear within the range of 0.5–20 μg/L for methyltestosterone and trenbolone, and 0.02–5 μg/L for clenbuterol. The limits of detection for the three drugs were 0.01–0.12 μg/L. The limits of quantification were 0.02–0.40 μg/L. The levels of precision of the optimized method were assessed based on the respective intra- and inter-day and batch-to-batch relative standard deviations in the ranges of 3.2–5.2 % ( n = 5), 5.9–11.3 % ( n = 4), and 6.7–9.2 % ( n = 3). The Fe 3 O 4 @SiO 2 -PEG nanoparticles could exclude urine matrix interferences (matrix effect of 91.8–98.1 %) and achieve satisfactory recoveries (75.5–116.1 %), affording sensitive and accurate determination of trace anabolic substances in urine.
研究领域:
分析化学
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药物分析
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）