RSC Advances
·
2016
·Vol. 6
·期 48
·42684-42692
·
IF 3.289
Jinglong Li,Fu Liu,Xuemin Yu,Ziyang Wu,Yunze Wang,Zhu Xiong,Jidong He
Surface heparinization is considered an efficient strategy to improve the hemocompatibility of polymeric membranes. We aim to realize the feasible surface heparinization of polylactide (PLA) membranes by means of a colourless 3-aminopropyltriethoxysilane (APTES) functionalized platform. APTES conjugated heparin (Hep–APTES) was first synthesized through the amidation reaction and then anchored onto PLA membrane via surface attachment, condensation, multilayer formation. The surface chemistry was confirmed by Fourier Transform Infrared-Attenuated Total Reflectance (ATR-FTIR) and X-ray Photoelectron Spectroscopy (XPS). The influences of heparinization time and micro-swelling agent (DMAc) on the heparin density, stability and sustained-release were investigated. The hydrophilicity, flux and morphology were also studied by contact angle, pure water flux and Scanning Electron Microscope (SEM). The hemocompatibility was evaluated by the activated partial thromboplastin time (APTT), prothrombin time (PT), the content of fibrinogen (FIB), platelet and Bovine Serum Albumin (BSA) adsorption respectively. All results demonstrated that APTES assisted surface heparinization provided a feasible and efficient strategy to improve the hemocompatibility of PLA membrane.
研究领域:
生物材料
·
医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Qingyan Chen,Kai Zhao,Guoping Li,Jiyue Luo,Xin Li,Yingjie Zeng,Xiao-mao Wang
Given the increasing usage of drinking water purifiers, highly permeable membranes with strong antimicrobial functions are desperately desirable for effective and durable water disinfection. Hereby, we prepared such antimicrobial membranes by chemical grafting of quaternary ammonium salt (QAS) molecules, 3-(trimethoxysilyl)propyldimethyloctadecyl ammonium chloride (TPMMC), onto air plasma pretreated biodegradable polylactic acid (PLA) substrates. The high chemical grafting density promoted very strong and positive zeta potential charge of the resulted PLA-QAS membrane, contributing to effective and broad-spectrum antimicrobial efficiencies (>99.99%) against different microbes, including fungi and conventional and drug-resistant bacteria. The solid grafting of QAS molecules produced a durable antimicrobial performance of the PLA-QAS membrane. In addition, the pleated filter (0.45 m2) of PLA-QAS membrane showed outstanding bacteria rejection properties (>99.99%) and excellent washing durability (up to 20 m3 water) even at very high water filtration rates (up to 4 L/min). The disinfection mechanism was clarified that negatively charged bacteria could be rapidly adsorbed to positively charged PLA-QAS spinnings, followed by devastating cell membrane damage to bacterial debris, leaving a clean environment without significant biofilm and biofouling formation.
研究领域:
材料科学
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环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2019
·
IF 7.015
Yang Liu,Qiu Han,Tiantain Li,Jing Hua,Fu Liu,Qiang Li,Gang Deng
Heparin free prescription is highly desired for haemodialysis. Versatile strategies have been developed to address the anti-coagulation modification of dialysis membranes. We here develop a facile and effective method to construct an anticoagulant coating on polymeric membranes or a dialyzer through hydrophobic-hydrophobic interaction. An amphiphilic heparin-like copolymer was first synthesized via a free radical polymerization based on alkyl chain lauryl methacrylate (LMA), acrylic acid (AA), and sodium 4-vinylbenze-sulfonate (SSNa) segments. The specific structure of terpolymer was characterized via Fourier transform infrared spectra (FTIR), Proton nuclear magnetic resonance ( 1 H NMR) spectra and size exclusion chromatography (SEC). The amphiphilic copolymer was anchored onto Polylactide (PLA) membrane surface from an aqueous-based solution via hydrophobic-hydrophobic interaction. An Attenuated total reflectance Fourier transform infrared spectra (ATR-FTIR), energy dispersive spectrometer (EDS) and X-ray photoelectron spectroscopy (XPS) confirmed the successful immobilization of heparin-mimicking terpolymers. The modified membranes showed excellent antifouling property and hydrophilicity. Moreover, the membranes exhibited outstanding haemocompatibility with suppressed platelet adhesion, low complement activation (C3a and C5a), expanded coagulation time and limited haemolysis ratio. The prolonged activated partial thromboplastin time and thrombin time (APTT>600 s, TT > 140 s) certified the excellent self-anticoagulant performances. To further examine the applicability and effectiveness, a polysulfone dialyzer was modified by this strategy. The ultrafiltration coefficient and clearance performances were examined. Overall, the hydrophobic-hydrophobic interaction strategy simplified the modification procedure of dialysis membrane, implying its great potential in heparin free dialysis application.
研究领域:
生物医学工程
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材料科学
·
血液净化技术
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yumiao Ma,Weijun Zhen,Tengfei Shao,Yikelamu Jilili,Qingqing Niu
Superhydrophilic separation membranes have broad application prospects in oil-water separation and wastewater purification. However, the accumulation of pollutants on the membrane surface and the secondary environmental pollution caused by waste membranes remain inevitable challenges. In this study, a superhydrophilic self-cleaning multifunctional membrane was fabricated by hydrolytic co-deposition of carbon dots, tetrabutyl titanate (TBT), and tannic acid on the surface of a degradable poly(lactic acid) (PLA) membrane for efficient separation of dye/oil/water emulsions. The results indicate that the superhydrophilic crosslinking network is formed on the surface of PLA-based membranes through co-deposition of TA-based coating, enabling the multifunctional membrane to possess a stable and ultra-strong oleophobic hydrophilic layer. As a result, the membrane exhibits strong underwater oil resistance and excellent performance in the separation of oil-in-water emulsions (rejection rate > 99%). More importantly, the surface of the superhydrophilic crosslinking network is negatively charged, which facilitates the selective removal of positively charged organic soluble substances in water through electrostatic adsorption. For instance, the removal rate of cationic dye MB and amphoteric dye RhB can reach as high as 99.93%. Additionally, with the catalysis of TiO 2 , the organic pollutants on the membrane surface can be decomposed under UV irradiation, indicating the ideal self-cleaning property of the multifunctional membrane. This novel strategy for constructing a multifunctional surface deposition layer is expected to provide broader prospects for the application of superhydrophilic membranes in oil-water separation and wastewater purification.
研究领域:
材料科学
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环境工程
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2016
·
IF 0
Zhu Xiong,Fu Liu,Haibo Lin,Jinglong Li,Yi Wang
The hemocompatibility of the dialysis membrane has been considered the key factor influencing the dialysis performance for curing the kidney malfunction. In this work, the commonly used anticoagulant heparin in the clinic was directly bonded to the poly(lactic acid) (PLA) membrane to improve the hemocompatibility via the glycidyl ether reaction. A novel precopolymer P(VP-VTES-GMA) was first prepared via free radical polymerization. The surface cross-linking of the precopolymer containing GMA segments enabled the membrane to anchor heparin through the glycidyl ether reaction. The chemical structure of the precopolymer was confirmed by 1H NMR. The surface chemistry was analyzed by XPS, toluidine blue staining, and solubility tests. The crystallization behavior, heat resistance, morphology, hydrophilicity, pore size, and pure water flux were investigated. The hemocompatibility was comprehensively investigated via APTT, PRT, PT, FIB, and platelet adhesion tests. The simulated dialysis performances relating to urea, creatinine, lysozyme, and BSA clearance were measured. All results demonstrated that the covalent bonding of heparin provided the PLA membrane with excellent hemcompatibility, hydrophilicity, heat resistance, and dialysis performance as well.
研究领域:
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2017
·
IF 4.233
Ailin Gao,Fu Liu,Zhu Xiong,Qing Yang
Superhydrophobic membranes with tunable adhesion have attracted intense interests for various engineering applications. In this work, superhydrophobic sustainable poly (lactic acid) (PLA) porous membrane with tunable adhesive force from 101 μN to 29 μN was successfully fabricated via one-step phase separation method. The incorporation of Perfluoro-1-decene (PFD) into the PLLA/PDLA membrane via the in situ polymerization can facilely tune the PLLA/PDLA stereocomplex crystallization during phase inversion, which consequently caused the unique morphology blooming evolution from bud to full-blown state. The resulted membrane showed tunable pore size, porosity, surface area, surface roughness and superhydrophobicity, which enabled the membrane with controlled-release of oil soluble drugs.
研究领域:
材料科学
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药物递送
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Hui Wang,Xiao Shi,Ailin Gao,Haibo Lin,Yongliang Chen,Yumin Ye,Jidong He,Fu Liu,Gang Deng
In the present work, we report one-step immobilization of nano-heparin coating on PLA membranes via initiated chemical vapor deposition (iCVD) for enhanced hemocompatibility. The nano-coating introduced onto the membrane surface via the crosslinking of P(MAA-EGDA) was confirmed by the FTIR, SEM and weight measurement respectively. The negative carboxyl groups could form the hydration interaction with the protein and platelets and electrostatic interaction with amide groups of thrombin by the mediation of antithrombin, which is similar but different with heparin. The P(MAA-EGDA) coated membranes showed suppressed platelet adhesion and prolonged clotting time (APTTs increased to 59 s, PTs increased to 20.4 s, TTs increased to 17.5 s, and the FIBs declined by 30 mg/dL). Moreover, the complement activation tests demonstrated the formation of C3a and C5a was inhibited. All results demonstrated that the nano-coating of P(MAA-EGDA) via iCVD significantly enhanced the hemocompatibility of PLA membranes, which is also applicable for various membranes.
研究领域:
生物材料
·
医学工程
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 25
·20492-20499
·
IF 3.289
Zhu Xiong,Fu Liu,Ailin Gao,Haibo Lin,Xuemin Yu,Yunze Wang,Yi Wang
Polylactide (PLA) has attracted much attention as a sustainable and environmentally friendly material. However, the poor heat resistance restricts its potential application as a porous membrane. Herein, a PLA membrane with excellent heat resistance, hydrophilicity and hemocompatibility was developed via a surface crosslinking induced crystallization strategy, which involved two key reactions, namely, copolymerization of N-vinyl-2-pyrrolidone (NVP) and triethoxyvinylsilane (VTES) and the subsequent hydrolysis condensation on the surface of the PLA membrane. Attenuated total reflectance Fourier transform infrared spectra (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) were applied to analyse the surface chemistry and crystallization evolution, which confirmed that the P(VP-VTES) copolymer was hydrothermally crosslinked and induced the crystallization of the PLA membrane. The surface crystallization significantly improved the heat resistance and preserved membrane morphology, which was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and the dimension shrinkage. The modified PLA membrane with χc ∼ 37% remained almost unchanged dimensions and morphology even after annealing at 100 °C for 5 min. The improved hemocompatibility was verified by the prolonged clotting time and recalcification time, which was consistent with the enhanced hydrophilicity. All results showed that surface crosslinking induced crystallization strategy simultaneously improved the heat resistance and hemocompatibility, indicating a promising method for preparing robust and compatible PLA membranes.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2016
·
IF 5.557
Jinglong Li,Fu Liu,Yan Qin,Jidong He,Zhu Xiong,Gang Deng,Qiang Li
Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4 wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin [44] . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively.
研究领域:
生物材料学
·
医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2021
·
IF 8.198
Kun Liu,Shengyue Tang,Wenyan Li,Wei Wen,Shan Ding,Mingxian Liu,Hong Li,Changren Zhou,Binghong Luo
To date, designing a perfect poly(d,l-lactide) (PDLLA) bone repair material still remains a major challenge. Inspired by the superior cell affinity, osteogenic activity, and mechanical properties of polysaccharide chitin whiskers, here, positively charged chitin whiskers (HCHW) and negatively charged maleated chitin whiskers (mCHW) are prepared for strengthening and biofunctionalizing the PDLLA membrane via a layer-by-layer (LBL) electrostatic self-assembly method. At a pH value of 4.3, the process of LBL electrostatic self-assembly between the HCHW and mCHW on the membranes is successfully performed, which can be directly proved by the cross-sectional morphology, light transmittance, surface composition, and topography of the membranes. Especially, polydopamine, as an intermediate layer, can greatly enhance the stability and adhesion of the first chitin whisker layer on the PDLLA substrate. Compared with the PDLLA membrane, the mechanical properties of self-assembly membranes are highly enhanced and controllable by adjusting the number of self-assembly whisker layers. Furthermore, in vitro biological evaluations indicate that the HCHW-assembled surface has a higher cell affinity than that of the mCHW-assembled one, which may be attributed to different protein adsorption. Meanwhile, interesting directional cell migration behavior is observed on the HCHW-assembled surface, which can be explained by the guiding effect of surface ordered topology and collective cell migration. These findings provide a new strategy for designing bioactive PDLLA composites with superior and tunable mechanical properties for bone regeneration materials.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2014
·
IF 4.908
Li-Jing Zhu,Fu Liu,Xue-Min Yu,Ai-Lin Gao,Li-Xin Xue
Poly(lactic acid) (PLA) hemodiafiltration membranes were grafted by zwitterionic polymer to improve the fouling resistance and hemocompatibility. The surface zwitterionization was accomplished by dopamine inspired bromoalkyl initiator anchoring and subsequent atom transfer radical polymerization (ATRP) of zwitterionic poly(sulfobetaine methacrylate) (PSBMA). Attenuated total reflectance Fourier transform infrared spectra (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) were applied to analyze the surface chemistry and morphology evolution of the fabricated membranes, which confirmed that zwitterionic PSBMA was successfully grafted on PLA membrane. The results of water contact angle and zeta potentials demonstrated that the hydrophilicity and negativity of the modified PLA membranes were highly improved, which effectively alleviated the surface adsorption of bovine serum albumin (BSA) and palates. Moreover, the modified PLA membrane exhibited good dialysis performances. When the polymerization time of PSBMA was 1 h, the water flux was 184 L m −2 h −1 , while BSA retention reached up to 90%, and the clearance of urea and creatinine was maintained at 66% and 60%, respectively. The present work provides a convenient strategy to improve the hydrophilicity, anti-fouling and hemocompatibility of PLA membranes for their biomedical and blood-contacting applications such as hemodialysis.
研究领域:
生物医学工程
·
材料科学
·
血液净化技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2013
·
IF 4.093
Ailin Gao,Fu Liu,Lixin Xue
Bio-based poly (lactic acid) membrane with asymmetric porous structure was developed for hemodialysis via phase inversion for the first time. Heparin was immobilized to PLA membrane surface through the strong adhesion ability of dopamine, as confirmed by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) respectively. The morphologies variations of PLA membranes induced by dopamine coating and heparin immobilization were analyzed by scanning electron microscope (SEM) and atomic force microscopy (AFM). Hydrophilicity and permeability of PLA membranes before and after modification were characterized. Particularly, platelet adsorption, plasma recalcification time and hemolysis ratio were executed to evaluate the blood compatibility of PLA membranes decorated by heparin. The in vitro results demonstrated that surface heparinization improved the hemocompatibility of PLA membrane, suppressed the adhesion of platelet, extended plasma recalcilication time, and also decreased hemolysis ratio. The dialysis simulation experiments including urea and lysozyme clearance as well as bovine serum albumin (BSA) rejection were implemented to determine the dialysis performances. The results showed that 79% of urea and 18% of lysozyme were cleaned and over 90% of BSA was retained. This study disclosed a window of opportunity to produce novel hemodialysis membranes using bio-based materials.
研究领域:
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）