Polymers
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2023
·Vol. 15
·期 7
·1755
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IF 5
Junyuan Zhang,Zilong Peng,Mengjie Wang,Yinan Li,Jinyin Wu,Yifan Jiang,Chaolong Liu,Guqiang Li,Lin Xu,Hongbo Lan
The fabrication of various 3D tissue engineering tubular scaffolds with fibrous structures, to assist the human body in rapidly repairing a variety of ailments, is receiving more and more attention. Due to the inefficiency of the majority of fibrous preparation techniques, the question of how to rapidly produce the requisite three-dimensional tubular microfiber scaffold structures has become an urgent problem. In this study, an efficient polymer fiber preparation method was developed, using a high-speed airflow drive. Melt blending of polycaprolactone (PCL), polylactic acid (PLA), and tributyl citrate (TBC), was used for the printing material, to achieve the efficient preparation of tubular microfiber scaffolds with different structures. The scaffold diameter was as small as 2 mm, the wall thickness was up to 100 μm, and the fiber injection efficiency reached 15.48 g/h. By utilizing simulations to optimize the printing parameters and by adjusting the printing settings, it was possible to achieve a controlled fiber diameter in the range of 3 μm to 15 μm. In addition, plasma treatment was applied to the microfibers’ surface, to increase their wettability, and the efficiency of the hydrophilic modification was demonstrated. Furthermore, the mechanical property test demonstrated that the fibers have a tensile strength of 1.36 ± 0.16 MPa and a tensile strain of 30.8 ± 3.5%. The radial compressive strain of the tubular scaffold could reach 60% of the original scaffold’s diameter. Finally, the in vitro degradation of the fibers at various pH values was tested. The results showed that, under alkaline conditions, the surface of the fibers would be severely crushed and the rate of deterioration would increase.Keywords:airflow-field-driven;3D printing;microfiber scaffold;polycaprolactone (PCL);polylactic acid (PLA)
研究领域:
组织工程
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材料科学
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生物医学工程
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2017
·
IF 3.887
Delin Cheng,Qingfei Liang,Yonggang Li,Jiahui Fan,Guocheng Wang,Haobo Pan,Changshun Ruan
Although heterogeneous bone scaffolds have shown potential in bone defect repair, their capability of aiding bone regeneration need to be further enhanced. Strontium, one important trace element in bone, has a well-known favorable effect on bone repair. Here a strontium containing scaffold (CPB/PCL/Sr) based on superficially porous calcined porcine bone (CPB) was obtained straightforwardly by sequential coating of SrCl 2 and polycaprolactone (PCL). The basic characterization revealed that PCL coating could simultaneously improve the mechanical properties and, more importantly, restrain strontium release. Moreover, in vitro behaviors of human MSCs on CPB, CPB/PCL, and CPB/PCL/Sr were studied in detail. The comprehensive results of proliferation, osteogenic gene expression, ALP staining, and ALP activity demonstrated that PCL coating slightly impaired the bone repair potential of CPB. In contrast, CPB/PCL/Sr better supported the osteogenic differentiation of MSCs than CPB，highlighting the role of strontium. The in vivo test confirmed a better new bone formation of CPB/PCL/Sr than CPB and CPB/PCL. These results verified the superiority of incorporating strontium to improve the bone-forming ability of CPB, offering a promising alternative for bone defect repair.
研究领域:
生物医学工程
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组织工程
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再生医学
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Decong Kong,Meiqi Song,Yonghong Pan,Xierzhati Aimaier,Ye Tian,Rui Yang,Jing Huang,Yi Liu,Xiaomei Liu,Nikolai Boshkov,Botao Zhang,Hua Li
In this study, the interface-modified ternary blends based on polylactic acid (PLLA)-polycaprolactone (PCL)-polycarbonate diols (PCDL) were prepared via suspension flame spray. Morphological analysis showed that the interface modification of PCDL significantly improves the compatibility of PLLA and PCL. Mechanical analysis revealed that the addition of PCDL resulted in great enhancement of the toughness of the PLLA/PCL composite coatings with a dramatic 326 % increase of the fracture strain for 10PLLA/90PCL/10PCDL compared with 10PLLA/90PCL. Further physicochemical and antibacterial test revealed no significant difference among PLLA/PCL and PLLA/PCL/CAM composite coatings with and without PCDL. Time-course release analysis showed that the release mechanism of the composite coating generally remains the same when the PCDL added is lower than the main components in PLLA/PCL, and even changed from the original abnormal diffusion to Fick diffusion. Overall, the ternary blending approach of PLLA-PCL-PCDL using suspension flame spray is a promising technique with great potential drug-delivery applications with different mechanical requirements.
研究领域:
材料科学
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生物医学工程
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制药工程
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DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2019
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IF 3.973
Chenglong Yu,Meiyi Xing,Shibo Sun,Guoping Guan,Lu Wang
Vascular basement membrane (VBM) is a thin layer of fibrous extracellular matrix linking endothelium, and collagen type IV (COL IV) is its main composition. VBM plays a crucial role in anchoring down the endothelium to its loose connective tissue underneath. For vascular grafts, constructing biomimetic VBMs on the luminal surface is thus an effective approach to improve endothelialization in situ . In the present work, three types of polycaprolactone (PCL) membranes were produced and characterized through cell counting kit-8 (CCK-8) assay, adhesion force and elastic modulus test to examine the influence of fiber diameter and membrane composition on vascular endothelial cell (EC) behaviors. The PCL membranes with finer fibers of 54.77 nm (PCL-54) could biomimic the nanotopography of VBMs more efficiently than 544.64 nm (PCL-544), and they were more suitable for Pig iliac endothelium cells (PIECs) adhesion and proliferation, meanwhile, inducing higher elastic modulus and adhesion force of PIECs. On this foundation, we further immobilized COL IV onto PCL-54 (PCL-COL IV) to biomimic VBMs compositionally. Results showed that PIECs on PCL-COL IV exhibited the highest viability and proliferation. Besides, quantitative data indicated that the elastic modulus of the PIECs on PCL-COL IV (4441.00 Pa) was as two times higher than that on PCL-54 (2312.26 Pa), and the adhesion force grew to 1120.99 pN from 673.58 pN of PIECs on PCL-54. In summary, the PCL-COL IV membranes show high similarity with the native VBMs in terms of structure and composition, suggesting a promising potential for surface modification to vascular grafts for improved endothelialization.
研究领域:
组织工程
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生物材料
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细胞生物学
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DOI
PubMed
高影响因子
Journal of Materials Research and Technology-JMR&T
·
2022
·
IF 6.267
Wentao Shi,Zhijia Shen,Lu Bian,Yan Gao,Yiqing Wu,Feifei Zhao,Hong Tang,Xiaojie Lu
3,4-Dihydroxyphenylalanine (DOPA) is believed to play an essential role in the solidification and adhesion of liquid mussel adhesives; this thin polymer layer very tightly adheres to the surface of scaffolds, making it stable enough to prevent it from being peeled off. Fascinatingly, the deposition of DOPA on various types of surfaces via the self-polymerization of DOPA under alkaline conditions is a simple approach for creating biomimetic coatings. In this study, DOPA derivatives were used as monomers to form primary coatings on polycaprolactone (PCL) surfaces. After that, a second chemical crosslinking method was utilized to graft alendronate (AL) on the DOPA coatings via genipin (GP) crosslinking ( [email protected] ). X-ray photoelectron spectroscopy, Fourier transform infrared reflection and scanning electron microscopy analyses indicated that AL was distributed homogeneously on the surface of PCL. This new organic–inorganic hybridized coating could support EMSC growth and osteogenic differentiation. Additionally, animal studies were also performed to verify the function of [email protected] in promoting osteogenesis. The method is simple, cheap and highly efficient, which makes it very convenient to apply on a large scale.
研究领域:
材料科学
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生物材料
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组织工程
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生物医学工程
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产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2020
·Vol. 10
·期 3
·2000604
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IF 7.367
Jin Zhang,Xi Zhang,Chenyu Wang,Feihan Li,Ziwen Qiao,Liangdan Zeng,Zhonghan Wang,He Liu,Jianxun Ding,Huanghao Yang
Conductivity and alignment of scaffolds are two primary factors influencing the efficacy of nerve repair. Herein, conductive composite fibers composed of poly(ɛ-caprolactone) (PCL) and carbon nanotubes (CNTs) with different orientation degrees are prepared by electrospinning at various rotational speeds (0, 500, 1000, and 2000 rpm), and meanwhile the synergistic promotion mechanism of aligned topography and electrical stimulation on neural regeneration is fully demonstrated. Under an optimized rotational speed of 1000 rpm, the electrospun PCL fiber exhibits orientated structure at macroscopic (mean deviation angle = 2.78°) or microscopic crystal scale (orientation degree = 0.73), decreased contact angle of 99.2° ± 4.9°, and sufficient tensile strength in both perpendicular and parallel directions to fiber axis (1.13 ± 0.15 and 5.06 ± 0.98 MPa). CNTs are introduced into the aligned fiber for further improving conductivity (15.69–178.63 S m −1 ), which is beneficial to the oriented growth of neural cells in vitro as well as the regeneration of injured sciatic nerves in vivo. On the basis of robust cell induction behavior, optimum sciatic nerve function index, and enhanced remyelination/axonal regeneration, such conductive PCL/CNTs composite fiber with optimized fiber alignment may serve as instructive candidates for promoting the scaffold- and cell-based strategies for neural repair.
研究领域:
材料科学
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神经科学
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组织工程
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Min He,Qian Wang,Li Xie,Hao Wu,Weifeng Zhao,Weidong Tian
Functional graded nanofibrous membrane (FGM) are gaining great attention in biomedical fields for the reason that it allows tunning the composition, structure, and bioactivity of each layer. Herein, we reported a novel FGM prepared by sequential electrospinning technique to guide bone tissue regeneration. The FGM consisted of two aligned gelatin nanofiber surface layers to improve the biocompatibility and enhance bone regeneration, and a random PCL nanofiber core layer to render the membrane isotropic mechanical strength. To eliminate bacterial infection risk, the pro-metronidazole (Pro-MNA) with infection-responsive release property was synthesized and loaded into the outer layer. The FGM displayed comparable mechanical strength with PCL, and similar biocompatibility to gelatin. The surface aligned nanofiber structure could regulate epithelial cell spread along the aligned direction, instead of infiltration growth, and were more beneficial for the migration and proliferation of osteoblast. In addition, the loaded Pro-MNA displayed infection-responsive release behavior, and robust self-defensive antibacterial function was achieved. These results suggested that the FGM simultaneously fulfil several criteria of biocompatibility, space maintaining capability, antibacterial property, and osteoconductivity. Consequently, the FGM displayed better bone regeneration performance than the commercial Bio-Gide membrane, evaluated by the skull defect model of rabbit. We concluded that the FGM had potential to be used as an ideal GTRM due to its improved overall performance.
研究领域:
生物医学工程
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组织工程
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材料科学
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再生医学
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产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2015
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IF 4.383
Xiang Gao,Xiaohong Zhang,Jinlin Song,Xiao Xu,Anxiu Xu,Mengke Wang,Bingwu Xie,Enyi Huang,Feng Deng,Shicheng Wei
The construction of functional biomimetic scaffolds that recapitulate the topographical and biochemical features of bone tissue extracellular matrix is now of topical interest in bone tissue engineering. In this study, a novel surface-functionalized electrospun polycaprolactone (PCL) nanofiber scaffold with highly ordered structure was developed to simulate the critical features of native bone tissue via a single step of catechol chemistry. Specially, under slightly alkaline aqueous solution, polydopamine (pDA) was coated on the surface of aligned PCL nanofibers after electrospinning, followed by covalent immobilization of bone morphogenetic protein-7-derived peptides onto the pDA-coated nanofiber surface. Contact angle measurement, Raman spectroscopy, and X-ray photoelectron spectroscopy confirmed the presence of pDA and peptides on PCL nanofiber surface. Our results demonstrated that surface modification with osteoinductive peptides could improve cytocompatibility of nanofibers in terms of cell adhesion, spreading, and proliferation. Most importantly, Alizarin Red S staining, quantitative real-time polymerase chain reaction, immunostaining, and Western blot revealed that human mesenchymal stem cells cultured on aligned nanofibers with osteoinductive peptides exhibited enhanced osteogenic differentiation potential than cells on randomly oriented nanofibers. Furthermore, the aligned nanofibers with osteoinductive peptides could direct osteogenic differentiation of human mesenchymal stem cells even in the absence of osteoinducting factors, suggesting superior osteogenic efficacy of biomimetic design that combines the advantages of osteoinductive peptide signal and highly ordered nanofibers on cell fate decision. The presented peptide-decorated bone-mimic nanofiber scaffolds hold a promising potential in the context of bone tissue engineering.
研究领域:
生物材料
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组织工程
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生物医学工程
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干细胞生物学
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DOI
PubMed
高影响因子
Biomaterials Advances
·
2025
·
IF 6
Ying Sun,Yining Cai,Shanshan Xu,Ningning Zhai,Zirui Wang,Zongpu Qiu,Shuying Han,Yuanqing Wei,Rui Liu,Hao Wu
While the regulatory roles of biomaterial topology in bone regeneration are recognized, the specific osteogenic mechanisms driven by distinct morphological features, particularly without biochemical cues, remain poorly understood. This study aimed to independently elucidate the osteogenic potential and underlying mechanisms solely attributable to the distinctive “S”-grooved topology derived from cuttlebone. Inspired by cuttlebone, we successfully fabricated a biomimetic polycaprolactone (PCL) membranes sheet with “S”-grooved topology using liquid silicone mold replication combined with PCL recasting techniques. Comprehensive characterization (SEM, CLSM) confirmed precise replication of the native “S”-grooved morphology (width: 100 ± 30 μm, depth: 20 ± 6 μm). In vitro studies revealed that these grooves promoted directional adhesion and proliferation of rat bone marrow mesenchymal stem cells (rBMSCs) via contact guidance, as evidenced by cytoskeleton alignment. Furthermore, the “S”-grooved topology significantly enhanced osteogenic differentiation of mouse pre-osteoblasts (MC3T3-E1), demonstrated by increased alkaline phosphatase (ALP) activity and mineralized nodule formation. Mechanistic investigations using RT-qPCR, Western blot, and dimethyl labeling-based quantitative proteomics on rBMSCs identified key involvement of the Rap1-ERK signaling pathway. Specifically, the “S”-grooved topology upregulated Rap1 expression and enhanced ERK phosphorylation, leading to increased expression of osteogenic markers (e.g., Runx2, OPN). These findings demonstrate that the biomimetic “S”-grooved topology alone, through contact guidance and activation of the Rap1-ERK mechanotransduction pathway, significantly enhances osteogenic differentiation, providing a foundation for designing topologically optimized biomaterials for bone regeneration biomimetic PCL membranes sheet with the “S”-grooved topology.
研究领域:
生物材料
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组织工程
·
细胞生物学
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生物医学工程
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材料科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2024
·
IF 5.5
Changgi Hong,Haeun Chung,Gyubok Lee,Dongwoo Kim,Zhuomin Jiang,Sang-Heon Kim,Kangwon Lee
Wound dressings made from natural-derived polymers are highly valued for their biocompatibility, biodegradability, and biofunctionality. However, natural polymer-based hydrogels can come with their own set of limitations, such as low mechanical strength, limited cell affinity, and the potential cytotoxicity of cross-linkers, which delineate the boundaries of their usage and hamper their practical application. To overcome the limitation of natural-derived polymers, this study utilized a mixture of oxidized alginate and gelatin with 5 mg/mL polycaprolactone (PCL):gelatin nanofiber fragments at a ratio of 7:3 (OGN-7) to develop a hydrogel composite wound dressing that can be injected and has the ability to be remended. The in situ formation of the remendable hydrogel is facilitated by dual cross-linking of oxidized alginate chains with gelatin and PCL/gelatin nanofibers through Schiff-base mechanisms, supported by the physical integration of nanofibers, thereby obviating the need for additional cross-linking agents. Furthermore, OGN-7 exhibits increased stiffness (γ = 79.4–316.3%), reduced gelation time (543 ± 5 to 475 ± 5 s), improved remendability of the hydrogel, and excellent biocompatibility. Notably, OGN-7 achieves full fusion within 1 h of incubation and maintains structural integrity under external stress, effectively overcoming the inherent mechanical weaknesses of natural polymer-based dressings and enhancing biofunctionality. The therapeutic efficacy of OGN-7 was validated through a full-thickness in vivo wound healing analysis, which demonstrated that OGN-7 significantly accelerates wound closure compared to alginate-based dressings and control groups. Histological analysis further revealed that re-epithelialization and collagen deposition were markedly enhanced in the regenerating skin of the OGN-7 group, confirming the superior therapeutic performance of OGN-7. In summary, OGN-7 optimized the synergistic effects of natural polymers, which enhances their collective functionality as a wound dressing and expands their utility across diverse biomedical applications.
研究领域:
生物医学工程
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材料科学
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组织工程
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伤口愈合
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF NEUROSCIENCE
·
2017
·Vol. 45
·期 10
·1258-1267
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IF 2.941
Dan Lv,Lijuan Zhou,Xianyu Zheng,Yongcheng Hu
Although the peripheral nervous system has an intrinsic ability for repair and regeneration after injury, bridging long peripheral nerve defects remains a challenge. Functional nerve regeneration depends on interactions among axons, Schwann cells, fibroblasts and immune cells. Macrophages, as immune cells recruited early in this process, show polarization toward phenotypes that are detrimental or beneficial to tissue remodeling depending on the microenvironment of the scaffolds. In this study, we investigated the effects of macrophage phenotypes modulated by collagen VI on axonal regeneration and functional recovery by bridging a 15-mm-long sciatic nerve defect in rats. Our results showed that local delivery of collagen VI within a polycaprolactone (PCL) electrospun conduit increased the recruitment of macrophages and their polarization toward the pro-healing (M2) phenotype. In addition, the axonal regeneration and neurologic functional recovery in the PCL/collagen VI conduit group are equivalent to that of an autograft. In conclusion, the present study confirmed that PCL/collagen VI conduits with sustained release of collagen VI in the local microenvironment may, through triggering macrophage M2 polarization to enhance the nerve regeneration, suggest that our combined biomaterial-immunomodulatory system may be an attractive candidate for peripheral nerve regeneration.
研究领域:
神经科学
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组织工程
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免疫学
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生物材料
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产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2018
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IF 5.08
Yiqun Wang,Yi Lu,Jinkang Gong,Yuan Yao
Intermediate filaments, together with actin microfilaments and microtubules constituent the cytoskeleton of mammalian cells, involving in various cellular activities. The roles of intermediate filaments in cell skeleton reorganization when responding with extracellular matrix (ECM) nanostructure are poorly understood yet. To unveil the effects of fibrous composition and orientation on cells, we developed electrospun nanofibers of varying topology and components, and the effects on assembly of intermediate filaments as keratin and vimentin were investigated in detail. We found that aligned nanofibers enhanced expression of E-cadherin and promoted assembly of keratin intermediate filaments. Meanwhile, the compositional variation show different preference on up-regulation of the two intermediate filaments. Compared to keratin, the assembly of vimentin intermediate filaments were promoted by incorporating bovine serum albumin (BSA) functionalized graphene oxide (BSA-GO) into polycaprolactone (PCL) nanofibers. Thus, our findings elucidate how the different physical factors of fibrous extracellular matrix affect the reorganization of cytoskeleton by assembly of keratin and vimentin intermediate filaments.
研究领域:
材料科学
·
细胞生物学
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癌症研究
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）