CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Feng Ling,Xiayu Hu,Yujie Shen,Xi Chen,Zonglai Qian,Hao Liu,Yong Xu,Fan He
Nucleotomy, a surgical procedure employed to address nucleus pulposus (NP) herniation, frequently results in defects within the annulus fibrosus (AF). Owing to the inherently limited self-repair ability of AF tissue, untreated lesions may precipitate reherniation of the NP, thereby accelerating the degeneration of the intervertebral disc. In this study, we introduce a novel composite scaffold comprising hydrogel-reinforced electrospun nanofibers, engineered to mimic the heterogeneous microarchitecture of the native AF matrix while offering improved mechanical support. A mesh-like polycaprolactone (PCL)/gelatin methacryloyl (GelMA) hybrid electrospun nanofibrous membrane was designed to replicate the AF outer region, while a hydrogel mixture of fucoidan methacryloyl (FuMA) and GelMA was sprayed to the membrane surface to emulate the inner region. The integrated FuMA/GelMA hydrogel and PCL/GelMA nanofiber layer enhanced the proliferation of AF cells and markedly improved the synthesis of AF matrix components. Importantly, even under oxidative stress induced by hydrogen peroxide, this hydrogel-reinforced nanofibrous composite effectively maintained the mitochondrial functions of AF cells, thereby supporting their energy metabolism and matrix anabolism. Further molecular experiments unraveled that this composite protected AF cells from oxidative damage through activation of the NRF2-mediated antioxidant enzymes, such as heme oxygenase 1. In a rat caudal disc box defect model, the implantation of a hydrogel-reinforced nanofibrous composite promoted the regeneration of AF tissue, preserved the hydration of NP tissue, and inhibited intervertebral disc degeneration. This approach represents a promising strategy for the repair of nucleotomy-induced AF damage, thereby offering potential therapeutic benefits for patients experiencing intervertebral disc degeneration.
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高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Shue Jin,Jing Wen,Yao Zhang,Ping Mou,Zeyu Luo,Yongrui Cai,Anjin Chen,Xiaoxue Fu,Weikun Meng,Zongke Zhou,Jidong Li,Weinan Zeng
Designing scaffolds that can regulate the innate immune response and promote vascularized bone regeneration holds promise for bone tissue engineering. Herein, electrospun scaffolds that combined physical and biological cues were fabricated by anchoring reparative M2 macrophage–derived exosomes onto topological pore structured nanofibrous scaffolds. The topological pore structure of the fiber and the immobilization of exosomes increased the nanoscale roughness and hydrophilicity of the fibrous scaffold. In vitro cell experiments showed that exosomes could be internalized by target cells to promote cell migration, tube formation, osteogenic differentiation, and anti-inflammatory macrophage polarization. The activation of fibrosis, angiogenesis, and macrophage was elucidated during the exosome-functionalized fibrous scaffold–mediated foreign body response (FBR) in subcutaneous implantation in mice. The exosome-functionalized nanofibrous scaffolds also enhanced vascularized bone formation in a critical-sized rat cranial bone defect model. Importantly, histological analysis revealed that the biofunctional scaffolds regulated the coupling effect of angiogenesis, osteoclastogenesis, and osteogenesis by stimulating type H vessel formation. This study elaborated on the complex processes within the cell microenvironment niche during fibrous scaffold–mediated FBR and vascularized bone regeneration to guide the design of implants or devices used in orthopedics and maxillofacial surgery. Statement of significance How to design scaffold materials that can regulate the local immune niche and truly achieve functional vascularized bone regeneration still remain an open question. Here, combining physical and biological cues, we proposed new insight to cell-free and growth factor-free therapy, anchoring reparative M2 macrophage–derived exosomes onto topological pore structured nanofibrous scaffolds. The exosomes functionalized-scaffold system mitigated foreign body response, including excessive fibrosis, tumor-like vascularization, and macrophage activation. Importantly, the biofunctional scaffolds regulated the coupling effect of angiogenesis, osteoclastogenesis, and osteogenesis by stimulating type H vessel formation.
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高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Ruinan Hao,Xilin Ye,Xiaofeng Chen,Jinzhi Du,Feng Tian,Liqun Zhang,Guolin Ma,Feng Rao,Jiajia Xue
Skin wound healing is a complex process that requires appropriate treatment and management. Using a single scaffold to dynamically manipulate angiogenesis, cell migration and proliferation, and tissue reconstruction during skin wound healing is a great challenge. We developed a hybrid scaffold platform that integrates the spatiotemporal delivery of bioactive cues with topographical cues to dynamically manipulate the wound-healing process. The scaffold comprised gelatin methacryloyl hydrogels and electrospun poly(ε-caprolactone)/gelatin nanofibers. The hydrogels had graded cross-linking densities and were loaded with two different functional bioactive peptides. The nanofibers comprised a radially aligned nanofiber array layer and a layer of random fibers. During the early stages of wound healing, the KLTWQELYQLKYKGI peptide, which mimics vascular endothelial growth factor, was released from the inner layer of the hydrogel to accelerate angiogenesis. During the later stages of wound healing, the IKVAVS peptide, which promotes cell migration, synergized with the radially aligned nanofiber membrane to promote cell migration, while the nanofiber membrane also supported further cell proliferation. In an in vivo rat skin wound-healing model, the hybrid scaffold significantly accelerated wound healing and collagen deposition, and the ratio of type I to type III collagen at the wound site resembled that of normal skin. The prepared scaffold dynamically regulated the skin tissue regeneration process in stages to achieve rapid wound repair with clinical application potential, providing a strategy for skin wound repair.
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高影响因子
Materials Today Bio
·
2026
·
IF 11
Xinyue Guan,Siyu Xu,Wenxin Meng,Quanli Li,Yuhui Liu,Chuan Wu,Zhongrong Chen,Guomin Wu
The repair of large-scale bone defects remains a significant challenge in clinical orthopedics, often complicated by bacterial infection and limited self-healing capacity. Therefore, developing artificial scaffolds that can simultaneously prevent infection and orchestrate a regenerative microenvironment is critical for successful therapy. Herein, a hierarchical 3D scaffold (CaSiO 3 /PCL NFs + BP@PCL MSs) integrating calcium silicate (CaSiO 3 )-doped polycaprolactone (PCL) nanofibers and black phosphorus (BP)-encapsulated microspheres is engineered via combined electrospinning and electrospraying techniques. This design constructs a biomimetic architecture with dual photothermal-ionic regulation capabilities. Under near-infrared (NIR) irradiation, the scaffold exhibits superior photothermal conversion, which rapidly eradicates bacteria while accelerating the release of therapeutic ions to establish a pro-mineralization niche. Further in vitro and in vivo evaluations demonstrate that the scaffold exhibits excellent in situ mineralization capability while simultaneously promoting cell proliferation, migration, and osteogenic differentiation. Furthermore, the implanted scaffold effectively promotes vascularized bone regeneration at defect sites. This strategy, synergizing photothermal therapy with an optimized ionic microenvironment, provides a promising approach for bone tissue engineering applications.
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高影响因子
Biomimetics
·
2026
·Vol. 11
·期 8
·599
·
IF 4.2
Haiguang Zhang,Feng Jiang,Qianmin Gao,Qingxi Hu,Jiaxuan Feng
Vascular stent implantation is a major treatment for vascular diseases, yet postoperative infection and persistent inflammation increase the risk of in-stent restenosis. Herein, core–shell structured PCL/PEO-PVP fiber membranes co-loaded with ciprofloxacin hydrochloride (CIP) and curcumin (CUR) were fabricated via coaxial electrospinning. Orthogonal experiments were conducted to optimize critical spinning parameters through multi-index comprehensive evaluation. Characterizations confirm intact core–shell architecture and stable polymeric backbone structure. In vitro release tests reveal sequential drug-delivery behavior: a rapid initial release of hydrophilic CIP and a delayed sustained release of hydrophobic CUR were observed, contributing to early-stage antibacterial and long-term anti-inflammatory effects, respectively. The “antibacterial zone” method verifies favorable antibacterial activity againstStaphylococcus aureus(S. aureus) andEscherichia coli(E. coli). ELISA results demonstrate the enhanced anti-inflammatory capacity of the dual-drug-loaded coaxial fiber membrane. Cellular assays confirm satisfactory cytocompatibility, and endothelial cells achieve normal proliferation and exhibit typical polygonal morphology on the membrane surface. This dual-drug-loaded coaxial-fiber membrane realizes coordinated sequential antibacterial and anti-inflammatory properties, which provides a feasible strategy for developing functional coatings toward vascular stents.
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ACS Applied Materials & Interfaces
·
2026
·Vol. 18
·期 33
·44984-45002
·
IF 7.8
Wang Shuaihang,Ji Zhonghao,Shen Ding,Liu Siyu,Hao Jingbin,Cheng Qi,Jia Bao,Guo Liang,Shang Jie,Chen Kai,Han Meng
Currently, the repair of bone defects faces multiple challenges, including insufficient angiogenesis and limited osteogenic differentiation capacity, which pose significant obstacles to treatment. In this study, a ternary composite scaffold composed of PCL-nHA-nMgO (PHM) was fabricated using 3D printing technology. After surface modification with a photothermal polydopamine (PDA) coating, a PHM@PDA composite scaffold was obtained. Metformin (Met) and PDA were loaded into GelMA hydrogels to produce GPM hydrogels. The GPM hydrogels were then packed into the PHM@PDA composite scaffolds, resulting in near-infrared region-I (NIR-I)-responsive PHM@PDA/GPM composite scaffolds. The outer GPM layer serves as a three-dimensional in vivo-like microenvironment, providing physical transport channels for cellular nutrient exchange. Upon stimulation by NIR-I, the scaffold enables the dynamic release of Mg 2+ and Met, upregulates the expression of the VEGF and KDR, accelerates vascular network reconstruction, and creates a mild microenvironment conducive to cell proliferation. Furthermore, by activating the PI3K/AKT signaling pathway, it promotes the expression of osteogenesis-related factors (such as transcription factor Sp7, alkaline phosphatase, and osteocalcin), thereby accelerating the osteogenic differentiation process. In vivo animal experiments, micro-CT and tissue staining confirmed that, under near-infrared light (NIR-I) stimulation, this scaffold significantly increased bone mineral density, trabecular bone thickness, and trabecular bone count and demonstrated excellent angiogenic effects. In summary, this NIR-I-responsive composite scaffold can meet the dual requirements of reconstructing the vascular network and promoting osteogenic differentiation, enabling personalized precision therapy and offering a promising new treatment strategy for complex bone defects where vascular regeneration and osteogenic differentiation are challenging.
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ACS Applied Bio Materials
·
2026
·Vol. 9
·期 15
·7069-7080
·
IF 5.6
Chi Chunyu,Gao Yuantao,Chen Jiazhu,Ge Xuejun,Gao Zexing,Li Yinggang,Yan Shuang
The delayed healing of skin wounds poses significant health risks and increases medical burdens, necessitating the development of multifunctional dressings that can actively monitor and promote the healing process. In this study, a multifunctional CeO 2 /PCL nanofibrous scaffold integrating real-time pH sensing, enhanced stem cell adhesion, and antioxidant functions was developed. A series of composite scaffolds with tunable CeO 2 nanoparticle loadings (7.1–31.6 wt %) were fabricated via a facile one-step electrospinning technique. Notably, the CeO 2 /PCL-13.3 wt % scaffold achieved an optimal balance of tensile strength (1.927 MPa) and elongation at break (70%), fully satisfying clinical wound dressing requirements. Phenol red loading enabled naked-eye pH monitoring (4.0–10.0) via metal−phenolic coordination. Comprehensive evaluations demonstrated that the composite scaffolds exhibited significant antibacterial activity, potent reactive oxygen species scavenging capability, and excellent biocompatibility. The scaffolds maintained over 80% human umbilical cord mesenchymal stem cells viability within 72 h and significantly promoted stem cell migration in wound healing assays. This integrated platform unifies visual diagnostics and therapeutic functions for advanced wound management.
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International Journal of Nanomedicine
·
2026
·
IF 8.7
Congcong Yang,Jianyou Xia,Lunjie Zhao,Jianping Tao,Dan Li,Renxi Zhu,Qiang Wang,Haichen Shen,Baochao Zhang,Yujie Xu
Background In recent years, bladder defect repair has emerged as a critical issue in urological tissue engineering. Traditional treatment methods, such as autologous tissue transplantation and synthetic material repair, are limited by factors such as scarce donor sources, immune rejection, and postoperative fibrosis. Consequently, the development of nanofiber materials with bionic structures, biocompatibility, and anti-fibrotic capabilities has become a research hotspot. This research addressed the clinical needs associated with tuberculous bladder contracture, chronic cystitis, traumatic bladder rupture, and malignant tumors requiring partial cystectomy (such as localized non-muscle-invasive bladder cancer and urachal cancer), among other conditions. Excessive fibrotic scar formation following bladder surgery or injury is a primary contributor to reduced bladder compliance, diminished capacity, and impaired contractile function.Methods Using electrospinning technology, we designed and prepared composite nanofibers with varying proportions (9:1, 7:3, 5:5) of polycaprolactone (PCL) and gelatin (GEL). By conducting various experiments such as scanning electron microscopy (SEM), water contact angle (WCA) analysis, mechanical performance evaluation, and Fourier transform infrared spectroscopy (FTIR), the PCL/GEL (7:3) composite material was ultimately selected as the one with the best overall performance.Results Its fiber diameter was 612.14 ± 105.46 nm, water contact angle was 107.23°, and mechanical properties (tensile strength: 3.84 ± 0.5 MPa, elongation at break: 118.42 ± 4%, Young’s modulus: 19.50 ± 4.6 MPa). To enhance its anti-fibrotic properties, we incorporated mitomycin C (MMC) into the nanofiber matrix and prepared PCL/GEL/MMC nanofiber materials through blending and spinning. We then established a partial cystectomy model in rats, implanted the PCL/GEL/MMC nanofiber materials, and performed bladder imaging four weeks post-surgery to assess bladder capacity and morphological recovery. The CCK-8 assay was performed on days 1, 3, and 7, demonstrating that smooth muscle cells (SMCs) and endothelial cells (ECs) can effectively adhere, survive, and proliferate on these fibrous membranes, thereby confirming their biocompatibility. The anti-fibrotic properties of the materials were evaluated using immunofluorescence staining (IF)and immunohistochemical analysis (IHC).Conclusion The experimental results demonstrated that PCL/GEL nanofiber materials loaded with 0.02% MMC exhibited excellent biocompatibility and anti-fibrotic effects in bladder defect repair, providing a theoretical basis for their potential clinical application.
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ACS Applied Engineering Materials
·
2026
·
IF 3.5
Rui Fang,Weiqi Xu,Chen Yuan,Di Sun,Yanchuan Guo,Yisheng Xu
To address the challenge of antibiotic resistance in wound healing, we developed a functional electrospun fiber membrane utilizing a nonantibiotic antimicrobial strategy. Zinc phthalocyanine (ZnPc), a potent photosensitizer, was encapsulated into NPs exhibiting dual photothermal and photodynamic properties via flash nanoprecipitation (FNP). The resulting ZnPc nanoparticles (NPs) demonstrated a high photothermal conversion efficiency of 34.6% and robust photodynamic activity under 660 nm laser irradiation. These nanoparticles were incorporated into a polycaprolactone/gelatin (ZnPc-PG) nanofibrous membrane. The membrane demonstrated 99% bactericidal efficiency against Staphylococcus aureus and Escherichia coli within 5 min of laser irradiation through synergistic photothermal and photodynamic therapies. In vivo experiments confirmed that the ZnPc-PG membrane effectively accelerated wound healing and suppressed inflammatory responses in infected wounds while exhibiting excellent biocompatibility. This study provides an effective strategy for developing functional wound dressings for treating bacterial wound infections.
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Advanced Composites and Hybrid Materials
·
2026
·
IF 15.5
Bai Tian,Qin Xionghai,Wu Zhenguo,Su Junjie,Li Lin,Yao Hang,Niu Zhaoxuan,Yue Chengbin,Wang Dong,Yue Yiying,Cheng Wanli,Zeng Chengjun,Zhao Wei,Han Guangping
Commercial airway stents often slip and deform, leading to severe complications such as mucosal injury, granulation tissue formation, and stent migration, which can cause recurrent airway obstruction and necessitate repeated interventions. Here, we introduce a 3D-printed tracheal stent composed of continuous bamboo fiber reinforced shape memory polylactic acid (BF/SMP). These naturally derived fibers (tensile strength ~ 1.90 GPa, modulus ~ 91.30 GPa) exhibit excellent mechanical resilience. Following an instantaneous thermal treatment, their interfacial compatibility with SMP is notably improved. The 30% BF/SMP stent can undergo over 1,000 three-point bending cycles under a 20 N load, with a bending deflection of 30.85%. In vitro, it promotes bronchial epithelial cell proliferation. Preliminary animal studies suggest that the biomass-based stent provides airway support without causing significant inflammation or organ abnormalities. Overall, this work demonstrates a biomass-reinforced, thermally deployable airway stent concept and provides a foundation for future studies on catheter-compatible delivery and long-term in vivo performance.
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LANGMUIR
·
2026
·
IF 4.4
Penghong Pang,Xiuhua Sun,Changlu Gao
In this study, amphiphilic copolymers with biocompatible and electrically neutral poly(2-hydroxyethyl methacrylate) (PHEMA) as a hydrophilic block and poly(lauryl methacrylate) (PLMA) as a hydrophobic block were synthesized, which were then incorporated into the coating matrix poly(ε-caprolactone)-b-poly(dimethylsiloxane) (PCL–PDMS) for the fabrication of marine coatings. With the hydrolysis of PCL in water, the self-assembled amphiphilic copolymer may be exposed on coating surfaces with the PHEMA block extending to water. Experimental results proved that the hydration effects of hydroxyl groups in the PHEMA block enhanced the surface hydrophilicity and accelerated the weight loss rate. More importantly, the antibacterial rate of the coating against Escherichia coli and Staphylococcus aureus can reach 98.41 and 97.67%, respectively, in comparison with those of 55.36 and 51.97%, respectively, for the PCL–PDMS matrix. Additionally, the marine field tests elucidated that the amphiphilic polymers with longer PHEMA chains exhibit stronger antifouling capability in the marine environment. And the marine field tests evidenced that the developed PLMA-b-PHEMA incorporated coatings can withstand biofouling for up to 5 months.
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高影响因子
POLYMER
·
2026
·
IF 5.1
ZiYi Wu,Wenyu Jin,BaiZhang Zuo,WenZeng Guo,YeYao Guo,Junhee Lee,Seok-Jo Yang,Jiazi Gao,Meiling Quan,Yuan-Zhu Xin
Biodegradable vascular stents hold promise for addressing restenosis caused by permanent metal stents, yet they still face critical bottlenecks, including immune rejection-mediated inflammatory responses and insufficient mechanical properties. This study proposes a 3D-printed biodegradable vascular stent design strategy that integrates mechanical performance with anti-inflammatory and anti-rejection properties. A helical stent with additional connecting ribs was designed. Finite element simulation and mechanical testing were used to characterize the stent's mechanical properties. Cell proliferation and scratch assays were conducted to screen the optimal concentration of spermidine-loaded hydrogel, followed by pro-inflammatory/anti-inflammatory polarization of macrophages to evaluate spermidine's anti-inflammatory efficacy. Results demonstrate that the designed helical stent exhibits excellent mechanical properties. Spermidine released from the coating significantly enhances cell viability and effectively suppresses immune rejection. This study provides a feasible technical pathway and theoretical foundation for the biomodification and structural optimization of degradable vascular stents.
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