JOURNAL OF PHARMACY AND PHARMACOLOGY
·
2013
·Vol. 66
·期 4
·557-563
·
IF 2.033
Zhang Zhaoliang,Xu Lu,Chen Hao,Li Xingyi
ObjectivesAllograft rejection is the major cause of corneal graft failure. To inhibit corneal allograft rejection, rapamycin as a novel immunosuppressive agent has been discovered. However, the high water insolubility and low bioavailability of rapamycin has strongly hindered its application in the clinical setting. In this paper, we attempted to develop a novel rapamycin nano-formulation using poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC) nanoparticles as carrier by an emulsion evaporation method for potential application in corneal transplantation.MethodsThe solubility of rapamycin in the nano-formulation was determined and in-vitro release studies were performed. The developed rapamycin-loaded PCEC nanoparticles were further characterized by dynamic light scattering, transmission electron microscopy, X-ray diffraction and differential scanning calorimetery. Toxicity studies were performed in eye-related cell lines.Key findingsThe rapamycin in nano-formulation exhibited ∼103-fold increased solubility as compared with native rapamycin. According to the results of the in-vitro cytotoxicity assay, the developed PCEC nanoparticles did not exhibit any apparent cytotoxicity against various eye-related cell lines with PCEC nanoparticle concentrations in the range of 0.05–10 mg/ml. In-vitro release study showed that the release of rapamycin was sustained from PCEC nanoparticles over a period of 48 h.ConclusionsAll the results suggested that the developed rapamycin-loaded PCEC nanoparticles might be suitable for immunosuppression in corneal transplantation by instillation administration.
研究领域:
药剂学
·
眼科医学
·
生物材料学
✓ 已验证
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2018
·
IF 4.432
JinFeng Liao,YanPeng Jia,BeiYu Wang,Kun Shi,ZhiYong Qian
In our study, a hybrid alginate hydrogel cross-linked by calcium gluconate crystals deposited in poly(ε-caprolactone)-b-poly(ethylene glycol)-b-poly(ε-caprolactone) (PCL-PEG-PCL, abbreviated as PCEC) porous microspheres was developed for skin engineering. The diameter of microspheres was ∼212 μm, and the pore size was ∼8 μm. The PCEC porous microspheres supplied different functions in the hydrogel: (1) Calcium gluconate crystals were loaded in the inner pores of the microspheres, which can induce alginate hydrogel to cross-link in a few minutes once they were mixed. (2) The porous structure of the microspheres provided more anchor points for fibroblast attachment and growth, resulting in the enhancement of cell growth in the hybrid hydrogel. The PCEC microspheres/Alg hydrogel (MPs/Alg hydrogel) possessed excellent compatibility, because cell viability remained around 100% even at a concentration of 500 μg/mL. Meanwhile, the morphology of 3T3 and L929 cells attached on both PCEC porous microspheres and MPs/Alg hydrogel were confirmed by confocal laser spectrometry (CLSM). What’s more, MPs/Alg hydrogel promoted wound regeneration in a full-thickness skin defect model of rats. The mild inflammation reaction existed at the early stage of wound repair and gradually disappeared. These findings suggested that MPs/Alg hydrogel may possess great potential in the application of skin tissue engineering.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2017
·
IF 3.756
Fangyuan Guo,Dingjia Guo,Wei Zhang,Qinying Yan,Yan Yang,Weiyong Hong,Gensheng Yang
Biodegradable polymeric nanoparticles (NPs) have potential therapeutic applications; however, preparing NPs of a specific diameter and uniform size distribution is a challenge. In this work, we fabricated a microchannel system for the preparation of curcumin (Cur)-loaded NPs by the interfacial precipitation method, which rapidly and consistently generated stable NPs with a relatively smaller diameter, narrow size distribution, and higher drug-loading capacity and entrapment efficiency. Poly(ε-caprolactone)-poly(ethylene glycol)-poly (ε-caprolactone) triblock copolymers(PCEC) used as the carrier material was synthesized and characterized. Cur-loaded PCEC NPs had an average size of 167.2 nm with a zeta potential of − 29.23 mV, and showed a loading capacity and drug entrapment efficiency of 15.28% ± 0.23% and 96.11% ± 0.13%, respectively. Meanwhile, the NPs demonstrated good biocompatibility and bioavailability, efficient cellular uptake, and long circulation time and a possible liver targeting effect in vivo. These results indicate that the Cur-loaded PCEC NPs can be used as drug carriers in controlled delivery systems and other biomedical applications.
研究领域:
药剂学
·
生物材料学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2012
·
IF 5.479
Bei Feng,Hongbin Tu,Huihua Yuan,Hongju Peng,Yanzhong Zhang
In tissue engineering research, there has recently been considerable interest in using electrospun biomimetic nanofibers of hybrids, in particular, from natural and synthetic polymers for engineering different tissues. However, phase separation between a pair of much dissimilar polymers might give rise to detrimental influences on both the electrospinning process and the resultant fiber performance. A representative natural-synthetic hybrid of gelatin (GT) and polycaprolactone (PCL) (50:50) was employed to study the phase separation behavior in electrospinning of the GT/PCL composite fibers. Using trifluoroethanol (TFE) as the cosolvent of the two polymers, observation of visible sedimentation and flocculation from dynamic light scattering analysis of the GT/PCL/TFE mixture both showed that phase separation does occur in just a few hours. This consequently led to gradually deteriorated fiber morphologies (e.g., splash, fiber bonding, and varied fiber size) over time during electrospinning GT/PCL. Quantitative analysis also indicated that the ratio of GT to PCL in the resultant GT/PCL fibers was altered over time. To address the phase separation related issues, a tiny amount (<0.3%) of acetic acid was introduced to improve the miscibility, which enabled the originally turbid solution to become clear immediately and to be single-phase stable for more than 1 week. Nanofibers thus obtained also appeared to be thinner, smooth, and homogeneous with enhanced performance in wettability and mechanical properties. Given the versatility and widely uses of the electrospun GT/PCL and other similar natural-synthetic hybrid systems in constructing tissue-engineered scaffolds, this work may offer a facile and effective approach to achieve finer and compositionally homogeneous hybrid nanofibers for effective applications.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2023
·Vol. 34
·期 8
·2463-2470
·
IF 3.4
Dongxiang Chen,Yang Luo,Yan Song,Wencai Wang,Guo Liang Li
The development of a single polymer chain with the dual functions of self-healing and degradation is challenging. Herein, we report the synthesis of self-healing and degradable polymers (SDP) via the integration of hierarchical hydrogen bonding groups and degradable polycaprolactone (PCL) segments. The dynamic reconstruction of hierarchical hydrogen bonding endowed the as-prepared polymers with excellent self-healing properties. The degradation properties were regulated via the proportion of PCL in the soft segments. The incorporation of PCL segments resulted in better degradability and improved the tensile strength of the polymer from 9.2 to 28.3 MPa. The as-prepared polymers could recover their mechanical properties after self-healing. The temperature-dependent Fourier-transform infrared spectra (FT-IR) indicated that the hierarchical hydrogen bonding was capable of dissociation and re-association with a variation in the temperature. The as-synthesized sustainable polymers, which exhibit self-healing properties and degradability, are expected to be useful in future energy and environmental applications.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Asian Journal of Pharmaceutical Sciences
·
2018
·
IF 4.56
Yang Pan,Xiahui Wang,Zongning Yin
To achieve targeted thrombolysis, a targeted delivery system of lumbrokinase (LK) was constructed using RGDfk-conjugated hybrid micelles. Based on the specific affinity of RGDfk to glycoprotein complex of GPⅡb/Ⅲa expressed on the surface of membrane of activated platelet, LK loaded targeted micelles (LKTM) can be delivered to thrombus. The hybrid micelles were composed of polycaprolactone-block-poly (2-(dimethylamino) ethyl methacrylate) (PCL-PDMAEMA), methoxy polyethylene glycol-block- polycaprolactone (mPEG-PCL) and RGDfk conjugated polycaprolactone-block- polyethylene glycol (PCL-PEG-RGDfk). PCL-PDMAEMA was synthesized via ring open polymerization (ROP) and atom transfer radical polymerization (ATRP). PCL-PEG-RGDfk was synthesized via ROP and carbodiimide chemistry. The prepared LKTM was characterized by dynamic light scattering (DLS) and transmission electron microscope (TEM). Colloidal stability assay showed the prepared LKTM was stable. Biocompatibility assay was performed to determine the safe concentration range of polymer. The assay of fluorescent distribution in vivo demonstrated that LKTM can be efficiently delivered to thrombi in vivo . Thrombolysis in vivo indicated the thrombolytic potency of LKTM was optimal in all groups. Notably, the laboratory mice treated with LKTM exhibited a significantly shorter tail bleeding time compared to those treated with LK or LK-loaded micelles without RGDfk, which suggested that the targeted delivery of LK using RGDfk-conjugated hybrid micelles effectively reduced the bleeding risk.
研究领域:
药剂学
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Kun Jiao,Maolei Sun,Wenyuan Jia,Yun Liu,Shaoru Wang,Yuheng Yang,Zhihui Dai,Liping Liu,Zhiqiang Cheng,Guomin Liu,Yungang Luo
Electrospinning is a widely recognized method for producing Janus or core-shell nanofibers. In this study, nanofibrous membranes were fabricated through co-axial electrospinning utilizing polycaprolactone (PCL) and silk fibroin (SF) as the Janus shell, and taxifolin (TAX) and SF as the core. The resulting nanofibers had diameters of 816 ± 161 nm and core diameters of 73 ± 5 nm. The morphology and properties of the PCL-SF@SF/TAX nanofibers were subsequently analyzed. The results demonstrated that the nanofibrous membranes achieved physical and chemical characteristics potential for tissue engineering and drug delivery. Specifically, the membranes exhibited a Young's modulus of 9.64 ± 0.29 MPa, a water contact angle of 79.1 ± 1.3°, and a weight loss of 17.3 ± 1.0 % over a period of 28 days. The incorporation of TAX endowed the membranes with antibacterial properties, effectively combating Escherichia coli and Staphylococcus aureus . Furthermore, the membranes demonstrated antioxidant capabilities, with a DPPH radical scavenging efficiency of 38.5 ± 5.6 % and a Trolox-equivalent antioxidant capacity of 0.24 ± 0.01 mM. The release of the antioxidant was sustained over 28 days, following first-order release kinetics. The nanofibrous membranes, referred to as PSST, exhibit promising potential for use as biomaterials, characterized by their antibacterial activity, antioxidant and cytocompatibility.
研究领域:
生物材料学
·
组织工程学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Polymer Science
·
2019
·
IF 1.892
Wei Xiong,Yue-kun Shen,Peng Dong,Ying Xiao,Xiong-qing Huang,Wen-qi Huang,Lu Yang,Xia Feng
Sustained release of anesthesia has shown great promise in the treatment of chronic pain in patients. In this research, we used neutralized ropivacaine as an anesthesia and poly(ε-caprolactone) (PCL) with different architectures to systematically study how these architectures affect the release of ropivacaine. After optimizing the parameters of the preparation of microspheres, ropivacaine-loaded 1-PCL microspheres and 4-PCL microspheres were obtained. Fourier Transform infrared spectra (FT-IR) and X-ray diffraction spectra (XRD) confirmed that ropivacaine was encapsulated within the microsphere rather than inserted on the surface of the microsphere. Ropivacaine was found to be buried deeper in the 1-PCL microsphere than in the 4-PCL microsphere. In vitro release assay revealed that small crystalline grains interfered with ropivacaine release in 4-PCL microspheres during the initial release period, but then two kinds of microspheres showed a similar ropivacaine release rate. We basically proved that the architecture of PCL has a negligible effect on ropivacaine release. Cell proliferation test revealed that the release of products from the microspheres resulted in insignificant toxicity towards mammalian cells.
研究领域:
药剂学
·
材料科学
·
疼痛管理
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2017
·
IF 4.233
Rui Shi,Huan Geng,Min Gong,Jingjing Ye,Chengai Wu,Xiaohua Hu,Liqun Zhang
Trimethoxysilylpropyl octadecyldimethyl ammonium chloride (QAS), which forms facile bonds with hydroxyl groups , acts as a cationic antibacterial agent . In this work, QAS was introduced into a polycaprolactone (PCL)/gelatin hybrid in increasing concentrations to fabricate a long-acting and broad-spectrum antimicrobial micro/nanofiber membrane as a novel wound dressing. The physical interactions and chemical bonding between QAS/PCL and QAS/gelatin were demonstrated by infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS. Measured water contact angle between the PCL-gelatin/QAS (PG-Q) nanofiber membranes suggested a hydrophobic surface , which has been shown to aid in removal of wound dressings. The mechanical strength of the membranes was sufficient to meet the clinical requirements. Furthermore, the 15% QAS (PG-Q15) and 20% QAS (PG-Q20) formulated nanofiber membranes showed a considerable increase in their bacteriostatic activity towards Staphylococcus aureus (gram-positive) and Pseudomonas aeruginosa (gram-negative) bacteria, suggesting a broad-spectrum bactericidal effect by the PG-Q membranes. The PG-Q membranes with various QAS formulations demonstrated little cytotoxicity. Therefore, the long-acting and broad-spectrum antimicrobial electrospun PG-Q micro/nanofibers membrane demonstrate potential efficacy as an antibacterial wound dressing.
研究领域:
生物医学工程
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2022
·Vol. 14
·期 2
·283
·
IF 4.967
Wei Chen,Ping Zhu,Yating Chen,Yage Liu,Liping Du,Chunsheng Wu
Iodine has been widely used as an effective disinfectant with broad-spectrum antimicrobial potency. However, the application of iodine in an antibacterial polymer remains challenging due to its volatile nature and poor solubility. Herein, iodine immobilized UiO-66-NH2metal-organic framework (MOF) (UiO66@I2) with a high loading capacity was synthesized and used as an effective antibacterial additive for poly(ε-caprolactone) (PCL). An orthogonal design approach was used to achieve the optimal experiments’ conditions in iodine adsorption. UiO66@I2nanoparticles were added to the PCL matrix under ultrasonic vibration and evaporated the solvent to get a polymer membrane. The composites were characterized by SEM, XRD, FTIR, and static contact angle analysis. UiO-66-NH2nanoparticles have a high iodine loading capacity, up to 18 wt.%. The concentration of iodine is the most important factor in iodine adsorption. Adding 0.5 wt.% or 1.0 wt.% (equivalent iodine content) of UiO66@I2to the PCL matrix had no influence on the structure of PCL but reduces the static water angle. The PCL composites showed strong antibacterial activities againstStaphylococcus aureusandEscherichia coli. In contrast, the same content of free iodine/PCL composites had no antibacterial activity. The difference in the antibacterial performance was due to the different iodine contents in the polymer composites. It was found that MOF nanoparticles could retain most of the iodine during the sample preparation and storage, while there was few iodine left in the free iodine/PCL composites. This study offers a common and simple way to immobilize iodine and prepare antibacterial polymers with low antiseptic content that would reduce the influence of an additive on polymers’ physical properties.Keywords:UiO-66-NH2metal-organic framework;iodine;poly(ε-caprolactone);antibacterial
研究领域:
材料科学
·
生物学
·
医学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2020
·Vol. 20
·期 12
·7376-7384
·
IF 1.134
Yuan Chen,Ren Xin,Ye Huilin,Jin Shue,Zuo Yi,Li Jidong,Li Yubao
Mechanical and degradation properties are crucial factors of guided tissue/bone regeneration (GTR/GBR) membranes. In this work, a series of fibrous membranes with different ratios of polycaprolactone (PCL) and gelatin (Gel) were prepared (PCL:Gel = 1:9 (P1G9), 3:7 (P3G7), 5:5 (P5G5),
7:3 (P7G3), and 9:1 (P9G1)) by electrospinning, and their physicochemical properties and In Vitro degradation behaviors were systematically investigated. Mechanical tests showed that tensile strength was enhanced with the presence of Gel, and the tensile strength of the P9G1 membrane
reached nearly three times that of the pure PCL membrane. The degradation rate of the composite membranes could be adjusted by controlling the ratio of PCL and Gel; the higher the Gel content was, the faster the degradation of the PCL/Gel membrane. The higher PCL content favored maintaining
the fibrous structure of the electrospun membranes. These findings will be beneficial for designing PCL/Gel composite materials for biomedical applications.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2011
·
IF 2.546
Jiliang Wu,Chunyan Liao,Zheng Wang,Wenze Cheng,Nuo Zhou,Sheng Wang,Ying Wan
Chitosan–polycaprolactone (CH–PCL) copolymers with brush-like chain structures were synthesized. Some CH–PCLs with a PCL content less than 50 wt% were used to build glial cell line-derived neurotrophic factor (GDNF)-loaded microspheres via an emulsification method using genipin as a cross-linker. The resulting microspheres were spherical with a smooth surface and a diameter less than 40 μm; however, their loading efficiency could be higher than 80% if crosslinked properly. These microspheres had various swelling characteristics in lysozyme-containing PBS, which mainly depended on the PCL content, and they did not exhibit significant degradation up to 3 weeks. It is found that the PCL content in the CH–PCLs predominantly governed GDNF releases from some optimal microspheres and that the amount of cross-linker imposed limited impacts on the release behavior. Release kinetics revealed that GDNF release from microspheres was controlled by anomalous mechanisms involving Fickian diffusion and case-II transport. Some microspheres with an initial loading ratio of approximately 5-ng GDNF to 1.0-mg dry microsphere had sustained GDNF release upon addition of a simulant in vivo medium; an approximate linear rate of release was observed, which lasted over 4 weeks, was controllable and did not involve a significant burst release.
研究领域:
生物医学工程
·
药剂学
✓ 已验证
DOI
高影响因子
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