POLYMER COMPOSITES
·
2016
·
IF 2.004
Peng Wang,Pengzheng Liu,Fan Xu,Xiaoxia Fan,Huipin Yuan,Hong Li,Yonggang Yan
Ternary biocomposites of dicalcium phosphate (DCP), calcium sulfate (CS), and poly(amino acid) copolymer (PAA) could be used as a promising degradable biomimic bone substitute. Using an in vitro degradation model, we evaluated the influence of polymerization conditions, initial amino acids, and (DCP + CS)/PAA ratio on the degradation of the resulting materials. PAA degradation was affected by both the polymerization conditions and the initial amino acids, and the degradation of the DCP/CS/PAA composites was affected by the (DCP + CS)/PAA ratio. Using a certain initial amino acids (75% ε-aminohexanoic acid, 19% γ-aminobutanoic acid, 4% l -proline, and 2% l -lysine) and polymerization conditions (i.e., 210°C for 2.5 h) as the polymeric phase, (DCP + CS)/PAA biocomposites were prepared with 70%, 60%, and 40% PAA. All of the biocomposites were degradable, which resulted in the release of ions/amino acids and a slight pH fluctuation of 6.76 to 7.20. In addition, these biocomposites were biocompatible based on the proliferation of L929 but varied with the degradation rates. These results suggest the potential to control the degradation rates of (DCP + CS)/PAA biocomposites for bone regeneration. POLYM. COMPOS., 39:E122–E131, 2018. © 2016 Society of Plastics Engineers
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2020
·Vol. 39
·期 3
·327-336
·
IF 3.154
Shi Zhen,Wang Qi,Li Gui-Fei,Shou Yu-Feng,Zong Hong-Jie,Yan Shi-Feng,Zhang Kun-Xi,Yin Jing-Bo
Self-healing hydrogels with the shear-thinning property are novel injectable materials and are superior to traditional injectable hydrogels. The self-healing hydrogels based on 2-ureido-4[1 H ]-pyrimidinone (UPy) have recently received extensive attention due to their dynamic reversibility of UPy dimerization. However, generally, UPy-based self-healing hydrogels exhibit poor stability, cannot degrade in vivo and can hardly be excreted from the body, which considerably limit their bio-application. Here, using poly (l -glutamic acid) (PLGA) as biodegradable matrix, branching α-hydroxy- ω -amino poly(ethylene oxide) (HAPEO) as bridging molecule to introduce UPy, and ethyl acrylate polyethylene glycol (MAPEG) to introduce double bond, the hydrogel precursors (PMHU) are prepared. A library of the self-healing hydrogels has been achieved with well self-healable and shear-thinning properties. With the increase of MAPEG grafting ratio, the storage modulus of the self-healing hydrogels decreases. The self-healing hydrogels are stable in solution only for 6 h, hard to meet the requirements of tissue regeneration. Consequently, ultraviolet (UV) photo-crosslinking is involved to obtain the dual crosslinking hydrogels with enhanced mechanical properties and stability. When MAPEG grafting ratio is 35.5%, the dual crosslinking hydrogels can maintain the shape in phosphate-buffered saline solution (PBS) for at least 8 days. Loading with adipose-derived stem cell spheroids, the self-healing hydrogels are injected and self-heal to a whole, and then they are crosslinked in situ via UV-irradiation, obtaining the dual crosslinking hydrogels/cell spheroids complex with cell viability of 86.7%±6.0%, which demonstrates excellent injectability, subcutaneous gelatinization, and biocompatibility of hydrogels as cell carriers. The novel PMHU hydrogels crosslinked by quadruple hydrogen bonding and then dual photo-crosslinking of double bond are expected to be applied for minimal invasive surgery or therapies in tissue engineering.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Yanli Bao,Haiyang Yu,Yu Zhang,Li Chen
The photosensitizers (PS) play a vital role in photodynamic therapy (PDT), but the clinical therapeutic effect is limited by its low solubility, easy aggregation and lack of selective tumor uptake. Hence, some biocompatible materials such as poly(amino acid) have been chosen to deliver PS to solve these problems. In this study, we fabricated two kind of poly(amino acid)-based PS delivery systems by using poly ( L -glutamic acid) (PLG) as the backbone material to physically encapsulate (P(T)) and chemically conjugate PS (PT), respectively. Moreover, the anticancer effect of these two PS delivery systems had been comparatively investigated. In vitro experiments verified that the delivery system of PT exhibited more effective treatment effect than that of P(T) because PS was chemically conjugated with PLG in PT which could obviously avoid the π-π stacking effect of PS and induced the aggregation. The less aggregation avoiding the quenching effect could result in an enhanced generation ability of reactive oxygen species to improve PDT treatment effect. Contrarily, the treatment effect of P(T) was lower due to the rigid structure and hydrophobic π-π stacking effect, in which the physically encapsulated PS was easier to aggregate and quench. Hence, the way how to combine carrier with drug is significant for satisfied treatment effect.
研究领域:
生物医学工程
·
药学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2021
·Vol. 29
·期 1
·1-8
·
IF 3.097
Li Liying,Feng Jianhua,Shao Ningning,Shen Chen
A amphiphilic and biodegradable block copolymer is obtained by introducing hydrophilic polyethylene glycol segments into the segments of polyamino acids through copolymerization. And the block copolymers, poly(L-phenylalanine)-b-poly(ethylene glycol), are used to fabricate silica materials through Evaporation-Induced Self-Assembly method. In the synthesis procedure, anilino-methyl triethoxy silane is used as an intermedium, on the one hand it interacts with polypeptide-based copolymer through π-π interaction between the phenyl groups of phenylalanine segments and anilino-methyl triethoxy silane; on the other hand, anilino-methyl triethoxy silane can co-condense with tetraethoxylsilane through hydrolysis process. The prepared silica possesses supermicropores and it is proposed that the supermicropores are templated by the polypeptide segments. It is proved that both polypeptide-based block copolymer and anilino-methyl triethoxy silane play important roles in the formation of mesoscale short-range-order and hierarchical structure. When the phenylalanine segment of block copolymer is longer, it can induce a well-defined shuttle-like morphology of polymer/silica hybrid particles.
研究领域:
材料科学
·
生物化学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2015
·
IF 4.152
Na Liu,Bingqiang Li,Chu Gong,Yuan Liu,Yanming Wang,Guolin Wu
A pH- and thermo-responsive poly(amino acid)-based amphiphilic copolymer was developed, functioning as a tumour targeting drug delivery system with good biocompatibility and biodegradability. To provide multi-stimuli sensitivity characteristics to the poly(amino acid)s, the polyaspartamide scaffold has been functionalized with N , N -diisopropylamide groups via aminolysis reaction of polysuccinimide. PEG chains have also been chemically grafted to the poly(amino acid) backbone through acid-labile hydrazone linkages, providing a removable shield for the poly(amino acid) based nanoparticles. Furthermore, doxorubicin was chemically linked to the copolymer chain via hydrazone bonds, acting as the hydrophobic moiety to drive the polymeric self-assembly. Free doxorubicin molecules could be encapsulated into the self-assembled nanoparticles via hydrophobic interactions and molecular π–π stacking. The results obtained show that the drug release can be triggered by the temperature with a significantly increased release being observed under acidic conditions. The cytotoxicity behaviour of the copolymers and drug-loaded nanoparticles was investigated in vitro at varying pH values and different temperatures. In doing so, superior characteristics concerning compatibility and anti-cancer activity could be observed.
研究领域:
生物材料学
·
药剂学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2024
·Vol. 16
·期 21
·10448-10457
·
IF 5.8
Feng Yang,Shiwen Gong,Die Hu,Lihua Chen,Wenyuan Wang,Bo Cheng,Jing Yang,Binbin Li,Xinyu Wang
With continuous advances in medical technology, non-invasive embolization has emerged as a minimally invasive treatment, offering new possibilities in cancer therapy. Fluorescent labeling can achieve visualization of therapeutic agents in vivo, providing technical support for precise treatment. This paper introduces a novel in situ non-invasive embolization composite material, Au NPs@(mPEG-PLGTs), created through the electrostatic combination of L-cysteine-modified gold nanoparticles (Au NPs) and methoxy polyethylene glycol amine-poly[(L-glutamic acid)-(L-tyrosine)] (mPEG-PLGTs). Experiments were undertaken to confirm the biocompatibility, degradability, stability and performance of this tumor therapy. The research results demonstrated a reduction in tumor size as early as the fifth day after the initial injection, with a significant 90% shrinkage in tumor volume observed after a 20-day treatment cycle, successfully inhibiting tumor growth and exhibiting excellent anti-tumor effects. Utilizing near-infrared in vivo imaging, Au NPs@(mPEG-PLGTs) displayed effective fluorescence tracking within the bodies of nude BALB-c mice. This study provides a novel direction for the further development and innovation of in situ non-invasive embolization in the field, highlighting its potential for rapid, significant therapeutic effects with minimal invasiveness and enhanced safety.
研究领域:
生物医学工程
·
癌症治疗
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science of Advanced Materials
·
2017
·Vol. 9
·期 10
·1713-1723
·
IF 1.671
Li Mengmeng,Chen Yongchao,Yin Li,Wang Haiyan,Cao Yanping,Wei Zhihao,Wang Yanfei,Yuan Qi,Pu Xiaohui,Zong Lanlan,Duan Shaofeng
To manufacture a stable paclitaxel nanosuspension (PTX-CP-Nanos), poly(L-phenyl-alanine)-b-poly(L-aspartic acid) (PPA-PAA), a multifunctional stabilizer, was synthesized via NCA-based ring-opening copolymerization. PTX-CP-Nanos were manufactured by a microprecipitation-high pressure
homogenization method. X-ray powder diffraction (XRD) and differential scanning calorimetry (DSC) results indicated that the PTX in PTX-CP-Nanos existed in amorphous state. Particle size and zeta potential of the PTX-CP-Nanos were 226.8 ± 2.1 nm and –28.4 ± 0.1 mV, respectively,
as indicated by dynamic light scattering method. Transmission electron microscopy (TEM) revealed that the morphology of PTX-CP-Nanos were of rod-like crystals. In addition, it was found that the structure of PTX-CP-Nanos altered to micelles by TEM during dissolution. The dissolution rate of
PTX-CP-Nanos was significantly increased compared with the crude crystals but obviously lower than PTX injection, which could be ascribed to the particle size reduction of PTX nanoparticles and the conversion of PTX-CP-Nanos into micelles in the later stage of the dissolution test. The tests
of storage stability, dilution stability and plasma stability both showed that PTX-CP-Nanos were significantly more stable than PTX-Nanos stabilized by other traditional surfactants and polymers. The acute toxicity tests demonstrated that PTX-CP-Nanos also had a high systemic safety.
研究领域:
药剂学
·
生物材料学
·
药物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Dazhuang Xu,Meiying Liu,Qiang Huang,Junyu Chen,Hongye Huang,Fengjie Deng,Jianwen Tian,Yuanqing Wen,Xiaoyong Zhang,Yen Wei
Recently, fullerene (C 60 ) and its derivatives have been widely explored for many applications owing to their enriched physical and chemical properties. Specifically, the synthesis and biomedical applications of fluorescent C 60 have been extensively investigated previously. However, the preparation of polymer-functionalized fluorescent C 60 has not been reported thus far. In this study, water-dispersible fluorescent C 60 polymer composites were successfully synthesized through the combination of the thiol-ene click reaction and subsequent ring-opening polymerization. First, 2-aminoethanethiol was introduced on the surface of C 60 by the thiol-ene click reaction. The surface of amino group-functionalized C 60 (C 60 -NH 2 ) was further modified with poly(amino acid)s via ring-open polymerization of GluEG N-carboxyanhydrides (NCAs). The morphology, functional groups, optical properties and biocompatibility were examined by a number of characterization equipment and assays in detail. We demonstrated that the resultant fluorescent C 60 poly(amino acid) (C 60 -GluEG) composites have a small size (about 5 nm), high water dispersibility, intense fluorescence and high photostability. Cell viability results implied that the C 60 -GluEG composites possess low cytotoxicity. Moreover, these C 60 -GluEG composites can easily penetrate into live cells, indicating their great potential for biological imaging applications.
研究领域:
材料科学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2020
·Vol. 12
·期 8
·5227-5232
·
IF 6.895
Ting Hou,Shanshan Yu,Minfeng Zhou,Min Wu,Jie Liu,Xiaoli Zheng,Jinxing Li,Joseph Wang,Xiaolei Wang
The increasing extent of heavy metal pollution all over the world has resulted in many serious environmental and public health problems. To solve these problems, effective technologies for water treatment are urgently needed. Recent efforts have focused on the development of self-driven micro/nanomotors for eliminating inorganic and organic pollutants in an aqueous system. These synthetic micro/nanomotors can increase mass transfer through the transportation of reactive species, leading to higher decontamination rates. Here, we report a surface-tunable poly(amino acid) (PAA)-based micromotor. The property of the outer layer can be adjusted by changing the type and proportion of amino acids according to real requirements. Three kinds of micromotors are fabricated, which consist of a microtube composed of PAAs (i.e., polyaspartic acid (PAsp), polycysteine (PCys) or a copolymer of both (PAsp-Cys)), a thin Ni intermediate layer, and a Pt inner layer. Due to the presence of various side-chain functional groups (e.g., amino, carboxyl, and sulfhydryl) on the surface of the poly(amino acid)s, these micromotors can be used as effective scavengers for the removal of heavy metals (i.e., Cd2+, Pb2+ and methylmercury). Compared with PAsp and PCys micromotors, the PAsp-Cys micromotor shows good acid resistance and can simultaneously adsorb various kinds of heavy metals with high removal efficiency. The outer layer of the surface-tunable micromotor has good biocompatibility and adsorption efficiency, which holds considerable promise for environmental and biomedical applications.
研究领域:
环境科学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Dedai Lu,Yuanyuan Jin,Xiangya Wang,Liyuan Xie,Qianqian Liu,Yamin Chen,Hui Wang,Ziqiang Lei
Due to the uncontrollable anticoagulant activity and limited source, Heparin, which is commonly used in clinical anticoagulation therapies, faces the risk of spontaneous bleeding and thrombocytopenia. Herein, a series of anionic poly(amino acid) s poly ( l -Serine- ran -L-Glutamic acid- ran -L-Cysteine-SO 3 ) (PSEC-SO 3 ) were prepared by the controlled Ring Opening Polymerization (ROP) of N-Carboxyanhydrides (NCAs). The anticoagulant activities of PSEC-SO 3 can be regulated by simply adjusting the feeding ratio of monomers. In vitro tests show that these polypeptides can effectively prolong the Activated Partical Thromboplastin Time (APTT) and inhibit Factor IIa and Factor Xa, but has no significant effect on Prothrombin Time (PT) and Thrombin Time (TT), which indicates that PSEC-SO 3 mainly act on the intrinsic pathway. In summary, the activity-tunable heparin-like polypeptides are expected to have good application prospects in the anticoagulant field.
研究领域:
生物化学
·
材料科学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Zeping Yu,Fei Xing,Jianshu Li,Wenli Zhang,Hong Duan,Jian Li,Yan Xiong
Given the limited blood supply that results in the meniscus's poor self-healing ability, meniscal tissue engineering (MTE) scaffolds have emerged as an effective strategy for the treatment of meniscal injuries. However, the efficacy of mesenchymal stem cells (MSCs) is limited in the inflammatory state caused by chronic injuries, making it challenging for existing meniscal implants to promote meniscal regeneration and delay articular cartilage degeneration in an inflammatory environment. This study introduces a method to incorporate phosphoester (PE) units into the main chain of poly (amino acids) (PAAs) through ring-opening copolymerization, subsequently creating a copolymer with graphene oxide (GO) to yield phosphoester-modified poly (amino acids)-graphene oxide (P-P-GO). PCL/P-P-GO scaffolds were fabricated by blending P-P-GO with polycaprolactone (PCL) and employing 3D printing technology. The PAAs of P-P-GO scaffold provide a nutritious environment essential for MSC proliferation, migration, and differentiation, while the PE enhance the cell adhesion properties, with further improvements by GO. In vitro experiments have shown that the PCL/P-P-GO scaffold upregulates the expression of chondrogenic-specific genes and increase the production of collagen and glycosaminoglycans. Additionally, γ-aminobutyric acid of the scaffolds can suppress the secretion of inflammatory factors, induce M2 polarization of macrophages, and promote angiogenesis. In vivo studies using rabbit meniscectomy model have demonstrated that PCL/P-P-GO scaffolds can promote meniscal tissue regeneration and delay cartilage degeneration. After six months of implantation, the regenerated meniscus exhibited a structure and composition resembling natural meniscus, and the progression of osteoarthritis was significantly slowed. Compared to traditional cell-loaded scaffold strategy, PCL/P-P-GO scaffolds are easy to produce, cost-effective, and hold broad translational potential for the clinical treatment of meniscal injuries.
研究领域:
组织工程学
·
生物材料科学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2016
·
IF 1.733
Peng Wang,Pengzheng Liu,Haitao Peng,Xiaoman Luo,Huipin Yuan,Juncai Zhang,Yonggang Yan
In vitro cytocompatibility of ternary biocomposite of dicalcium phosphate (DCP) and calcium sulfate (CS) containing 40 wt% poly (amino acid) (PAA) was evaluated using L929 fibroblasts and MG-63 osteoblast-like cells. Thereafter, the biocompatibility of biocomposite in vivo was investigated using an implantation in muscle and bone model. In vitro L929 and MG-63 cell culture experiments showed that the composite and PAA polymer were noncytotoxic and allowed cells to adhere and proliferate. The scanning electron microscope (SEM) confirmed that two kinds of cells maintained their phenotype on all of samples surfaces. Moreover, the DCP/CS/PAA composite showed higher cellular viability than that of PAA; meanwhile, the cell proliferation and ALP activity were much higher when DCP/CS had added into PAA. After implanted in muscle of rabbits for 12 weeks, the histological evaluation indicated that the composite exhibited excellent biocompatibility and no inflammatory responses were found. When implanted into bone defects of femoral condyle of rabbits, the composite was combined directly with the host bone tissue without fibrous capsule tissue, which shown good biocompatibility and osteoconductivity. Thus, this novel composite may have potential application in the clinical setting.
研究领域:
骨科工程
·
生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）