ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Lichan Chen,Xiaoting Zeng,Anirban Dandapat,Yuwu Chi,Donghwan Kim
Proteases and nucleases are enzymes heavily involved in many important biological processes, such as cancer initiation, progression, and metastasis; hence, they are indicative of potential diagnostic biomarkers. Here, we demonstrate a new label free and sensitive electrochemiluminescent (ECL) sensing strategy for protease and nuclease assays that utilize target-triggered desorption of programmable polyelectrolyte films assembled on graphite-like carbon nitride (g-C3N4) film to regulate the diffusion flux of a coreactant. Furthermore, we have built Boolean logic gates OR and AND into the polyelectrolyte films, capable of simultaneously sensing proteases and nucleases in a complicated system by breaking it into simple functions. The developed intelligent permeability controlled enzyme sensor may prove valuable in future medical diagnostics.
研究领域:
生物医学工程
·
分析化学
·
分子诊断学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 32
·期 8
·2107598
·
IF 18.808
Da Zhang,Qing-Shuang Li,Zi-Xuan Liang,Xia-Chao Chen,Junran Hao,Juming Yao,Chun-Xin Lu,Yahong Zhou,Lei Jiang
Although many methods have been developed to create porous structures within polyelectrolyte films, it remains a challenge to conveniently define the spatial distribution of these structures. Herein, a simple, universal strategy with a high flexibility in programming porous regions throughout polyelectrolyte films is reported. This strategy is performed with the aid of laser scanning and the emergence of porous regions precisely tails after the moving path of laser beams. Quite different from previous strategies, the pore-forming mechanism relies on laser-triggered generation of gas to foam the films, which can be regulated by the content of photo-sensitive groups, the intensity of laser beams, and the supramolecular interactions between polyelectrolyte chains. A prerequisite to succeed the foaming of polyelectrolyte films is to irradiate them under water, where the mobility of polyelectrolytes is activated to accommodate the pore formation within the films. Although many pore-forming mechanisms involve aqueous systems, the porous structures here can be well preserved during drying procedures, which is hardly realized for conventional methods due to the formation of capillary pressure. The resulting polyelectrolyte films with patterned porosity can be quite useful in many fields, including but not limited to information display, surface wettability, and cell behavior manipulation.
研究领域:
材料科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
Science of Advanced Materials
·
2016
·Vol. 8
·期 8
·1547-1552
·
IF 1.812
Tao Gang,Wang Yejing,Liu Lina,Chang Huaipu,Zhao Ping,He Huawei
Sericin has shown a great potential as a biomaterial in biomedical application due to its good hydrophilicity, reactivity and biodegradability. To solve the adherence and growth of microorganisms on sericin gel, here we prepared a polyelectrolyte film with interactive arrangement of
opposite charges poly(dimethyldiallylammonium chloride) (PDDA) and poly(acrylic acid) (PAA) coated on the surface of sericin gel via electrostatic interaction. AgNPs was then synthesized with the assistance of UV in situ on the polyelectrolyte film to endow the antibacterial
activity of sericin gel. SEM revealed the polyelectrolyte film could effectively facilitate the high density growth of AgNPs as a 3-D matrix and the modification of polyelectrolyte and AgNPs did not alter the porous characteristics of sericin gel. XRD studies suggested AgNPs synthesized on
the polyelectrolytes film coated sericin gel had good crystal structure. DSC analysis showed AgNPs could tightly bind with the polyelectrolytes film and sericin gel, and the binding may improve the melting temperature of AgNPs. Both bacterial inhibition zone and growth curve assay demonstrated
the sericin gel coated with AgNPs-polyelectrolytes film had an excellent and long-lasting antibacterial activity. This novel sericin gel has shown great potentials in biomedical application such as wound healing.
研究领域:
生物医学材料
·
纳米技术
·
材料科学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Wei-Pin Huang,Xia-Chao Chen,Mi Hu,Jing Wang,Hong-Lin Qian,Deng-Feng Hu,Rui-Lin Dong,Song-Yi Xu,Ke-Feng Ren,Jian Ji
Inspired by nature, many functional surfaces have been developed with special structures in biology, chemistry, and materials. Many research studies have been focused on the preparation of surfaces with static structure. Achieving dynamical manipulation of surface structure is desired but still a great challenge. Herein, a polyelectrolyte film capable of regional and reversible changes in the microporous structure is presented. Our proposal is based on the combination of azobenzene (Azo) π–π stacking and electrostatic interaction, which could be affected respectively by ultraviolet (UV) irradiation and water plasticization, to tune the mobility of polyelectrolyte chains. The porous patterns can be obtained after regional ultraviolet irradiation and acid treatment. Owing to the reversibility of Azo π–π stacking and electrostatic interaction, the patterns can be repeatedly created and erased in the polyelectrolyte film made by layer-by-layer (LbL) self-assembly of poly(ethyleneimine)-azo and poly(acrylic acid). Furthermore, through two rounds of porous pattern formation and erasure, different functional species can be loaded separately and confined regionally within the film, showing potential applications in the functional surface. This work highlights the coordination of two noncovalent interactions in thin films for regional and reversible controlling its structure, opening a window for more in-depth development of functional surfaces.
研究领域:
材料科学
·
表面化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Zhi-Hui Xie,Wenxi Zhang,Yanqiu Li,Qiwen Yong,Liang Wu,Xiaoqiang Fan,Chuan-Jian Zhong
Layered double hydroxide (LDH) layers have emerged as a promising class of materials in preparing functional coatings due to their specific anion exchangeability. However, most LDH-based films exhibit micro- and nanoscale pores that are not conducive enough to block corrosive media penetration. This report demonstrates a corrosion-resistant LDH-based composite coating with an 8-hydroxyquinoline (8HQ)-sandwiched polyelectrolyte outer film. This polyelectrolyte film is flat and dense, enabling an effective pore-sealing of the LDH layer on the magnesium alloy and preventing the dissolution and diffusion of inhibitor into the bulk solution. This strategy is shown to enhance the corrosion protection performance significantly. Electrochemical measurements, including the scanning vibrating electrode technique (SVET), confirm that the as-prepared composite coating exhibits very high charge transfer resistance (∼90 MΩ·cm2), extremely low corrosion current density (<1.0 nA cm–2), and superior self-healing capability. The high-performance corrosion protection is attributed to a combination of the ion-exchange capability of the LDH phase, corrosion inhibition of the 8HQ inhibitor, intrinsic properties and sealing effect of the polyelectrolyte, and decreased hydrophilicity. These findings provide insight into highly efficient sealing for porous layers on metal surfaces to achieve coating with long-term corrosion protection and self-healing capability.
研究领域:
材料科学
·
腐蚀与防护
·
表面工程
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产品关联
DOI
高影响因子
CHEMISTRY OF MATERIALS
·
2019
·
IF 10.159
Wei-Pin Huang,Xiachao Chen,Mi Hu,Deng-Feng Hu,Jing Wang,He-Yang Li,Ke-Feng Ren,Jian Ji
Patterned slippery surfaces based on the technique of slippery liquid-infused porous surface (SLIPS) have broad technological applications in biofouling, liquid handling, biomolecule collection, and droplet microarrays. However, methods to generate patterned SLIPS with flexibility and practical potential remain a technical roadblock. Here, we report an approach for generating patterned SLIPS, and by utilizing which, as a proof-of-technique application, the droplet microarrays have been presented. Our approach is based on a photocross-linkable polyelectrolyte poly(ethyleneimine)/azide-poly(acrylic acid) (PEI/PAA-N3) film, whose surface structures can be dynamically controlled. We have prepared the surface-patterned PEI/PAA-N3 film containing microstructured and flat regions. We then demonstrate that, because of much higher lubricant retaining capability of the microstructured regions, a patterned SLIPS can be simply generated through infusing the lubricant onto the surface and applying a shear spinning process. This work highlights the importance of the dynamics of surface structures to functional materials, showing broad potential applications.
研究领域:
材料科学
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生物技术
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2015
·
IF 5.75
He Zhang,Hao Chang,Li-mei Wang,Ke-feng Ren,M. Cristina L. Martins,Mário A. Barbosa,Jian Ji
Endothelial-to-mesenchymal transition (EndMT), during which endothelial cells (ECs) transdifferentiate into mesenchymal phenotype, plays a key role in the development of vascular implant complications such as endothelium dysfunction and in-stent restenosis. Substrate stiffness has been confirmed as a key factor to influence EC behaviors; however, so far, the relationship between substrate stiffness and EndMT has been rarely studied. Here, ECs were cultured on the (poly(l-lysine)/hyaluronate acid) (PLL/HA) multilayer films with controlled stiffness for 2 weeks, and their EndMT behaviors were studied. We demonstrated that ECs lost their markers (vWf and CD31) in a stiffness-dependent manner even without supplement of growth factors, and the softer film favored the maintaining of EC phenotype. Further, induced by transforming growth factor β1 (TGF-β1), ECs underwent EndMT, as characterized by losing their typical cobblestone morphology and markers and gaining smooth muscle cell markers (α-smooth muscle actin and calponin). Interestingly, stronger EndMT was observed when ECs were cultured on the stiffer film. Collectively, our findings suggest that substrate stiffness has significant effects on EndMT, and a softer substrate is beneficial to ECs by keeping their phenotype and inhibiting EndMT, which presents a new strategy for surface design of vascular implant materials.
研究领域:
生物材料
·
细胞生物学
·
组织工程
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
·
2021
·Vol. 8
·期 15
·2100402
·
IF 16.806
Wei-Pin Huang,Hong-Lin Qian,Jing Wang,Ke-Feng Ren,Jian Ji
Periodic porous structures have been introduced into functional films to meet the requirements of various applications. Though many approaches have been developed to generate desired structures in polymeric films, few of them can effectively and dynamically achieve periodic porous structures. Here, a facile way is proposed to introduce periodic stratified porous structures into polyelectrolyte films. A photo-crosslinkable polyelectrolyte film of poly(ethylenimine) (PEI) and photoreactive poly(acrylic acid) derivative (PAA-N 3 ) is prepared by layer-by-layer (LbL) self-assembly. Stratified crosslinking of the PEI/PAA-N 3 film is generated basing on standing-wave optics. The periodic stratified porous structure is constructed by forming pores in noncrosslinked regions in the film. Thanks to the dynamic mobility of polyelectrolytes, this structural controlment can be repeated several times. The size of pores corresponding to the layer spacing of the film contributes to the structural colors. Furthermore, structural color patterns are fabricated in the film by selective photo-crosslinking using photomasks. Although the large-scale structural controlment in thick (micron-scale and above) films needs to be explored further, this work highlights the periodic structural controlment in polymeric films and thus presents an approach for application potentials in sensor, detection, and ink-free printing.
研究领域:
材料科学
·
光学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2019
·Vol. 15
·期 9
·1804867
·
IF 10.856
Xia-Chao Chen,Wei-Pin Huang,Mi Hu,Ke-Feng Ren,Jian Ji
Although many approaches have been developed to encapsulate functional species into polyelectrolyte films, few of them can effectively control the final distribution of these ones. Herein, a facile strategy is proposed to spatially control the encapsulation of guest species by locally regulating the structural transformation of polyelectrolyte films. Patterned porosity is created within a film by cross-linking it selectively and then immersing it in an acidic solution. These porous regions can exhibit significantly different properties from other regions, including the ability to wick solution, a greater retention of guest species, and the capability of structural transformation. After loading guest species, the porous structures can be eliminated at saturated humidity to encapsulate the guest species into the film, leading to their patterned distribution across the film. Based on this method, various guest species, ranging from fluorescent dyes to nanoparticles, can be locally encapsulated into polyelectrolyte film, forming distinct patterns of arbitrary shapes and sizes and thus paving the way for further applications.
研究领域:
材料科学
·
化学工程
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Jiadi Ying,Yuqing Lin,Yiren Zhang,Yan Jin,Hideto Matsuyama,Jianguo Yu
The development of cation exchange membranes (CEMs) with high monovalent/divalent cation selectivity has great potential for enhanced salt separation for wastewater reclamation, and layer-by-layer (LbL) self-assembly of polyelectrolyte films with high monovalent selectivities among cations has expanded the applications of such membranes. However, the structure–property relationship for constructing multilayered polyelectrolyte films with enhanced separation performance remains poorly understood. This study reports a novel CEM engineered by coating a [polyethyleneimine/polystyrene sulfonate (PEI/PSS)] n alternate electro-deposition multilayer on a polyacrylate substrate membrane. The newly developed CEM exhibited a monovalent selectivity of 4.59 for Li + /Mg 2+ with an optimal permeation of 0.16 mmol·cm −2 ·h −1 for Li + , demonstrating a competitive separation property compared to state-of-the-art permselective membranes. A detailed quantitative investigation provides the underlying insights into the monovalent selectivity for each polyelectrolyte layer. The results indicate negligible interface impedance toward monovalent cations but strengthened repulsion against divalent cations, thereby promoting a quick passage for monovalent cations while imposing temporal retardation on divalent cations during the electrodialysis (ED) process. Therefore, this study elucidates the characteristic reason for the enhanced monovalent ion permselectivity of LbL polyelectrolyte membranes in electro-driven separation processes.
研究领域:
材料科学
·
化学工程
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）