MATERIALS LETTERS
·
2016
·
IF 2.437
Huan Zhou,Shiqin Kong,Zhiwei Liu,Yan Pan,Yang Liu,Mingzhi Luo,Linhong Deng
Calcium polyphosphate (CPP) incorporated calcium alginate (CA) composite beads were prepared via adding sodium polyphosphate (STPP) contained sodium alginate (SA) solution dropwise to calcium chloride (CaCl 2 ) solution. In the process Ca 2+ ion may attach to the -COO − in SA because of electrostatic attraction. Additionally, there is an interaction of PP to Ca 2+ to generate CPP, inducing intermolecular crosslinkages between CA and CPP. The rotational rheometer showed STPP addition had no significant impacts on the rheological properties of SA solution. While a combination of SEM, AFM, FTIR and swelling behavior study demonstrated CPP was successfully formed within the gel network of CA gel beads and played an active role in restricting the movability of the alginate polymer chains. The in vitro study implied CPP can promote the expression of ALP , Col-I, OCN and OPN of osteoblasts.
研究领域:
材料科学
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生物医学工程
·
细胞生物学
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DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2021
·
IF 3.493
Caixia Cui,Mengyuan Kong,Yihan Wang,Chenyan Zhou,Hong Ming
Polyphosphate kinase (PPK) is important for industrial processes involving ATP regeneration. While a variety of methods have been reported for regenerating ATP from ADP, few have explored enzyme catalyzed ATP regeneration from cheaper and stable AMP. In this work, PPKs from different sources were expressed and their catalytic activity were tested at different reaction temperatures, reaction pH and with different polyphosphate (polyP n ) types. The ATP regeneration system for glutathione (GSH) synthesis was established using a single PPK capable of phosphorylating AMP to synthesize ATP from AMP and short chain polyP n . GSH yield was obtained using adenosine mono-, di- and triphosphates, which confirmed the flexibility of our constructed ATP regeneration system coupled with GSH synthesis via bifunctional GSH synthase. Finally, optimization of the GSH synthesis yielded conversion value above 80 %. Overall, these results illustrate that PPK is suitable for a broader range of substrates than previously expected, and has great untapped potential for applications involving ATP regeneration.
研究领域:
生物化学
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生物技术
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工业生物处理
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产品关联
DOI
PubMed
高影响因子
POLYMER
·
2016
·
IF 3.586
Jian Jing,Yan Zhang,Xinlei Tang,Yang Zhou,Xiaonan Li,Baljinder K. Kandola,Zhengping Fang
In this study, a facile approach to fabricate a novel bio-based hybrid (BBH) with core/shell structure is presented by taking advantage of plant-derived resources. The BBH developed herein comprises of an inorganic core of ammonium polyphosphate (APP), covered with an organic shell that is constructed via layer-by-layer assembly of a novel bio-based polyelectrolyte (BPE) and polyethylenimine (PEI). BBH can simultaneously enhance the flame retardancy and toughness of polylactic acid (PLA) with very high efficiency. The flame retardant PLA composite can pass UL94 V0 rating at the BBH loading content of 10 wt%. The efficient flame retardant performance is due to its effectiveness in both gas and condensed phases. More importantly, the flame retardant PLA composite containing 10 wt% BBH exhibits ductile fracture with an elongation at break of 27.3%, which is much higher than that of neat PLA (8%). The debonding and plastic void deformation of the PLA matrix around the BBH are responsible for the enhanced toughness. This work provides important hints for devising highly efficient multifunctional flame retardant and broadening the application field of renewable resources.
研究领域:
材料科学
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聚合物化学
·
生物质利用
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产品关联
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2017
·Vol. 101
·期 13
·5325-5332
·
IF 3.42
Cao Hao,Nie Kaili,Li Chengcheng,Xu Haijun,Wang Fang,Tan Tianwei,Liu Luo
Adenosine-5′-triphosphate (ATP) is the energy equivalent of the living system. Polyphosphate (polyP) is the ancient energy storage equivalent of organisms. Polyphosphate kinases (PPKs) catalyze the polyP formation or ATP formation, to store energy or to regenerate ATP, respectively. However, most PPKs are active only in the presence of long polyPs, which are more difficult and more expensive to generate than the short polyPs. We investigated the PPK preference towards polyPs by site-directed mutagenesis and computational simulation, to understand the mechanism and further design enzymes for effective ATP regeneration using short polyPs for in vitro cascade reactions, which are highly desired for research and applications. The results suggest that the short polyPs inhibit PPK by blocking the ADP-binding pocket. Structural comparison between PPK ( Corynebacterium glutamicum ) and PPK ( Sinorhizobium meliloti ) indicates that three amino acid residues, i.e., lysine, glutamate, and threonine, are involved in the activity towards short polyP by fixing the adenosine group of ADP in between the subunits of the dimer, while the terminal phosphate group of ADP still offers an active site, which presents a binding pocket for ADP. A proposed triple mutant PPK (SMc02148-KET) demonstrates significant activity towards short polyP to form ATP from ADP. The obtained high glutathione titer (38.79 mM) and glucose-6-phosphate titer (87.35 mM) in cascade reactions with ATP regeneration using the triple mutant PPK (SMc02148-KET) reveal that the tailored PPK establishes the effective ATP regeneration system for ATP-dependent reactions.
研究领域:
生物化学
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酶工程
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合成生物学
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DOI
PubMed
高影响因子
Bioresources and Bioprocessing
·
2021
·Vol. 8
·期 1
·1-10
·
IF 4.578
Sun Chuanqi,Li Zonglin,Ning Xiao,Xu Wentian,Li Zhimin
Adenosine triphosphate (ATP) acts as a crucial energy currency in vivo , and it is a widely used energy and/or phosphate donor for enzyme-catalyzed reactions in vitro. In this study, we established an in vitro multi-enzyme cascade system for ATP production. Using adenosine and inorganic polyphosphate (polyP) as key substrates, we combined adenosine kinase and two functionally distinct polyphosphate kinases (PPKs) in a one-pot reaction to achieve chain-like ATP regeneration and production. Several sources of PPK were screened and characterized, and two suitable PPKs were selected to achieve high rates of ATP production. Among these, Sulfurovum lithotrophicum PPK (SlPPK) exhibited excellent activity over a wide pH range (pH 4.0–9.0) and synthesized ATP from ADP using short-chain polyP. Furthermore, it had a half-life > 155.6 h at 45 °C. After optimizing the reaction conditions, we finally carried out the coupling-catalyzed reaction with different initial adenosine concentrations of 10, 20, and 30 mM. The highest yields of ATP were 76.0, 70.5, and 61.3%, respectively. Graphical Abstract
研究领域:
生物化学
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生物技术
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酶学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Wenju Zhu,Shuixia Liu,Huili Zhang,Dongxiang Li,Xiaohan Zhang,Wei Peng,Shuolong Wang,Shuang Xu,Chunming Zheng,Bowen Cheng
The durability and flame retardancy of cotton fabrics have been the focus of long-term research. In this paper, a method for preparing flame retardants through the direct modification of biomass was proposed, and the durable flame retardant of homologous cottonseed meal modified biomass flame retardants for cotton fabrics was achieved through biomass composition analysis and modeling. In this study, a cottonseed meal-phosphoric acid-boric acid synergistic bio-based flame retardant (CPB) was synthesized and characterized. The results of XPS and scanning electron microscope (SEM) showed that DC-CPB1 was mainly grafted on the superficial layer and surface of the fabric, and DC-CPB2 was mainly grafted on the surface of cotton fiber. In the vertical flammability test (VFT) and limiting oxygen index test (LOI), the LOI value of cotton fabric after 50 cycles of washing can reach up to 33.5 %, and the carbon damage length was reduced from 300 mm to a minimum of 69 mm, showing excellent flame-retardant performance and self-extinguishing ability. In the cone calorimetry test, the peak heat release rate (PHRR) was significantly reduced by 82.49 %, and the results of thermogravimetric (TG) analysis and thermogravimetry-infrared-mass (TG-IR-MS) test showed that CPB1 mainly had a gas phase. In contrast, CPB2 mainly had a condensed phase flame retardant mechanism. The mechanical properties of the cotton fabric remain good after treatment.
研究领域:
材料科学
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纺织工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Wen-Xiong Li,Hai-Jun Zhang,Xiao-Ping Hu,Wen-Xue Yang,Zhou Cheng,Chang-Qiong Xie
The intumescent flame retardant (IFR) with ammonium polyphosphate (APP) as the main component has many defects in practical applications, more than that, APP can be traced to non-renewable phosphate rock resources. For the foregoing reasons, the melamine phytate supramolecular nanosheet flame retardant incorporating manganese ion (PAMA-Mn) was successfully prepared with a facile and environmental friendly hydrothermal procedure based on renewable bio-based material phytic acid (PA). The flame retardant polypropylene composite (PPI) with 13.5 wt% APP and 4.5 wt% pentaerythritol (PER) failed to the UL-94 test, and its limiting oxygen index (LOI) value was only 26.5%. After 33 wt% of APP was replaced by PAMA-Mn, the PPMn33 incorporating only 18 wt% flame retardant additives passed the UL-94 V-0 rating, and its LOI value was increased to 31.9%. Compared with PP, pHRR and pSPR values of PPMn33 were reduced by 56% and 23%, respectively. The fire retardant mechanism of PPMn33 was thoroughly discussed via a variety of characterization methods. It was found that the peak of the Gram-Schmidt curve of PPMn33 was drastically reduced by 49% relative to that of PPI, indicating a remarkably decrease of combustible volatile products owing to the incorporation of PAMA-Mn, thereby rapidly reducing the fire hazard risk.
研究领域:
材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
·
2023
·
IF 7.4
Jinxi Zhang,Duowang Yang,Guoqiang Sun,Yiwen Li,Yue Qi,Xiaoyan Ma
Using recycled oils and chemical stabilizers as the additives in high-viscosity modified asphalt (HVMA) is being developed as a sustainable way to solve the severe segregation problems due to the large differences in nature between high-content styrene–butadienestyrene (SBS) and base asphalt. This paper attempts to use three recycled oils, namely castor oil (CO), waste cooking oil (WCO) and recycled engine oil (REO), as compatibilizers in combination with different chemical stabilizers (polyphosphoric acid, PPA; sulfur) to investigate their ameliorative roles on HVMA segregation problems. By means of the gel permeation chromatography, dynamic shear rheometer and fluorescence microscopy, the chemo-rheological properties and morphological structures of HVMA are investigated, as well as their storage stability. Results show that the recycled oils are more favorable to improve the high-temperature rheological performance of HVMA than aromatic oil (AO) by comprehensively considering the softening point, zero-shear viscosity, elastic recovery percent and large molecule percentage, and the role ranking is: REO > WCO > CO > AO. Compared with the addition of PPA or sulfur alone, when PPA and sulfur are added together, the rheological performance of HVMA is promoted most significantly, because of the synergistic cross-linking reaction of these chemical stabilizers with asphalt and polymers. Furthermore, the synergistic effect of PPA and sulfur markedly reduces the temperature sensitivity of HVMA. However, the cigar tube test reflects that adding recycled oils as compatibilizers alone can not improve the storage stability of HVMA, and CO and WCO even aggravate its segregation. Notably, adding REO and chemical stabilizers can markedly improve the storage stability of HVMA, and the effect is most obvious when REO, PPA and sulfur are added together.
研究领域:
土木工程
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER INTERNATIONAL
·
2020
·Vol. 69
·期 10
·985-994
·
IF 2.574
Meng Ma,Xinpeng Wang,Kai Liu,Si Chen,Yanqin Shi,Huiwen He,Xu Wang
The preparation of poly( l -lactic acid) (PLLA) with high mechanical and ideal flame-retardant properties is a great challenge. Herein, a simultaneous toughness and flame-retardant PLLA composite was successfully fabricated by using a one-step process which introduces 4,4′-methylenediphenyl diisocyanate and ammonium polyphosphate (APP) into PLLA/poly(ε-caprolactone) blends. SEM, Fourier transform infrared spectroscopy and TGA were adopted to confirm that APP participated in the in situ reaction during the melt process. The impact strength was increased to 13.5 kJ m −2 from 1.0 kJ m −2 for L8P2A5 composite, indicating the toughening effect of reactive blending. The cone calorimeter test, limiting oxygen index and vertical burning test results indicate that the flame-retardant properties of the composites are enhanced with increasing APP content. This work provides a method to prepare PLLA with high mechanical properties and enhanced flame retardancy. © 2020 Society of Chemical Industry
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
Polymers
·
2022
·Vol. 15
·期 1
·55
·
IF 4.967
Peipei Sun,Ziwen Zhou,Licong Jiang,Shuai Zhao,Lin Li
Enteromorpha, as a waste from marine pollution, brings great pressure to environmental governance every year, especially for China. Under the premise of a shortage of industrial materials, taking appropriate measures can turn waste into wealth, which will benefit us a lot. In this work, a bio-based reinforcing-type flame retardant based on Enteromorpha is designed. The designed Enteromorpha-based flame retardant system (AEG) mainly focuses on the reinforcing and flame retardant effects on ethylene-propylene-diene tripolymer (EPDM). For the AEG system, ammonium polyphosphate (APP) serves as both the acid source and the gas source; the simple hybrid material (GN) produced by loading graphene (GE) and Enteromorpha (EN) using tannic acid (TA) as a regulator serves as an acid source and a carbonizing source. The results show that when 40 phr AEG is added, the LOI of EPDM/AEG40 reaches 32.5% and the UL-94 reaches the V-0 level. The PHRR and THR values of EPDM/AEG40 are 325.9 kW/m2and 117.6 MJ/m2, respectively, with decrements of 67.3% and 29.7%, respectively, compared with the results of neat EPDM composite. Especially, the TSP and TSR values of EPDM/AEG40 are reduced from 15.2 m2of neat EPDM to 9.9 m2with a decrement of 34.9% and reduced from 1715.2 m2/m2of neat EPDM to 1124.5 m2/m2with a decrement of 34.4%, indicating that AEG is effective in flame retardancy and smoke suppression. Meanwhile, the tensile strength and tear strength of EPDM/AEG composites are much higher than neat EPDM, therefore, with the future development of innovate reinforcing-type flame-retardant Enteromorpha, the application of Enteromorpha in the polymer flame-retardant field will surely usher in bright development.Keywords:Enteromorpha;reinforcing;flame retardant
研究领域:
材料科学
·
环境科学
·
聚合物工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
·
2025
·
IF 8
Jinpeng Wang,Yang Su,Xue Yang,Fengrong Xin,Jian Wang,Chaoyi Fan,Zhen Yang,Dandan Peng,Zhehong Shen,Qun Fang,Sai Wu
As a bio-based wood product, plywood is widely used in furniture, flooring, packaging, architectural decoration, etc. However, the flammability of plywood limits its application. Using flame-retardant adhesives to prepare plywood is the simplest way to improve its flame-retardant properties. However, a challenge in flame-retardant adhesives arose from the negative effect of flame retardants on the curing and bonding properties of adhesives. Herein, we developed a method to prepare a flame-retardant adhesive modified by using Z-3A molecular sieves as carriers to load flame retardants such as phytic acid and ammonium polyphosphate. The flame-retardant performance of obtained plywood bonded by this flame-retardant adhesive was significantly improved with robust bonding strength under extraordinarily low loading of flame retardants. Even if the loading rate of ZPA flame retardant for 2-ZPA-5 plywood was as low as 2.2 %, the total smoke production and the maximal peak value of the heat release rate were reduced by 79 % and 14 %, respectively, with the limiting oxygen index being 33.6 %, the time to ignition delaying by 14 s, robust wet bonding strength being 1.79 MPa, anti-loss rate as 1.06 ± 0.18 %, and moisture absorption rate as 4.45 ± 0.14 %. The whole process needs neither tedious work or equipment nor high loading of flame retardants. This judicious design strategy offers a versatile and scalable approach to preparing flame retardant plywood.
研究领域:
材料科学
·
林业工程
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产品关联
DOI
高影响因子
SOIL USE AND MANAGEMENT
·
2019
·Vol. 35
·期 4
·664-674
·
IF 1.958
Xuewei Wang,Yanju Gao,Baowei Hu,Guixin Chu
Polyphosphate is an alternative phosphorus (P) source which can substitute for orthophosphate-based P fertilizers in agriculture. In order to explore the effects of polyphosphate addition on soil P availability, and how pH and temperature affect polyphosphate hydrolysis, an aqueous and a soil incubation experiment were conducted at different pHs (5.0 and 8.2) and temperatures (15, 25 and 35°C); the influence of polyphosphate addition on soil available micronutrients (i.e., iron—Fe, manganese—Mn and zinc—Zn) was also studied. The experiments used three different polyphosphate fertilizers (solid and liquid ammonium polyphosphate and polyphosphoric acid) and two soil types (acid and alkaline). In aqueous incubation conditions, the average amount of phosphate ( ) released from polyphosphate fertilizers at 35°C was 2.7 times greater than at 25°C. The average Olsen P in polyphosphate-treated soil at 35°C was 1.12 times greater than at 25°C, indicating that higher temperature facilitates polyphosphate hydrolysis. Polyphosphate hydrolysis increased as the pH decreased in aqueous solution, but its hydrolysis rate was greater in calcareous soil than in acid soil. Moreover, polyphosphate significantly increased soil available Fe, Mn and Zn concentrations by an average of 14%, 16% and 20%, respectively, relative to orthophosphate fertilizer. In summary, high temperature and low pH favour the hydrolysis of short-chain polyphosphate (aqueous incubation experiment), and polyphosphate significantly increases soil P availability and mobilizes soil micronutrients. The application of polyphosphate can be recommended as a pragmatic P management strategy in agriculture: however, soil temperature and pH should be taken into account when using polyphosphate fertilizers.
研究领域:
农业科学
·
土壤学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）