JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Yu Lu,Wanjun Jin,Yuerong Yang,Yue Jia,Lujia Sun,Jie Liu,Langhong Wang,Fuxing Zhang,Wupeng Ge,Jiansheng Wang,Linjuan Huang,Zhongfu Wang
Goat milk oligosaccharides are complex carbohydrates with a variety of biological functions. Free oligosaccharides from goat milk show more similarity to human milk than cow milk. At present, changes in goat milk glycoconjugates at different parities remain poorly studied. Herein, we qualitatively and quantitatively compared the goat milk glycoprotein N/O-glycome at different parities using a stable isotope labeling followed by electrospray ionization mass spectrometry and online hydrophilic interaction chromatography. N-Glycans were mainly fucosylated and nonfucosylated nonsialylated, and both fucosylation and sialylation gradually increased with parity, amounting (at the third parity) to 1.25 times and 3.3 times those of the first parity, respectively. O-Glycans were mostly nonfucosylated and nonsialylated, and sialylation increased with increasing parity, and Neu5Ac-sialylated was up to 9 times higher in the third parity than in the first parity, whereas Neu5Gc-sialylated was 5.5 times higher. This study provides a reference for exploring an alternative milk source closest to human milk and for the development of humanized formula milk.
研究领域:
食品科学
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生物化学
·
营养学
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DOI
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 7
·1072
·
IF 5.719
Lei Li
Natural biomaterials applicable for biomemristors have drawn prominent attention and are of benefit to sustainability, biodegradability, biocompatibility, and metabolism. In this work, multi-bit biomemristors based on the neutral polysaccharide dextran were built using the spin-casting method, which was also employed to explore the effect of dextran on the ternary biomemristic behaviors of dextran–chitosan nanocomposites. The doping of 50 wt% dextran onto the bio-nanocomposite optimized the ratio of biomemristance in high-, intermediate-, and low-resistance states (105:104:1). The interaction between dextran and chitosan (hydrogen-bond network) was verified by Fourier transform infrared (FTIR) and Raman spectroscopy analysis; through this interaction, protons derived from the self-dissociation of water may migrate under the electric field, and so proton conduction may be the reason for the ternary biomemristic behaviors. Observations from X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis displayed that the 50 wt% dextran/50 wt% chitosan nanocomposite had the greatest amorphous ratio as well as the highest decomposition and peak transition temperatures in comparison with the other three dextran–chitosan nanocomposites. This work lays the foundation for neutral biomaterials applied to green ultra-high-density data-storage systems.
研究领域:
材料科学
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生物工程
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DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 24
·9039
·
IF 4.927
Yijie Zhang,Zhiwen Jiang,Yanting Wang,Lixin Xia,Shuqin Yu,Hongjian Li,Wei Zhang,Wanshun Liu,Kai Shao,Baoqin Han
Chitosan (CTS) has been used as a nerve guidance conduit (NGC) material for bridging peripheral nerve defects due to its biocompatible, biodegradable, and non-toxic properties. However, the nerve regeneration effect of chitosan alone is restricted due to its inadequate biological activity. Herein, a composite, bioactive chitosan based nerve conduit, consisting of outer warp-knitted tube scaffold made from medical-grade chitosan fiber, and inner porous cross linked carboxymethyl chitosan (C-CM-CTS) sponge with radial texture was developed. The inner wall of the scaffold was coated with C-CM-CTS solution. CM-CTS provided favorable bioactivities in the composite chitosan-based nerve conduit. An in vitro study of CM-CTS revealed its satisfying biocompatibility with fibroblast and its inhibition of oxidative damage to Schwann cells. As the internal filler of the NGC, the lyophilized sponge of C-CM-CTS showed a longitudinal guidance effect for nerve reconstruction. After 10 mm defect in rat sciatic nerve was bridged with the composite bioactive chitosan-based nerve conduit, the nerve conduit was able to effectively promote axonal regeneration and played a positive role in inducing nerve regeneration and functional recovery. In addition to the functional advantages, which are equal to those of an autograft; the technology for the preparation of this conduit can be put into mass production.Keywords:chitosan;carboxymethyl chitosan;nerve conduit;biocompatibility
研究领域:
生物医学工程
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神经科学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 16
·8717-8729
·
IF 6.317
Xiao-yan Chang,Yuan-yuan Liu,Meng-meng Hu,You-qian Liu,Cui-hua Jiang,Qi Wang,Qiao-mei Jin,Dong-jian Zhang,Zhi-qi Yin,Jian Zhang
This study aimed to compare the effects of different hydrolysates (named GKOS and MKOS) on constipated rats, which were obtained by degradation from konjac glucomannan by β-glucanase and β-mannanase, respectively. GKOS and MKOS were characterized and administered by gavage at 100 mg kg−1 to constipated rats. The variation of the gut flora, content of short-chain fatty acids (SCFAs), defecation function, gastrointestinal motility, and intestinal mucus secretion were determined to evaluate their regulatory effects on constipation. The results revealed the more prominent augmentation of species richness in MKOS than with GKOS. They also possessed diverse modulatory effects on different genera, such as Prevotella and Parabacteroides. Unexpectedly, there was no statistical divergence between GKOS and MKOS in defecation parameters, gastrointestinal transit, serum parameters, and mucous secretion. Overall, MKOS and GKOS exhibited differential regulatory function on gut microbiota in vivo, but with nearly consistent therapeutic effects on constipation.
研究领域:
药理学
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胃肠病学
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微生物学
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产品关联
DOI
PubMed
高影响因子
POLYMER INTERNATIONAL
·
2020
·Vol. 70
·期 2
·185-192
·
IF 2.574
Yuanyuan Hou,Fei Huang,Hui Yang,Hao Cong,Xi Zhang,Xiaoxu Xie,Haibo Yang,Qiuping Tong,Ning Luo,Ping Zhu,Jianzong Meng
Levansucrase (EC 2.4.1.10) catalyzes both levan polymerization and sucrose hydrolysis. Levan production by recombinant Bacillus subtilis levansucrase SacB-T305A was investigated. The maximum conversion yield (33.09%) of sucrose to levan and levan yield (115.8 g L –1 ) were obtained as 30% (wt/vol) and 40% (wt/vol) initial sucrose, respectively. Under highly acidic conditions (pH 3.5 and below), the polymerization not only shifted distinctly to high molecular weight levan but also enlarged the size of low molecular weight levan. In particular, the proportion of high molecular weight levan to total products increased to 9-fold at pH 3.5. The presence of Fe 2+ , Mg 2+ , Mn 2+ and Zn 2+ ions at common ion strength (5 mmol L –1 ) increased only the size of low molecular weight levan from about 20 kDa to about 35 kDa. Our findings provide a supplementary view about the mechanism of levan molecular weight distribution in enzymatic synthesis and offer a possibility for producing levan of different molecular weights by modulating enzymatic reaction conditions. © 2020 Society of Industrial Chemistry
研究领域:
生物化学
·
工业微生物学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Jun Rao,Ziwen Lv,Gegu Chen,Xiang Hao,Ying Guan,Feng Peng
To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome. Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO). The results of scanning electron microscope revealed the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property. As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively. The oxygen barrier properties of the composite films significantly increased with the addition of graphene oxide due to the dense, stacked multilayer structure. In addition, these composite films exhibited good antibacterial properties . Therefore, these films show great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties .
研究领域:
材料科学
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食品科学
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生物医学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Jingfeng Li,Lifang Chen,Zhiyun Meng,Guifang Dou
Chitosan has multiple biological activities, but a sensitive and rapid characterization method is yet to be developed for its further application. This study presented an optional mass spectrometry method for the characterization of chitosan. Nine kinds of chitosan (degree of deacetylation (DD), 63.08%–89.06% & MW, 106.1–485.0 kDa) were adopted for the method development. Most species of chitosan, detected by an ESI-MS technique, were observed below 1000 m/z , which seemed that only chito-oligosaccharide (COS) impurities were detected. Then, a sensitive UPLC-ESI-MS/MS method was established to assess the COS impurities in chitosan, and no COS impurities were detected. However, dissociation of chitosan and COSs in the ESI source were observed, and then the mass spectra patterns were deeply evaluated via an accurate Q-TOF mass spectrometer. Our research demonstrated that the mass spectra of COSs and chitosan resulted from the dissociation of glycosidic linkage and dehydration. Although the distribution of GlcN and GlcNAc units in these chitosan samples might be different, similar dissociation efficiencies were observed. Furthermore, good linearities were obtained between the intensities of product ions, detected by an UPLC-pseuedo-MS2 method, and DDs determined by conventional method. This method could be suitable for the DD determination and quantitative analysis of chitosan.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Yuanyuan Zhao,Yubin Wang,Qiancheng Ma,Dan Wang,Qianqian Jiang,Pan Wang,Zhiwen Ge,Junjuan Wang,Peiyou Qin,Xiaoyan Zhao
Dietary oligo- and polysaccharides modulate gut microbiota and thus exert prebiotic activity, which is determined by their heterogeneous structure. To explore the correlations between monosaccharide profile and microbial community, simulated gut fermentation of different glycans, including arabinan (ArB), galactooligosaccharide (GOS), arabinogalactan (ArG), rhamnogalacturonan (RhG), and xyloglucan (XyG) that are characterized by typical sugar residues were performed. Results showed that RhG displayed high contents of galacturonic acid (344.79 mg/g), rhamnose (171.70 mg/g), and galactose (151.77 mg/g), and the degradation ratio of them after fermentation was 73.87 %, 84.96 %, and 87.11 %, respectively. Meanwhile, the relative abundance of glycan-degrading bacteria Bacteroides in the RhG was boosted from 4 h (4.97 %) to 48 h (36.45 %). Butyrate-generating bacteria Megasphaera (56.69 %) and Bifidobacterium (28.02 %) are dominant genera in the ArB, which generated the highest concentration of carbohydrate-metabolite (94.58 mmol/L) in terms of acetate, propionate, butyrate and valerate, followed by the ArG (87.36 mmol/L). However, ammonia generation of the ArG increased rapidly, representing the highest content of protein-metabolite (66.36 mmol/L) including ammonia, isobutyrate, and isovalerate. As compared, metabolites generated from protein and carbohydrates grow steadily at a low level during the XyG fermentation. Correlation analysis further indicated that Bacteroide s was positively correlated with propionate ( p < 0.001), galacturonic acid ( p < 0.001), and rhamnose ( p < 0.05), while Bifidobacterium has positive correlation with butyrate and arabinose ( p < 0.01). Overall, monosaccharides composition in the different oligo- and polysaccharides induces distinct responses of the dominant microbiota and thus modulates the subsequent fermentation metabolites of carbohydrate and protein, promoting a deep understanding of the structure-fermentation relationship of dietary glycans.
研究领域:
微生物学
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营养学
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食品科学
·
肠道微生物
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·Vol. 142
·期 14
·e56693
·
IF 2.8
Zi-Ang Xia,Long Wang,Chaojie Li,Xue Li,Jingxue Yang,Baoming Xu,Heng Zhang
The augmentation of chitosan's (CS) antioxidative and antibacterial properties via grafting with phenolic acids constitutes a significant method for expanding the utility of chitosan. In this investigation, four phenolic acids (gallic acid, GA; protocatechuic acid, PCA; hydroxybenzoic acid, HA; and salicylic acid, SA) were conjugated with CS utilizing carbodiimide (EDC)-mediated grafting. The resultant phenolic acid-CS graft copolymers underwent characterization employing infrared spectroscopy, x-ray diffraction, and thermogravimetric analysis. Furthermore, ex vivo antioxidative and antibacterial assays were performed. Findings revealed that among the synthesized phenolic acid-chitosan graft copolymers, gallic acid-chitosan (GA-CS) demonstrated the highest antioxidative and antibacterial efficacy, primarily attributed to its elevated grafting ratio and abundance of hydroxyl groups. The antioxidative and antibacterial performances of the remaining three graft copolymers followed this order: CS-PCA > CS-HA > CS-SA, aligning with the trend in grafting ratios. This correlation arises from the higher grafting ratios of copolymers yielding more active OH groups, thereby exhibiting enhanced antioxidative and antibacterial properties. Phenolic acid-chitosan graft polymers exhibit promising antioxidative and antibacterial attributes, making them suitable additives for application in the food preservation packaging industry.
研究领域:
食品科学
·
材料科学
·
微生物学
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产品关联
DOI
高影响因子
Soft Matter
·
2013
·Vol. 9
·期 46
·11136-11142
·
IF 3.909
Jingjun Wu,Qian Zhao,Chengzhen Liang,Tao Xie
Enzyme-degradable hydrogels with aligned pores were prepared via unidirectional freezing of a mixed solution of oxidized dextran (oxDex) and chitosan (CHI) followed by cryo-gelation. At the frozen state, imine bonds as the crosslinking points of the hydrogels were formed as a result of the reaction between the aldehyde groups on the oxDex and the amino groups on the CHI. The morphologies of the obtained hydrogels were studied by confocal laser scanning microscopy (CLSM). Microtubular pores aligned along the freezing direction were obtained in the swollen state of the hydrogels. The pore size was adjustable ranging from 20 to 100 μm by controlling the freezing temperature in the unidirectional freezing step. Upon exposure of the hydrogels to chitosanase solution, chitosan can be hydrolyzed into short oligosaccharide fragments leading to collapse and degradation of the gel network. The degradation rate can be tuned by adjusting the gel composition and enzyme concentration.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 17
·5691
·
IF 4.927
Lan Luo,Xuemei Song,Xiao Chang,Sheng Huang,Yunxi Zhou,Shengmei Yang,Yan Zhu,Lanyan Zhang,Yongsheng Wu,Junyin Zhang,Zhipeng Zhou,Mingyi Wu
Bacillus Calmette-Guérin polysaccharide and nucleic acid (BCG-PSN), extracted fromMycobacterium bovis, is an immunoregulatory medicine commonly used in clinic. However, the structural characteristics and potential pharmacological efficacy of the polysaccharides from BCG-PSN remain unclear. Herein, two polysaccharides (BCG-1 and BCG-2) were purified and their structures were characterized. Monosaccharide composition analysis combined with methylation analysis and NMR data indicated that BCG-1 and BCG-2 were an α-D-(1→4)-mannan with (1→2)-linked branches, and an α-D-(1→4)-glucan with (1→6)-linked branches, respectively. Herein, the mannan from BCG-PSN was first reported. Bioactivity assays showed that BCG-1 and BCG-2 dose-dependently and potently increased the production of inflammatory mediators (NO, TNF-α, IL-6, IL-1β, and IL-10), as well as their mRNA expressions in RAW264.7 cells; both have similar or stronger effects compared with BCG-PSN injection. These data suggest that BCG-1 and BCG-2 are very likely the active ingredients of BCG-PSN.Keywords:active ingredients;polysaccharide purification;glucan;mannan;immunomodulator
研究领域:
免疫学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Qingxia Yuan,Xiandong Zhang,Mingyang Ma,Tao Long,Chulei Xiao,Jing Zhang,Jikai Liu,Longyan Zhao
In this study, a glucuronoxylomannan named TAP-3 was obtained from high-value Tremella aurantialba Bandoni et Zang. Physicochemical analysis revealed that TAP-3, which had a molecular weight of ∼624 kDa, mainly consisted of d -mannose (Man), d -xylose (Xyl), and d -glucuronic acid (GlcA) in a molar ratio of 3.0:1.0: 1.0. Structural analyses of its depolymerized fragments clarified that TAP-3 contained a (1 → 3) and (1 → 2)-linked α-Man p backbone, side chains formed by β-Xyl p and β-Glc p A linked to the C -2 position of α-Man p , and acetyl groups connected to the sixth hydroxyl positions of Man p . TAP-3 showed marked immune enhancement activity, promoting NO, IL-1β and TNF-α secretion from macrophages. The critical membrane receptor of TAP-3 was identified to be TLR4 , and the chain length was essential for its immunoregulatory activity. These findings expand knowledge of the structural types of glucuronoxylomannan and illustrate its biological activity as an immunopotentiator.
研究领域:
免疫学
·
生物化学
·
真菌学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）