Food Analytical Methods
·
2018
·Vol. 11
·期 11
·3095-3102
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IF 2.245
Wang Hai-Bo,Li Yang,Bai Hong-Yu,Zhang Zi-Ping,Li Yong-Hong,Liu Yan-Ming
In this work, a rapid and label-free fluorescent sensing method has been established for tetracycline (TC) detection by using poly(adenine) (poly A) DNA-templated Au nanoclusters (AuNCs) as fluorescence indicator. The obtained AuNCs exhibited excellent fluorescence intensity and good stability. In the presence of tetracycline, the fluorescence intensity of AuNCs was obviously reduced due to the interactions between the tetracycline and the poly A DNA-templated AuNCs. Under the optimum conditions, the strategy permitted sensitive detection of tetracycline in a linear range from 0.1 to 60 μM and with a limit of detection of 20 nM. In addition, the sensor was successfully applied to determinate the tetracycline content in milk samples. More importantly, it could be easily performed within 10 min at room temperature. Thus, it could provide a rapid, convenient, label-free, and high-sensitive fluorescent platform for tetracycline detection.
研究领域:
分析化学
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纳米技术
·
食品安全
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Guanyu Chen,Wenqian Chen,Lilan Xu,Huixuan Jin,Weiming Sun,Jianming Lan,Fang Wu,Xi Zhang,Jing Zhang,Jinghua Chen
It remains a huge challenge to integrate the sensitivity, stability, reproducibility, and anti-fouling ability of electrochemical biosensors for practical applications. Herein, we propose a self-assembled electrode combining hexanethiol (HT), poly-adenine (poly-A), and cholesteryl-modified DNA to meet this challenge. HT can tightly pack at the electrode interface to form a hydrophobic self-assembled monolayer (SAM), effectively improving the stability and signal-to-noise ratio (SNR) of electrochemical detection. Cholesteryl-modified DNA was immobilized at the electrode through the hydrophobic interaction with HT to avoid the competition between the SAM and the DNA probe on the gold site. Thus, the assembly efficiency and uniformity of the DNA probe as well as the detection reproducibility were increased remarkedly. Poly-A was added on the HT assembled electrode to occupy the unreacted sites of gold to further enhance the anti-fouling ability. The combination of HT and poly-A allows the electrode to ensure favorable anti-fouling ability without sacrificing the detection performance. On this basis, we proposed a dual-signal amplification electrochemical biosensor for the detection of exosomal microRNAs, which showed excellent sensitivity with a detection limit down to 1.46 aM. Importantly, this method has been successfully applied to detect exosomal microRNA-21 in cells and human serum samples, proving its potential utility in cancer diagnosis.
研究领域:
生物医学工程
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分析化学
·
分子诊断
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DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Yan Tan,Zhipeng Wang,Haowen Yin,Peng Yu
The cervical cancer is the fourth most common cancer among women worldwide. Human papillomavirus type 16 (HPV16) is one of the common biomarkers which cause the cervical cancer. In this work, an electrochemical sensor based on a triblock polyadenine-based probe (TPP) and copper nanoclusters (CuNCs) was constructed for the rapid, sensitive and selective detection of HPV16. The TPP contained the central polyadenine (PolyA) segment and two flanking DNA probes. The middle PolyA segment had a high affinity to the gold electrode surface, leading to the adjustable density of the TPP by the length of PolyA. When the DNA template that mediated the formation of CuNCs was introduced, the DNA template partly hybridized with the two flanking DNA probes of the TPP. A large number of CuNCs were synthesized on the DNA template, producing a big electrochemical impedance signal. When the target DNA (HPV16) was present, it bound to the two flanking DNA probes of the TPP, which resulted in the release of the DNA template from the electrode and produced a small electrochemical impedance signal. The limit of detection (LOD) for the detection of HPV16 was 3.34 pM and the linear range was 10 pM - 10 μM. The designed sensor demonstrated good sensitivity, good selectivity, and satisfied recovery, providing the valuable insights in the cervical cancer prevention and the development of the electrochemical biosensors.
研究领域:
生物传感器
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纳米技术
·
分子诊断学
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电化学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 33
·18728-18733
·
IF 3.049
Jinjin Yin,Jiuchao Wang,Xiyue Yang,Tao Wu,Huashan Wang,Xiaoming Zhou
In this work, a facile and sensitive colorimetric sensor for Hg2+ ions based on poly (adenine)-mediated DNA-functionalized gold nanoparticles (Au NPs) is reported. One DNA sequence consisting of poly-A and T-rich DNA was designed rationally. Poly-A was used as an anchoring block to bind tightly to Au NPs, and T-rich DNA was utilized for specific recognition of Hg2+ ions. With the assistance of poly-A, T-rich DNA was easily introduced onto the surface of Au NPs and kept an upright orientation. In the presence of Hg2+ ions, T base binding with Hg2+ ions results in the formation of “T–Hg2+–T” among the Au NPs, which caused aggregation of the Au NPs and a subsequent change in the color of the solution, from wine red to grayish blue. On this occasion, the limit of detection (LOD) was 3.75 nM Hg2+ ions with a linear range from 5 nM to 200 nM, as measured by UV-Vis spectroscopy. Moreover, successful application of this method for the detection of Hg2+ ions in real samples was demonstrated.
研究领域:
分析化学
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环境科学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Yu Huang,Rui Liu,Chin-Jung Chuang,Linhao Jiang,Qiongyuan Zhang,Jiangling Wu,Zhiguo Wu,Weiguang Yang,Qianye Zhang,Yuyan Sun,Ling Pan,Dongmei Liu,Yue Li,Xing Chen,Lei Feng,Hua Zhang,Kaiji Xie
Acting as a pivotal cytokine of innate immunity, the signal transduction of interleukin 6 (IL-6) is always elevated in relation to the occurrence of injury, bacteria and chemical invasion. IL-6 as a critical care biomarker can be used for the clinical diagnosis of diseases ranging from bacterial infections to most inflammatory reactions. Then, a simple, cost-effective and sensitive colorimetric aptasensor was designed for the specific detection of IL-6. The IL-6 aptamer anchored on gold nanoparticles (AuNPs) by polyadenine accurately recognized IL-6, and then, the folded IL-6 aptamer enhanced the stability of AuNPs against salt-induced aggregation. The degree of AuNP aggregation increased inversely with the concentration of IL-6. A higher IL-6 content resulted in lower aggregation of AuNPs, which can be measured by spectrophotometry. The results showed that the proposed aptasensor exhibited a linear detectable range of 7–500 pg mL−1 with a limit of detection of 4.652 pg m−1. Furthermore, this proposed colorimetric aptasensor achieved the discrimination of IL-6 from other analogues and could detect IL-6 in human serum samples, which demonstrated its potential application for the detection of IL-6 in complex matrices.
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Zhehan Yang,Zhenjia Yang,Xiaoshu Lv,Guangming Jiang,Xiaolong Zhang,Xiaodan Li,Xiaoyu Li,Ruo Yuan,Ying Zhuo
Quantifying trace markers in clinical samples with electrochemical impedance spectroscopy (EIS) biosensors is limited mainly by ubiquitous biofouling because traditional antifouling coatings suffer from low stability and unsatisfactory antifouling capability etc. Herein, a simple and effective strategy was constructed to achieve a highly specific EIS biosensing platform for quantifying protein and DNA in serum by using single-stranded oligonucleotides (ssDNA) as antifouling coating. To achieve the optimized antifouling capability of ssDNA coating, the influence factors of ssDNA structure are investigated by combining electrochemical experiments with theoretical calculations. The results demonstrated that the chemical adsorption of adenine on the Au surface is stronger than that of the other three bases, which endows the poly adenine-ssDNA (polyAn) coating with outstanding antifouling capability. Importantly, we found that the capture ligand structure and ssDNA length remarkably affected the antifouling capability of polyAn because of steric hindrance. As a proof of concept, EIS biosensors based on polyAn antifouling coating were developed to detect target DNA and nuclear factor kappa b p65 (NF-kB p65), respectively, exhibiting outstanding capability of detecting DNA and NF-kB p65 in human serum with low interference and good accuracy. Overall, the presented work may contribute to a deeper understanding of the influence factors of ssDNA-based antifouling coating and holds great insight into the construction of highly specific biosensors for quantifying disease markers in clinical samples.
研究领域:
生物传感器
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分析化学
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生物化学
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分子生物学
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临床诊断
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）