Xi Ba,Liqiang Luo,Yaping Ding,Zhen Zhang,Yuliang Chu,Bijun Wang,Xiaoqian Ouyang
In this work, a poly(alizarin red)/Graphene composite film modified glassy carbon electrode (PAR/Graphene/GCE) was prepared for simultaneous determination of four DNA bases (guanine, adenine, thymine and cytosine) without any pretreatment. The morphology and interface property of PAR/Graphene films were examined by scanning electron microscopy and electrochemical impedance spectroscopy. The PAR/Graphene/GCE exhibited excellent electrocatalytic activity toward purine (guanine and adenine) and pyrimidine (thymine and cytosine) in 0.1 M phosphate buffer solution (pH 7.4). Under optimum conditions, differential pulse voltammetry was used to detect the oxidation of purine and pyrimidine. The results showed that PAR/Graphene/GCE exhibited well-separated peaks, low detection limit, high sensitivity and wide linear range for simultaneous detection of purine and pyrimidine. The proposed sensor also has good stability and reproducibility. Furthermore, the modified electrode was applied for the detection of DNA bases in a fish sperm DNA sample with satisfactory results.
A glassy carbon electrode modified with poly(alizarin red)/electrodeposited graphene (PAR/EGR) composite film was prepared and applied to detect ciprofloxacin (CPFX) in the presence of ascorbic, uric acid and dopamine. The morphology and interface property of PAR/EGR films were examined by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The electrocatalytic oxidation of CPFX on AR/EGR was investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The linearity ranged from 4 × 10 −8 to 1.2 × 10 −4 M with a detection limit (S/N = 3) of 0.01 μM. The modified electrode could be applied to the individual determination of CPFX as well as the simultaneous determination of CPFX, ascorbic acid, uric acid and dopamine. This method proved to be a simple, selective and rapid way to determine CPFX in pharmaceutical preparation and biological media.
In this study, nitrofurazone (NF), a commonly used but banned drug in aquaculture, was used as the research model for nitrofuran antibiotics. A poly-alizarin red modified glassy carbon electrode (PAR/GCE) was prepared by electropolymerization. On this modified electrode, NF can produce a sensitive and stable reduction peak. Other nitrofuran antibiotics, such as nitrofurantoin and furazolidone, showed similar responses. The reduction peak of NF was proportional to the concentration within the range of 3.0–50.0 and 50.0–200.0 μM, and the detection limit was 0.33 μM. The linear ranges of furantoin and furazolidone were 10–40 and 50–140 μM, respectively, with corresponding detection limits of 0.73 and 1.56 μM. The modified electrode was used to detect NF in the cultured water sample, and the recovery rate of the sample was 94.5%–104.8%. Thus, the result was satisfactory.
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