COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Jérémie Courtois,Chunyan Li,Chen Wang,Hongping Zhang,Heping Jin,Wei Feng
During the past decade, a large variety of materials dedicated to uranium seawater extraction has been developed for harvesting purposes. Yet, materials especially designed for decontamination following nuclear accidents are scarce and eco-friendly ones even rarer. In this study, hybrid microparticles made of biocompatible calcium carbonate and ε-poly(lysine) are used as efficient U sorbent in seawater. Their eco-friendly nature makes them interesting for both harvesting and on-site immobilization of radionuclides, also accounting their ability to settle in the seabed with limited impact on eco-systems. The material shows excellent U extraction capability in contaminated seawater ([U] = 10 ppm) with removal rate R of 87.7 % and extraction capacity of 513 mg per g of ε-poly(lysine) and remains efficient in natural condition ([U] = 2.8 ppb) with R = 39.8 %. In addition, the sorbent has a remarkable selectivity toward U versus V in U contaminated seawater as well as a significant affinity for Sr 2+ . DFT and XPS analyses revealed that U is adsorbed to ε-poly(lysine) via a chelation mechanism that involves amide and amine groups of lysine. Lability of the formed coordination complex allows easy recovery of captured U by contact with NaHCO 3 desorbing solution, ensuring recyclability of the material.
研究领域:
环境科学
·
材料科学
·
放射化学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER INTERNATIONAL
·
2023
·Vol. 72
·期 8
·720-726
·
IF 3.2
Jie Tian,Nan Wang,Wenxiu Teng,Shuang Song,Jie Pang,Chunhua Wu,Chengrong Wen
The binding of oppositely charged polyelectrolytes is a well-known gel strategy, and pH is an important factor affecting the gel properties. In this work, the influence of pH (3, 5, 7, 9, 11) on the rheological and textural properties, molecular structure and micromorphology of binary mixed gels of ι -carrageenan (IC) and ε -polylysine (PLL) or ε -polylysine hydrochloride (PLH) was investigated. The results showed that a change in pH led to a change in the properties of binary mixed gels. The lower the pH, the higher were G ′, G ″, complex viscosity and gel transition temperature. At the same time, the water holding capacity and gel strength of IC/PLL (IP) and IC/PLH (IPH) were also improved accordingly, and the gel properties of IPH were always better than those of IP. As the pH increased to alkalinity, the density of the gel network structure decreased, the pore size decreased, the pore wall became thinner and the surface smoothed. When the pH dropped into the acidic range, there was a red shift of the hydroxyl group and the sulfate group bands, an enhancement of the hydrogen bonding interaction and a blue shift of the methyl band and the amide I band. At pH 3, the microscopic network structure of IP and IPH were dense and uniform with small pore size, rough surface and thick pore wall. These results showed that the binary mixed gels had better gel properties at pH 3. The results provided a theoretical basis for the preparation of IP and IPH gels, and also enabled a deeper understanding of the mechanism of interaction between polypeptides and carrageenan. © 2023 Society of Industrial Chemistry.
研究领域:
食品科学
·
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2022
·
IF 5.318
Fen Yan,Jinfu Zhong,Junying Chen,Wenjing Liu,Xiaojie Chen
Alginate oligosaccharide (AOS) was enzymatically prepared and had a degree of polymerization (DP) of 2–6. AOS exhibited evident antioxidant efficacy in ABTS + scavenging activity, hydroxyl radical scavenging capacity, and total reducing power. The freshness of the prawns, which were treated with AOS and ε-polylysine (ε-PL), was evaluated by measuring indicators such as total viable bacteria (TVC), pH, total volatile basic nitrogen (TVB-N), sensory, texture, and melanosis during storage period. The texture properties and sensory scores of prawns treated with AOS and ε-PL were significantly improved ( P < 0.05) in comparison with the control group. Meanwhile, the increase in TVC, pH, TVB-N, and melanosis scores of prawns treated with AOS and ε-PL were effectively delayed ( P < 0.05) in comparison with control group. Moreover, the quality of prawns treated with 8 g/L AOS was better than that of prawns treated with 6 g/L. AOS could prolong the shelf life of prawns by 2 days at least. Therefore, AOS is a natural biological preservative in providing an effective and safe way to preserve freshness of prawns.
研究领域:
食品科学
·
海洋生物学
✓ 已验证
DOI
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Dongyi Xian,Rui Luo,Qiaoni Lin,Liqing Wang,Xiaoqian Feng,Yuwei Zheng,Liming Lin,Jiaying Chi,Yilang Yan,Guilan Quan,Tingting Peng,Zejun Xu,Chuanbin Wu,Chao Lu
Antimicrobial peptides show promise in enhancing photothermal therapy, but their application is often limited by the challenge of constructing a delivery system that balances efficacy and safety. Our research demonstrated that the bactericidal efficacy of V 2 C MXene-mediated photothermal therapy is enhanced in a concentration-dependent relationship with the introduction and coating of the antimicrobial peptide ε-polylysine (EPL). EPL exhibited a dual role in enhancing bacterial binding and disrupting bacterial membranes, thereby increasing heat transfer efficiency and reducing bacterial resistance to photothermal ablation. The core strategy of this study was to exploit the combined membranolytic-photothermal effect of EPL and V 2 C by extensively applying EPL while regulating V 2 C nanosheets usage to prevent overheating. This approach aims to achieve potent bactericidal efficacy through photothermal therapy below 60 °C. Consequently, we developed dissolving microneedles incorporated with V 2 C nanosheets, where EPL served as the antimicrobial agent and primary matrix, increasing its loading capacity and minimizing the need for inactive excipients. Notably, this microneedle achieved a 99.9 % reduction in the abundance of methicillin-resistant Staphylococcus aureus on infected skin after a single application and resulted in a 92-fold reduction in the bacterial load compared to the group treated with commercial Bactroban ointment, with no apparent toxicity to the mice.
研究领域:
生物医学工程
·
微生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Hang Jia,Yaping Zhang,Qi Guo,Xuejun Zeng,Yahong Yuan,Zhouli Wang,Zhenpeng Gao,Tianli Yue
In this work, polyacrylic acid modified ferroferric oxide (Fe 3 O 4 -PAA) was synthesized via a one-step hydrothermal method. The magnetic nanospheres modified with carboxyl groups were combined with epsilon-polylysine (EPL) via an EDC/NHS coupling reaction to obtain Fe 3 O 4 -PAA-EPL nanospheres. Fe 3 O 4 -PAA-EPL was employed as an antibacterial agent against Alicyclobacillus acidoterrestris and characterized by XRD, FTIR, XPS, VSM, SEM and TEM techniques. Experimental results showed the minimum inhibition concentration (MIC) of Fe 3 O 4 -PAA-EPL against A. acidoterrestris was 1.25 mg mL −1 . Furthermore, A. acidoterrestris treated with Fe 3 O 4 -PAA-EPL nanospheres obviously lysed. Morphological analysis of bacteria supported by SEM indicated that the cell membrane of A. acidoterrestris was damaged, revealing that Fe 3 O 4 -PAA-EPL is an effective antibacterial agent. Additionally, the nanospheres with excellent magnetism can be simply separated from a reaction system via an external magnet. The construction of magnetic nanospheres with satisfactory antibacterial activity provides an effective and new method to control A. acidoterrestris .
研究领域:
微生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials
·
2022
·Vol. 15
·期 6
·2327
·
IF 3.748
Zuolong Yu,Deping Gong,Chao Han,Yunxiao Wei,Changchun Fu,Xuejiao Xu,Youri Lu
The composite films comprising pea starch (St) and ε-polylysine (PL) as the matrix and glycerol and sodium alginate as the plasticizers were investigated. The rheological properties, mechanical properties, Fourier transformed infrared spectroscopy, water vapor permeability (WVP), oil permeability, microstructure, thermogravimetry (TGA), and antimicrobial properties of the composite films were analyzed. The properties of the composite films with different mass ratios of St/PL varied significantly. First, the five film solutions were different pseudoplastic fluids. Additionally, as the mass ratio of PL increased, the tensile strength of the blends decreased from 9.49 to 0.14 MPa, the fracture elongation increased from 38.41 to 174.03%, the WVP increased, and the oil resistance decreased substantially. The films with a broad range of St/PL ratios were highly soluble; however, the solubility of the film with a St/PL ratio of 2:8 was reduced. Lastly, the inhibition ofE. coli,B.subtilis, and yeast by the films increased with increasing mass ratios of PL, and the inhibition ofB.subtiliswas the strongest.Keywords:pea starch;ε-poly lysine;composite film;antibacterial;properties
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ying Mao,Xinmei Liu,Leiting Xin,Pei Cai,Wangyang Lu,Wenxing Chen
Bacterial infection remains a thorny problem in food, medicine, and other fields. Due to the emergence of antibiotic-resistant bacteria, it is critical to develop an effective strategy to yield novel antibacterial agents. Herein, a new type of non-antibiotic antimicrobial material was successfully synthesized by grafting ε-polylysine (EPL) onto silk fibroin (SF). The resulting ε-polylysine-modified silk fibroin (SF-EPL) possessed the possibility to be processed into different formats. As the EPL content increased, SF-EPL exhibited higher positive charge levels, similar to those of EPL. Notably, the incorporation of 10 % EPL endowed superior antibacterial effects against E. coli and S. aureus (>90 %), while maintaining excellent biocompatibility. Furthermore, SF-10%EPL effectively extended the shelf life of cherry tomatoes, significantly delaying weight loss and the decline in titratable acidity content. The obtained SF-EPL may represent a promising substitute for food preservation and biomedical applications.
研究领域:
生物学
·
医学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Packaging and Shelf Life
·
2023
·
IF 8
Xiaofang Zhao,Zhaolian Han,Shanshan Zhang,Gulijiannaiti Abuduaini,Xin Wen,Tingting Liu,Zhiqiang Cheng
This study aimed to investigate the physicochemical properties, antioxidant and antibacterial activities of Tremella polysaccharides combined with soybean protein isolate (TFP-SPI), natural antibacterial agent ε-polylysine (PL) and polyvinyl alcohol (PVA) hybrid membrane and its applicability in the blueberry preservation. In this study, TFP-SPI was successfully prepared, and it was mixed with PL and PVA to prepare nanofiber membranes. FTIR results showed that TFP-SPI had hydrogen bond interaction. The physicochemical properties showed that the incorporation of TFP-SPI improved the hydrophilicity and mechanical properties of the membrane, providing the best water vapor barrier performance. In vitro antioxidant experiments showed that PVA/TFP-SPI/PL membranes possessed antioxidant capacity . In vitro antibacterial experiments showed that PVA/TFP-SPI/PL membranes had antibacterial activity, especially against Staphylococcus aureus . The results of fruit preservation showed that PVA/TFP-SPI/PL membrane could effectively extend the shelf life of blueberries and maintain good quality. Therefore, PVA/TFP-SPI/PL membrane is a potential active packaging material with promising application prospects.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2013
·Vol. 97
·期 17
·7597-7605
·
IF 3.689
Xia Jun,Xu Hong,Feng Xiaohai,Xu Zhaoxian,Chi Bo
Poly(ε- l -lysine) (ε-PL) producer strain Streptomyces albulus PD-1 secreted a novel polymeric substance into its culture broth along with ε-PL. The polymeric substance was purified to homogeneity and identified. Matrix-assisted laser desorption ionization-time of flight mass spectrometry and nuclear magnetic resonance spectroscopy as well as other analytical techniques revealed that the substance was poly( l -diaminopropionic acid) (PDAP). PDAP is an l -α,β-diaminopropionic acid oligomer linking between amino and carboxylic acid functional groups. The molecular weight of PDAP ranged from 500 to 1500 Da, and no co-polymers composed of l -diaminopropionic acid and l -lysine were present in the culture broth. Compared with ε-PL, PDAP exhibited stronger inhibitory activities against yeasts but weaker activities against bacteria. ε-PL and PDAP co-production was also investigated. Both ε-PL and PDAP were synthesized during the stationary phase of growth, and the final ε-PL and PDAP concentration reached 21.7 and 4.8 g L -1 , respectively, in fed-batch fermentation. Citric acid feeding resulted in a maximum ε-PL concentration of 26.1 g L -1 and a decrease in the final concentration of PDAP to 3.8 g L -1 . No studies on ε-PL and PDAP co-production in Streptomyces albulus have been reported previously, and inhibition of by-products such as PDAP is potentially useful in ε-PL production.
研究领域:
微生物学
·
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2022
·Vol. 40
·期 9
·1110-1119
·
IF 3.815
Teng Lin,Shao Zheng-Wei,He Yu-Shi,Lu Jia-Yu,Zou De-Rong,Feng Chuan-Liang,Dong Chang-Ming
Commercial tissue adhesives have been widely applied in wound hemostats and dressings while enhancing the hemostasis and healing capabilities is challenging to meet clinical needs. Herein, we designed the glucose- and catechol-functionalized derivatives from commercial ε -polylysine (EPL) and prepared the hydrogels by simple amidation and catechol-crosslinking reactions, which have larger swelling ratios of 220%–240%, suitable microporous size of about 6–8 µ m, and tissue adhesion strength of about 20–40 kPa. The hemolysis, cytotoxicity, and cellular double-staining assays indicate that those hydrogels had good biocompatibility and the H-3 hydrogel with higher glucose content gave a lower hemolysis ratio of 0.73%±0.14%. The blood-clotting index, blood cell attachment and adhesion studies showed those hydrogels had fast blood-coagulation, resulting in excellent hemostasis performance with a short hemostatic time of 38–46 s and less blood loss of 19%–34% in a liver hemorrhage model. A full-thickness rat-skin defect model further demonstrates that the H-3 hydrogel achieved fast wound healing with a wound closure of 70.0%±2.7% on postoperative day 7 and nearly full closure on day 14. Remarkably, the hydroproline level that denotes the collagen production reached a higher one of 7.24±0.55 µg/mg comparable to that in normal skins on day 14, evidencing the wound healing was close to completion in the H-3 treatment. Consequently, this work provides a simple method to construct a glycosylated and catechol-functionalized hydrogel platform from commercial EPL, holding translational potentials in wound hemostats and dressings.
研究领域:
生物材料
·
组织工程
·
止血
·
伤口愈合
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Liming Ge,Zijing Li,Miao Han,Yongjie Wang,Xinying Li,Changdao Mu,Defu Li
Food security is an important global public health issue, which will not only endanger consumers’ life and health, but also cause serious food waste. Herein, antibacterial dialdehyde sodium alginate/ ε -polylysine microspheres (DSA-PL MPs) were developed to effectively prolong the shelf life of fruit. DSA was prepared by periodate oxidation of sodium alginate. Then the PL was conjugated onto DSA backbone via the Schiff’s base reaction to synthesize DSA-PL conjugates, followed by the emulsification and Ca 2+ ions crosslinking to obtain DSA-PL MPs. The results indicate that DSA-PL MPs show smooth spherical particle, relatively narrow size distribution and good dispersity. In vitro degradation rate of DSA-PL MPs is higher in acetate buffer (pH = 5.0) than that in PBS buffer (pH = 7.4), showing acid-sensitive degradation property. Significantly, DSA-PL MPs possess strong broad-spectrum antibacterial activity, which can effectively extend the shelf life of fruit. Overall, DSA-PL MPs possess promising application as antibacterial agents for fruit preservation.
研究领域:
食品科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jia-Ning Liu,Sen-Lin Chang,Peng-Wei Xu,Ming-Hui Tan,Bing Zhao,Xiao-Dong Wang,Qing-Sheng Zhao
ε-Poly-l-lysine (ε-PL) consists of 25–35 lysine residues which are linked by an isopeptide bond formed by dehydration condensation of α-carboxyl and ε-amino groups and has good antibacterial activity and broad-spectrum inhibition range. However, there is no clear conclusion about the structure and antibacterial mechanism of ε-PL in aqueous solution. Herein, a high purity of ε-PL was prepared using Amberlite IRC-50 ion-exchange resin. Membrane filtration and dynamic light scattering were used to study the variations of ε-PL aggregation in aqueous solution with pH value. The conformational changes and antibacterial activities of ε-PL and carbamoylated ε-PL in different water environments were studied with circular dichroism (CD) and inhibition zone. The structural changes during the spray-drying process were determined by Fourier transform infrared spectroscopy. The results indicated that the side chain amino charge played a decisive role in the ε-PL conformation and aggregation. ε-PL exhibited the properties of a β-sheet during spray drying from acidic liquids to solids. The cation enhanced the antibacterial activity of ε-PL but did not play a key role. Instead, the backbone of ε-PL might determine the mechanism of ε-PL antibacterial.
研究领域:
生物化学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）