INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Rui Jiang,Song Zhao,Langkun Chen,Maoyu Zhao,Wenjing Qi,Wensheng Fu,Lianzhe Hu,Yan Zhang
In this research, a new DSA (Distyryl-anthracene) derivative with four carboxyl groups was designed and synthesized. This molecule exhibits aggregation-induced emission property (AIE). With the AIE character, a convenient and sensitive fluorescent probe for the detection of protamine , heparin and heparinase has been developed. The protamine can directly induce the aggregation of probe, which caused by electrostatic attraction. In this way, the turn-on detection of protamine is achieved, and the detection limit is as low as 30 ng mL −1 . When heparin appears, the probes will be redisperse in solution, which causes a decrease in fluorescence intensity. Besides, this method also shows good selectivity and sensitivity, and the linear range of heparin is from 0.08 to 8 μg mL −1 with detection limit of 37 ng mL −1 . After hydrolyzing heparin by heparinase, the probes rebind with protamine and the fluorescence enhance. The fluorescence enhancement was linearly related to the concentration of heparinase in the range of 0.02–2.0 μg mL −1 and detection limit as low as 143.7 ng mL −1 . In addition, the results exhibited that the recovery percentage of heparinase in bovine samples reached to 96–101%.
研究领域:
生物化学
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分析化学
✓ 已验证
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
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2022
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IF 9.224
Sihui Wan,Qu Zeng,Shujuan Yang,Xinyu Zhao,Qiyan Wen,Haiyun Tang,Yamei Zhang,Junhao Huang,Gaobo Yu,Yuhong Feng,Jiacheng Li
Pickering emulsions stabilized by polysaccharide–polysaccharide complexes are attractive in many applications. However, how interface films formed by soft particles affect the stability of Pickering emulsions has not been well explored. Herein, we designed aggregation-induced emission (AIE)-active modified alginate (Alg-AIE)/chitosan (CS) polyelectrolyte complex (Alg-AIE/CS complex) colloidal particles that are used to stabilize Pickering emulsions. To explore the relationship between the interface film structure and emulsification properties, the microstructure of emulsions was directly visualized by a combination of confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). The wetting properties of Alg-AIE/CS complexes were regulated by changing the amount of CS, which further regulated the aggregation behavior of the interface. The interfacial adsorption behavior of the particles and the physical stability of the emulsions were determined by combining stability analysis and employing a quartz crystal microbalance with dissipation monitoring (QCM-D). The results showed that the addition of CS resulted in more Alg-AIE/CS complexes that could be adsorbed at the oil–water interface, forming thicker and stronger viscoelastic interface films, which improved the stability of the emulsion. Most importantly, in situ visualization technology provided a new way to understand the interfacial properties of Pickering emulsions.
研究领域:
食品科学
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材料科学
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表面化学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
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IF 5.4
Na Liang,Yingxue Xu,Wei Zhao,Zhenrong Liu,Gang Li,Shaoping Sun
In this study, an amphiphilic polymer FA-CS-DBA-CHO with aggregation-induced emission (AIE) feature was prepared by introducing 4-(diphenylamino)benzaldehyde derivative (DBA-CHO), imine bond and folic acid (FA) to the molecular structure of chitosan (CS). The amphiphilicity drove the polymer to self-assemble into micelles, and paclitaxel (PTX) could be solubilized in the hydrophobic core. Due to the excellent AIE effect, FA-CS-DBA-CHO exhibited strong cellular imaging capability. The pH-sensitive imine bond in the polymer allowed for accurate drug release in acidic environment. Both in vitro and in vivo studies demonstrated that the PTX-loaded FA-CS-DBA-CHO micelles could significantly inhibit the growth of tumor cells but without any notable toxicity. This micellar system was excellent carrier for bioimaging and chemotherapeutic drug delivery.
研究领域:
材料科学
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生物学
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医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2020
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IF 4.389
Jin-Hua Xue,Ke-Ping Xiao,Yong-Sheng Wang,Ling Liu,Jia-Qian Li,Mei Li,Yue-Ning Qu,Xi-Lin Xiao
In this study, we confirmed that protamine-templated gold nanoclusters (PRT-AuNCs) exhibit aggregation-induced emission properties (AIE-PRT-AuNCs). 1-Hydroxypyrene (1−OHPy) further induced the aggregation of AIE-PRT-AuNCs via hydrogen bonding and electrostatic and hydrophobic interactions, resulting in the aggregation-induced photoluminescence enhancement of AIE-PRT-AuNCs. 9-Hydroxyphenanthrene was able to decrease the background signal, thus increasing the sensitivity of the method. Based on these findings, a cost-effective, highly sensitive and selective strategy was proposed for the quantitative detection of 1−OHPy. This method displayed a wide linear range of 0.924 – 74.1 nmol/L with a low detection limit of 0.277 nmol/L, showing great potential for the monitoring of 1−OHPy in human urine. This strategy may provide a theoretical basis for future studies of the AIE properties of metal nanoclusters and their applications in the field of chemical and biological sensing.
研究领域:
分析化学
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生物医学工程
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
TETRAHEDRON
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2021
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IF 2.457
Xuwei Zheng,Jianan Dai,Baoxing Shen,Xing Zhang
An aggregation induced emission (AIE) fluorescent protein chromophore derivative CIC-FP for detecting protamine and heparin under physiological environment was designed and synthesized. CIC-FP exhibited good biocompatibility and a large stoke shift (91 nm), thus well attenuating the background interference, which could be used for quantitative detection of heparin and protamine . Since protamine has strong positively groups, it can interact with CIC-FP where bearing carboxyl through electrostatic interactions to form a tightly bound CIC-FP/protamine complex, thus showing superior fluorescence intensity response with a detection limit of 65.28 ng/mL. Because heparin has a stronger affinity with protamine, it would compete with CIC-FP on protamine, resulting in the release of CIC-FP and making CIC-FP change into free state with decreased fluorescence intensity . Besides, CIC-FP was successfully applied to detect spiked protamine in human serum samples. In summary, CIC-FP can be used to sensitive detection of protamine with good accuracy, which provides a new idea for designing protamine probes.
研究领域:
生物化学
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医学检测
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
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2021
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IF 9.381
Haohui Shi,Na Liang,Jiyang Liu,Siyi Li,Xianfeng Gong,Pengfei Yan,Shaoping Sun
In this study, a novel polymer based on aggregation-induced emission (AIE) fluorogen , biotin and disulfide bonds modified chitosan (TPE-bi(SS-CS-Bio)) was designed and synthesized. The polymer could self-assemble into micelles in aqueous media and encapsulate paclitaxel (PTX) into the core with high drug loading. Fluorescence study indicated that the micelles exhibited excellent AIE feature with intense blue fluorescence emitted. In vitro drug release study indicated that the micelles could disassemble rapidly in the presence of high level of glutathione . The modification by biotin could enhance the cellular uptake of the micelles. The drug-loaded micelles possessed remarkable cytotoxicity against MCF-7 cells, and their distribution in the cells could be traced due to the excellent AIE feature. In vivo antitumor efficacy study demonstrated the superior antitumor activity of the PTX-loaded TPE-bi(SS-CS-Bio) micelles. These results indicated that TPE-bi(SS-CS-Bio) has the ability of biological imaging and can be used as a potential carrier for PTX.
研究领域:
药剂学
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生物医学工程
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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