FOOD CHEMISTRY
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2020
·
IF 6.306
Dong Zhang,Hongjun Li,Zefu Wang,A.M. Emara,Ying Hu,Zhifei He
The effects of oxidation degree on the isoelectric point (pI), aggregation, and structural characteristics for pork myofibrillar protein (MP) were studied by employing extracted MP, which was incubated by using a hydroxyl radical oxidation system. The concentrations of hydrogen peroxide (H 2 O 2 ) were 0, 0.5, 1, 3, 5, 10, and 20 mM. With the increased oxidation degree, the contents of α-helix, ionic bonds, and hydrogen bonds decreased significantly ( P < 0.05). Moreover, the pI value and total amino acids showed a declining trend, and the β-sheet as well as solubility rised firstly and then declined. On the contrary, random curl, β-turn, and turbidity increased significantly ( P < 0.05). Therefore, amino acid side chain groups were modified, and the opposite effect, caused by oxidation that leads to protein cross-linking and aggregation, was greater than the promotion effect, such as net negative charge, these are the main factors that leads to the instability of protein solution systems.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
POLYMER BULLETIN
·
2022
·Vol. 80
·期 1
·495-514
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IF 2.843
Chen Xiuqiong,Zhu Qingmei,Liu Zhaowen,Zhang Tong,Gong Cuiyu,Li Juan,Yan Huiqiong,Lin Qiang
To change the strong hydrophilicity of alginate and make it have good amphiphilic property, thereby broadening its application as a new pesticide-controlled release system, the hexyl alginate ester derivative (HAED) was synthesized by the bimolecular nucleophilic substitution (S N 2) reaction, using 1-bromohexane as the hydrophobic modifier. The structure and self-aggregate performance of the synthesized HAED were contrastively characterized. Moreover, the loading and release performance of λ-Cyhalothrin (LCH) for the HAED microcapsules prepared by the high-speed shearing method was also studied. The synthesized HAED exhibited enhanced molecular flexibility and good self-aggregate performance, which could form the stable self-aggregated spherical micelles with the average hydrodynamic diameter and zeta potential of 416 nm (PDI = 0.29) and − 39.9 mV, respectively, due to the intra- or intermolecular hydrophobic associations. Furthermore, the obtained HAED microcapsules displayed the high encapsulation efficiency and sustained-release property in comparison with the SA microcapsules for the good affinity of HAED to hydrophobic LCH that retarded the drug diffusion. On the basis of the excellent advantages of HAED, it was expected to become the ideal candidate for hydrophobic drug delivery in controlled-release pesticide formulations.
研究领域:
农药化学
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材料科学
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药物传递系统
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产品关联
DOI
高影响因子
Food Bioscience
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2022
·
IF 5.318
Jia-Xin Lin,Tao Xiong,Zhen Peng,Mingyong Xie,Fei Peng
Hyperuricemia has been identified as the second most common metabolic disease, increasing the incidence of gout, hypertension, kidney disease, and cardiovascular disease. Lactic acid bacteria have been attracting considerable interest in anti-hyperuricemia given various probiotic functions. In this study, sixteen lactic acid bacteria were selected from one hundred ninety-two isolates through whole-cell catalysis, affording β-D-glucosidase activity of 0.1652–0.3346 U/mg wet cells, degradation rate of inosine-transformed rate of 1.2125–4.6555 mM/h and guanosine-transformed rate of 0.2904–1.8303 mM/h. The sixteen candidates’ acid tolerance, bile resistance, hydrophobicity , and auto-aggregation were further evaluated. According to the results of the seven indexes, the five top-scored strains were viewed as the potentially functional probiotics for anti-hyperuricemia via the comprehensive evaluation of principal component analysis . Cell assay showed that the adhesion of the five strains to Caco-2 cells ranged from 2.67% to 8.67%. Moreover, the five strains can decrease the adhesion of E. coli to Caco-2 cells by 36.67–98.73% through competition inhibition, exclusion inhibition, and replacement inhibition. Finally, the five probiotics were sensitive to some of the antibiotics examined and were innocuous with nonhemolytic activity. The preliminary works indicate that the five candidates could show a good potential application against hyperuricemia.
研究领域:
微生物学
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生物技术
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营养学
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药理学
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产品关联
DOI
高影响因子
Journal of Zhejiang University-SCIENCE B
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2016
·Vol. 17
·期 8
·597-609
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IF 1.303
Azat Ramila,Liu Yan,Li Wei,Kayir Abdurihim,Lin Ding-bo,Zhou Wen-wen,Zheng Xiao-dong
Six lactic acid bacterial (LAB) strains were isolated from traditionally fermented Xinjiang cheese and evaluated for functional and probiotic properties and potentials as starter cultures. The isolated six LAB strains comprised Lactobacillus rhamnosus (one strain), Lactobacillus helveticus (one strain), and Enterococcus hirae (four strains). All of the six strains were tolerant to acidic and bile salt conditions. Among which, the L. rhamnosus R4 strain showed more desirable antimicrobial, auto-aggregation, and hydrophobic activity. In addition, the strain L. rhamnosus R4 exhibited the highest level of free radical scavenging activity (53.78% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals and 45.79% of hydroxyl radicals). L. rhamnosus R4 also demonstrated cholesterol and triglyceride degradation by 50.97% and 28.92%, respectively. To further examine the health-promoting effects of these LAB strains on host lifespan, Caenorhabditis elegans was used as an in vivo model. Worms fed LAB as a food source had significant differences in lifespan compared to those fed Escherichia coli OP50 (as a negative control). Feeding of L. rhamnosus R4 extended the mean lifespan of C. elegans by up to 36.1% compared to that of the control. The results suggest that the strains isolated from Xinjiang fermented dairy products have high potential as starter cultures in the cheese industry.
研究领域:
食品科学
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微生物学
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营养学
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功能性食品
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）