MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Su Han Wang,Min Lin,Jing Liu,Jian Ping Wang,Ning Peng Wu
The quinolone efflux pump (QepA) protein of a fluoroquinolones-resistant Escherichia coli was grafted on Fe 3 O 4 to synthesize a type of magnetic composite Fe 3 O 4 @QepA. This material was combined with 4 fluorescent tracers to develop a semi-homogeneous fluorescent array on 96-well microplate for determination of fluoroquinolones (FQs) in chicken, and the results were analyzed by using linear discriminant analysis method. Results showed this method could discriminatively determine 15 FQs at the concentration ≥10 ng/g, and could identify each drug species at the concentration ≥2 ng/g. The method exhibited satisfactory recoveries in chicken sample (61.7 %–119.8 %). The analysis results for some unknown samples were consistent with a liquid chromatography mass spectrometry method. This is the first paper reporting a semi-homogeneous fluorescent array for discriminative determination of small molecule substances, and this array can be used as an accurate tool to determine the 15 FQs in meat sample.
研究领域:
分析化学
·
微生物学
·
食品安全
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产品关联
DOI
高影响因子
ANALYST
·
2022
·Vol. 147
·期 22
·5011-5017
·
IF 5.227
Jiaxi Yin,Hairong Cui,Ling Lei,Kangbing Wu
Graphene nanosheets (GS) were prepared by ultrasonic exfoliation of bulk graphite in N-methyl-2-pyrrolidone with the assistance of sodium pyrophosphate. The obtained GS suspension was modified on a glassy carbon electrode (GS/GCE), and then functionalized at different voltages (e.g. 1.0, 1.4 and 1.6 V) for 2 min in pH 7.0 phosphate buffer. The electrochemically functionalized GS/GCE (i.e. EGS/GCE) possesses more oxygen-containing groups and a higher defect level. More importantly, the active response area, electron transfer ability and interface adsorption capacity of the EGS/GCE enhanced remarkably. The possible mechanism of the performance enhancement is discussed, and the sensing application of the EGS/GCE in the detection of nitrofurazone (NFZ) is investigated. Compared with the GS/GCE, the EGS/GCE is much more active for NFZ oxidation and greatly increases the detection sensitivity. As a result, a highly sensitive electrochemical detection method has been developed for NFZ, with a detection limit of 2.1 nM. The practical application of the EGS/GCE was tested in fish meat samples, showing good accuracy and feasibility.
研究领域:
分析化学
·
材料科学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2020
·Vol. 13
·期 10
·1938-1946
·
IF 2.667
Pang Yue-Hong,Sun Miao,Shen Xiao-Fang,Yue Qi,Ma Tian-Tian,Yang Cheng
Benzo[a]pyrene (BaP) is a kind of trace pollutant commonly found in environment, food, and organism. It has been widely concerned because of its strong carcinogenicity to the human body. In this paper, covalent organic framework TpBD, in situ growth on titanium wire, was used as the coating of solid-phase microextraction (SPME) fiber and coupled with gas chromatography–flame ionization detector (GC-FID) for the detection of trace BaP in roast meat samples. The linear range of the developed method under the optimized experimental conditions was 0.02–15 μg L −1 , the detection limit (S/ N = 3) was 0.006 μg L −1 , and the limit of quantification (S/ N = 10) was 0.02 μg L −1 . The intra-day and inter-day precision of a single fiber were 1.73% and 2.27%, respectively. The relative standard deviation (RSD) between fiber-to-fiber was investigated by three as-fabricated fibers was 6.09%. Moreover, the enrichment factor of the fabricated TpBD SPME fiber for BaP was 4914, and the fiber can withstand at least 150 cycles of adsorption and desorption without significantly decrease in extraction efficiency (< 3.6%). Finally, the developed method was successfully applied to the detection of BaP in roast meat samples with the recoveries from 95.4 to 108.5%.
研究领域:
分析化学
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食品安全
·
环境科学
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产品关联
DOI
高影响因子
ELECTROPHORESIS
·
2020
·Vol. 42
·期 4
·465-472
·
IF 3.081
Qiuyue Xu,Beibei Chen,Man He,Bin Hu
Ti (IV)-modified vinyl phosphate magnetic nanoparticles (Fe 3 O 4 @SiO 2 @KH570-PO 4 -Ti (IV)) was prepared for simultaneous extraction of multiple arsenic species, followed by high performance liquid chromatography (HPLC)– inductively coupled plasma mass spectrometry (ICP-MS) analysis. Inorganic arsenic (iAs), dimethyl arsenic acid (DMA), monomethyl arsenic acid (MMA), p -amino phenyl arsenic acid ( p -ASA), 4-hdroxyphenylarsenic acid (4-OH), phenyl arsenic acid (PAA), and 3-nitro-4-hydroxyphenylarsenic acid (ROX) were investigated as interest analytes. It was found that they were quantitatively adsorbed on Fe 3 O 4 @SiO 2 @KH570-PO 4 -Ti (IV) at pH 5, and desorbed completely with 0.1 mol/L sodium hydroxide solution. Enrichment factor of 100-fold was obtained by consuming 100 mL sample solution. Under the optimal conditions, the method combining MSPE with HPLC-ICP-MS presented a linear range of 1–5000 ng/L for seven arsenic species. The limits of detection were 0.39, 0.60, 0.23, 1.85, 0.54, 0.48, and 0.84 ng/L for DMA, MMA, p -ASA, iAs, 4-OH, PAA, ROX, with the relative standard deviations ( c = 10 ng/L, n = 7) of 3.6, 3.9, 5.5, 12.4, 6.1, 5.8, 5.0, respectively. The accuracy of the method was validated by analyzing BCR 627 Tuna fish. The application potential of the method was further evaluated by chicken muscle and liver samples. No target arsenic species were detected in these samples, and good recoveries (80.6–123%) were obtained for the spiked samples at low, medium, and high concentration levels.
研究领域:
分析化学
·
环境科学
·
食品科学
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Tian-Tian Ma,Xiao-Fang Shen,Cheng Yang,Hai-Long Qian,Yue-Hong Pang,Xiu-Ping Yan
Covalent organic framework TpBD was grafted on stainless steel wire with polydopamine as a linker. The fabricated TpBD bonded stainless steel wire was used as the solid-phase microextraction fiber to extract sixteen polycyclic aromatic hydrocarbons (PAHs) for subsequent GC-MS/MS determination in grilled meat samples. The developed method gave the limits of detection ( S/N = 3) from 0.02 (pyrene)-1.66 (naphthalene) ng L −1 and enhancement factors from 1069 (naphthalene)-10879 (benz(a)anthracene). The relative standard deviations (RSDs) for intra-day and inter-day study are in the range of 2.6%–8.5% and 4.5%–9.4%, respectively. The fiber-to-fiber RSDs for three parallel prepared fibers were 5.3%–10.0%. One TpBD bonded fiber can stand at least 200 cycles without significant loss of extraction efficiency. The developed method was successfully applied for the determination of trace PAHs in grilled meat samples with recoveries from 85.1% to 102.8%.
研究领域:
分析化学
·
食品科学
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DOI
高影响因子
ANALYST
·
2020
·Vol. 145
·期 14
·5011-5018
·
IF 3.978
Xinyang Zhang,Jianan Wen,Lili Lian,Xianhong Ma,Xiyue Wang,Dawei Lou
3D magnetic porous carbon (MPCK) was prepared using the metal–organic framework (MOF) MIL-100(Fe) as the carbon precursor by carbonisation and KOH activation strategies. Carbonisation and activation ensured that MPCK possessed excellent structural and thermal stability, strong magnetic responsiveness and high surface area. MPCK was used as an adsorbent for the magnetic solid-phase extraction of clenbuterol and ractopamine from mutton samples. The concentration levels of analytes were determined by ultra-high performance liquid chromatography-mass spectrometry. Under optimised extraction conditions, the peak area responded linearly to analytes over the concentration range from 0.05 μg L−1 to 40 μg L−1 (r ≥ 0.9972). The detection limits of clenbuterol and ractopamine were found to be 0.130 μg kg−1 and 0.150 μg kg−1, respectively. The satisfactory recoveries in mutton samples ranging from 95.64% to 114.65% indicated that 3D porous carbon is a promising adsorption material for the extraction of clenbuterol and ractopamine from complex biological matrixes.
研究领域:
分析化学
·
食品科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2020
·
IF 5.065
Wei Zhang,Peipei Zhou,Wei Liu,Huili Wang,Xuedong Wang
Owing to matrix complexity, the development of green and convenient pretreatment methods is crucial for routine monitoring of organic contaminants in meat samples. Dicationic ionic liquids (DILs) possess two active centers, thus resulting in superior physicochemical properties over monocationic species. Herein, the hydrophilic DILs and in-situ metathesis reaction salt were introduced into NiFe 2 O 4 -based magnetic effervescent tablets for enhancing adsorption/extraction of five typical polycyclic aromatic hydrocarbons (PAHs) in meats. Also, the synthesized NiFe 2 O 4 nanoparticles displayed larger specific surface area than others (CoFe 2 O 4 , Fe 3 O 4 and ZnFe 2 O 4 ), thereby supplying higher adsorption capacity for PAHs. The integration of DILs with NiFe 2 O 4 into magnetic effervescent tablets realized highly efficient adsorption/extraction, rapid magnetic separation/retrieval and CO 2 -induced vigorous dispersion into one synchronous step. After hydrolysis of the ground meat samples using 10 μM KOH, the filtered solution was diluted and subjected to magnetic effervescent tablet-assisted microextraction procedures. Optimized experimental variables were as follows: an effervescent tablet containing 0.7 g Na 2 CO 3 and NaH 2 PO 4 with molar ratio of 1:2; 60 mg [C 4 (MIM) 2 ]Br 2 ; 20 mg NiFe 2 O 4 ; 45 mg Na[N(CN) 2 ] and 500 μL toluene as elution solvent. The effervescent reaction-enhanced microextraction was combined with high performance liquid chromatography and fluorescence detection, rendering limits of detection as low as 0.01–0.07 μg kg −1 with high recoveries (82.3–104.7%) for five PAHs in raw, boiled, charcoal-grilled and smoked meats. To the best of our knowledge, this is the first report on the additive action between DILs and NiFe 2 O 4 in an effervescent reaction-enhanced microextraction, showing great analytical potential in meat matrices.
研究领域:
分析化学
·
食品科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Juan Wang,Zhiyan Chen,Zhiming Li,Yaling Yang
A simple and rapid magnetic nanoparticles (MNPs) based dispersive micro-solid-phase extraction (D-μ-SPE) method coupled with HPLC-DAD has been proposed for simultaneous determination of three estrogens (17β-estradiol (E2), estrone (E1) and diethylstilbestrol (DES)) in pork samples. In this paper, the synthesis of cetyltrimethyl ammonium bromide (CTAB)-coated Fe 3 O 4 @caprylic acid NPs as an efficient sorbent for its high surface area, excellent adsorption capacity, good dispersion ability and high super-paramagnetic property was successfully applied to adsorb estrogens. Vortex was used to enhance mass transfer rate as it provided mild and effective mixing of sample solution and increased the contact between analytes and MNPs. The parameters affecting the extraction efficiency were investigated in detail. The dosages of sorbent and eluate are 100 μL and 500 μL, respectively. The extraction equilibrium was achieved within 2 min and the MNPs can be reused. The proposed technique provided high recoveries (93.3–106.7%), good linearity (0.9993–0.9999), low LODs (0.021–0.033 ng mL −1 ) and repeatability (RSD% = 1.87–2.92).
研究领域:
分析化学
·
食品安全
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 33
·16177-16182
·
IF 3.925
Shuyan Xiao,Liang Sun,Jiping Lu,Zhongping Dong
A label-free fluorescent biosensor based on the clenbuterol binding aptamer and the fluorescent intercalator SYBR Green I (SGI) was established for the sensitive and selective detection of clenbuterol. In the absence of clenbuterol, a weak fluorescence signal of SGI was observed. In the presence of clenbuterol, the specific recognition and high-affinity binding of the aptamer with clenbuterol induced a conformational change of the aptamer, and a longer double-stranded DNA was formed. As a result, an enhancement in the fluorescence of SGI could be found. Under optimal conditions, the fluorescence change ratio increased linearly with clenbuterol concentration from 50 nM to 450 nM, with a detection limit of 18.8 nM. In addition, the proposed aptasensor showed good selectivity and was successfully applied for clenbuterol quantification in pork samples with recoveries of 90.37–104.98%.
研究领域:
分析化学
·
食品安全
·
分子生物学
✓ 已验证
DOI
高影响因子
Food Analytical Methods
·
2024
·
IF 2.6
Yang Wenming,Liu Chenghao,Zhang Baolin,Wu Changchun,Cao Yang,Huang Weihong,Xu Wanzhen
In the realm of food safety, the accumulation and potential health risks posed by persistent residues of broad-spectrum antimicrobials, which degrade slowly in the environment, have prompted the urgent development of efficient and sensitive detection methods. Addressing this challenge, nitrogen-doped carbon quantum dots (N-CQDs) were synthesized through a high-temperature hydrothermal method using citric acid and urea as sources. Utilizing these N-CQDs, a sensor incorporating molecularly imprinted polymers was developed through a sol–gel process with sulfadiazine (SDZ) as the template molecule. This involved using 3-aminopropyltriethoxysilane and tetraethyl orthosilicate as the monomer and cross-linker, respectively. The resulting sensor exhibited a linear detection range of 0 to 30 µmol/L and a notably low detection limit of 0.04 µmol/L. Further testing on actual pork samples revealed recovery rates between 95.10 and 108.30% and a relative standard deviation below 2.0%, emphasizing its high sensitivity and selectivity. This advancement not only enhances SDZ detection capabilities, but also paves the way for broader applications in food safety monitoring, setting a solid foundation for future developments in sensor technology. Graphical Abstract
研究领域:
食品安全
·
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2021
·Vol. 44
·期 24
·4313-4326
·
IF 3.645
Tingting Liu,Evans AgyeKum,Sai Ma,Hanzhang Ye,Jiani Li,Ming Gao,Min Ni,Xiaofan Zhang,Xuedong Wang
To simultaneously and efficiently extract pollutants with differential polarities, we herein fabricated and characterized a multifunctional nanocomposite. The novel nanohybrids used NiFe 2 O 4 as magnetic cores, and NH 2 -MIL-101(Al), β-cyclodextrin and graphene oxide as functional components combined with magnetic cores. With the aid of graphene oxide's large π-conjugated system, NH 2 -MIL-101(Al)’s strong adsorption to moderately/strongly polar chemicals, and β-cyclodextrin's specific recognition effect, the nanohybrids realized synergistically efficient extraction of polyaromatic hydrocarbons and bisphenols with a log Kow range of 3–6. Combined with acidic and alkaline sources, the nanohybrids-based effervescent tablets were prepared. Based on effervescent reaction-enhanced nanohybrids-based efficient adsorption/extraction and high performance liquid chromatography and fluorescence detection, we successfully developed an excellent microextraction method for the simultaneous determination of both polyaromatic hydrocarbons and bisphenols in roasted meat samples. Several important variables were optimized as follows: Na 2 CO 3 and tartaric acid as acidic and alkaline sources, 900 μLof the mixed solvent (acetone and hexane at 2:1 by v/v) as the eluent, 5 min of elution time. Under optimized conditions, the novel method gave low limits of detection (0.07–0.30 μg kg −1 ), satisfactory recoveries (86.9–103.9%), and high precision (relative standard deviations of 1.9–6.7%) in roasted lamb, beef, pork, chicken, and sausage samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Xiuzhen Qiu,Xian-Yan Xu,Yong Liang,Yongbiao Hua,Huishi Guo
In this paper, a method for the synthesis of ractopamine molecularly imprinted polymers (MIPs) nanotube membranes on anodic alumina oxide (AAO) nanopore surface by atom transfer radical polymerization (ATRP) was presented, in which methacrylic acid (MAA) was selected as functional monomer with a polymerization rate of 1:6 between ractopamine and MAA by the computational investigations. The morphology of MIPs nanotube membranes characterized by scanning electron microscope (SEM) suggested a well growth in the AAO nanopore surface. A series of adsorption experiments revealed that the MIPs nanotube membranes showed better extraction capacity and good selectivity for ractopamine and its analogues than that of non-imprinted polymers (NIPs) nanotube membranes. In order to evaluate the usability of the MIPs nanotube membranes, a methodology by combining MIPs nanotube membranes extraction couple with high performance liquid chromatography (HPLC) detection for the determination of β 2 -agonists in complex samples was developed. The linear ranges were 10–1000 μg/L for ractopamine, 100–1000 μg/L for clenbuterol, epinephrine and dopamine, and 200–1000 μg/L for terbutaline. The detection limits were within the range of 0.074–0.25 μg/L and the RSDs ( n = 3) were from 2.8% to 4.3%. The method was successfully applied to the analysis of β 2 -agonists in spiked real samples, The recoveries of all the β 2 -agonists at the two concentration levels were found to be within the range of 86.3–97.0% and 82.8–95.7%, respectively. The RSDs were within 2.7–5.7%. The results demonstrated that the proposed method is very suitable for the determination of β 2 -agonists in pork samples.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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