Analytical Methods
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2012
·Vol. 5
·期 1
·59-63
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IF 1.547
Suo Decheng,Li Lan,Zhang Su,Su Xiaoou
A simple, highly sensitive ion chromatographic method for the simultaneous determination of betaine, choline and carnitine in feed premixes is described. Premixes were extracted via ultrasonication in water for 20 min and extracts were analyzed by ion chromatography using an aqueous 3.0 mmol L−1 methane sulfonic acid solution containing 15% (v/v) acetonitrile as the eluent and an IonPac SCS1 column. The composition of the mobile phase was optimized for the efficient separation of the three main analytes from each other as well as from common inorganic cations and trimethylamine, which is an ingredient for the synthesis of the analytes. The recoveries of choline, carnitine and betaine spikes added to premixes were all greater than 90% with replicate relative standard deviations of less than 10%. The limits of detection of this method for these analytes in premixes were calculated to be 10, 6 and 20 mg kg−1, respectively.
研究领域:
分析化学
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营养学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 18
·8912-8918
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IF 3.925
Qianqian Hu,Guoning Chen,Lu Wang,Xia Cui,Chun Chang,Qiang Fu
Sarcosine is an important biomarker in the diagnosis of prostate cancer. It is essential to explore a method that detects sarcosine in biological samples quickly and accurately for the early screening and diagnosis of prostate cancer. Considering the high catalytic efficiency and good selectivity of enzymes, there is potential for establishing enzymatic methods for the determination of sarcosine. However, the poor stability, high economic consumption, and challenging recyclability of free enzymes limit their use. Here, sarcosine oxidase was immobilized to improve its stability. The properties of the immobilized enzyme, including the morphology and stability, were characterized. Then, the enzymatic reaction between the immobilized enzyme and the substrate sarcosine was employed to establish a fluorescence method with a linear range from 0.1563 to 10.00 μg mL−1, a LOD of 0.04 μg mL−1, and a recovery of 84.7–104.0%. It has been proved that sarcosine can be detected using the established method with short time consumption, high specificity, and low cost, and which is easy to operate and is affordable for many laboratories and community-level medical institutions. These results demonstrate that this method shows potential for practical applications in the diagnosis of prostate cancer.
研究领域:
生物化学
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医学诊断
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材料科学
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DOI
高影响因子
Marine Drugs
·
2021
·Vol. 19
·期 2
·63
·
IF 5.118
Guo-Fang Duan,Yang Liu,Li-Na Zhang,Hong-Ye Li,Jie-Sheng Liu,Wei-Dong Yang
Diarrhetic shellfish toxins (DSTs), some of the most important phycotoxins, are distributed almost all over the world, posing a great threat to human health through the food chain. Therefore, it is of great significance to find effective methods to reduce toxin accumulation in shellfish. In this paper, we observed the effects of four phytochemicals including cinnamaldehyde (CA), quercetin, oridonin and allicin on the accumulation of DSTs in the digestive gland ofPerna viridisafter exposure to the DSTs-producingProrocentrum lima. We found that, among the four phytochemicals, CA could effectively decrease the accumulation of DSTs (okadaic acid-eq) in the digestive gland ofP. viridis. Further evidence demonstrated that CA could reduce the histological alterations of the digestive gland of a mussel caused by DSTs. RT-qPCR showed that CA could suppress the CYP3A4 induction by DSTs, suggesting that the DSTs’ decrease induced by CA might be related to the inhibition of CYP3A4 transcription induction. However, further studies on the underlying mechanism, optimal treatment time, ecological safety and cost should be addressed before cinnamaldehyde is used to decrease the accumulation of DSTs in field.Keywords:okadaic acid;Perna viridis;cinnamaldehyde;CYP3A4
研究领域:
海洋生物学
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毒理学
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药理学
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DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Xin-Nan Wang,Jian-Qun Liu,Wen-Liang Ji,Zong-Li Huo,Li-Fang Liu,Jia-Yi Zheng
Trimethylamine (TMA) metabolism comprises choline-containing compounds’ metabolization, TMA production and trimethylamine N-oxide (TMAO) generation. However, the presence of numerous compounds in the carnitine and phosphatidylcholine (PC) pool compositions complicates profiling work significantly. This study is aimed at developing an efficient method for profiling TMA metabolic pathways, including quantifying known compounds and semi-quantifying the differential metabolites in the carnitine and PC pool compositions. Pseudo-targeted metabolomics is applicable for characterization. Firstly, multivariate statistics were performed to identify valuable metabolites (variable importance in the projection ＞1) from quality control biological samples. Given that TMA metabolism involved in host-gut microbiota interaction, co-metabolites were defined as the intersections of valuable metabolites from different biological samples (serum, liver, and intestinal contents) and further screened. Finally, alterations in TMA metabolism were observed in dextran sulfate sodium-induced colitis, with semi-quantitative analysis for excavated co-metabolites including 11 PCs, 6 lyso-phosphatidylcholines, and 2 acylcarnitines and quantitative analysis for 10 known metabolites. The findings revealed increased TMA production and accumulation of choline-containing compounds in the gut during ulcerative colitis exacerbation. Correspondingly, the circulating level of TMAO was elevated in the colitis group. A comprehensive understanding of TMA metabolism can contribute to disease differential diagnoses and potential mechanism studies.
研究领域:
医学
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微生物学
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代谢组学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2019
·
IF 9.518
Qia Wang,Yuting Zhao,Qingui Yang,Dan Du,Haipeng Yang,Yuehe Lin
Amperometric enzyme biosensors are some of the simplest and cheapest types of medical devices used in the rapid detection of biomarkers that have been developed in the past fifty years. When the concentrations of biomarkers are at micromoles per liter, such as for sarcosine , which was recently discovered as a biomarker for prostate cancer, the response signal of the interferences is huge, and the biosensor is hard to satisfy the requirements of practical applications. In this manuscript, we describe a strategy for synthesizing a surface electronegative organic-inorganic hybrid mesoporous material , which could reduce the interference signal much better than Nafion and Chitosan. We verify that the surface potential of the carrier nanomaterial plays an important role in excluding anionic interferences. We also prepare a sensitive (16.35 μA mM −1 ), low LOD (0.13 μM) and wide linear range (1–70 μM) amperometric sarcosine biosensor with excellent anti-interference properties. This mesoporous material provides a bio-composite platform for the development of simple amperometric biosensors for detecting micromoles per liter of analytes in serum or urine.
研究领域:
生物医学工程
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分析化学
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癌症诊断
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 18
·3444-3451
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IF 7.571
Hao Shen,Haimei Shi,Bin Feng,Chuanfan Ding,Shaoning Yu
The combination of bio- and chemo-catalysts for sequential cascades has received considerable attention in analytical fields because of the regulable catalytic efficiency and selectivity under various physiological conditions. In this paper, a versatile multienzyme cascade nanoplatform with excellent activity for biosensing is demonstrated by combining metal–organic framework (MOF)-based nanozyme with natural enzymes. A boronic acid-modified MOF, MIL-100(Fe)-BA, was obtained via a microwave-assisted metal–ligand-fragment co-assembly strategy. On the one hand, MIL-100(Fe)-BA could serve as a nanozyme with dual oxidase/peroxidase bioactivity to detect glutathione and ascorbic acid with a detection limit of 0.12 μM and 0.09 μM, respectively. On the other hand, the hierarchically porous MIL-100(Fe)-BA possesses adequate recognition sites for immobilizing enzymes with acceptable protein leakage, enabling it to act like a scaffold for the fixation of a single enzyme (sarcosine oxidase) or bi-enzymes (acetylcholinesterase/choline oxidase) and guide a multienzyme cascade reaction system with high efficiency. The cascade nanoplatform has merits of both artificial nanozymes and natural enzymes, providing satisfactory sarcosine/acetylcholine sensing ability with detection limits of 0.26 μM and 1.18 μM. The developed catalytic system not only expands the application of nanozymes in tandem enzymatic bio-catalysis, but provides a facile and efficient multienzyme cascade nanoplatform for biosensing and other applications.
研究领域:
生物化学
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 40
·19442-19454
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IF 3.925
Zhi-Gang Yin,Xiong-Wei Liu,Lin Chen,Xiong-Li Liu,Bo-Wen Pan,Ying Zhou
A substrate-dependent, highly β-regioselective one-pot multicomponent 1,3-dipolar cycloaddition reaction of 3-methyl-4-nitro-5-isatylidenyl-isoxazoles with azomethine ylides (thermally generated in situ from ninhydrin and sarcosine) is reported for the first time, furnishing a library of biologically useful bispiro[oxindole-isoxazole-indandione] hybrids. Target novel and structurally complex compounds have been obtained in good yields and diastereoselectivities (up to 91% yield and 20 : 1 dr) with two adjacent spiro quaternary centres. Furthermore, the described method, which is different from our previous work of α-regioselective cycloaddition of 3-methyl-4-nitro-5-isatylidenyl-isoxazoles, is a highly β-regioselective cycloaddition. The newly synthesized compounds potently inhibited the proliferation of various cancer cell lines. The most potent compound 4ah could induce the apoptosis of K562 cells through a caspase-dependent and -independent pathway.
研究领域:
有机化学
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药物化学
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癌症研究
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）