ACS Omega
·
2021
·
IF 3.512
Hangjie Wu,Lide Gu,Xiaoyi Ma,Xueqing Tian,Shihui Fan,Mingcan Qin,Jing Lu,Mingsheng Lyu,Shujun Wang
Helicobacter pylori was first isolated from gastritis patients by Barry J. Marshall and J. Robin Warren in 1982, and more than 90% of duodenal ulcers and about 80% of gastric ulcers are caused by H. pylori infection. Most detection methods require sophisticated instruments and professional operators, making detection slow and expensive. Therefore, it is critical to develop a simple, fast, highly specific, and practical strategy for the detection of H. pylori. In this study, we used H. pylori as a target to select unique aptamers that can be used for the detection of H. pylori. In our study, we used random ssDNA as an initial library to screen nucleic acid aptamers for H. pylori. We used binding rate and the fluorescence intensity to identify candidate aptamers. One DNA aptamer, named HPA-2, was discovered through six rounds of positive selection and three rounds of negative selection, and it had the highest affinity constant of all aptamers tested (Kd = 19.3 ± 3.2 nM). This aptamer could be used to detect H. pylori and showed no specificity for other bacteria. Moreover, we developed a new sensor to detect H. pylori with the naked eye for 5 min using illumination from a hand-held flashlight. Our study provides a framework for the development of other aptamer-based methods for the rapid detection of pathogenic bacteria.
研究领域:
微生物学
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生物技术
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分子生物学
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PubMed
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 5
·期 4
·826-833
·
IF 4.872
Zhenzhen Lin,Shuzhen Lv,Kangyao Zhang,Dianping Tang
This work designs a simple low-cost visual fluorescence immunoassay for disease-related biomarkers (carcinoembryonic antigen, CEA, used as a model protein) in biological fluids, based on the structural and optical transformation of CdTe quantum dots (QDs) immobilized on paper induced by dissolved silver ions (Ag+) from silver nanoparticles (AgNPs) via a cation-exchange reaction. A sandwich-type immunoreaction was initially carried out in a removable polystyrene high-binding microplate coated with monoclonal anti-CEA capture antibody by using AgNP-labeled polyclonal anti-CEA antibody as the detection antibody. Thereafter, the carried AgNPs accompanying the sandwiched immunocomplexes were dissolved by acid to release numerous silver ions, which induced the ion-exchange reaction with the immobilized CdTe QDs on the paper (attached onto the microplate lid) for the Cd-to-Ag transformation, thus resulting in the quenching of the visual fluorescence from the CdTe QDs owing to Ag2Te formation. Under optimal conditions, the fluorescence intensity decreased with the increasing CEA concentration from 0.02 to 50 ng mL−1 with a detection limit of 5.6 pg mL−1. Further, a visual assay based on a CdTe QD-based paper sensor was developed for CEA detection, and 5.0 pg mL−1 CEA could be discriminated with the naked eye. In addition, our strategy displayed high specificity, good reproducibility and acceptable accuracy for analyzing human serum specimens with consistent results obtained using the commercialized enzyme-linked immunosorbent assay (ELISA) method.
研究领域:
分析化学
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生物传感器
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生物医学工程
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分子诊断
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2018
·
IF 2.746
Rui Dai,Wenqing Deng,Pingyue Hu,Chao You,Lu Yang,Xue Jiang,Xiaoli Xiong,Ke Huang
A novel, rapid and ultrasensitive ratiometric and colorimetric fluorescence method was developed using bovine serum albumin protected gold/silver bimetallic nanoclusters (BSA-Au/Ag NCs) as fluorescent sensor for mercury (Hg) detection. The fluorescence sensor which was synthesized by one-pot approach shows dual-emissions at 470 nm (Ag NCs) and 630 nm (Au NCs) under a single excitation at 390 nm. In the presence of Hg 2+ , the red fluorescence of Au NCs was quenched while the fluorescence of Ag NCs almost unchanged, which exhibited a ratiometric fluorescence response and an obvious fluorescence color change toward Hg 2+ . The selective quenching mechanism may be due to the formation of gold amalgam bonds between Hg 2+ (4f 14 5d 10 ) and Au + (4f 14 5d 10 ). A limit of detection (LOD) of 2.2 μg L −1 and a relative standard deviation (RSD, n = 7) of 0.7% at a concentration of 50 μg L −1 of Hg 2+ were obtained when using a commercial fluorescence spectrophotometer as the detector. In visual detection, as low as 10 μg L −1 of Hg 2+ can be easily discriminated from the blank with the naked eye. The proposed method retains several unique advantages, including simplicity, rapidity, ease of usage and high-sensitively.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 2
·1-9
·
IF 5.3
Su Yuan,Zhang Ziyu,Zhang Hongfang
A biosensor based on solid-state nanochannels of anodic aluminum oxide (AAO) membrane for both electrochemical and naked-eye detection of microRNA-31 (MiR-31) is proposed. For this purpose, MoS 2 nanosheets, which possess different adsorption capabilities to single-stranded and double-stranded nucleic acids, are deposited onto the top surface of the AAO membrane. Moreover, multi-functional DNA nanostructure have been designed by linking a G-rich sequence for folding to a G- quadruplex at three vertices and a complementary sequence of MiR-31 at the other one vertex of a DNA tetrahedron. In the absence of MiR-31, the tetrahedron DNAzyme probe formed after the addition of hemin can mediate the deposition of insoluble on MoS 2 /AAO, which not only enables the color change of the membrane but also gates the transport of K 3 [Fe(CN) 6 ] across the nanochannels. Therefore, the detection of MiR-31 is realized by both visual observation of the brown color and measuring the electrochemical redox current of [Fe(CN) 6 ] 3 − . Using this biosensor, a detection limit as low as 0.06 fM is achieved. The dual-mode detection method also exhibits good specificity, reproducibility, and stability, demonstrating potential application in the diagnosis of oral squamous cell carcinoma and other related biological purposes. Graphical
研究领域:
生物传感器
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分子诊断
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纳米技术
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癌症研究
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DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2025
·
IF 3.1
Guo Yangyang,Wang Chaoyue,Liu Ying,Zheng Guangjin,Meng Juan
Monitoring the hydrazine (N 2 H 4 ) level is essential for environmental and health safety, while traditional detection methods face challenges including low sensitivity, complex sample handling, and sophisticated operation. In this study, a novel fluorescent probe, ZNP , was designed and synthesized for tracking hydrazine in soils and river water. It was used for in situ monitoring of hydrazine generation in living cells. The optical performance guaranteed the high selectivity and reliable anti-interference. The most significant merit was a practical dynamic range for intuitive visualization. Additionally, the develop probe predominantly accumulated in lysosomes. Notably, ZNP integrated the fluorescent and visual signals, which enabled the naked-eye observation. This work acted as a significant step towards the practical tools for environmental toxicology applications.
研究领域:
环境毒理学
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分析化学
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生物成像
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化学传感
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Fuyuan Huang,Yayun Jiang,Qiaoli Wu,Chaochuan Zheng,Shen Huang,Huan Yang,Guangxin Xiang,Laibao Zheng
Loop-mediated isothermal amplification (LAMP) is a rapid and sensitive nucleic acid testing method for pathogen detection, yet the absence of a straightforward readout strategy remains challenging. We've successfully designed polyethyleneimine-stabilized gold nanoclusters (PEI-AuNCs) as a cationic AuNCs indicator tailored for distinguishing LAMP results, enabling direct visual inspection under UV light. Positive LAMP reactions with PEI-AuNCs, in combination with magnesium pyrophosphate crystals, yield red-fluorescent bulk precipitates visible to the naked eye. To address contamination concerns, we introduced a one-pot reaction by incorporating AuNCs into the lid recess. This one-pot LAMP assay demonstrates exceptional detection capability, identifying Salmonella enterica at concentrations as low as 10 1 CFU/mL within approximately 50 min, excluding nucleic acid extraction. The platform's versatility, achieved through customizable primers, positions it as a promising molecular diagnostic tool for rapid and visual pathogen detection across scientific disciplines.
研究领域:
分子诊断
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纳米技术
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病原体检测
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生物技术
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Ling-Hong Xiong,Xuewen He,Junjie Xia,Hanwu Ma,Fan Yang,Qian Zhang,Dana Huang,Long Chen,Chunli Wu,Xiaomin Zhang,Zheng Zhao,Chengsong Wan,Renli Zhang,Jinquan Cheng
Development of sensitive, convenient, and cost-effective virus detection product is of great significance to meet the growing demand of clinical diagnosis at the early stage of virus infection. Herein, a naked-eye readout of immunoassay by means of virion bridged catalase-mediated in situ reduction of gold ions and growth of nanoparticles, has been successfully proposed for rapid visual detection of Enterovirus 71 (EV71). Through tailoring the morphologies of the produced gold nanoparticles (GNPs) varying between dispersion and aggregation, a distinguishing color changing was ready for observation. This colorimetric detection assay, by further orchestrating the efficient magnetic enrichment and the high catalytic activity of enzyme, is managed to realize highly sensitive detection of EV71 virions with the limit of detection (LOD) down to 0.65 ng/mL. Our proposed method showed a much lower LOD value than the commercial ELISA for EV71 virion detection. Comparing to the current clinical gold standard polymerase chain reaction (PCR) method, our strategy provided the same diagnostic outcomes after testing real clinical samples. Besides, this strategy has no need of complicated sample pretreatment or expensive instruments. Our presented naked-eye immunoassay method holds a promising prospect for the early detection of virus-infectious disease especially in resource-constrained settings.
研究领域:
病毒学
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分析化学
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医学诊断
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Guojun Weng,Xun Shen,Jianjun Li,Jingyuan Wang,Jian Zhu,Junwu Zhao
The etching of gold nanorods/nanobipyramid, or silver-coated nanorods/nanobipyramid inducing plasmon changes represents an efficient strategy to improve the performance of enzyme-linked immunosorbent assay (ELISA). However, the effect of shape on the sensitivity was negligible, especially the thickness of coated silver shell. Here, we propose a plasmonic ELISA for multi-colorimetric detection of CRP based on the etching of Ag-coated Au nanobipyramid (Au [email protected] ). The effect of silver shell thickness on the sensitivity of plasmon peak shifting was investigated by experiments and DDA calculations. The relationship between the Ag shell thickness and the sensitivity of plasmon peak shifting was obtained. Our results reveal that the thickness of coated Ag shell acts as a key factor in the multi-color change of Au [email protected] etching. It is found that Au [email protected] with medium Ag shell thickness and rod-like shape has the higher sensitivity and is suitable for sensing. At the optimized most sensitive Ag shell, the detection limit of proposed plasmonic ELISA for CRP was determined to be 0.09 ng/mL with a spectrometer in the range from 0.09 ng/mL to 25 ng/mL. Importantly, the visual detection limit was 0.78 ng/mL, which allows the differential diagnosis with the naked eye. Compared with traditional ELISA with the monochromatic intensity variations, the multi-color ELISA proposed in this study has a large linear range and rich color variation for high-sensitivity and naked-eye semi-quantitative detection.
研究领域:
纳米技术
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生物传感
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分析化学
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材料科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jiachun Wang,Yibing Huang,Ziyu Kuai,Yanping Zhang,Qi Shen,Pujing Tian,Weihai Nong,Wantong Jiang,Yuting He,Nana Ran,Yinuo Yin,Tiezhu Li,Quan Luo
Accurate determination of pesticide residues is crucial for food safety. A self-calibration method was developed for dual-signal “naked-eye” detection of organophosphorus pesticides (OPs) using bifunctional gold nanozymes (AuNEs). OPs inhibit the cascade reaction of acetylcholinesterase/choline oxidase (AChE/CHO) to reduce hydrogen peroxide (H 2 O 2 ) production, which affects the AuNE-catalyzed color reaction and quenches the fluorescence of AuNEs with the assistance of Fe 2+ . The multi-enzyme cascade system can be integrated into hydrogel sheets for smartphone-assisted digital, quantitative, and onsite detection of OPs in real food samples by analyzing the linear changes in colorimetric and fluorometric signals, which closely match liquid chromatography-mass spectrometry (LC-MS) results. This method displays a lowest ‘naked-eye’ detection concentration of 7 ppb, a wide linear range (0–2000 ng/mL), and particularly the advantage of anti-interference via self-calibration when one of the signals is abnormal.
研究领域:
分析化学
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食品科学
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环境科学
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PubMed
高影响因子
Journal of Materials Chemistry A
·
2014
·Vol. 2
·期 43
·18304-18312
·
IF 0
Yan Li,Lihuan Wang,Xia Yin,Bin Ding,Gang Sun,Tao Ke,Jingyuan Chen,Jianyong Yu
Given the dire consequences of accidental lead ion (Pb2+) exposure, a portable, sensitive and robust analytical approach capable of colorimetric visualization is highly desirable. In this study, we demonstrate a colorimetric strip that relies on a novel polydiacetylene (PDA) embedded polyacrylonitrile nanofibrous membrane (PAN NFM). The Pb2+-chromic PDA is prepared from controlled mixtures of 10,12-pentacosadiynoic acid (PCDA) and PCDA-5EG, a PCDA derivative with a pentaethylene glycol headgroup. Moreover, the Pb2+ complexation ability is manipulated by controlling the molar ratio of PCDA to PCDA-5EG. The PAN NFM acting as a three dimensional matrix is designed for PDA immobilization, thereby improving the stability, portability and sensitivity. Upon exposure to a series of metal ions, only Pb2+ could induce a color change, which clearly showed that our strip could act as a highly selective probe to detect Pb2+. Ultimately, the strip with a naked eye detection limit of 0.48 μM undergoes a brilliant color transition, from blue to red, in a concentration-dependent manner and all the color transitions of strips are quantitatively visualized by employing a chromatic framework. The findings indicate that the strip is particularly promising for visual Pb2+ recognition due to its facile functionalization, simple construction, and versatility to empower colorimetric signaling.
研究领域:
分析化学
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材料科学
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环境科学
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产品关联
DOI
ACS Applied Nano Materials
·
2022
·
IF 6.14
Hui-Jun Fu,Lin Luo,Yu Wang,Cheng-Long Wang,Hong Wang,Yu-Dong Shen,Hong-Tao Lei,Niko Hildebrandt,Zhen-Lin Xu
Acrylamide is a probable human carcinogen with a wide occurrence in commonly consumed food. Because of the lack of specific antibodies against acrylamide, immunoassays usually measure acrylamide derivates, which require long derivatization times at elevated temperatures. One exception is xanthylacrylamide (XAA), which can be derived from acrylamide at room temperature within 30 min. Here, we present the development of a monoclonal antibody against XAA and a competitive enzyme-linked immunosorbent assay (ELISA) based on the multicolor detection of gold nanorods (AuNRs). The biotinylated monoclonal antibodies bound streptavidin alkaline phosphatase, which induced XAA concentration-dependent silver deposition on the AuNRs. The resulting blue shift of the AuNR surface plasmon resonance peak translated into a strong gray-to-orange color change that could be conveniently distinguished by the naked eye or smartphone color detection. The assay showed a broad linear range (0.3–17.2 ng/mL), a low limit of detection (0.06 ng/mL), and a 3.6-fold sensitivity improvement compared to those of conventional tetramethylbenzidine-based ELISA. The analysis of several different food products showed excellent acrylamide concentration recoveries (84–102%) and confirmed its applicability as an effective tool for routine monitoring of acrylamide in food. Rapid and simple color analysis demonstrated a strong potential for implementation into direct consumer product testing.
研究领域:
分析化学
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食品科学
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纳米技术
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生物化学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Yuanhuan Yang,Feng Gao,Ying Liang,Lichao Guo,Yang Pan,Peng Cao,Yue Zhang
The identification of Chinese medicinal herbs occupies a crucial part in the development of the food and drug market. Although molecular identification based on real-time PCR offers good versatility and uniform digital standards compared with traditional methods, such as morphology, the dependence on large-scale equipment hinders spot detection and marketable applications. In this study, we developed a DNA nanoclaw for colorimetric detection and visible on-site identification of Chinese medicines. When specific miRNA is present, the DNAzyme is activated and cleaves the substrate strand, triggering the catalytic hairpin assembly (CHA) reaction and forming branched DNA junctions on AuNP-I. This can then capture AuNP-II through hybridization and facilitate their aggregation, resulting in a noticeable color change that is observable to the naked eye. By harnessing the dual amplification of DNAzyme and CHA, this highly sensitive nanoprobe successfully achieved specific identification of Chinese medicines. This offers a new perspective for on-site testing in the herbal market.
研究领域:
生物技术
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纳米技术
·
药学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
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