Applied Materials Today
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2023
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IF 8.3
Chuang Liu,Ziyang Liu,Kexin Wang,Yuting Sun,Qianying Liu,Xun Sun,Tingting Yan,Qiang Yang,Xinlong Ma,Huan Zhou,Lei Yang
Though acknowledged as the essential material in bone bleeding control with advantages of low cost, excellent malleability and smooth consistency for operation, traditional bone wax is associated with adverse effects such as foreign body reaction, granuloma growth, and bone healing failure. Herein, we presented a novel ready-to-use bone wax (NBW) based on DCPA (CaHPO 4 ) granules (DCPA-G) and modified waxy starch (MWS), proposed to overcome problems of bone wax but preserving its impressive workability in hemorrhage control. Thanks to rheological and adhesive properties of MWS after exposure to water, NBW was found to be cohesive, malleable, water content dependent tissue adhesive and liquid sealing abilities. Moreover, after hardening under humid environment due to the interaction between MWS and DCPA-G, NBW exhibited compressive strength that is comparable to that of cancellous bone. After smeared to defect model, water leakage was avoided by NBW. Besides, this material showed superior hemostasis and osteogenesis ability over traditional bone wax in vitro . Simultaneously, in vivo tests confirmed the efficiency of NBW in bleeding control and bone regeneration, indicating it a promising candidate in bleeding bone treatment.
研究领域:
生物材料
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组织工程
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骨科
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止血
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骨再生
✓ 已验证
DOI
高影响因子
International Journal of Nanomedicine
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2022
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IF 7.033
Yi Guan,Nan Liu,Yan Yu,Qiang Zhou,Meiyu Chang,Yongheng Wang,Sanqiao Yao
Purpose The objective of this study was to evaluate and compare the histopathological implications of silica nanoparticles (Nano-SiO 2 ) and indium-tin oxide nanoparticles (Nano-ITO), in vivo. Methods Male Sprague-Dawley rats were exposed to Nano-SiO 2 (50 mg/kg) and Nano-ITO (6 mg/kg) by a single intratracheal instillation, respectively. Broncho-alveolar lavage fluid (BALF) and lung tissue were obtained at 7, 14, 28, and 56 days post exposure for analysis of BALF inflammatory factors, total protein, and for lung tissue pathology. Histopathological and ultrastructural change in lungs were investigated by hematoxylin and eosin, Masson’s trichrome, sirius red staining, periodic acid Schiff stain, and transmission electron microscopy. The expression of SP-A, collagen type I and III in lung tissue was determined by immunohistochemistry and ELISA. Results The rats in both models exhibited obvious collagen fibrosis and the severity of the lung injury increased with time after exposure to respective dosage increased. Several parameters of pulmonary inflammation and fibrosis significantly increased in both groups, which was reflected by increased LDH activity, total proteins, TNF-α, and IL-6 levels in BALF, and confirmed by histopathological examination. The results also showed that the two models exhibited different features. Exposure to Nano-ITO caused persistent chronic lung inflammation, illustrated by the infiltration of a large amount of enlarged and foamy macrophages and neutrophils into the lung parenchyma. In Nano-SiO 2 exposed rat lung tissue, granulomatous inflammation was most prominent followed by progressive and massive fibrotic nodules. Compared with the Nano-SiO 2 rats, Nano-ITO exposed rats exhibited significantly severe pulmonary alveolar proteinosis (PAP) pathological changes, lower fibrosis, and higher levels of inflammatory biomarkers. However, Nano-SiO 2 exposed rats had greater fibrosis pathological changes and more severe granulomas than Nano-ITO exposed rats. Conclusion This study suggests that the Nano-SiO 2 -induced model has greater value in research into granulomas and fibrosis, while the Nano-ITO-induced model has greater repeatability in area of PAP.
研究领域:
毒理学
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病理学
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纳米技术
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呼吸医学
✓ 已验证
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
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2024
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IF 2.3
Huafeng Yang,Hui Liu,Yuan Zheng,Bo Li,Shujing Wang,Jun Zhang,Jiandong Wang
Cornus officinalis total glycosides (COTG) derived from the traditional Chinese medicine Cornus officinalis, is a natural immunosuppressant and has been extensively studied in immunomodulation and immunosuppression. This study aimed to explore the effects of COTG on granulomatous lobular mastitis (GLM) and its associated mechanisms. Compared to the model group, COTG effectively ameliorated histopathological damage to breast tissue, reduced mammary gland suppuration, and enhanced the blood-milk barrier. Additionally, COTG treatment reduced the total number of T cells and B cells in GLM rats, significantly improving clinical indicators such as P-selectin, E-selectin, and intercellular cell adhesion molecule-1. We also observed downregulation of CD28 and B7 expression levels, an upregulation of cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) expression, and a significant decrease in inflammatory marker levels in the COTG group. COTG exerts an anti-inflammatory effect in GLM by stimulating CTLA-4, inhibiting the B7-CD28 signaling pathway affecting T cell activation, and promoting the blood-milk barrier. These findings suggest that COTG could be a promising therapeutic option for managing GLM, potentially improving patient outcomes by modulating immune responses and reinforcing the blood-milk barrier.
研究领域:
药理学
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免疫学
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病理学
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中医药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）