SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Shuo Li,Yinghao Duan,Sheng Lei,Jiantong Qiao,Gaiping Li,Baoxian Ye
In this work, a brand new nanocomplex (denoted as [email protected] ) is elaborately designed and successfully synthesized meaning the in-situ assembly of porous-morphology Zn-based MOFs (MOF-5) onto the as-prepared PDDA-rGO-Ni (PGN for short) nanocomposites. When this nanocomplex contributes to construct the electrochemical sensor ( [email protected] /GCE), it presents a highly-sensitive response towards echinacoside, and the first measurement for echinacoside by electrochemistry accordingly evolves forward. The crystallization processes are investigated by FT-IR instrument and XRD analysis, as well as micro-morphology of modified materials is visible upon SEM and TEM. Adorably, a novel ratiometric sensor was progressively fabricated by encapsulating methylene blue (MB) molecules into cubic MOF-5 framewoks (MB encased [email protected] /GCE), and apart from assisting in establishing the analysis method with extreme accuracy and favorable practicality, it dose devote a wider linear scale and a lower detection limit than that [email protected] /GCE has given out. Drawn from the developed calibration curve using differential pulse voltammetry (DPV) technique, the peak intensity ratio (I Ech /I MB ) varies linearly with increased echinacoside concentration in the range of 3.0 × 10 −8 – 1.0 × 10 −6 mol L −1 with limit of detection (LOD) reaching 1.0 × 10 −8 mol L −1 (S/N = 3). Additionally, the proposed assay method has been employed to detect echinacoside in real sample sliced cistanche with satisfactory results.
研究领域:
分析化学
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电化学
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纳米材料
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药物分析
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产品关联
DOI
高影响因子
PHYTOMEDICINE
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2024
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IF 6.7
Jie Yao,Haoqiang Wan,Jingmei Zhang,Wanying Shen,Xiaofang Wei,Chenyan Shi,Baoru Ou,Dongyu Liu,Lanlan Ge,Jia Fei,Xiaobin Zeng
Background Studies have shown that phenylethanoid glycosides (PhGs) have multiple pharmacological effects such as anti-inflammatory, hepatoprotective or neuroprotective functions, whereas their anti-tumor effects are rarely studied. Tubuloside B (Tub B) is a PhG isolated from Cistanche deserticola , a traditional Chinese medicine. To date, there is a lack of comprehensive research regarding the biological activity of Tub B. Purpose The subject of the current study was to investigate the anti-hepatocellular carcinoma (HCC) cell activity and the underlying mechanism of Tub B. Methods We evaluated the in vitro anti-migratory effect of Tub B by scratch and transwell assays. RNA-seq was employed to identify the differential genes by Tub B. Besides, the functional mechanism of Tub B was investigated by distinct molecular biology techniques including immunofluorescent staining, quantitative PCR, as well as western blot analysis. Subsequently, we utilized Hep3B cells for in vivo metastasis assays through spleen injection and evaluated the anti-migratory effect of Tub B in hepatocellular carcinoma (HCC). Results Tub B exhibited in vitro and in vivo inhibition of HCC cell migration. Tub B decreased the expression of transcriptional target genes downstream of the Hippo pathway, including CTGF, CYR61, and N-cadherin as determined by RNA-seq. Furthermore, mechanistic studies confirmed that Tub B increased phosphorylation of YAP at S127, which contributes to YAP cytoplasmic localization. Additionally, overexpression of YAP abrogated Tub B-induced inhibition of HCC migration and the mRNA levels of CTGF, CYR61, and N-cadherin . Conclusions Taken together, these results illustrated that Tub B demonstrated great potential in inhibiting migration of HCC, and a portion of its impact can be attributed to the modulation of the Hippo-YAP pathway.
研究领域:
药理学
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肿瘤学
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分子生物学
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中药学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
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2022
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IF 1.61
Wei-Wei Ye,Xiang-Ying Meng,Qian Zhao,Jian-Yang Chen,Cong Liu,Feng Chen,Yong Zhou,Yi Wang
Echinacoside (ECH) is the main compound of Cistanche deserticola, which possesses antioxidant, antitumor, antifatigue, and anti-inflammatory properties. The present study investigated the protective effects of echinacoside on type 2 diabetes mellitus (T2DM)-induced injury in T2DM injury db/db mice model and insulin-resistant LO2 cell model. The results demonstrated that ECH probably alleviated T2DM-induced injury by mediating the AKT pathway, which provided a new direction for the treatment of T2DM-induced injury.
研究领域:
药理学
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糖尿病研究
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2016
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IF 3.169
Yang Li,Ying Peng,Mengyue Wang,Guisheng Zhou,Yulong Zhang,Xiaobo Li
Cistanches Herba is a famous traditional Chinese medicine that has been in use for treating kidney deficiency, impotence, female infertility, morbid leucorrhea, profuse metrorrhagia, and senile constipation. With the exception of studies available for a few single active ingredients such as echinacoside, acteoside, and poliumoside, comprehensive and systematic studies on in vivo metabolism of Cistanches Herba are lacking despite its widespread clinical application. There is no comparative study yet on the metabolites resulting from the traditional usage of Cistanche deserticola and C. tubulosa water extract – two species that are recorded in Chinese Pharmacopoeia. This further restricts research on the therapeutic effect of Cistanches Herba to a great extent. In this study, a robust and unbiased UPLC-Q-TOF-MS combined pattern recognition analysis (orthogonal partial least squared discriminant analysis, OPLS-DA) was employed to rapidly screen prototype components and metabolites of C. deserticola and C. tubulosa water extract in rat urine, feces, and serum. A total of 71 metabolites from C. deserticola including 25 prototype components and 46 metabolites, and 45 metabolites from C. tubulosa including 18 prototype components and 27 metabolites were tentatively identified. Out of these, 10 metabolites were characterized for the first time in these two species. Results of this study indicate that phenylethanoid glycosides (PhGs) are mainly metabolized into degradation products in the gastrointestinal tract of rats. The chemical components cistanoside B, C, D, and E exist only in C. deserticola and release methylated hydroxytyrosol (HT) following metabolism. This factor contributes to the difference between metabolites of C. deserticola and C. tubulosa water extract in rats and is responsible for the differential therapeutic effect that these two species of Cistanches Herba have on the same diseases.
研究领域:
药理学
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分析化学
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中医药学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2021
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IF 6.165
Helin Xu,Xueqin Li,Yanyan Hao,Xiaobin Zhao,Yun Cheng,Jinli Zhang
To achieve simple and efficient separation of acteoside (ACT), extraction ACT from crude extracts of Cistanche tubulosa via ionic liquid (IL) based aqueous two-phase system (ATPS) is investigated. The IL–ATPS comprising [C 4 mim]BF 4 and (NH 4 ) 2 SO 4 exhibits excellent extraction performance, extracting almost all ACT in the [C 4 mim]BF 4 -rich phase. The high polarity of [C 4 mim]BF 4 having multi-hydrogen bond receptors is conducive for extracting ACT in the IL-rich phase, and (NH 4 ) 2 SO 4 not only provides a weak acidic microenvironment for increasing ACT stability in the IL-rich phase but also reduces the solubility of ACT in the salt-rich phase via the salting-out effect. In addition, molecular simulation results show that ACT is extracted in the [C 4 mim]BF 4 -rich phase via multi-interactions, including hydrogen bonding, van der Waals forces, and π–π stacking. This study is expected to provide a valuable reference for the separation of bioactive constituents from natural products.
研究领域:
植物化学
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天然产物分离
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产品关联
DOI
高影响因子
ANALYTICAL LETTERS
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2014
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IF 1.019
Zhiguo Ma,Zhenya Yang,Danyi Lu,Long Dai,Baojian Wu,Shun Yao
Cistanche deserticola (Orobanchaceae) has been widely used in China for food and medicinal purposes. Chemical differentiation between the raw and steamed C. deserticola was performed by high-performance liquid chromatography coupled with diode array detection and mass spectrometry. Eight chemicals (echinacoside, cistanoside A, acteoside, cistanoside C, 2′-acetylacteoside, isoacteoside, isocistanoside C, and tubuloside B) were obtained from raw and steamed C. deserticola, and the kinetics in the steaming process were investigated in detail. When the steaming time increased, the concentrations of echinacoside, cistanoside A, acteoside, cistanoside C, and 2′-acetylacteoside decreased, while the levels of isoacteoside, isocistanoside C, and tubuloside B increased. Furthermore, two compounds, 5-hydroxymaltol and 5-hydroxymethylfurfural, were found only in the steamed form. In addition, hierarchical clustering analysis and principal component analysis were performed to evaluate the classify the chemical concentrations among steamed C. deserticola. The results showed that steaming had a significant influence on the chemical constitution. The study provide chemical analysis of raw and steamed C. deserticola, including possible transformation pathways, and should allow better understanding of the steaming process of this herb.
研究领域:
中药学
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分析化学
✓ 已验证
DOI
高影响因子
PHYTOCHEMICAL ANALYSIS
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2025
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IF 2.6
Tao-Tao Xue,Dong-Xuan Zheng,Qiang Hou,Li-Mei Wen,Bao-Juan Wang,Ruo-Yu Geng,Qian-Qian Wang,Wu Dai,Li-Ying Tian,Sheng-Qi He,Jian-Hua Yang,Jun-Ping Hu
Introduction Cistanche deserticola Ma (CD), an edible and medicinal plant native to Xinjiang, Inner Mongolia, and Gansu in China, is rich in bioactive polysaccharides known for their health-promoting properties. The polysaccharides of C. deserticola (CDPs) have been shown to possess a range of beneficial activities, including immunomodulatory, anti-aging, antioxidant, and anti-osteoporosis effects. Objective This study seeks to identify the optimal conditions for extracting CDPs using hot water. Additionally, it aims to evaluate their chemical properties, antioxidant activity, hypoglycemic effects, and cytotoxicity. The findings will provide a theoretical foundation for the potential use of CDPs in functional foods and pharmaceuticals. Methodology The study employed response surface methodology to optimize the hot water extraction conditions for CDPs. The extracted CDPs were characterized using a range of chemical, spectroscopic, and instrumental analyses. Furthermore, their antioxidant activity, hypoglycemic effects, and cytotoxicity were evaluated through relevant assays to assess their potential health benefits. Results Under optimal conditions, the yield of CDPs was 45.85% ± 1.91%. CDPs were identified as acidic heteropolysaccharides with a wide molecular weight distribution, ranging from 0.3 to 128.2 kDa. They were composed primarily of glucose (51.21%), arabinose (32.86%), galactose (17.88%), and smaller amounts of galacturonic acid (4.66%), rhamnose (1.85%), mannose (1.32%), glucosamine hydrochloride (1.08%), and xylose (0.56%). Antioxidant assays demonstrated that CDPs exhibited significant free radical scavenging activity, metal ion chelation, and reducing power. Additionally, CDPs inhibited α-glucosidase and α-amylase in vitro through a mixed-type mechanism, as well as static fluorescence quenching. Cytotoxicity assays showed that CDPs were nontoxic to L02 and AML12 cells. Conclusion This study offers a theoretical foundation for the potential use of CDPs in functional foods and pharmaceuticals and provides valuable insights for the development of new antioxidant and hypoglycemic agents from natural sources.
研究领域:
植物化学
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药理学
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食品科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING RESEARCH & DESIGN
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2022
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IF 4.119
Chunying Li,Fang Nie,Changyin Feng,Mengfei Tian,Meiting Yu,Chunjian Zhao,Yujie Fu
A creative dual-template molecularly imprinted polymer (Fe 3 O 4 @MCM-41-MIPs) with good magnetic properties and specific recognition of echinacoside and acteoside was prepared by surface imprinting. Echinacoside and acteoside served as dual templates, 4-vinylpyridine (4-VP) was used as a functional monomer , the cross-linker was ethylene glycol dimethacrylate (EGDMA), and the selected initiator was azobisisobutyronitrile (AIBN). The prepared Fe 3 O 4 @MCM-41-MIPs were applied to the selective separation and enrichment of echinacoside and acteoside from Cistanche deserticola Y. C. Ma ( C. deserticola ). The resulting Fe 3 O 4 @MCM-41-MIPs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), laser particle size scanning and vibration sample magnetometry (VSM). The results of the isotherm adsorption experiment showed that the maximum adsorption capacities of Fe 3 O 4 @MCM-41-MIPs for echinacoside and acteoside were 6.02 and 1.63 mg·g -1 , respectively, at 298 K. The pseudo-second-order reaction mechanism showed that the equilibrium adsorption capacities of Fe 3 O 4 @MCM-41-MIPs for echinacoside and acteoside were 4.81 and 0.93 mg·g -1 , respectively. The characterization of Fe 3 O 4 @MCM-41-MIPs showed that the imprinted layer with dual-template molecules was wrapped on the outside of the carrier, and the obtained Fe 3 O 4 @MCM-41-MIPs showed good saturation magnetization , desirable capacity for echinacoside and acteoside adsorption, and satisfactory reuse (echinacoside and acteoside maintained relative adsorption rates of 97.5% and 95.8% after five cycles). After separation, the purities of echinacoside and acteoside were 33.50% and 27.00%, respectively. In addition, in the selective adsorption experiment, the synthesized Fe 3 O 4 @MCM-41-MIPs showed better selectivity for echinacoside and acteoside than their structural analogs. Therefore, this method proved to be sustainable, rapid and highly efficient for the separation and enrichment of echinacoside and acteoside.
研究领域:
中药化学
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分离科学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2017
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IF 3.154
Yang Li,Ying Peng,Mengyue Wang,Pengfei Tu,Xiaobo Li
Cistanches Herba is taken orally as a health food supplement and medicinal plant in Asian countries. It consists of the stems of Cistanche deserticola (CD) and Cistanche tubulosa (CT). The gastrointestinal metabolism of the multiple components contained in Cistanches Herba is crucial for the discovery of bioactive constituents. This study aims to elucidate the comprehensive metabolic profile of the Cistanches Herba water extract by simulating human gastrointestinal metabolism in vitro independently and sequentially using four models: gastric juice, intestinal juice, human intestinal bacteria, and human intestinal microsomes. A total of 35 and 18 metabolites were characterized from CD and CT water extracts, respectively. These metabolites were formed through reduction, methylation, dimethylation, deglycosylation, decaffeoyl, derhamnose, dehydrogenation, and glucuronidation. The difference in metabolites of the Cistanches Herba water extract and single compounds and the difference in metabolites of CD and CT water extracts were caused by the oligosaccharides and polysaccharides in Cistanches Herba.
研究领域:
药理学
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代谢
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传统医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
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2024
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IF 6.5
Kangkang Liu,Junxiang Li,Wenting Hao,Jingjing Li,Israr Khan,Yibo Liang,Haijuan Wang,Xiaofeng Li,Chunjiang Zhang
Microbial fermentation is an effective method to enhance the bioavailability of herbs. This study utilized Lactiplantibacillus plantarum LZU-J-Q21 to ferment Cistanche deserticola and evaluated its metabolic properties and biological activity. Results showed that the contents of total acid and flavone, and the clearance rates of DPPH, ABTS and OH − in fermented Cistanche deserticola (FCD) were increased by 142.74 %, 56.45 %, 58.1 %, 62.3 %，51.2 %, compared with non-fermented Cistanche deserticola (NFCD). The metabolic profile of FCD had remarkable changes, especially elevated glucose and adenosine (97.31 % and 59.18 %). Further, FCD increased the weight-bearing swimming time of mice by 88.57 %, reduced fatigue markers BUN, BLA, and MDA (18.47 %, 12.92 %, and 15.16 %), and enhanced liver/muscle glycogen and SOD (28.99 %, 28.57 %, and 14.47 %). The investigation into its anti-fatigue mechanism suggested that FCD enhanced GS protein expression by activating PI3K/AKT/GSK3β signaling. These findings suggest that FCD enhances anti-fatigue effects by modifying its metabolic properties and biological activity.
研究领域:
微生物学
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药理学
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功能食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2024
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IF 3.8
Mujin Fang,Chen Chen,Yingying Fan,Qiong Zhang,Kui Li,Guoqing Yang,Renpan Deng,Xueqin Li
Molecularly imprinted nanocomposite membranes (MINMs) have shown great superiority in selective separation of acteoside (ACT) from phenylethanoid glycosides in Cistanche tubulosa . Herein, ACT-based MINMs (A-MINMs) with coral reef-like imprinted structure were proposed and developed for specifically separating ACT molecules. The nanospheres with hydrophilic multicores (NHMs) were introduced into polyvinylidene fluoride (PVDF) powders to obtain NHMs@PVDF membranes by a phase inversion method. Subsequently, the designed coral reef-like imprinted structure was constructed on NHMs@PVDF membrane-based surface. The A-MINMs with coral reef-like imprinted structure had dendritic and porous properties, which helped to form abundant ACT-imprinted cavities and sites of A-MINMs. In addition, the hydrophilic multicores and void space of NHMs together formed the affinity capture cages for hydrophilic ACT, enhancing rebinding capacity and permselectivity of A-MINMs. Therefore, A-MINMs with coral reef-like imprinted structure exhibited the high rebinding capacity (110.95 mg/g), rebinding selectivity of 5.15 and outstanding permselectivity of 10.04 toward ACT molecules. Moreover, the designed strategy of A-MHIMs with coral reef-like imprinted structure provides a new feasible method for permselectivity separation of other bioactive components.
研究领域:
材料科学
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生物化学
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制药工程
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产品关联
DOI
PubMed
高影响因子
Journal of Future Foods
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2025
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IF 7.2
Guoxue Zhu,Wang Wang,Yuwen Wang,Wei Li,Shilin Yi,Kai Wang,Lu Fan,Xinyu Lu,Juanjuan Tang,Ruini Chen
Parkinson's disease (PD) is a progressive neurodegenerative disorder affecting movement, with no treatments currently available to halt or slow its progression. Therefore, the development of new anti-PD drugs is urgently needed. As a kind of medicine and food homologous plant, Cistanches Herba has a promising future for the treatment of PD. In this study, a 1-methyl-4-phenyl-1,2,3,6-tetrahydropy ridine (MPTP)-induced PD mouse model was used to detect Cistanches Herba's anti-PD effects via histopathology and molecular biology. Simultaneously, the effect of Cistanches Herba on the "gut microbiota-barrier axis" was assessed through gut microbiota and intestinal barrier function in mice. Finally, transcriptomics analysis was conducted to further verify the results. As a result, 37 differential metabolites and 16 microbial genera were screened and tentatively identified. Thirty-two metabolites and sixteen microbial genera were simultaneously altered with opposing trends in variation after MPTP and Cistanches Herba treatments. We built a framework for predicting targets and hostmicrobe interaction mechanisms, as well as identifying alternative treatment for PD, which should be validated further for clinical application. In conclusion, Cistanches Herba exerts a protective effect against the development of PD by manipulating the structural feature of intestinal flora to influence the host metabolites.
研究领域:
神经病学
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分子生物学
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转化医学
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肠道微生物学
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药物代谢
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系统生物学
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营养与健康
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）