POULTRY SCIENCE
·
2022
·
IF 4.014
Mingfa Sun,Hongchao Jiao,Jingpeng Zhao,Xiaojuan Wang,Haifang Li,Yunlei Zhou,Hai Lin
Stress is a common problem diminishing the muscle development of broilers. Creatine ( Cr ), an energy buffer in skeletal muscle, plays a fundamental role in muscle physiology. This study aimed to evaluate the effect of Cr monohydrate (CMH) on protein breakdown in chicken myotubes challenged by corticosterone (CORT) in vitro. The morphology of myotube was measured and the activation of ubiquitin proteasome ( UP ) pathway was determined. The result showed that CORT treatment decreased myotube diameter ( P < 0.05), increased 3-methyl-histidine (3M-His) content in medium, enhanced the mRNA expression levels of muscle ring finger1 ( MuRF1 ) and Atrogin1 ( P < 0.001), and Atrogin1 protein level ( P < 0.05) compared with control. By contrast, CMH increased myotube diameter ( P < 0.05) and myosin heavy chain ( MHC ) expression ( P < 0.001), whereas decreased 3M-His and the mRNA and protein levels of Atrogin1 ( P < 0.05), compared to control. In the present of CMH, the decreased myotube diameter and increased 3M-His, mRNA levels of MuRF1 and Atrogin1 , and Atrogin1 protein level by CORT were partially relieved ( P < 0.05). Hence, the result suggests that CMH alleviates CORT-induced protein breakdown by suppressing Atrogin1 expression in chicken myotubes. The result highlights the potential application of CMH in regulating muscle protein catabolism in chickens under stress.
研究领域:
动物生理学
·
肌肉生物学
·
应激生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Hanghang Lou,Hongyun Lu,Shengliang Zhang,Ying Shi,Enbo Xu,Donghong Liu,Qihe Chen
Currently, various cultured meat products, including chicken, beef and pork, have been developed. However, established methods for production cultured fish meat with highly aligned myotubes are still lacking. In this study, we introduced a culture method based on high-biocompatibility fibrin hydrogels with an easy-to-use tissue mold for obtaining cultured fish fillets that closely mimicked the structure of natural fish fillets. Results showed that highly aligned myotubes were observed within the muscle bundles culturing in the tissue mold. The myotube fusion index was also increased to 72.65 %. Furthermore, key differentiation genes ( desmin , myosin light chain kinase , myocilin ) were up-regulated in the tissue mold group. Transcriptomic analysis further supported the effectiveness of method in promoting myoblast fusion. Stiffness of the muscle bundles was also positively impacted by the tissue mold. Ultimately, sensory and nutritional characteristics of natural and cultured fish fillets were compared, revealing that cultured fish fillets prepared from the tissue mold was closer to natural fish fillets in sensory characteristic, and there were still some gaps with natural fish fillets in nutritional characteristic. Overall, our findings suggest that optimizing culture methods can help bridge some gaps between natural meat and cultured meat, facilitating the development of cultured fish meat.
研究领域:
生物技术
·
组织工程
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Serge Ostrovidov,Majid Ebrahimi,Hojae Bae,Hung Kim Nguyen,Sahar Salehi,Sang Bok Kim,Akichika Kumatani,Tomokazu Matsue,Xuetao Shi,Ken Nakajima,Shizu Hidema,Makoto Osanai,Ali Khademhosseini
In this study, composite gelatin–polyaniline (PANI) nanofibers doped with camphorsulfonic acid (CSA) were fabricated by electrospinning and used as substrates to culture C2C12 myoblast cells. We observed enhanced myotube formation on composite gelatin–PANI nanofibers compared to gelatin nanofibers, concomitantly with enhanced myotube maturation. Thus, in myotubes, intracellular organization, colocalization of the dihydropyridine receptor (DHPR) and ryanodine receptor (RyR), expression of genes correlated to the excitation–contraction (E–C) coupling apparatus, calcium transients, and myotube contractibility were increased. Such composite material scaffolds combining topographical and electrically conductive cues may be useful to direct skeletal muscle cell organization and to improve cellular maturation, functionality, and tissue formation.
研究领域:
生物材料
·
组织工程
·
肌肉生理学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
·
2024
·
IF 3.8
Dong Han,Yun-Biao Chen,Kai Zhao,Hong-Zhou Li,Xing-Yu Chen,Guo-Zheng Zhu,Chen Tu,Jia-Wen Gao,Jing-Shen Zhuang,Zhi-Yong Wu,Zhao-Ming Zhong
Skeletal muscle atrophy, characterized by loss of muscle mass and function, is often linked to systemic inflammation. Tanshinone IIA (Tan IIA), a major active constituent of Salvia miltiorrhiza , has anti-inflammatory and antioxidant properties. However, the effect of Tan IIA on inflammation-induced skeletal muscle atrophy remains unclear. Here, a mice model of the inflammatory muscle atrophy was established using lipopolysaccharide (LPS). Tan IIA intervention significantly increased muscle mass and strength, improved muscle fiber size, and maintained the integrity of skeletal muscle mitochondrial morphology in LPS-treated mice. Myotubes derived from myosatellite cells (MUSCs) were exposed to LPS in vitro . Tan IIA treatment inhibited LPS-induced muscle protein degradation and increased myotube diameter. Notably, Tan IIA attenuated LPS-induced inflammatory response and hyperactive mitophagy both in vivo and in vitro. In addition, Tan IIA treatment effectively diminished oxidative stress, inhibited the accumulation of mitochondrial reactive oxygen species (mtROS), and attenuated mitochondrial fission in LPS-treated myotubes. Reducing mtROS production helped to inhibit LPS-induced excessive mitophagy and myotubes atrophy. Together, our results reveal that Tan IIA can protect against inflammation-induced skeletal muscle atrophy by regulating mitochondrial dysfunction, presenting innovative potential therapeutics for skeletal muscle atrophy.
研究领域:
药理学
·
免疫学
·
肌肉生物学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2014
·
IF 8.312
Serge Ostrovidov,Xuetao Shi,Ling Zhang,Xiaobin Liang,Sang Bok Kim,Toshinori Fujie,Murugan Ramalingam,Mingwei Chen,Ken Nakajima,Faten Al-Hazmi,Hojae Bae,Adnan Memic,Ali Khademhosseini
Engineering functional muscle tissue requires the formation of densely packed, aligned, and mature myotubes. To enhance the formation of aligned myotubes with improved contractibility, we fabricated aligned electrospun gelatin multi-walled carbon nanotubes (MWNTs) hybrid fibers that were used as scaffolds for the growth of myoblasts (C2C12). The MWNTs significantly enhanced myotube formation by improving the mechanical properties of the resulting fibers and upregulated the activation of mechanotransduction related genes. In addition, the fibers enhanced the maturation of the myotubes and the amplitude of the myotube contractions under electrical stimulation (ES). Such hybrid material scaffolds may be useful to direct skeletal muscle cellular organization, improve cellular functionality and tissue formation.
研究领域:
组织工程
·
生物材料
·
细胞生物学
·
肌肉骨骼研究
✓ 已验证
DOI
PubMed
高影响因子
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
·
2022
·Vol. 322
·期 5
·C1022
·
IF 5.282
Mingfa Sun,Hongchao Jiao,Xiaojuan Wang,Haifang Li,Yunlei Zhou,Jingpeng Zhao,Hai Lin
Creatine (Cr) is beneficial for increasing muscle mass and preventing muscle atrophy via involving in energy metabolism through the Cr and phosphocreatine (PCr) system. This study aimed to evaluate the supplemental effect of Cr on protein metabolism under normal and starvation conditions. The primary myoblasts were obtained from the breast muscle of chicks. The mammalian target of rapamycin (mTOR)/P70S6 kinase (P70S6K), ubiquitin-proteasome (UP) pathways, and mitochondrial function of myotubes were evaluated at normal or starvation state and with or without glucose supplementation. Under normal condition, Cr supplementation enhanced protein synthesis rate as well as upregulated the total and phosphorylated P70S6K expressions. Cr had little influence on protein catabolism and mitochondrial function. In a starvation state, however, Cr alleviated myotube atrophy and enhanced protein accretion by inhibiting Atrogin1 and myostatin (MSTN) expression. Furthermore, Cr treatment upregulated the transcriptional coactivators peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) expression and decreased reactive oxygen species (ROS) accumulation under starvation condition. In the presence of glucose, however, the favorable effect of Cr on protein content and myotube diameter did not occur under starvation condition. The present result indicates that at a normal state, Cr stimulated protein synthesis via the mTOR/P70S6K pathway. In a starvation state, Cr mainly takes a favorable effect on protein accumulation via suppression of the UP pathway and mediated mitochondrial function mainly by serving as an energy supplier. The result highlights the potential clinical application for the modulation of muscle mass under different nutritional conditions.
研究领域:
肌肉生物学
·
营养学
·
细胞代谢
·
蛋白质合成
·
能量代谢
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Yawen Chen,Wenjing Dai,Wanli Xiong,Yi Zhou,Ziying Chen,Chengxin Ge,Yan Zhou
As a meat substitute, cell cultured meat aims to simulate the composition of real meat to provide nutrient substance. The myotube formation efficiency of myoblasts in vitro is the key for simulating the structure and composition of muscle tissue. Therefore, this study is aimed to explore the most effective groove widths for myoblasts fusion and related molecular mechanism. The results of immunofluorescence showed that the grooves with a width range of 50 μm–1000 μm were beneficial to cell arrangement compared to flat structure. At the same time, the expression of myogenin ( Myog ) and myosin heavy chain 2 ( Myh2 ) up-regulated. However, the cell fusion index of myoblasts was higher in grooves with a width of 100 μm, 200 μm and 300 μm. Furthermore, Cytochalasin D disrupted intracellular stress fibers and myotube formation. However, there were still many myotubes formed in the 200 μm groove with the addition of Cytochalasin D. The expression of fusion related genes was higher than the flat group. Moreover, incorporating groove structure into scaffolds of soybean protein isolate and pea protein isolate could enhance cell adhesion, activity and myogenic differentiation of myoblasts. This could provide ideas for scaffolds design of cultured meat products using groove structure.
研究领域:
组织工程
·
细胞生物学
·
生物技术
·
食品科学
✓ 已验证
DOI
高影响因子
REJUVENATION RESEARCH
·
2020
·Vol. 23
·期 5
·367-376
·
IF 0
Mei Wang,Shan-Quan Chang,Yu-Shan Tian,Ge-Qiang Zhang,Jin Qi
The incidence of type 2 diabetes mellitus (T2DM) has been increasing in recent years and has become a serious threat to human health. Zengye Decoction (ZYD), a well-known traditional Chinese medicinal formula, has been used in the treatment of T2DM with yin asthenia and extreme heat since Qing Dynasty. However, the characteristics of antidiabetic activities of ZYD have not been fully elucidated. In our study, high-fat diet and streptozotocin were used to establish the T2DM rat model. After 3 weeks of treatment with ZYD, the fasting blood glucose (FBG), oral glucose tolerance, the fasting serum insulin concentration, insulin sensitivity index (ISI), serum lipid profiles, and pancreas histopathology were measured. Then, under circumstance of insulin-resistant glucose consumption, 2-(N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) uptake and glycogen content in C2C12 myotubes, 3T3-L1 adipocytes, and HepG2 cells were determined, respectively. Finally, the expressions of key targets in the insulin signaling pathway were measured to explain the potential mechanism underlying these activities. After administration with ZYD, a notable reduction in FBG levels, oral glucose tolerance test-area under the curve, blood lipid metabolism, and ISI values were observed compared with the diabetic control group. Moreover, ZYD restored the damaged islet cells in T2DM rats. Significant increases in glucose consumption, glucose uptake, glycogen content, expression of glucose transporter type 4, and the ratio of p-Akt/Akt were observed in the ZYD groups. According to the above results, ZYD exhibited glucose disposal, including glucose consumption, glucose uptake, and glycogen content and promoted the Akt signal pathway, which indicates that ZYD exerts significant hypoglycemic effect in T2DM.
研究领域:
药理学
·
内分泌学
·
中医
✓ 已验证
产品关联
DOI
PubMed
TOXICOLOGY IN VITRO
·
2021
·
IF 3.5
Shuyu Ouyang,Dachuan Zhang,Tengfei Zhu,Shuting Yu,Wunier,Qian-Nan Hu,Yingying Le
β 2 -adrenoceptor (β 2 AR) agonists can stimulate skeletal muscle growth. Their illegal use in food-producing animals, human athletes and bodybuilders causes adverse health effects. In the present study, we developed 3D-QSAR models for predicting the activity of chemicals which can stimulate skeletal muscle growth through β 2 AR. The activity of 25 β 2 AR agonist s was measured by β 2 AR-cAMP response element (CRE) –luciferase (Luc) reporter assay. The 3D-QSAR models were built using comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA). The CoMFA and CoMSIA models displayed high external predictability (R 2 0.996 and 0.992, respectively) and good statistical robustness, and revealed that electrostatic effects were the most prominent forces influencing the activity of β 2 AR agonists. The CoMFA and CoMSIA contour plots provided clues regarding the main chemical features responsible for the activity variations and also resulted in predictions which correlate very well with the observed activity. In vitro study with differentiated myotubes showed that the potency orders of β 2 AR agonists in activating the β 2 AR-CRE-Luc reporter and in upregulating CREB target genes related to muscle growth were consistent. These 3D-QSAR models provide tools for predicting the activity of chemicals which might be illegally used in livestock or humans to stimulate skeletal muscle growth.
研究领域:
药理学
·
分子生物学
·
生物信息学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Longkang Li,Huipeng Li,Huan Zhou,Zhentian Diao,Yanjie Bai,Lei Yang
The repair capacity of skeletal muscle is severely diminished in massive skeletal muscle injuries accompanied by inflammation, resulting in muscle function loss and scar tissue formation. In current work, we developed a tannic acid and silicate ions functionalized tissue adhesive poly (vinyl alcohol) (PVA)-starch composite hydrogel, referred as PSTS. It was formed based on the hydrogen bonds of tannic acid to organic polymers, as well as silicate-tannic acid ligand interaction. PSTS could be gelatinized in minutes at room temperature with crosslinked networks formation, making it applicable for injection. Further investigations revealed that PSTS owned skeletal muscle comparable conductivity and modulus to act as a temporary platform for muscle repairing. Moreover, PSTS could release tannic acid and silicate ions in situ to inhibit bacterial growth, induce vascularization, and reduce antioxidation, paving the possibility to create a favorable microenvironment for skeletal muscle regeneration and tissue fibrosis control. The in vivo model confirmed that PSTS could enhance muscle fibers regeneration and myotubes formation, as well as reduce infection and inflammation risk. These findings thereby implied the great potential of PSTS in the treatment of formidable skeletal muscle injuries.
研究领域:
生物材料
·
组织工程
·
再生医学
·
生物医学工程
·
肌肉骨骼研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2019
·Vol. 107
·期 6
·1264-1272
·
IF 3.221
Tao Huang,Jiangwei Xiao,Shuhao Wang,Zhaohong Liao,Tao Huang,Ruicai Gu,Junhua Li,Gang Wu,Hua Liao
Introducing or grafting molecules onto biomaterial surfaces to regulate muscle cell destination via biophysical cues is one of the important steps for biomaterial design in muscle tissue engineering. Therefore, it is important to understand the interaction between myoblasts and myofibers with substrates modified by biomimetic layer with different thicknesses. In this study, we used a surface-induced atom transfer radical polymerization method to synthetize and graft poly-phenoxyethyl methacrylate (PHEMA) brushes having different lengths on the glass substrates. C2C12 myoblasts were seeded on the PHEMA brushes and differentiated using horse serum, for analyzing the sensibility of muscle cells to feel environment changing, and further investigating whether the depths of grafting layer on the biomaterial surface are important factors in regulating muscle cell behaviors. Our results demonstrated that on the thicker PHEMA brushes surface (200 and 450 nm), C2C12 myoblasts showed a better survival and proliferation and were favorable for cell fusion and myotube formation. Furthermore, myofibers survived on the thicker brushes were more functional and upregulated cytoskeleton proteins (tubulin, vimentin, and vinculin) and FAK levels, and enhanced the expression levels for mechanical stress molecules (HGF, NOS-1, and c-Met). These results suggest that grafting thickness of PHEMA layer on the substrate led to the myoblasts/myofiber behavior change, which would be valuable for the design and preparation of the modified layer on muscle tissue engineering scaffolds. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1264–1272, 2019.
研究领域:
生物材料
·
组织工程
·
细胞生物学
·
肌肉生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Wenbo Niu,Zhenjun Chen,Fujing Wang,Chi Zhang,Hao Wang,Jinyue He,Zhuoen He,Jian Zhang,Haitao Yuan,Mingqing Wang
Chemotherapeutic drugs used for patients with malignant diseases often lead to skeletal muscle atrophy, which is a devastating side effect and contributes to decreased quality of life and survival. Overproduction of reactive oxygen species (ROS) and chronic unresolved inflammation have been shown as major drivers of chemotherapeutic agents-induced skeletal muscle atrophy. Nanomedicine that can scavenge ROS and relieve inflammation have the potential to treat chemotherapy-associated muscle wasting. Here we prepared a simple and efficient one-step development of engineered manganese-based nanozymes (Mn-PDAs) and demonstrated their potential as therapeutic agents for chemotherapy-induced muscle atrophy. Mn-PDAs with multiple enzyme-mimicking activities could effectively scavenge abroad spectrum of ROS and repress proinflammatory cytokines production in vitro . Mn-PDAs could also efficiently suppress ROS-induced myotube apoptosis, promote mitochondrial biogenesis, ameliorate mitochondrial dysfunction and exert a robust anti-inflammatory effect by inhibiting the infiltration of pro-inflammatory M1 macrophages in the local muscle environment, therefore alleviating cisplatin-associated muscle atrophy in mice. Mechanistically, transcriptomic analysis revealed that PI3K/AKT/mTOR signal pathway activation involved in Mn-PDAs-prevented muscle atrophy. Our study presents a therapeutic strategy against chemotherapy-induced muscle atrophy, and also highlights the potential of Mn-PDAsbased therapeutics to treat other oxidative stress-associated diseases.
研究领域:
纳米技术
·
药理学
·
肌肉骨骼系统
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）