Water
·
2022
·Vol. 14
·期 8
·1223
·
IF 3.53
Xiaoqun Zhang,Weiyou Zheng,Heng Zhang,Yi Chai,Guoliang Ruan
In order to understand the difference in muscle nutritional quality between yellow catfish (Pelteobagrus fulvidraco) cultured in in-pond raceway systems (IPR) and traditional ponds (TRP), two modes were used to culture the yellow catfish with the same initial body weight [average body weight (15.69 1 ± 2.28) g] for 60 days. The growth index, muscle texture characteristics, muscle nutritional components, amino acids and fatty acids of the fish were measured after the culture experiment. The results showed that the weight gain rate, specific growth rate and survival rate of IPR were significantly higher than TRP (p< 0.05). The hardness, elasticity, chewiness and resilience of the yellow catfish cultured in IPR were significantly higher than those cultured in TRP (p< 0.05). The crude protein content in the muscle of the yellow catfish cultured in IPR was significantly higher than that cultured in TRP (p< 0.01), while the content of crude fat and water was significantly lower (p< 0.01). The total amount of amino acids, essential amino acids and flavor amino acids of IPR were significantly higher than TRP (p< 0.01). The percentages of saturated fatty acids in muscle of the yellow catfish cultured in IPR and TRP were 3.59% and 3.83%, respectively, and the percentages of unsaturated fatty acids were 96.41% and 96.17%, respectively. It was concluded that the nutritional quality of yellow catfish cultured in IPR was better than that of fish cultured in TRP.Keywords:pond;catfish;water quality;growth;muscular texture;nutritional component
研究领域:
水产养殖学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Feng Wang,Soottawat Benjakul,Zhiyu Liu,Lukai Ma,Bin Zhang
Shrimp intestine has great effect on the muscle quality during long term storage. This study examined the effects of shrimp intestine on the muscle quality of red shrimp ( Solenocera crassicornis ) during cold storage. Removing the intestine significantly reduced TVC, TVB-N, and TCA-induced protein precipitation levels, mitigating muscle quality deterioration. Proteomic analysis revealed that intestinal proteins (7.6–621.4 kDa) and muscle proteins (1.8–1328.3 kDa) influenced muscle protein metabolism via structural proteins and metabolic enzymes. Peptidomic analysis identified 519 differential peptides (261 upregulated, 258 downregulated) in TS6 vs. CS6 after six days of storage, linked to proteolysis and microbial infection pathways. Additionally, Vibrio and Aliivibrio genera in the intestine increased significantly during storage, with Vibrio abundance accelerating muscle protein degradation. These findings provide insights into improving cold storage techniques and spoilage prevention for aquatic products.
研究领域:
食品科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·Vol. 59
·期 4
·2249-2261
·
IF 2.6
Jianyou Zhang,Aodong Yue,Lei Sun,Lifeng Fei,Pengbo Cui,Yue Qiu,Fei Lyu,Yuting Ding
Summary This study aimed to evaluate the effect of chitosan (CHI) combined electrolytic water (EW) cold sterilisation on muscle quality and myofibril proteins (MP) of large yellow croaker ( Pseudosciaena crocea ) during 12 days of refrigerated storage (4 °C). After the storage period, the sample of the EW + CHI group had the highest rate of hardness (36.68%), springiness (16.11%) among the control group without any treatment (CON), CHI group and EW group and similar tendencies were found in chewiness and cohesiveness. The MP content in the CON group decreased sharply from 80.35 mg g −1 to 57.90 mg g −1 , in contrast to the EW + CHI group, which decreased from 81.65 mg g −1 initially to 80.83 mg g −1 . The conformation of MP α-helix and β-sheet was retained to protect the secondary structure, and the exposure of aromatic amino acid residues was constrained to defend the tertiary structure in EW + CHI treatment which effectively delayed the degradation of MP. Based on these outcomes, EW + CHI treatment could significantly maintain the MP of large yellow croakers and prevent corruption during refrigerated storage.
研究领域:
食品科学
·
蛋白质化学
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE INTERNATIONAL
·
2025
·Vol. 33
·期 1
·1-18
·
IF 2.4
Sha Yuanpu,Chen Yuhuan,Dong Jing,Gao Xiaofei,Yuan Huatao,Zhang Jingxiao,Gao Yunni,Li Xuejun
This study aims to verify the effects of astaxanthin microcapsules on the growth, physiology, and nutritional value of Cyprinus carpio ( C. carpio ), and evaluate the feasibility and safety of microcapsule technology in aquaculture. In this experiment, natural and synthetic astaxanthin were encapsulated in microcapsules, with empty microcapsules used as a control, and C. carpio was fed these diets for 8 weeks. Growth, muscle quality, and the antioxidant system were then assessed. The results showed that (1) the weight gain rate (WGR), specific growth rate (SGR), and condition factor (K) in group A (natural astaxanthin) and group B (synthetic astaxanthin) were significantly higher than those in the control group, with no significant differences in viscerosomatic index (VSI) or hepatosomatic index (HSI) across the groups. (2) Astaxanthin supplementation enhanced the antioxidant activity in the hepatopancreas of C. carpio . Total superoxide dismutase (T-SOD) activity significantly increased, malondialdehyde (MDA) levels decreased, while no significant changes were observed in total antioxidant capacity (T-AOC) or catalase (CAT) activity. The sod1 and gpx1a genes were significantly upregulated in group A. (3) Astaxanthin also improved muscle nutritional quality by significantly increasing crude protein content, reducing crude ash content, and enhancing water-holding capacity. Amino acid and fatty acid composition also showed improvement. Overall, this study suggests that natural astaxanthin may be superior to synthetic astaxanthin, and provides preliminary evidence that self-made astaxanthin microcapsules are safe for future aquaculture, offering a scientific basis for sustainable and eco-friendly aquaculture practices.
研究领域:
水产养殖学
·
营养学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 23
·3928
·
IF 5.561
Yuexiang Zhan,Chuanhai Tu,Huili Jiang,Soottawat Benjakul,Jilong Ni,Kaixuan Dong,Bin Zhang
This study explored the effects of sous vide (SV) cooking treatments on the physicochemical quality and volatile flavor of half-shell scallop (Chlamys farreri) during 30 d of chilled storage. The vacuum-packed scallop samples were cooked at 70 °C (SV-70) and 75 °C (SV-75) and maintained for 30 min. The samples were compared with the positive control (cooked at 100 °C for 10 min, CK). The results indicate that the total volatile basic nitrogen (TVBN), pH, texture, and malondialdehyde (MDA) content gradually increased, while the myofibrillar protein (MP) extraction rate of the CK, SV-70, and SV-75 samples significantly decreased with increasing chilled storage time. Significantly, the SV cooking treatments maintained a much higher water-holding capacity of scallop muscle, compared with the conventional cooking process at 100 °C. Additionally, the SV-75 cooking treatment maintained relatively stable TVBN, pH, and MDA content, springiness, and shearing force properties of scallop samples, especially during 0–20 d of storage. Volatile flavor analysis showed that a total of 42 volatile organic compounds (VOCs) were detected in the scallop samples, and there were no considerable differences in these VOCs between the CK and SV-75 cooked samples (0 d). Overall, the SV cooking treatments effectively maintained acceptable and stable physicochemical and volatile flavor properties of half-shell scallop samples during chilled storage.Keywords:sous vide;scallop;muscle quality;volatile compounds;chilled storage
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2024
·
IF 3
Hao Liu,Ruitao Xie,Weibin Huang,Yuanzhi Yang,Menglong Zhou,Baiquan Lu,Biao Li,Beiping Tan,Xiaohui Dong
This study investigated the effects of varying doses of dietary aflatoxin B1 (AFB1) on the growth, intestinal health, and muscle quality of hybrid grouper. Four diets with varying AFB1 concentrations (0, 30, 445, and 2,230 μg kg−1) were used. Elevating AFB1 concentrations led to a decline in growth indexes, specifically the weight gain rate and the specific growth rate, although the survival rate remained unchanged. Morphological indicators showed a dose-dependent decline with AFB1 exposure. Intestinal MDA content and hindgut reactive oxygen species (ROS) levels increased, while antioxidant indexes and digestive enzymes decreased with higher AFB1 levels. AFB1 negatively influenced hindgut tight junction protein and antioxidant-related gene expression while promoting inflammation-related gene expression. The presence of AFB1 in the experiment led to a decrease in beneficial intestinal bacteria, such as Prevotella, and an increase in harmful intestinal bacteria, such as Prevotellaceae_NK3B31_group. Muscle lipid and unsaturated fatty acid content significantly decreased, while muscle protein and liver AFB1 content increased dramatically with higher AFB1 concentrations. AFB1 caused myofibrillar cleavage and myofilament damage, leading to increased spaces between muscle fibers. In conclusion, diets with AFB1 levels exceeding 30 μg kg−1 inhibited hybrid grouper growth, while levels surpassing 445 μg kg−1 resulted in hindgut ROS accumulation, inflammation, elevated intestinal permeability, reduced digestive enzyme activity, and compromised muscle quality.
研究领域:
水产养殖学
·
毒理学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2024
·Vol. 2024
·期 1
·5205506
·
IF 3
Dan Liu,Jiali Mi,Xiao Yan,Chaobin Qin,Junli Wang,Guoxing Nie
In the present study, we conducted a 10-week culture experiment to investigate the effects of taurine on the growth performance, antioxidant properties, and muscle quality of the common carp fed an oxidized lipid diet. There were five experimental groups with three replicates each. Based on the fresh fish oil group (FO), equal amounts of oxidized fish oil (with a thiobarbituric acid-reactive substances value of 49.57 ± 2.34 mg/kg) and 0 g/kg (OFO), 4 g/kg (OT4), 8 g/kg (OT8), and 12 g/kg (OT12) taurine were added to the diet, while the same composition ratio was maintained by changing the microcrystalline cellulose content. Compared to the FO group, the feed conversion ratio, weight gain rate, muscle crude lipid, and n-3/n-6 polyunsaturated fatty acids (PUFA) ratio were significantly lower in the group OFO ( P < 0.05). In addition, compared to the FO group, OFO fish showed an increased content of malondialdehyde and protein carbonylation and decreased hardness, brightness, pH, superoxide dismutase, and catalase levels in the muscle ( P < 0.05). Notably, the growth index significantly improved in the OT4 group compared to that in the OFO group ( P < 0.05). In addition, dietary taurine increased the crude lipid content, n-3/n-6 PUFA, antioxidant capacity, hardness, springiness, brightness, pH, and collagen content in the muscle compared with OFO fish ( P < 0.05). Moreover, taurine supplementation significantly reduced myofiber diameter and increased myofiber density ( P < 0.05) and enhanced the expression levels of paired box 7 ( pax7 ), myogenic factor 5 ( myf5 ), myogenic differentiation antigen ( myod ), and myogenic regulatory factor 4 ( mrf4 ) compared with that of the OFO group ( P < 0.05). Considering growth performance and muscle quality, the optimal supplemental levels of taurine in the oxidized lipid diet were 4 g/kg and 6.84–7.70 g/kg, respectively.
研究领域:
水产养殖
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Ahtisham Ali,Jinfang Wang,Wei Jiang,Shuai Wei,Qinxiu Sun,Qiuyu Xia,Zefu Wang,Zongyuan Han,Yang Liu,Shucheng Liu
Differential metabolites and potential biomarkers of hybrid grouper and golden pompano muscles were identified in this study. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to evaluate liquid chromatography-mass spectrometry (LC-MS) metabolomic analysis. Among the metabolites, 1678 co-contained differential metabolites in positive modes and 1445 co-contained differential metabolites in negative modes were investigated. Sixteen most differential metabolites were determined as potential biomarkers in different muscle groups. The top 10 KEGG-enriched pathways were identified. The sensory characteristics of groups were highest in aroma and sulfur-organic compounds in both fishes. Most organic acid contents in hybrid grouper were significantly higher than those of golden pompano. This present study provides a baseline for a better understanding of potential metabolites involved in muscle quality and taste as well as could provide a piece of further knowledge to identify the significant role of key biomarkers in fish muscles.
研究领域:
水产科学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 21
·3472
·
IF 4.7
Jia Li,Yuxin Liu,Huanhuan Yang,Luyun Cai,Wenqian Nong,Weiliang Guan
Water-free transportation (WFT) causes shrimp (Penaeus vannamei) flesh quality deterioration. However, the roles of endogenous protease-induced protein hydrolysis have been neglected in the research. In the present study, calpain zymography, gelatinase zymography, the hematoxylin–eosin staining method, and other methods were applied to investigate the response of various endogenous proteases (cathepsin, calpain, and gelatinase), the myofibril fragmentation index (MFI), and the microscopic morphology of shrimp muscle during WFT in comparison with the shrimp under the conventional water transportation strategy (WT). The results showed that the total activity of proteases in shrimp muscle increased significantly (p≤ 0.05) after simulated transportation. Cathepsins and gelatinases were activated during WFT. No significant (p> 0.05) changes of the activity of caspase-3 and the muscle cell apoptosis rate were detected in shrimp muscle cells after WFT. In addition, the MFI increased and the gap among muscle fiber bundles enlarged after WFT. Compared with WFT, no significant (p> 0.05) effect on the activities of calpain, gelatinase, and caspase-3 in the muscle of shrimp was found after WT, and only the activity of cathepsin L significantly increased (p≤ 0.05). Based on the findings, we concluded that the activation of various endogenous proteases was induced during WFT.
研究领域:
水产养殖
·
蛋白质组学
·
食品科学
·
动物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2020
·Vol. 12
·期 1
·144-153
·
IF 4.171
Shuge Liu,Yunmei Sun,Rui Zhao,Yingqian Wang,Wanrong Zhang,Weijun Pang
Isoleucine (Ile), as a branched-chain amino acid (BCAA), has a vital role in regulating body weight and muscle protein synthesis. However, the regulatory effect of Ile on muscle mass under high-fat diet (HFD) conditions and intramyocellular lipid deposition remains largely unclear. In this study, a feeding experiment with HFD with or without 25 g L−1 Ile was performed using 32 wild male C57BL/6J mice randomly divided into two groups. The results showed that Ile significantly increased both muscle and fat mass, as well as causing insulin resistance and meanwhile upregulating the levels of key adipogenic and myogenic proteins. More importantly, Ile damaged the mitochondrial function by vacuolation, swelling and cristae fracture in the gastrocnemius (GAS) and tibialis anterior (TA) with downregulation of mitochondrial function-related genes. Furthermore, Ile promoted myogenesis and more lipid droplet accumulation in myotubes. Compared with the control, the protein levels of myosin heavy chain (MyHC), myoblast determination protein 1 (MyoD), myogenin (MyoG), peroxisome proliferator-activated receptor gamma (PPARg) and fatty acid synthase (FAS) were upregulated in the Ile group, whereas the protein levels of adipose triglyceride lipase (ATGL) and lipoprotein lipase (LPL) were downregulated. Collectively, Ile increased muscle mass through myogenesis and intramyocellular lipid deposition. Our findings provide a new perspective for not only improving the lean juiciness of farm animals by increasing intramyocellular lipid accumulation, but also modulating myopathies under obesity.
研究领域:
营养学
·
代谢学
·
分子生物学
·
生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cachexia Sarcopenia and Muscle
·
2022
·Vol. 13
·期 6
·2740-2751
·
IF 12.063
Fengju Li,Ting Luo,Honghui Rong,Lu Lu,Ling Zhang,Chuanfeng Zheng,Dali Yi,Yi Peng,Enyu Lei,Xiaotao Xiong,Fengchao Wang,Jose M. Garcia,Ji-an Chen
Background Di-(2-ethylhexyl) phthalate (DEHP) and its metabolites can cross the placenta and may cause birth defects and developmental disorders. However, whether maternal DEHP exposure affects skeletal muscle development in the offspring and the pathways involved are unknown. This study investigated the effects of maternal DEHP exposure and the contribution of myostatin (MSTN) to skeletal muscle development in the offspring. Methods Pregnant wild-type and muscle-specific myostatin knockout (MSTN KO) C57BL/6 mice were randomized to receive vehicle (corn oil) or 250 mg/kg DEHP by gavage every other day until their pups were weaned (postnatal day 21 [PND21]). Body weights of the offspring mice were measured longitudinally, and their hindleg muscles were harvested at PD21. Also, C2C12 cells were treated with mono-2-ethylhexyl phthalate (MEHP), the primary metabolite of DEHP, and proteolysis, protein synthesis, and myogenesis markers were measured. The contribution of myostatin to maternal DEHP exposure-induced muscle wasting in the offspring was determined. Results Maternal DEHP exposure reduced body weight growth, myofibre size, and muscle mass in the offspring compared to controls (Quad: 2.70 ± 0.1 vs. 3.38 ± 0.23, Gastroc: 2.29 ± 0.09 vs. 2.81 ± 0.14, Tibialis: 1.01 ± 0.07 vs. 1.25 ± 0.11, mg/tibial length in mm, all P < 0.01, n = 35). Maternal DEHP exposure significantly increased Myostatin expression (2.45 ± 0.41 vs. 0.03 ± 0.00 DEHP vs. controls, P < 0.01, n = 5), Atrogin-1(2.68 ± 0.65 vs. 0.63 ± 0.01, P < 0.05, n = 5), MuRF1 (1.56 ± 0.51 vs. 0.31 ± 0.01, P < 0.05, n = 5), and Smad2/3 phosphorylation (4.12 ± 0.35 vs. 0.49 ± 0.18, P < 0.05), and decreased MyoD (0.27 ± 0.01 vs. 1.52 ± 0.01, P < 0.05, n = 5), Myogenin (0.25 ± 0.03 vs. 1.95 ± 0.56, P < 0.05, n = 5), and AKT phosphorylation (4.12 ± 0.35 vs. 1.00 ± 0.06, P < 0.05, n = 5), in skeletal muscle of the offspring in MSTN flox/flox , but not in MSTN KO mice. Maternal DEHP exposure resulted in up-regulation of CCAAT/enhancer-binding protein δ (C/EBPδ, 4.12 ± 0.35 vs. 1.00 ± 0.19, P < 0.05, n = 5) in skeletal muscle of the offspring in MSTN flox/flox and MSTN KO mice (4.12 ± 0.35 vs. 4.35 ± 0.28, P > 0.05, n = 5). In vitro, C/EBPδ silencing abrogated the MEHP-induced increases in Myostatin, MuRF-1, and Atrogin-1 and decreases in MyoD and Myogenin expression. Conclusions Maternal DEHP exposure impairs skeletal muscle development in the offspring by enhancing the C/EBPδ-myostatin pathway in mice.
研究领域:
发育生物学
·
毒理学
·
肌肉生理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2025
·Vol. 2025
·期 1
·5623889
·
IF 3.9
Xianglin Cao,Rongjie Yuan,Yi Guo,Mengtao Jia,Yinyin Wei,Jiameng zhou,Han Cui,Baohua Li,Jianjun Chen
L-Carnitine is widely recognized for its involvement in lipid metabolism, but its effects on muscle quality and gut health in carp have not been well studied. The research aimed to investigate how L-carnitine influences muscle quality and intestinal health in high-fat-fed carp. The study was separated into four groups that received either the standard diet, a high-fat diet (HFD), or a HFD supplemented with 500 mg/kg L-carnitine (LLC), or a HFD supplemented with 1000 mg/kg L-carnitine (HLC) for 56 days. L-Carnitine was found to significantly reduce blood lipid levels. In addition, L-carnitine increased the crude protein content and decreased the crude fat content of high-fat-fed carp muscle while improving muscle fiber morphology and muscle quality. L-Carnitine increased the expression of genes related to intestinal tight junction proteins ( claudin-2 , occludin , and zo-1 ), improved the expression of genes related to intestinal inflammation, and enhanced the physical barrier function and organization of the intestine. Analysis of intestinal flora and intestinal metabolites showed that L-carnitine increased the diversity of the intestinal flora, increased the abundance of Cetobacterium , and influenced intestinal levels of bile acids, arachidonic acid, and tryptophan-related metabolites. In conclusion, supplementation with 1000 mg/kg L-carnitine improved muscle quality and intestinal health significantly in high-fat-fed carp by regulating muscle nutrient metabolism and intestinal flora.
研究领域:
水产养殖
·
营养学
·
生理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）