National Science Review
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2024
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IF 16.3
Qiu Peng-Fei,Qiang Lei,Kong Weiqing,Wang Fang-Zhou,Wang Hong-Qin,Hou Ke-Xin,Liu Yihao,Li Cheng-Hui,Zheng Pengfei
The escalating prevalence of skeletal muscle disorders highlights the critical need for innovative treatments for severe injuries like volumetric muscle loss. Traditional treatments, such as autologous transplants, are constrained by limited availability and current scaffolds often fail to meet complex clinical needs. This study introduces a new approach to VML treatment using a shape memory polymer (SMP) based on block copolymers of perfluoropolyether and polycaprolactone diol. This SMP mimics the biomechanical properties of natural muscle, exhibiting a low elastic modulus (2–6 MPa), high tensile strength (72.67 ± 3.19 MPa), exceptional toughness (742.02 ± 23.98 MJ m−3), and superior biocompatibility, thereby enhancing skeletal muscle tissue integration and regeneration within 4 weeks. Moreover, the polymer's shape memory behavior and ability to lift over 5000 times its weight showcase significant potential in both severe muscle disorder treatment and prosthetic applications, surpassing existing scaffold technologies. This advancement marks a pivotal step in the development of artificial muscles for clinical use.
研究领域:
生物医学工程
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材料科学
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组织工程
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DOI
PubMed
高影响因子
Molecular Therapy-Nucleic Acids
·
2023
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IF 8.8
Xinyi Gu,Shen Wang,Dongdong Li,Bo Jin,Zhidan Qi,Jin Deng,Chen Huang,Xiaofeng Yin
Peripheral nerve injury can lead to progressive muscle atrophy and poor motor function recovery, which is a difficult point of treatment, and the mechanism needs to be further explored. In previous studies we found that miR-142a-3p was significantly upregulated and persistently high expressed in denervated mouse skeletal muscle. Here, we show that overexpression of miR-142a-3p inhibited the growth and differentiation of C2C12 myoblast, while knockdown of miR-142a-3p had a promoting effect. In vitro, knockdown of miR-142a-3p in denervated mouse skeletal muscle effectively increased proliferating muscle satellite cells and ameliorated muscle atrophy. Mechanistically, the myoregulator Mef2a was proved to be an important downstream target of miR-142a-3p, and miR-142a-3p regulates skeletal muscle differentiation and regeneration by inhibiting the expression of Mef2a. The co-knockdown of Mef2a and miR-142a-3p effectively alleviated or offset the biological effects of miR-142a-3p knockdown. In conclusion, our data revealed that miR-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a.
研究领域:
神经科学
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分子生物学
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肌肉骨骼疾病
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2017
·Vol. 24
·期 10
·9379-9386
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IF 2.741
Jia Yuyu,Wang Lin,Qu Zhipeng,Wang Chaoyi,Yang Zhaoguang
Three fish species ( Carassius auratus , Pelteobagrus fulvidraco , and Squaliobarbus curriculus ) were collected from Xiang River near Changsha City, Southern China. The concentrations of heavy metals including arsenic (As), cadmium (Cd), copper (Cu), iron (Fe), manganese (Mn), lead (Pb), and zinc (Zn) in the muscle, gill, and liver of three species were determined by the inductively coupled plasma mass spectrometry method. One-way analysis of variance (ANOVA) was introduced to determine the significant variations ( p < 0.05) of heavy metals. Livers were found to accumulate Cd and Cu due to the metallothionein proteins. High levels of Mn and Pb in the gills indicated that the main uptake pathway of these heavy metals was from the water. The carnivorous species, P. fulvidraco , was found to accumulate the highest levels of toxic elements (As, Cd, and Pb), while relatively high concentrations of nutrient elements (Cu, Fe, Mn, and Zn) were accumulated in omnivorous species ( C. auratus and S. curriculus ). According to the results of Pearson’s correlation analysis, there were few significant relationships at p < 0.01 level between the concentrations of the analyzed elements and the fish sizes. The results of risk assessment indicated that exposure to the toxic heavy metals from fish muscle consumption posed no non-carcinogenic health risk to local inhabitants.
研究领域:
生态毒理学
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环境科学
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DOI
PubMed
高影响因子
Cell Communication and Signaling
·
2023
·Vol. 21
·期 1
·1-18
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IF 8.4
Liao Zhaohong,Lan Haiqiang,Jian Xiaoting,Huang Jingwen,Wang Han,Hu Jijie,Liao Hua
Background To explore the role of skeletal muscle specific TGF-β signaling on macrophages efferocytosis in inflamed muscle caused by Cardiotoxin (CTX) injection. Methods CTX myoinjury was manipulated in TGF-βr2 flox/flox (control) mice or transgenic mice with TGF-β receptor 2 (TGF-βr2) being specifically deleted in skeletal muscle (SM TGF-βr2 −/− ). Gene levels of TGF-β signal molecules, special inflammatory mediators in damaged muscle or in cultured and differentiated myogenic precursor cells (MPC-myotubes) were monitored by transcriptome microarray or qRT-PCR. TGF-β pathway molecules, myokines and embryonic myosin heavy chain in regenerating myofibers, the phenotype and efferocytosis of macrophages were evaluated by immunofluorescence, immunoblotting, Luminex, or FACS analysis. In vitro apoptotic cells were prepared by UV-irradiation. Results In control mice, TGF-β-Smad2/3 signaling were significantly up-regulated in regenerating centronuclear myofibers after CTX-myoinjury. More severe muscle inflammation was caused by the deficiency of muscle TGF-β signaling, with the increased number of M1, but the decreased number of M2 macrophages. Notably, the deficiency of TGF-β signaling in myofibers dramatically affected on the ability of macrophages to conduct efferocytosis, marked by the decreased number of Annexin-V − F4/80 + Tunel + macrophages in inflamed muscle, and the impaired uptake of macrophages to PKH67 + apoptotic cells transferred into damaged muscle. Further, our study suggested that, the intrinsic TGF-β signaling directed IL-10-Vav1-Rac1 efferocytosis signaling in muscle macrophages. Conclusions Our data demonstrate that muscle inflammation can be suppressed potentially by activating the intrinsic TGF-β signaling in myofibers to promote IL-10 dependent-macrophages efferocytosis.
Video Abstract
研究领域:
免疫学
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肌肉生物学
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细胞信号传导
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炎症
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DOI
PubMed
高影响因子
Neural Regeneration Research
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2025
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IF 6.7
Gu Xinyi,Huang Chen,Wang Shen,Deng Jin,Guo Shuhang,Yin Xiaofeng
To perform various functions in the body, skeletal muscle is controlled and coordinated as a whole by nerves. However, there has been little research into whether the nerve control characteristics of different muscles are different, and the importance of these potential differences. In the present study, we used a three-dimensional imaging of solvent-cleared organ-compatible multi-tracer technique to explore the spatial distribution patterns of sensory and sympathetic neurons that innervate limb muscles. We integrated transcriptome sequencing datasets from mouse limb muscles in public databases and performed correlation analysis with neuronal spatial distribution data to reveal the unique effects of different types of neurons on muscle functional pathways. In terms of spatial distribution patterns, sympathetic neurons exhibited a more concentrated distribution than sensory and motor neurons. In addition, the neuronal innervation of limb muscles exhibited four different characteristics: sympathetic neuron-rich muscle, sensory neuron-rich muscle, neuron-sparse muscle, and motor neuron-rich muscle. Sensory neuron density was mainly associated with muscle contractile structure and cell pH, whereas sympathetic neuron density was associated with protein kinase activity, muscle vasculature, muscle calcium-dependent protein kinase activity, lipid transport, and vesicle release. Motor neuron density was mainly associated with protein kinase activity, cell adhesion, oxidoreductase activity, and exocytosis. These findings may contribute to a deeper understanding of how nerves cooperate to endow muscles with diverse physiological functions, thereby providing new insights and experimental evidence for the treatment of various neuromuscular diseases.
研究领域:
神经科学
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肌肉生理学
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系统生物学
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分子生物学
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解剖学
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DOI
PubMed
高影响因子
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
·
2025
·Vol. 39
·期 9
·e70449
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IF 2.8
Dai Lizhi,Yu Tong,Wang Jingxuan,Li Youchong,Cao Zheyuan,Xiao Jianhua
2-ethylhexyl diphenyl phosphate (EHDPHP), a ubiquitous organic phosphorus flame retardant, is extensively employed in food packaging, flame retardancy applications, and other industrial processes as a crucial chemical. However, recent discoveries have underscored its diverse toxic effects, posing a severe potential threat to biological health. Skeletal muscle, vital for locomotion, relies heavily on proper differentiation during development and maintenance for strength and functionality. This study investigated the impact of EHDPHP on skeletal muscle differentiation by culturing C2C12 cells with varying concentrations of EHDPHP during induction of differentiation, as well as constructing a mouse muscle injury repair model through oral administration of 30 μg/kg EHDPHP for 7 weeks. Exposure to EHDPHP was found to impede skeletal muscle differentiation in mice. Utilizing techniques such as western blot analysis, RNA-seq, and qRT-PCR, our preliminary analysis revealed that low concentrations of EHDPHP inhibited the expression of ACTC1, subsequently suppressing MyoG and MYH2 expression levels, thereby hindering C2C12 myoblast differentiation. Furthermore, EHDPHP exposure disrupted the repair process of muscle injury in mice and compromised the integrity of the repaired tissue. In conclusion, these findings elucidate the detrimental effects of EHDPHP exposure on muscle differentiation, uncovering its muscle toxicity.
研究领域:
毒理学
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分子生物学
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细胞生物学
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药理学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Yun-Fei Zhu,Xing-Yue Zhou,Cai Lan,Yong-Ping Wen,Hai-Jun Fu,Zhi-Chao Li,Ya-Ping Li,Shi-Ying Li,Fei-Hong Huang,Long Wang,Lu Yu,Da-Lian Qin,An-Guo Wu,Jian-Ming Wu,Xiao-Gang Zhou
Aging leads to progressive decline in the functions of cells, tissues, and organs, severely affecting muscle performance and overall health, highlighting the urgent need for effective therapeutic agents. This study investigated the antiaging properties of tricin, a flavonoid abundant in grains, using biological models, including human fibroblasts, Caenorhabditis elegans (C. elegans), and mice. Tricin significantly alleviated the senescent phenotype in human fibroblasts induced by D-galactose (D-gal), doxorubicin, and replicative senescence, as evidenced by reduced SA-β-gal activity, downregulated senescence markers (p16, p21), and decreased SASP factors. Mechanistically, tricin binds to AMPK and activates the AMPK-mTOR-p70S6K signaling pathway, promoting autophagy and delaying cellular aging. In vivo, tricin extended lifespan, enhanced stress resistance, and improved mobility in C. elegans through aak-2/AMPK-mediated autophagy. In D-gal-induced aging mice, tricin improved muscle function, reducing p16, p21, and SASP expression in muscle tissues. These findings underscore tricin’s potential as a promising antiaging therapeutic via AMPK-mediated autophagy activation.
研究领域:
细胞生物学
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分子生物学
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药理学
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DOI
PubMed
高影响因子
Journal of Translational Medicine
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2022
·Vol. 20
·期 1
·1-10
·
IF 8.44
Huang Kun,Wang Dan-Dan,Hu Wen-Bao,Zeng Wei-Qian,Xu Xia,Li Qiu-Xiang,Bi Fang-Fang,Yang Huan,Qiu Jian
Background Myotonic dystrophy type 1 (DM1), one of the most common forms of adult-onset muscular dystrophy, is caused by abnormally expanded CTG repeats in the 3′ untranslated region of the DMPK gene. The CUG repeats transcribed from the expanded CTG repeats sequestrate a splicing factor, MBNL1, causing the clinical symptoms in DM1. Nowadays, only symptomatic treatments are available for DM1, and no rational therapy is available. Recently, upregulation of MBNL1 expression has been found to be one of the promising therapies for DM1.Methods All experiments were conducted in the C2C12 myoblasts and HSALR mice, a DM1 mouse model. Real-time PCR and western blot were used to detect the mRNA and protein level, respectively. The rotarod exercise, grip strength and hanging time were used to evaluate the muscle strength of mice.Results In this study, we demonstrated that calcitriol, an active form of vitamin D3, increased MBNL1 in C2C12 mouse myoblasts as well as in HSALR mice model for DM1. In HSALR mice model, calcitriol improved muscle strength, and corrected aberrant splicing in skeletal muscle. Besides, calcitriol reduced the number of central nuclei, and improved muscle histopathology in HSALR mice. In addition, we identified that calcitriol upregulated MBNL1 expression via activating the promoter of Mbnl1 in C2C12 myogenic cells.Conclusion Our study suggests that calcitriol is a potential pharmacological strategy for DM1 that enhances MBNL1 expression.
研究领域:
分子生物学
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神经病学
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药理学
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肌肉骨骼疾病
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Lei Liu,Cheng Wang,Ze Wu,Youqiang Xing
The main challenge in manufacturing an ionic actuator of large bending displacement and great response sensitivity is to design a flexible electrode with great electrochemical characteristics and conductivity. This research reports the MXene-PEDOT:PSS/AgNWs (MPA) electrode with a three-dimensional (3D) network structure formed by a hybrid method of the one-dimensional (1D) silver nanowires (AgNWs) and the two-dimensional (2D) Ti3C2Tx MXene. Here, a soft actuator based on the ionic cross-linked hybrid electrode was designed. The results show that the MPA electrode-based soft actuator achieves a large bending strain (0.48%, ±0.5 V sine voltage), wide frequency (0.1–10 Hz), 5 h durability (91.9% retention), fast response time (≈5 s), great power density (7.53 kW m–3), and great energy density (18.83 kJ m–3). These excellent performances contribute to the 3D structure of electrodes formed by MXene and AgNWs creating an unhindered ion channel, which facilitates short diffusion and rapid injection of ions and provides higher capacitance and mechanical integrity. This 3D network layered structure hybrid electrode provides an opportunity for the development of ultralow-voltage-drivable artificial muscles.
研究领域:
材料科学
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生物医学工程
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Zhigang Ke,Yiwen Bai,Hao Zhu,Xingwei Xiang,Shulai Liu,Xuxia Zhou,Yuting Ding
In this study, the degradation of myoglobin both in solution and in tuna muscle by cold plasma (CP) was investigated through ultraviolet–visible and fluorescence spectroscopy analysis, and the effect of the degradation products on lipid oxidation in washed turbot muscle (WTM) was explored. Results showed that heme in myoglobin was degraded upon CP treatment, from which a fluorescent product was formed and iron was released. The degradation products promoted lipid oxidation in WTM during cold storage, in which the released iron played an important role. Results of gas chromatography-ion mobility spectrometry analysis showed that the contents of 37 volatile compounds in WTM were increased by heme degradation products induced lipid oxidation, including 20 aldehydes, 9 alcohols, 5 ketones and 3 furans. This study provides a new insight to the mechanism of lipid oxidation in CP-treated fish during cold storage, as well as guidance for preserving myoglobin-containing foods with CP.
研究领域:
食品科学
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化学
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生物学
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产品关联
DOI
PubMed
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
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2025
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IF 3.7
Yanxue Zhao,Xinpei Liu,Zining Wu,Guotao Ma,Quanli Gao,Jun Zheng,Chaoji Zhang
Rhabdomyosarcoma (RMS), a common malignant tumor in children, presents numerous challenges in clinical treatment. This study investigated the specific functions and regulatory mechanisms of distal-less homeobox 5 ( DLX5 ) in RMS. Data from TCGA, GEO and GEPIA databases were downloaded and analyzed. The effect of DLX5 and PAX3-FOXO1 on RMS cells was examined through cellular experiments. Binding activity between DLX5 and H3K9me2 was assessed using pull-down and chromatin immunoprecipitation-qPCR assays. Additionally, RMS model mice were constructed via xenotransplantation to validate the in vivo effects of DLX5 on RMS. The results revealed that DLX5 was upregulated in RMS tissues and increased in various RMS cell lines, particularly in alveolar RMS cell lines. DLX5 knockdown inhibited malignant biological behaviors. Besides, DLX5 expression was associated with myogenic differentiation of RMS cells. While the overexpression or knockdown of DLX5 did not affect PAX-FOXO1 expression. PAX3-FOXO1 knockdown reduced DLX5 expression, indicating that DLX5 act as a downstream effector of PAX3-FOXO1 . Mechanistically, PAX3-FOXO1 regulated DLX5 expression through KDM4B/H3K9me2 axis. In vitro experiments further demonstrated that knockout of DLX5 or KDM4B inhibited tumor growth. In conclusion, DLX5 expression was increased in PAX3-FOXO1 –driven RMS, and its knockdown inhibited malignant biological behaviors of RMS cells. Moreover, the aberrant expression of DLX5 in PAX3-FOXO1 –driven RMS was regulated by KDM4B/H3K9me2 axis. These findings provided potential therapeutic targets for RMS treatment.
研究领域:
分子生物学
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肿瘤学
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表观遗传学
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DOI
PubMed
高影响因子
AQUACULTURE
·
2018
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IF 2.71
Li Wang,Xuezhen Zhang,Lei Wu,Qi Liu,Dianfu Zhang,Jiaojiao Yin
Selenium (Se) is an essential trace element for fish growth and performs its physiological functions mainly through incorporating into selenoproteins . It is well known that dietary Se regulates fish growth by controlling the synthesis of deiodinase , a kind of selenoproteins. However, recently, as many as 41 selenoproteins have been characterized in teleost fish. We propose a hypothesis if other selenoproteins, besides deiodinase, could also involve in the regulation of fish growth. In the present study, rainbow trout ( Oncorhynchus mykiss ) were fed diets supplemented with or without graded levels of Se (2, 4 or 6 mg/kg, from selenium yeast, Se-yeast) for 10 weeks. At the end of the feeding trial, fish growth and the expressions of a total of 28 selenoprotein genes in tissues have been evaluated. Results showed that dietary Se-yeast supplementation significantly increased fish growth ( P < 0.05) without causing oxidative stress in fish tissues. In addition, dietary Se supplementation caused a general up-regulation of selenoprotein gene expressions either in liver (10 genes) or in muscle (11 genes) ( P < 0.05). Correlation analysis showed that rainbow trout growth was significantly and positively correlated to only 4 selenoprotein genes in liver but all the 11 differentially expressed selenoprotein genes in muscle ( P < 0.05), and it presented the strongest correlation with the mRNA levels of muscle selenoprotein W-like gene ( P < 0.05). These results indicate that dietary Se-yeast supplementation is beneficial for rainbow trout growth, and the enhanced growth performance is closely correlated to the expressions of muscle selenoprotein genes, particularly the selenoprotein W-like gene. This study reveals the importance of muscle selenoprotein gene expressions, and provides a new concept for the regulatory mechanism of dietary Se on fish growth.
研究领域:
水产养殖学
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分子生物学
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营养学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）