MATERIALS CHEMISTRY AND PHYSICS
·
2021
·
IF 4.094
Rongrong Xue,Gaoxiang Ma,Huifang Ye,Nuo Man,Yuezong Lian,Wangchuan Xiao,Fenghua Chen
Guanosine (GR) dihydrate and inosine (IR) dihydrate have similar crystal structure, water molecules are almost at the same locations in them. GR dihydrate has the reversible dehydration/hydration property and is a good candidate for materials harvesting water from atmosphere. IR dihydrate is not stable and can be easily transformed to anhydrous IR α form. In this work, doping of IR in GR dihydrate in all the range was realized for the first time by using a solution method. The successful preparation of doped dihydrate of IR and GR by the solution method was attributed to the similar molecular conformations of IR and GR in solution. The doped dihydrates could maintain the reversible dehydration/hydration property similar to pure GR dihydrate even the ratio of IR was up to 71.1 ± 1.2 mol% under a mild drying treatment with freeze-dry. A pure amorphous GR was prepared by ball milling, and it showed a similar dehydration/hydration property to GR dihydrate with a lower dehydration temperature (about 84 °C). The implementation of doping IR in GR dihydrate would shed light on the design and fabrication of functional doped organic crystals. And study on the structural insights and the dehydration/hydration processes of the doped dihydrates and the pure amorphous GR would shed light on the design and fabrication of novel hydrogen-bonded organic frameworks (HOFs) materials for water harvesting.
研究领域:
化学
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材料科学
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产品关联
DOI
高影响因子
Food Science and Human Wellness
·
2022
·
IF 8.022
Hongyuan Zhao,Xiaoyu Chen,Fanqiang Meng,Libang Zhou,Xinyi Pang,Zhaoxin Lu,Yingjian Lu
Hyperuricemia is a critical threat to human health, and a high inosine diet can increase the prevalence of it. Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt, and its inosine degradation rate reached 36.3 % at 10 9 CFU/mL for 24 h. LC-MS analysis revealed that high concentrations of inosine could activate compensatory metabolic pathways of L. rhamnosus Fmb14 to catalyse inosine as an energy source and produce intracellular folic acid and riboflavin. The contents of folic acid and riboflavin were 6.0 and 4.3 fold increased after inosine treatment in the cell-free extracts (CFE). L. rhamnosus Fmb14 CFE treatment ameliorates hyperuricemia through xanthine oxidase (XOD) inhibition and ATP-binding cassette subfamily G member 2 (ABCG2) promotion, both of which are responsible for uric acid (UA) synthesis and secretion in HepG2 and Caco2 cells, respectively. The in vivo results showed that the serum UA level decreased from 236.28 to 149.28 μmol/L after 8 weeks of oral administration of L. rhamnosus Fmb14 in inosine-induced hyperuricemia model mice. Our results revealed that L. rhamnosus Fmb14 has a potential as a biological therapeutic agent in hyperuricemia prevention.
研究领域:
微生物学
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药理学
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分子生物学
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营养学
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产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2020
·Vol. 44
·期 5
·954-962
·
IF 2.878
Lu Kang,Ju Liu,Hongyang Zhang,Min Jiang,Yidian Jin,Min Zhang,Ping Hu
Creatinine and purines are gout-related metabolites commonly quantified by liquid chromatography coupled with ultraviolet and mass spectrometry. However, the high cost of liquid chromatography coupled with mass spectrometry hindered its extensive use in ordinary hospitals and clinical laboratories. Using the traditional liquid chromatography method, the full separation of these metabolites in complex biological samples is still not achieved. In this study, an improved ultra-high-performance liquid chromatography with ultraviolet spectroscopy method was reported for quantitative determination of five gout-related metabolites (i.e., creatinine, uric acid, hypoxanthine, xanthine, and inosine) in human serum within 10 min. A UHPLC system equipped with a hydrophilic C18 column was used to improve separation, shorten analysis time, and increase analysis throughput. The performance of the method was validated by evaluating linearity (squared correlation coefficient > 0.9991), recovery (92.8–100.0%, with relative standard deviation < 4.7%), accuracy (relative errors < 14.6%), precision (0.2-4.1% for intraday and 2.1-7.3% for interday) and stability (-14.1 to 8.3% in autosampler for 12 h and -13.3 to 2.2% for freeze-thaw cycles). This method was successfully applied to quantify gout-related metabolites in serum samples of healthy controls and gout patients, which was expected to be used in the clinical investigation of gout at different stages.
研究领域:
医学检验
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代谢组学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Ben Wu,Imre Blank,Yin Zhang,Yuan Liu
Three types of tastants are known as perceptually associated with umami taste: monosodium glutamate (MSG), disodium succinate (WSA), and disodium inosine monophosphate (IMP). While these tastants were confirmed to be perceptually similar in a sensory study, they could be discriminated (p < 0.05) by electroencephalogram (EEG) analysis on a time scale of 5–6 s. In comparison of the EEG responses of the participants, the brain could partly distinguish (p < 0.05) between different sensory intensities of MSG, WSA, or IMP. The EEG data indicated that the brain is partially sensitive to perceiving different sensory intensities (L, low; M, medium; and H, high) of the same umami stimuli; i.e., for MSG in μV2/Hz, L, 2.473 ± 0.181; M, 3.274 ± 0.181; and H, 3.202 ± 0.181. However, brain responses of perceptually equi-umami intensities could partially be discriminated, suggesting that the brain could partially discriminate (p < 0.05) MSG, WSA, and IMP, despite similar sensory intensities. Moreover, umami tastants were also found to significantly enhance (p < 0.05) the α wave activity, with the most responsive being at 10 Hz, particularly in the frontal and parietal and occipital regions of the brain (p < 0.001). This study shows the potential of EEG to investigate brain activity triggered by umami stimuli.
研究领域:
神经科学
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食品科学
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感官研究
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2025
·Vol. 39
·期 4
·e70038
·
IF 1.7
Mengjiao Li,Fuqiang Liu,Lulu Guo,Wu Fan,Jiazhong Wang,Binbin Lu,Guangfeng Hong,Wenjuan Zhang,Shu Tian,Jian Mao,Jianping Xie
Cordycepin, a natural adenosine derivative, exhibits multiple pharmacological effects on organisms. However, its distribution and metabolic characteristics have not been fully elucidated in vivo. In this study, ultra-high liquid chromatography tandem high-resolution mass spectrometry (UHPLC-HRMS/MS) was used to investigate the pharmacokinetic characteristics and effects of cordycepin on endogenous adenosine and inosine. Microdialysis was used for real-time monitoring of unbound drug in brain and blood, whereas conventional tissue homogenate methods assessed distribution in various tissues. Results showed that the distribution pattern of cordycepin was as follows: kidney > liver > heart > lung > spleen > brain. Cordycepin administration significantly altered the levels of adenosine and inosine in heart and liver. Synchronous microdialysis sampling for the pharmacokinetic profile indicated that cordycepin was rapidly consumed and 3′-deoxyinosine was generated as the main metabolite. The C max values of cordycepin in the rat blood and brain after exposure (10 mg/kg, i.p.) were 7.8 and 5.4 ng/mL, respectively. Mean residence time in blood and brain was 102.2 and 137.0 min, respectively. Inhibition of adenosine deaminase by racemic 9-(2-hydroxy-3-nonyl) adenine hydrochloride (EHNA) enhanced cordycepin levels in the blood. This work provides a solid basis for understanding the metabolism of cordycepin and its pharmacological effects.
研究领域:
药理学
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分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 29
·18301-18306
·
IF 4.036
Fenghua Chen,Chenmei Yang,Xinyu Cheng,Yingjie Fan,Xinyuan Chen,Shizhao Ren,Rongrong Xue
Mid-frequency Raman difference spectra (MFRDS) analysis can be used to reveal the selective crystallization from solutions through determining the degree of similarity of the short-range orders between the assemblies of small organic molecules in solutions and their solid phases. Four solid phases of inosine (IR) (α-anhydrous IR (α-IR), β-anhydrous IR (β-IR), IR dihydrate (IRD), and amorphous IR (AmIR)) and two IR solutions (aqueous and 70 vol% DMSO aqueous solution) were prepared and characterized using MFRDS here. The MFRDS analysis results indicate that the selective formation of IRD and AmIR from IR aqueous solution and β-IR from IR 70 vol% DMSO solution are originated from the high similarity of their short-range structures. Moreover, we propose that the formation of α-IR from IR aqueous solution benefits from the appearance of AmIR as an intermediate phase. MFRDS is a robust tool to explain and predict the possible precipitation products from various solutions of small organic molecules.
研究领域:
化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Heliyon
·
2023
·
IF 4
Wei Liu,Yi Zhu,Hong Yan,Lingyun Ren,Jingli Chen
Objective To study the effect of nicotine in rat model of pneumonia induced by polyinosinic-polycytidylic acid [Poly (I:C)] and explore the underlying mechanism. Methods Twenty-four healthy adult male Sprague-Dawley (SD) rats (200–250 g) were randomly divided into normal saline control group (NS group); Poly (I:C) group; nicotine group (NIC group); and α7 nicotinic acetylcholine receptor (α7nAChR) antagonist group (α-BGT group) (n = 6 each). Rats in the Poly (I: C), NIC, and α-BGT groups were administered 1.5 mg/mL 100 μL Poly (I:C) intranasally to establish pneumonia model. In α-BGT group, 1 μg/kg α-bungarotoxin (α-BGT) was intraperitoneally injected 45 min before intranasal Poly (I:C), and 400 μg/kg nicotine was intraperitoneally injected 15 min after α-BGT injection. The NIC group received an equal volume of NS in place of α-BGT while the other treatments were same. The Poly (I:C) group received equal volume of NS in place of nicotine while the other treatments were same as in NIC group. In the NS group, only NS was administered at all three time points. PaCO 2 , PaO 2 , and PaO 2 /FiO 2 levels were determined 24 h after administration of Poly (I:C). After euthanization, rat lung tissues were extracted for pathological examination, and wet weight/dry weight (W/D ratio) was determined. Expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β, and interferon (IFN)-γ in lung tissue was determined by ELISA. q-PCR was used to detect nuclear factor kappa-B P65 (NF-κBP65). Results Compared with NS group, Poly (I:C) and α-BGT groups showed significantly increased W/D ratio, PaCO 2 , TNF-α, IL-6, IL-1β, and IFN-γ content, NF-κB P65 expression, and reduced PaO 2 and PaO 2 /FiO 2 ( p < 0.05), along with obvious signs of pathological injury. Nicotine pre-treatment reduced W/D ratio, PaCO 2 , proinflammatory cytokines, NF-κBP65 expression, and increased PaO 2 and PaO 2 /FiO 2 levels. The above effects were negated in α-BGT group. Conclusion Pre-administration of nicotine improved Poly (I:C)-induced pneumonia by activating the cholinergic anti-inflammatory pathway.
研究领域:
药理学
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免疫学
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呼吸医学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Chong Sun,Ling Gao,Daoying Wang,Muhan Zhang,Yuan Liu,Zhiming Geng,Weimin Xu,Fang Liu,Huan Bian
Inosine monophosphate (IMP) is considered to be an important constituent in the formation and development of meat flavor. In this paper, a bi-enzyme biosensor system for determination of IMP was developed to evaluate the freshness and quality of stored muscles. More details of preparing for polypyrrole-Pluronic F127-gold nanoparticles (PPy-F127-Au NPs) and immobilizing bi-enzyme (peroxidase and 5′-nucleotidase) on PPy-F127-Au NPs modified glassy carbon electrode (GCE) were presented. The electrochemical behaviors of the bi-enzyme biosensor were analyzed, and the concentration of IMP was determined based on the bi-enzyme biosensor response. The results of linear sweep voltammetric (LSV) and square wave voltammetry (SWV) indicated that the catalytic reaction was occurred favorably on the surface of the bi-enzyme biosensor, with a wide detection range (0.05–15 g/L) and a low detection limit of 3.85 ng/mL. The good anti-interference ability, stability and repeatability of this biosensor were also proved. The analytical results by the biosensor system did correlate well with those obtained by liquid chromatography.
研究领域:
生物传感器技术
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食品质量评估
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电化学分析
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生物医学工程
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纳米材料应用
✓ 已验证
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Lijun Tan,Shasha Zheng,Yong Xie,Wendi Zhang,Baocai Xu
Photodynamic inactivation (PDI) is a promising non-thermal antimicrobial technology known for broad-spectrum efficacy and low resistance potential. However, conventional PDI face limitations in bactericidal efficacy due to the short-lived and poorly diffusing reactive oxygen species (ROS). This study proposes a novel food-grade PDI strategy that incorporates disodium 5′-inosinate (IMP) into a vitamin B2 (VB2)-mediated system, significantly enhancing H 2 O 2 production. Acting as an electron donor, IMP promotes type I photochemical reactions, enabling sustained H 2 O 2 generation and achieving >6 log CFU/mL inactivation of Bacillus safensis , a spoilage bacterium often overlooked in cooked meat products. Mechanistic analyses showed that H 2 O 2 oxidized key phospholipids in the cell membrane, increased permeability, caused membrane potential hyperpolarization and ion leakage, and ultimately disrupted multiple membrane domains. Moreover, this strategy also exhibited persistent antimicrobial activity, effectively delaying spoilage in cooked meat products stored at 4 °C and 25 °C. Overall, this study introduces a safe and effective non-thermal sterilization method with promising industrial potential and offers a novel pathway to enhance PDI efficacy via intensified type I ROS mechanisms.
研究领域:
食品科学
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光动力学
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微生物学
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生物化学
·
食品保鲜技术
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产品关联
DOI
高影响因子
INSECT MOLECULAR BIOLOGY
·
2023
·
IF 2.6
Zhi-hao Su,Jun-li Lv,Qi Ou,Zi-qin Zhao,Kai-yi Zheng,Xiao-ying Zhang,Wen-qing Lai,Xue-yang Wang,Ming-jie Deng,Mu-wang Li
The white epidermis of silkworms is due to the accumulation of uric acid crystals. Abnormal silkworm uric acid metabolism decreases uric acid production, leading to a transparent or translucent phenotype. The oily silkworm op50 is a mutant strain with a highly transparent epidermis derived from the p50 strain. It shows more susceptibility to Bombyx mori nucleopolyhedrovirus (BmNPV) infection than the wild type; however, the underlying mechanism is unknown. This study analysed the changes in 34 metabolites in p50 and op50 at different times following BmNPV infection based on comparative metabolomics. The differential metabolites were mainly clustered in six metabolic pathways. Of these, the uric acid pathway was identified as critical for resistance in silkworms, as feeding with inosine significantly enhanced larval resistance compared to other metabolites and modulated other metabolic pathways. Additionally, the increased level of resistance to BmNPV in inosine-fed silkworms was associated with the regulation of apoptosis, which is mediated by the reactive oxygen species produced during uric acid synthesis. Furthermore, feeding the industrial strain Jingsong (JS) with inosine significantly increased the level of larval resistance to BmNPV, indicating its potential application in controlling the virus in sericulture. These results lay the foundation for clarifying the resistance mechanism of silkworms to BmNPV and provide new strategies and methods for the biological control of pests.
研究领域:
昆虫学
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分子生物学
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病毒学
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代谢组学
✓ 已验证
DOI
PubMed
高影响因子
CLINICA CHIMICA ACTA
·
2018
·
IF 2.926
Feiyan Liu,Luyang Xu,Changcheng Sheng,Xiaoyan Qiu,Ming Zhang,Zheng Jiao
Background The immunosuppressive agent mycophenolic acid (MPA) is a non-competitive, reversible inhibitor of inosine-5′-monophosphate dehydrogenase (IMPDH). Thus, IMPDH activity can serve as a potential pharmacodynamic biomarker to optimize dosing of MPA. Methods Peripheral blood mononuclear cells were isolated from 2 mL blood samples and an in vitro enzymatic reaction was subsequently performed for 120 min. To determine IMPDH activity in Chinese healthy volunteers and renal transplant patients, a high performance liquid chromatography assay was established and validated by subtracting adenosine monophosphate (AMP) from blank samples for eliminating exogenous AMP interference. Results The accuracy of our method ranged between −0.8% and 12.5%, and the precision ranged between 0.7% and 6.3%. The mean value of IMPDH activity across 11 healthy volunteers was 46.60 ± 14.28 μmol/s/mol AMP. A negative relationship between MPA concentration and IMPDH activity was observed in four renal transplant patients treated with MPA 13 days post-transplantation, while the inhibitory rate of IMPDH activity ranged from 24% to 42%. Conclusion A bioanalytical assay for IMPDH quantification was optimized and evaluated. The differences in the pharmacodynamics of MPA between Asians and Caucasians may provide some evidence for dosing differences among ethnicities.
研究领域:
药理学
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临床医学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2021
·
IF 2.329
Lei Xu,Jing Lu,Weiqi Meng,Fengjuan Cui
Micellar electrokinetic chromatography (MEKC) with ultraviolet detection was employed for the simultaneous determination of the nucleoside reverse transcriptase inhibitors lamivudine, stavudine and didanosine with the ionic liquid-based cationic surfactant 1-tetradecyl-3-methylimidazolium bromide ([C14MIm]Br) as the pseudostationary phase. The composition, pH, and concentration of running buffer, surfactant concentration, separation voltage, and injection time were optimized. In comparison with conventional cationic surfactants, improved separation was observed using [C14MIm]Br. The applicability of the method was demonstrated by the analysis of human urine. The method exhibited wide linear ranges with limits of quantitation of 0.5, 0.3, and 1.0 μg/mL for lamivudine, stavudine, and didanosine, respectively. The intra-day and inter-day accuracies were from 91.5% to 114.0%. The precision, expressed as relative standard deviation (RSD), was less than 12.6% for the peak area. The recoveries of the analytes in the samples were between 91.8% and 97.3%. This approach is convenient, rapid, and economical for these analytes in biological samples.
研究领域:
分析化学
·
医学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）