MICROPOROUS AND MESOPOROUS MATERIALS
·
2022
·
IF 5.876
Ning Wu,Hao Guo,Liping Peng,Yuan Chen,Lei Sun,Yinsheng Liu,Xiaoqing Wei,Wu Yang
Creatinine (Cre) measurement in biological fluids is an increasingly important clinical measure for evaluating renal insufficiency, thyroid dysfunction, and muscle injury. Therefore, a simple, fast, and reliable Cre analysis method is of great significance to human health. Herein, a novel ratiometric fluorescence sensor, Ag-Eu-MOF-808-EDTA, was synthesized by introducing ethylenediaminetetraacetic acid (EDTA), Ag + , and Eu 3+ into the framework of MOF-808 through three steps of post-synthesis modification, and was used to detect Cre in PBS buffer of pH 7.2 at 35 °C with a “turn-on” ratio response in the presence of various interfering ions and several biomolecules. In this strategy, the characteristic emission of Eu 3+ at 551 nm can be turned on due to coordination between Cre and Ag + , while emission at 613 nm is independent of Cre, which produces a significant ratio response change throughout the cycle. Experimental results showed that the ratio fluorescence sensor displayed a wide linear range for Cre from 0.5 to 350 μM with a low detection limit of 0.42 μM. Finally, a good recovery rate (96.7–102.9%) and appropriate relative standard deviation (RSD <2.39%, n = 3) were obtained during the determination of creatinine in real urine and serum. This work provides additional insights into the detection of creatinine in clinical blood samples.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
Plasmonics
·
2024
·
IF 3.3
Lv Xianfa,Shi Tongrui,Bai Xia,Guan Zheng,Wang Huanran,Jiang Rujian,Zhou Lu,Chen Hongyu
Creatinine level is a crucial indicator in the clinical assessment and diagnosis of renal diseases, and achieving simple and accurate detection of urinary creatinine levels in resource-limited point-of-care settings is of great significance in the timely prevention and diagnosis of kidney diseases. As a popular zero-dimensional material, gold nanoparticles (AuNPs) exhibit intriguing optical properties and thus have become a promising material for many sensing detection applications. Here, we proposed a simple, efficient, and sensitive quantitative detection of creatinine by studying the relative absorbance (Δ A ) of AuNPs in absence and presence of creatinine. The method relies on the aggregation of AuNPs via ligand-exchanged of citrate ions and creatinine on the surface of AuNPs to achieve colorimetric detection. With this assay, the limit of detection for creatinine was as low as 0.16 mM, and the dynamic detection range was 0.5 to 20 mM under optimized conditions. In our experiments, the specificity of the proposed method was investigated and successfully applied to detect creatinine in urine sample. It reveals that the proposed colorimetric protocol has demonstrated a high sensitivity and selectivity for creatinine, and has a potential practicability in clinical diagnostics.
研究领域:
医学诊断
·
纳米材料科学
·
临床化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 182
·期 11
·2037-2043
·
IF 3.741
He Yi,Zhang Xianhui,Yu Haili
We demonstrate a selective and sensitive method for determination of creatinine using citrate-stabilized gold nanoparticles (AuNPs) as a colorimetric probe. It is based on a direct cross-linking reaction that occurs between creatinine and AuNPs that causes aggregation of AuNPs and results in a color change from wine red to blue. The absorption peak is shifted from 520 to 670 nm. Under the optimized conditions, the shift in the absorption peak is related the logarithm of the creatinine concentration in the 0.1 to 20 mM range, and the instrumental detection limit (LOD) is 80 μM. This LOD is about one order of magnitude better than that that of the Jaffé method (720 μM). The assay displays good selectivity over interfering substances including various inorganic ions, organic small compounds, proteins, and biothiols. It was successfully employed to the determination of creatinine in spiked human urine.
The colorimetric assay for creatinine uses citrate-stabilized gold nanoparticles (AuNPs) and a direct cross-linking reaction that occurs between creatinine and AuNPs that causes aggregation of AuNPs and results in a color change from wine red to blue.
研究领域:
生物化学
·
纳米医学
·
诊断学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Haijing Tian,Ruo Wu,Qi Lv,Jie Shi,Xiaoqin Guo,Duo Li,Chunli Liu,Qi Li,Weijia Zheng,Haojun Fan,Xuexin Duan,Qiannan Xue
Blood potassium and creatinine levels are critical indicators for managing chronic kidney disease (CKD). In response to the need for home monitoring and emergency care, point-of-care diagnostic technologies based on microsystems have emerged, nevertheless they remain limited in portability and speed for direct in whole blood samples measurement. To enable direct blood detection of potassium and creatinine for effective CKD management, this study presents a novel on-chip self-driven dual-channel optical microsensor designed for real-time monitoring of potassium and creatinine levels in whole blood. Utilizing 3D printing technology to design and fabricate microstructures, and integrating microchannels with a filtration layer, this microsensor requires only 10 μL of blood for single-pass sample filtration and dual-parameter detection. A novel passive blood sample pumping method is proposed, utilizing internal and external pressure differentials along with internal hydrophilic properties to facilitate sample flow into the detection chambers. Light-emitting diodes (LEDs) and photodiodes, positioned on either side of the detection chamber, are employed to measure the concentrations of blood indicators. The detection results can be instantly handheld read and uploaded to the cloud via intelligent terminal, enabling seamless follow-up diagnostics and treatment. Through multiple rounds of testing on animal serum samples, whole blood samples, and real porcine blood samples, this microsensor demonstrated excellent linearity and accuracy. This innovative microsensor, designed for direct testing from a minimal volume of whole blood, holds significant potential for improving CKD management, offering a portable, reliable alternative for preoperative diagnostics and home-based chronic disease monitoring.
研究领域:
医学工程
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-11
·
IF 5.7
Lu Yuyang,Shen Nuotong,Xi Yachao,Zhu Tao,Peng Hao,Zhong Lihao,Li Fang
Mn 3 O 4 nanozyme with good oxidase-like activity was successfully synthesized. The prepared Mn 3 O 4 nanozyme can directly and effectively catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate green–blue-colored ox-TMB. Creatinine exhibits distinct inhibition effect on Mn 3 O 4 nanozyme-catalyzed TMB colorimetric reaction system, leading to obvious color fading and absorbance intensity decrease of the reaction system. Furthermore, interference from uric acid can be effectively eliminated by regulating the pH of TMB-Mn 3 O 4 colorimetric reaction system to pH 2.0. Then, a simple and bioenzyme-free colorimetric assay for the determination of creatinine was developed based on TMB-Mn 3 O 4 colorimetric reaction. The linear detection range is from 100 to 800 μM and from 1 to 20 mM. The lowest limit of detection is 35.3 μM. Satisfied results are obtained for the determination of creatinine in real urine and sweat samples. This work provides the synthesis of a good oxidase-like nanozyme Mn 3 O 4 and presents the fabrication of an effective nanozyme-based bioenzyme-free colorimetric assay for the determination of creatinine. Graphical Abstract
研究领域:
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomedical Optics Express
·
2018
·Vol. 9
·期 10
·4988-4997
·
IF 3.482
Yudong Lu,Changji Wu,Ruiyun You,Yang Wu,Huiying Shen,Lanjin Zhu,Shangyuan Feng
Superhydrophobic silver films were fabricated by silver-mirror reaction and surface functionalization with thiol. The thiol-functionalization significantly improved the hydrophobic property of the Ag films (AFS), and their contact angle values slightly increased with the extension of a thiol alkyl chain, reaching about 160°. The surface-enhanced Raman scattering (SERS) detection capacity of these films were investigated, and AFS-Dodec showed the best substrate for R6G molecule detection with the concentration limit of 10−11 M. AFS functionalized with dodecanethiol (AFS-Dodec) was applied for the SERS detection of uric acid and creatinine, it exhibited good linear dependence relationship between the Raman intensity and analyte concentration in the concentration range of 5~1000 μM.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Wei Zhu,Bao-Ying Wen,Ling-Jun Jie,Xiang-Dong Tian,Zhi-Lin Yang,Petar M. Radjenovic,Shi-Yi Luo,Zhong-Qun Tian,Jian-Feng Li
The creatinine concentration of human urine is closely related to human kidney health and its rapid, quantitative, and low-cost detection has always been demanded. Herein, a surface-enhanced Raman spectroscopic (SERS) method for rapid and cost-effective quantification of creatinine concentrations in human urine was developed. A Au nanoparticle solution (Au sol) was used as a SERS substrate and the influence of different agglomerating salts on its sensitivity toward detecting creatinine concentrations was studied and optimized, as well as the effect of both the salt and Au sol concentrations. The variation in creatinine spectra over time on different substrates was also examined, demonstrating reproducible quantitative analysis of creatinine concentrations in solution. By adjusting the pH, a simple liquid−liquid solvent extraction procedure, which extracted creatinine from human urine, was used to increase the SERS detection selectivity toward creatinine in complex matrices . The quantitative results were compared to those obtained with a clinically validated enzymatic “creatinine kit (CK).” The limit of detection (LOD) for the SERS technique was 1.45 mg L −1 , compared with 3.4 mg L −1 for the CK method. Furthermore, cross-comparing the results from the two methods, the average difference was 5.84% and the whole SERS detection process could be completed within 2 min compared with 11 min for the CK, indicating the practicality of the quantitative SERS technique. This novel quantitative technique shows promises as a high-throughput platform for relevant clinical and forensic analysis.
研究领域:
医学诊断
·
光谱学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Huijun Guo,Jie Chen,Siqi Li,Yifei Qi,Guoxin Wang,Yue Zhang
In this work, a ratiometric double signals electrochemical sensing platform was constructed for creatinine (Cre) analysis employing silver nanoparticles (AgNPs)@ MXenes/thionine acetate (THAT) composites. The synthesis of MXenes/THAT composites was utilized to modify on the surface of a screen-printed electrode (SPE) via dripping coating, followed by the electro-deposition of AgNPs. While the anodic current of the AgNPs decreased in the presence of Cre due to the competive reaction for forming metal complexes, the current of THAT remained invariant, which attained a ratiometric response. Under optimized conditions, the response current ratio ( I Ag /I THAT ) decreases while the concentration of Cre increased linearly between 0 ∼ 1.2 mM, with 0.01 μM as a adaptive detection limit. It provided a new sensing platforming for employing double-signals nanostructures with remarkable stability and sensitivity to design ratiometic sensors for analysis of Cre in human urine samples with real-time.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2016
·
IF 2.567
Hong Du,Ruiyi Chen,Jianjun Du,Jiangli Fan,Xiaojun Peng
A gold nanoparticle-based colorimetric sensor for the determination of creatinine was developed as an important index for early diagnosis of kidney function and corresponding renal diseases. Because of the unique synergistic coordination capability of adenosine and creatinine with Ag+ on a particle surface, our system exhibits an excellent selectivity to creatinine among various ions and biomolecules. There are good linear relationships of absorption changes (A630 nm/520 nm) over creatinine concentrations, so both colorimetric qualitative detection by the naked eye and quantitative determination by UV–vis spectrometer could be realized with an excellent limit of detection compared with that of other methods. Finally, by testing creatinine in practical samples, such as urine mimic and bovine serum, good recoveries were obtained with proper relative standard deviations.
研究领域:
生物化学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Yaping Dong,Xiaojin Luo,Yiqun Liu,Cunling Yan,Haixia Li,Jicheng Lv,Li Yang,Yue Cui
In clinical practice, sera creatinine level is regarded as a crucial biomarker for the diagnosis, staging and monitoring of kidney disease. An amperometric biosensor is rapid, accurate, and cost-effective, with a portability and a simple operation. Herein, we report for the firsttime a disposable, printed amperometric biosensor for the clinical evaluation of creatinine in renal function detection. The sensor is constructed based on Prussian blue/carbon-graphite paste as the working electrode and the immobilization of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. The creatinine biosensor shows a linear detection range from 0.05 to 1.4 mM with a detection time of about 3 min. In addition, the sensor shows a high stability that can maintain above 86% of the initial activity after being stored for over 4 months. Moreover, the sensor shows almost the same results as those with the Jaffe method for measuring the real blood samples. We anticipate that the creatinine biosensor could be widely used in the medical and healthcare areas, especially for at-home testing and onsite medical examinations.
研究领域:
临床化学
·
生物医学工程
·
医疗器械
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Weiqing Kong,Qingqing Dai,Junli Ren,Nianfang Ma
A new xylan ester (xylan 3,5-dinitrobenzoate) as a creatinine adsorbent was prepared by the homogeneous acylation of xylan with 3,5-dinitrobenzoyl chloride in 1-butul-3-methylimidazolium chloride ionic liquid. The influences of reaction conditions on the degree of substitution values of xylan esters were discussed. Results indicated that xylan esters with the degree of substitution range from 1.34 to 1.77 were obtained under the given conditions. The FTIR and 13 C NMR spectroscopies provided the evidence of grafting 3,5-dinitrobenzoyl groups onto the backbone of xylan. Moreover, the adsorption properties of the xylan ester for creatinine were also investigated. Isotherm studies showed that the sorption capacities for creatinine were 2.45, 2.08 and 1.86 mg/g for 23, 30 and 37 °C, respectively. Thermodynamic studies performed indicated the sorption process mainly was controlled by the chemical adsorption. Therefore, xylan 3,5-dinitrobenzoate displayed the promising application in the treatment of chronic renal failure by the creatinine adsorption as the new oral adsorbent.
研究领域:
生物化学
·
医学材料
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plasmonics
·
2016
·Vol. 11
·期 6
·1573-1578
·
IF 2.146
Zhang Xianhui,Zheng Lei,He Yi
We report a simple, facile, selective, and highly sensitive colorimetric sensor for nitroguanidine explosive. The colorimetric sensor utilizes the aggregation of creatinine-modified gold nanoparticles (AuNPs), which was caused by nitroguanidine via hydrogen bond interaction between nitroguanidine and creatinine, leading to a color change from red to blue to colorless dependent on the concentration of nitroguanidine. Under optimum sensing concentrations, the absorbance changes of AuNPs at 519 nm were linear with the concentration of nitroguanidine in the range of 15–55 μM with a correlation coefficient of 0.997. The limit of detection of the colorimetric sensor was 1 μM based on the signal-to-noise of three, which has improved by as much as five orders of magnitude as compared with the traditional volumetric method and up to three orders of magnitude as compared with fluorescence approach as well. Moreover, as low as 15 μM of nitroguanidine can be visually detected by the naked eye without instrumentation. The colorimetric sensor also displayed remarkable selectivity to nitroguanidine against other explosives and common inorganic salts. It was robust and can be employed for reliable sensing of spiked nitroguanidine in real water samples with good recovery and accuracy.
研究领域:
分析化学
·
纳米材料科学
·
爆炸物检测
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）