Jing Yang,Xiaoye Feng,Haiqin Li,Pengtao Chang,Xiaojun Ren,Xiaochun Li
Chronic kidney disease (CKD) has become a public health burden, with a high mortality rate and a trend toward youthfulness. It is imperative to develop a home monitoring system for the early diagnosis and long-term monitoring of CKD serum biomarkers. However, due to the limitations of conventional centrifugation preprocessing of whole blood samples, the real-time detection platform cannot fully meet the requirement of obtaining quantitative results directly from whole blood. Herein, a novel smartphone-assisted microfluidic chip integration of a whole blood separation point-of-care testing (POCT) system was proposed to achieve accurate quantification of CKD biomarkers uric acid (UA), creatinine (CR), and albumin (Alb) based on 30 μL of whole blood (fingertip blood). The whole blood separation module allows rapid separation of plasma from whole blood samples by vertical flow. A smartphone App for automated image recognition and processing was developed, which was applied for at-home routine healthcare. The detection limits of UA, CR, and Alb were 0.1127 mmol/L, 0.2978 μmol/L, and 0.7696 mg/mL, respectively, meeting clinical requirements. Additionally, the quantitative performance of 83 clinical whole blood samples showed high consistency with the clinical analyzer (Beckman AU 5400 automatic biochemical analyzer). This system is simple to operate and user-friendly, providing an accurate, rapid, cost-effective, and reliable detection method for the early screening and real-time monitoring of CKD, which holds significant potential in enhancing home self-management of CKD.
Dongshuai Shen,Ning Ma,Yajun Yang,Xiwang Liu,Zhe Qin,Shihong Li,Zenghua Jiao,Xiaojun Kong,Jianyong Li
Aspirin eugenol ester (AEE) is a novel compound that is formed from the esterification of aspirin (acetylsalicylic acid (ASA)) and eugenol. This study aimed to investigate the effects of AEE on blood stasis in rats and to characterize the underlying mechanisms using a plasma metabolomic study. The results indicate that AEE and ASA could modulate whole blood viscosity (WBV), plasma viscosity (PV), blood coagulation parameters, platelet count, platelet aggregation, lactate dehydrogenase (LDH), creatinine (CR) and the levels of thromboxane A2 (TXA2) and 6-keto prostaglandin F1α (6-keto-PGF1α). The metabolic profiles of the plasma samples from all groups were clearly separated in the score plots. Nineteen potential metabolites were selected and identified, and disordered levels of these metabolites could be regulated by AEE and ASA. Pathway analysis showed that the mechanism of action of AEE on blood stasis might be principally related to the metabolism of amino acid, fatty acid, energy and glycerophospholipid. The above results indicate that AEE protected the rats against blood stasis, and that this effect might have been caused by the anticoagulation activity of AEE and its abilities to maintain a balance between TXA2and PGI2, reduce blood viscosity, inhibit platelet aggregation and normalize the plasma metabolic profile.Keywords:blood stasis;AEE;aspirin;metabolomics;UPLC-Q-TOF/MS
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