ENVIRONMENTAL RESEARCH
·
2020
·
IF 5.715
Jun Zhu,Lei Tang,Shanlei Qiao,Lijuan Wang,Yiming Feng,Li Wang,Qian Wu,Ping Ding,Zhan Zhang,Lei Li
Methylmercury (MeHg) is a ubiquitous environmental toxicant with neurotoxic effects. Although its neurotoxicity had been more studied, the role of gut microbiota remains unclear. In this study, adult zebrafish and larvae were exposed to MeHgCl at the dose of 0, 1 and 10 ng/mL. MeHgCl exposure impaired the locomotor activity via upregulation of apoptosis and autophagy related genes in the brain. Intestinal and cerebral metabolome indicated that phosphatidylinositol signaling system and inositol phosphate metabolism pathways were significantly impacted in adult zebrafish upon MeHgCl exposure. The levels of myo-inositol (MI) in the intestine and brain were decreased and positively correlated. 16 S rRNA sequencing data from adult zebrafish showed that MeHgCl exposure also shifted the structure of gut microbiota and reduced the relative abundance of Bacteroidetes and Proteobacteria , which were further identified at genus level as Aeromonas and Cetobacterium . Further functional analysis indicated that MeHgCl disrupted inositol phosphate metabolism of gut microbiota. Notably, MI supplementation restored the impairment of locomotor activity and inhibited the upregulation of apoptosis and autophagy related genes, such as bcl-2 and atg5 . Thus, this study not only revealed the key role of gut microbiota in MeHgCl-mediated neurotoxicity but also gave new insights into antagonizing its toxicity.
研究领域:
毒理学
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神经科学
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微生物学
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代谢组学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
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2017
·Vol. 134
·期 16
·
IF 1.86
Hui Shi,Xuan Chen,Wenkai Chen,Sujuan Pang,Lisha Pan,Nai Xu,Tan Li
In this work, use of myo -inositol as a biobased nucleating agent (NA) for PLLA was researched. Effects of myo -inositol on non-isothermal and isothermal crystallization behaviors of PLLA at temperatures ranged from 85 °C to 130 °C were studied by using DSC, POM and WAXD. Isothermal crystallization kinetics results showed that the incorporation of myo -inositol enhanced significantly the crystallization rate of the PLLA samples. It was confirmed that the optimum isothermal crystallization temperature range was 100 to 110 °C. The above results were instructive to confirm proper heat treatment time and temperature for compression or injection molding to fabricate highly crystallized PLLA articles. The relations among heat treatment time, crystallinity, heat resistance, and mechanical performances of the neat PLLA and PLLA/1% myo -inositol specimens prepared by compression molding were investigated. Compared with the PLLA specimens, the PLLA/1% myo -inositol specimens showed a shorter heat treatment time to reach the maximum crystallinity. Vicat softening temperature, as well as tensile strength, modulus, and toughness of the PLLA/1% myo -inositol specimens was improved when crystallinity increased from 5.4% to 38.1%. Considering the nontoxicity and biocompatibility of myo -inositol, PLLA/ myo -inositol blends would be potential to prepare some products, which are required higher health standard and can be used in elevated temperature environments. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 44732.
研究领域:
材料科学
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高分子化学
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产品关联
DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2021
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IF 4.401
Pingkang Qian,Kun Gao,Zheng Gao,Chunye Xue,Xiaoli Hu,Qiaoqiao Liu,Jingwei Dong,Xiangyuan Deng
The aim of this work was to investigate the effects of myo -inositol (MI) on growth, photosynthetic activity , and chemical compositions of Chlorella sorokiniana . Results showed that a maximum value of cell density was obtained when this alga was cultivated in BG11 media with 1000 mg L −1 MI, which was 1.37 times greater than that in the controls. Moreover, it was observed that MI had a negative effect on algal photosynthetic activity, which could be alleviated by regulating the contents of chemical compositions, such as chlorophyll a (Chl a ). In the BG11 media with MI, contents of Chl a in the algal cells were in the range of 0.30–0.35 μg 10 −6 cells, which were about 1.75 times greater than that in the controls during microalgae cultivation. The accumulated Chl a would be used to capture and deliver more photons during algal photosynthesis . In addition, contents of lipids , carbohydrates, and proteins varied with the changes in MI concentrations. Thus, it is concluded that MI could promote the algal growth and play an important role in regulating the biochemical compositions in algal cells. These data would provide basic theory and technical support for large-scale cultivation and biomass production of microalgae by adding MI to culture media.
研究领域:
水生生物学
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生物化学
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产品关联
DOI
高影响因子
FLUID PHASE EQUILIBRIA
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2013
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IF 2.379
Yonghong Hu,Wenbiao Zhi,Mengmeng Liang,Zheng Cao,Wenge Yang
In this paper, we focused on solubility and solution thermodynamics of myo -inositol. By gravimetric method, the solubility of myo -inositol was measured in (water + acetic acid) binary solvent mixtures from 278.15 K to 333.15 K under atmosphere pressure. The solubility data were fitted using modified Apelblat equation, a variant of the combined nearly ideal binary solvent/Redich–Kister (CNIBS/R–K) model and Jouyban–Acree model. The three models were all proven to give good agreement with the experiment data, and modified Apelblat equation was the best. By means of van’t Hoff analysis, the enthalpy, entropy and Gibbs energy were calculated, which indicated that the dissolving process was nonspontaneous.
研究领域:
化学
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物理化学
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产品关联
DOI
高影响因子
MACROMOLECULES
·
2021
·
IF 5.985
Xiaodong Xu,Lujuan Li,Seyed Mohsen Seraji,Lei Liu,Zhen Jiang,Zhiguang Xu,Xin Li,Sheng Zhao,Hao Wang,Pingan Song
Spider silk-inspired hydrogen-bond (H-bond) cross-linking has recently been shown to enable polymers, e.g., poly(vinyl alcohol) (PVA), to achieve high strength in combination with large ductility and great toughness by adding small H-bond cross-linkers. Unfortunately, the correlation of H-bond cross-linking to the microstructure and mechanical performances of the resultant polymers remains unclear. Herein, we prepare strong and tough nanostructured PVA composites with inositol (IN) molecules as cross-linkers. The addition of 1.0 wt % of IN increases the yield strength (σy) of PVA up to 148 MPa (by ∼31%), in combination with appreciable increases in the break strain (by 250%) and toughness (by 3.6-fold) because of dynamic physical cross-linking and crystalline grain refinement. We show that there exists a close but simple correlation between the H-bond cross-linking density (ne) and σy, the chain movement (e.g., glass transition temperature (Tg), relaxation activation energy (Ea)) and the crystalline grain size (L), namely, σy ∝ ne, Tg, Ea, and 1/L. This work casts light on the governing effect of H-bonding cross-linking on the microstructure and mechanical properties of PVA and unveils its correlation to mechanical properties, chain dynamics, and crystallization for the first time. These exciting findings open up many new opportunities for creating strong, tough, and ductile polymeric materials.
研究领域:
材料科学
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生物仿生学
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高分子化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2021
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IF 4.821
Zeming Huang,Yin Gao,Zhengyu Huang,Donglin Chen,Jiahui Sun,Li Zhou
Exploring an effective sensor to detect Fe 3+ and phytic acid (PA) is of great importance because the levels of Fe 3+ and PA have a vital impact on human health. Herein, we report a metal-free fluorescent nanoprobe for sensitive and selective sensing of Fe 3+ and PA based on the sulfur quantum dots (SQDs). The SQDs with high fluorescence quantum yield (17.6%) and low cytotoxicity can be directly synthesized from cheap elemental sulfur by one-pot reaction. The SQDs display responsive and selective fluorescence “on–off-on” behavior towards Fe 3+ and PA based on the introduction and elimination of electron transfer effect. The addition of Fe 3+ can cause non-radiative electron transfer associated with fluorescence quenching of SQDs, while the addition of PA enables SQDs/Fe 3+ complex release Fe 3+ to recover the fluorescence of SQD S . By utilizing these SQDs as a fluorescent nanoprobe, it shows good linear relationship for Fe 3+ and PA in the range of 0.05–120 µM and 0–12 µM, respectively. Moreover, its limit of detection (LOD) for Fe 3+ and PA can reach as low as 102 and 73.5 nM, respectively, which compares favorably to many reported fluorescent probes. Additionally, the use of this SQDs-based sensor to detect PA in fresh carrot and lettuce samples was examined with satisfactory performance.
研究领域:
生物化学
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分析化学
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纳米医学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2021
·
IF 7.963
Ruohua Qu,Qiting Xie,Jiang Tian,Min Zhou,Fei Ge
Phosphorus removal by algae-based biotechnology can be achieved through algal assimilation, surface adsorption, or abiotic precipitation. However, there are still unavailable how these phosphorus removal processes were affected by nanoparticles in wastewater. Here, we employed a non-targeted metabolomic approach to reveal the impact of silver nanoparticles (AgNPs) on the phosphorus assimilation by a unicellular green alga Chlorella vulgaris F1068 ( C. vulgaris F1068). Results showed that AgNPs mostly inhibited total phosphorus (TP) removal by the algal assimilation, with TP removal efficiency being reduced by 66.2% (with 0.20 mg/L AgNPs) of the control (without AgNPs). Metabolomics analysis also indicated that AgNPs disturbed metabolic responses related to phosphorus assimilation. AgNPs inhibited phospholipid metabolism which included inositol phosphate metabolism and phosphatidylinositol signaling system (downregulation of glycerol-3-phosphate and myo -inositol, as well as upregulation of serine). Metabolites related to phosphorus assimilation products were impacted through downregulation of guanine , glutamine, alanine , and aspartic acid , as well as upregulation of succinic acid, thereby impeding the algal assimilation of phosphorus. Moreover, perturbation of glutathione metabolism induced by oxidative stress stimulated the alteration of membrane state (upregulation of glycine). These findings contribute to a molecular-scale perspective of nanoparticles on algae-based biotechnology in phosphorus removal.
研究领域:
环境科学
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生物技术
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代谢组学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2022
·Vol. 23
·期 23
·14831
·
IF 6.208
Aiyun Yang,Shen Li,Yan Zhang,Xiuwei Wang,Zhen Guan,Zhiqiang Zhu,Yingchao Liang,Lijiao Zhao,Jianhua Wang
Neural-tube defects (NTDs) are one type of the most serious birth defects. Studies have shown that inositol deficiency is closely related to the occurrence of NTDs. Bone morphogenetic protein (BMP)-mediated Smad signaling pathways have been implicated in neurogenesis and neural-tube closure. However, the role of the BMP/Smad pathway in inositol-deficiency-induced NTDs remains unclear. Inositol-deficiency models in C57 mice and mouse neural stem cells (mNSCs) were induced with Li2CO3treatment or inositol withdrawal. The role of the BMP/Smad pathway in the regulation of cell proliferation and the development of NTDs was determined utilizing qRT-PCR, HE staining, Western blot, immunostaining, MTT assay, EdU staining, and flow cytometry. The intraperitoneal injection of Li2CO3at Embryonic Day 7.5 induced the occurrence of NTDs. The mRNA levels ofBmp2,Bmp4,Smad1,Smad5,Smad8andRunx2, the phosphorylation of Smad1/5/8, and the nuclear translocation of Runx2 were significantly increased in NTD embryonic brain tissues and mNSCs exposed to Li2CO3or an inositol-free medium, which were suppressed by BMP receptor selective inhibitor LDN-193189. The Li2CO3-induced phosphorylation of Smad1/5/8 was inhibited by inositol supplementation. Cell proliferation was significantly promoted by Li2CO3exposure or the absence of inositol in mNSCs, which was reversed by LDN-193189. These results suggest that the activation of the BMP/Smad signaling pathway might play an important role in the development of NTDs induced by maternal Li2CO3exposure via inositol deficiency.Keywords:inositol;BMP signaling;neural-tube defects;neural stem cell;proliferation
研究领域:
发育生物学
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神经科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Zhixin Liu,Xingtao Zuo,Shi Zhang,Juan Xiong,Jiajie He,Yun Zhou,Cong Ma,Zhongbing Chen,Shuili Yu
Developing membranes with highly permeable and selective separation remains a challenge. Here, inositol hexaphosphate (IP6) was first introduced into the active layer for preparing an IP6 integrated polyamide (IP6PA) thin-film composite (TFC) membrane. The IP6PA TFC membrane possessed a thinner PA layer with a smoother and better hydrophilic surface as well as strengthened electronegativity and enlarged pore size. The IP6PA membrane that was prepared with a low IP6 content presented a high pure water flux of 20.5 LMH/bar (1.6 times higher than that of the control membrane), and a high Na 2 SO 4 rejection rate (98.5 %), which could be used to completely remove typical dyes and natural organic matters. Notably, the IP6PA membrane fabricated with a high weight ratio of IP6 exhibited an ultrahigh water flux (111.7 LMH/bar) while maintaining superior rejections for Congo red (98.5 %), Direct red 23 (98.6 %), and Brilliant blue R250 (99.5 %). The results show that the modified membrane presented excellent performance in separation of organic/salts, particularly with its outstanding permeability and superior rejection rate, compared with those of most reported polymeric membranes. This work provides a new scheme for the rational design and manufacture of natural acid regulated polyamide composite membranes with enhanced performance for water treatment.
研究领域:
材料科学
·
环境工程
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DOI
高影响因子
JOURNAL OF CEREAL SCIENCE
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2019
·
IF 2.452
Feier Cheng,Xinhui Ge,Caifeng Gao,Yunlong Li,Min Wang
Epidemiological studies have shown that D-chiro-inositol (DCI) has multiple health effects in humans. Buckwheat , a nutritional staple crop, is a dietary source of DCI. We established a new economic method to accurately detect DCI content in buckwheat samples and investigated the antioxidant effect of DCI. In this study, we analyzed the content and form of DCI in buckwheat with different varieties, growth stages, and milled parts of seeds using pre-column derivatization and high performance liquid chromatography-ultraviolet detection. The total DCI content of ZK-2 and DT-1 was the highest. During buckwheat growth, DCI content was the highest in mature leaves. DCI was mainly in bound form in flowers and grains and mainly in free form in roots and stems. The highest content of DCI was 60–100-mesh in size in buckwheat grains. The results of cell experiments showed that DCI can inhibit the oxidative stress of HepG2 cells induced by palmitic acid (PA). This study will assist in the processing and utilization of buckwheat and provide an important basis for the scientific and effective use of D-chiro-inositol resources to increase the maximization of buckwheat resources.
研究领域:
营养学
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生物化学
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药理学
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农业科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Tao Sun,Zhixiang Li,Shubin Li,Lei Chen,Weiwen Zhang
Cyanobacteria have been considered as an ideal photosynthetic chassis for CO 2 recycle and bioconversion, which could make a significant contribution to carbon neutrality. However, several inherited issues associated with current cyanobacterial strains need to be addressed before their biotechnological application, such as slow growth, uneven of carbon distribution, biased production of reducing power and imbalance between growth and biosynthesis. To address the issues, myo -inositol (MI) was selected as an example target and a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 was utilized as chassis to improve cell growth. Second, several genes involved in MI synthesis were evaluated. Third, the MI biosynthesis was further increased by cofactors enhancement and down regulation of the competing pathways using multiplex artificial small RNAs (PTRNAs). Forth, a MI sensor was developed and optimized to trigger the automatic expression PTRNAs to achieve real-time intelligent regulation, avoiding the growth defect caused by blocking essential genes at early growth stage. Fifth, carbon re-direction was achieved using rhythmical cultivation, resulting in a final production of 262.6 mg/L MI in shaking flask, which represents the highest production from CO 2 in cyanobacteria. Finally, the metabolomic analysis was applied to elucidate the shifted metabolic flux and predicted targets for future optimization. Together of all efforts, we can achieve the CO 2 -based MI biosynthesis to the same order of magnitude as heterogenous microorganisms with suitable scale-up in the future.
研究领域:
生物技术
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微生物学
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代谢工程
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合成生物学
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DOI
高影响因子
Materials
·
2021
·Vol. 14
·期 19
·5481
·
IF 3.623
Songping Mo,Yuanhong Li,Shaofei Shan,Lisi Jia,Ying Chen
Sugar alcohols are phase-change materials with various advantages but may suffer from leakage during applications. In this study, inositol nanocapsules were synthesized at various conditions, including the amount of precursors and the time for adding the precursors. The effects of synthesis conditions on the properties of the nanocapsules were studied. The morphology, chemical composition, microstructure, phase-change characteristics and size distribution of the nanocapsules were investigated by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), differential scanning calorimeter (DSC) and a zeta potential analyzer. The results confirm that inositol was well-encapsulated by an SiO2shell. The shell thickness increased, while the supercooling degree of the nanocapsules decreased with increasing time for adding the precursors. In order to obtain nanocapsules with good morphology and phase-change characteristics, the time for adding the precursors should increase with the amount of precursors. The nanocapsules with the best properties exhibited high melting enthalpy, encapsulation ratio and energy storage efficiency of 216.0 kJ/kg, 83.1% and 82.1%, respectively. The size of the nanocapsules was remarkably affected by the triethoxysilane (TES) amount.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）