Kepeng Hu,Erna Jia,Qimeng Zhang,Wei Zheng,Rongjiao Sun,Mengao Qian,Ying Tan,Wanle Hu
Injectable hydrogel is of interesting for wound healing due to it can be used as carriers of bioactive molecules for the reparation of tissues with minimal invasiveness. However, the integration of lipid-soluble substances into hydrogel network is difficult because of the polarity differences. Here, the tea tree oil (TTO) is encapsulated into the hydrogel network via a previous emulsification process, and a tough and antibacterial injectable hydrogel is synthesized by the Schiff base reaction between carboxymethyl chitosan (CMCS) and genipin (GP). CMCS is served as both an emulsifier and a gel-forming material to construct the heterogeneous hydrogel. The obtained hydrogels present high adhesive strength (∼162.75 kPa), great antibacterial properties (over 90 %) and excellent biocompatibility. Moreover, an anal fistula-like wound healing experiment concluded that the heterogeneous hydrogel has good slow-release properties of TTO for an accelerate healing process, this hydrogel shows great potential for the treatment of complex anal fistula wounds.
An anal fistula caused by a perianal abscess is a hollow tubular structure connecting anal canal with skin and often in irregular shapes. The surgical treatment causes damage to the surrounding tissues and results in complications. Current filling plugs in the clinic, derived from the porcine small intestinal submucosa, only seal the opening and cannot fill into the irregular fistula passage due to the poor liquidity, and fails to control the high-frequent infections around fistulas. Injecting gel-like material is a direct and effective way. To this end, we reported injectable microgels from elastic cryogels of biodegradable disulfide-based crosslinking. Microgels with shear-thinning and self-healing remain solid under low strain and fluid under high strain to ensure successful filling and repair the multiple-branched irregular canals. Nano-silver dopped microgels presented antibacterial efficacy . In rabbit models of anal and muscle fistula, the inflammatory cells significantly decreased. CD31 and α-smooth muscle actin (α-SMA) showed the fistula healing was improved considerably. These findings demonstrated that self-healable microgels have enormous potential to repair the abnormal and complicated tubular passages caused by ulcers and fistulas.
危险品化学品经营许可证（带存储）