Liver disease is a great danger to human health. The determination of blood level of Cholyglycine acid (CG) is a vital biomarker for the assessment of liver function in clinic, which is contribute to the diagnosis of liver diseases. Thus, establishing accuracy, rapid and convenient
method for the detection of glycolic acid is of great significance. In this study, a time-resolved-fluorescence (TRF) lateral flow assay for rapid detection of CG was development. The analytical detection limit (mean of zero-2 SD) was 0.06 μg/mL The method showed good linearity in
the range of 0.2–40 g/mL and was not affected by biomolecules with similar structure to CG. The analytical mean recovery of control was between 90–110% and the imprecision of intra- and inter-assay of CVs was less than 10%. No significant matrix effect was observed in saline, serum,
plasma or whole blood. A good correlation was found with the homogeneous enzyme immunoassay (HEIA) assay (slopes 1.0463, y-intercepts 0.2721 μg/mL, R = 0.989, n = 50, P < 0.001). The CG TRF analysis could provide reproducible and quantitative information
about the state of liver in a few minutes, which is suitable for the detection of liver diseases in point-of-care-testing (POCT) conditions.
Jianwei Wang,Yeshuai Song,Xinyu Dou,Jiapeng Sun,Xinghua Yang,Yu Zhang,Zhihai Liu,Yanzeng Li,Hanyang Li
Real-time monitoring of liver injury is essential for preserving physiological health. Alanine aminotransferase (ALT) detection is widely regarded as a fundamental approach for the early diagnosis of liver injury. However, existing detection methods often suffer from complex operation, high costs, and limited sensitivity. Here, we introduce a real-time biosensor based on functionalized liquid crystal microcavities with whispering gallery mode (WGM) laser for rapid and sensitive ALT detection. The microcavity functionalized with stearic acid exhibits distinct optical responses to ALT-catalyzed enzymatic reactions across varying concentrations. The high-quality factor of the microcavity obviously enhances its biosensing performance. Simulations reveal variations in the electric field behavior of WGM lasing as the liquid crystal microcavity transitions between radial and bipolar configurations. Further analysis was conducted using experimental WGM spectra and corresponding polarized optical microscopy images. Experimental results demonstrate a strong linear correlation between ALT concentration and reaction time within the range of 0 to 240 U/l. This biosensor exhibits a sensitivity of 0.67 s/(U/l), indicating its potential as a promising approach for early liver injury assays. In addition to in vitro verification, in vivo validation using mouse serum samples further confirms its practical applicability, yielding results consistent with those tested by using commercial assay kits. This method offers a simple, cost-effective, and efficient detection of ALT, underscoring the potential of liquid crystal microcavities for biosensing in liver injury monitoring.
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