PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2016
·Vol. 33
·期 7
·388-395
·
IF 4.367
Wenbin Wang,Liqiang Liu,Liguang Xu,Hua Kuang,Jianping Zhu,Chuanlai Xu
Staphylococcal enterotoxins (SEs) in food samples can cause severe gastroenteritis even at low doses. Detection of SEs with a single assay would not only improve reliability and portability, but would also save time. In this paper, for the first time, a multiplexed gold-nanoparticle-based immunochromatographic (ICT) strip that allows the simultaneous detection of the five main SEs has been reported: SEA, SEB, SEC, SED, and SEE. Based on the sandwich immunoassay of each SE, a multiplexed ICT strip with five test lines is developed. Sensitive and specific paired monoclonal antibodies facilitate the detection, allowing for high sensitivity and good specificity. The portable biosensor platform can simultaneously detect SEA, SEB, SEC, SED, and SEE at concentrations as low as 2.5, 2.5, 2.5, 1, and 5 ng mL −1 , respectively. The entire test can be performed within 15 min and requires no sophisticated instruments except a portable strip reader. To evaluate the multiplexed strip, naturally SEA and SEC contaminated milk samples and pure milk samples spiked with the five SEs are analyzed.
研究领域:
食品安全检测
·
免疫学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Weipeng Tong,Yingjie Du,Mingjian Yao,Hao Fang,Weitao He,Yi Zhang,Yu Su,Yuankui Leng,Xiaolin Huang,Ying Xiong,Yonghua Xiong
An innovative light scattering immunoassay was developed using an AuNPs etching strategy. Three types of anisotropic gold nanoparticles, including gold nanocubes, nanorods, and nanoflowers with distinct morphologies, were utilized to investigate how these morphological differences affect the sensitivity of light scattering signal transduction. Based on theoretical insights into light scattering and electromagnetic fields, gold nanocubes were identified as the optimal probes for enhancing light scattering signal transduction and were employed to construct an immunoassay for detecting staphylococcal enterotoxin A (SEA). The developed immunoassay achieved ultrahigh sensitivity for SEA detection in milk samples, with a detection limit of 10.39 pg mL −1 , which is 190 times lower than that of conventional ELISA. The proposed immunoassay was validated across ten food samples, demonstrating high accuracy and robustness. Given these promising results, we believe this method has significant potential for screening trace levels of SEA in food products.
研究领域:
生物医学工程
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Hui Li,Yan-yan Huang,Keren Agyekumwaa Addo,Yi-gang Yu,Xing-long Xiao
The inhibitory mechanism of CUM on Staphylococcus aureus exotoxins and invasive enzyme, and the effect of CUM on sauced beef were investigated. Treatment with sub-minimum inhibitory concentrations of CUM observed very little decrease in cell density after 24 h. Additionally, CUM reduced hemolytic activity by inhibiting the formation of heptamer, and inhibited the content of enterotoxin in culture medium and sauced beef. The transcription of genes encoding α-hemolysin, staphylococcal enterotoxin A and Panton-Valentine leukocidin were down-regulated after treatment with CUM. Compared with the control group, the addition of CUM caused a significant decrease in S. aureus counts, total volatile base nitrogen, acid value, and thiobarbituric acid reactive substances of sauced beef after 9 d. Furthermore, CUM improved the redness (a*) of sauced beef. These results confirmed that CUM could effectively restrain the synthesis of S. aureus toxin, and the addition of CUM to sauced beef was the potential way to inhibit the growth of food pathogens and maintain quality profiles.
研究领域:
食品科学
·
微生物学
·
毒理学
·
食品安全
✓ 已验证
DOI
高影响因子
Chemosensors
·
2023
·Vol. 11
·期 8
·453
·
IF 4.2
Kai Mao,Lili Tian,Yujie Luo,Qian Li,Xi Chen,Lei Zhan,Yuanfang Li,Chengzhi Huang,Shujun Zhen
Enzyme-linked immunosorbent assay (ELISA) is one of the most commonly used method for the detection of staphylococcal enterotoxin B (SEB), the main protein toxin causing staphylococcal food poisoning. However, the traditional ELISA reaction needs to be stopped by sulfuric acid to obtain stable colorimetric signal, and it is easily influenced by a colored sample. In order to address this problem, a new ELISA method using zeolite imidazolate skeleton-8 metal-organic framework (ZIF-8 MOF) as a light scattering (LS) reporter for SEB detection was developed in this work. ZIF-8 MOF has the characteristics of high porosity, large specific surface area, clear pore structure, and adjustable size, which is one of the most representative MOFs constructed from Zn2+and 2-methylimidazole (2-mIM). The 2-mIM ligand of ZIF-8 exhibited antioxidant activity and can strongly react with H2O2, which could destroy the structure of ZIF-8, resulting in the obvious decrease in LS intensity. We combined this specific reaction with the sandwich immune reaction to construct the LS ELISA method for the successful detection of SEB. This method is more reliable than commercial tests kits for the detection of colored samples, and it is simple, sensitive, and selective, and has great potential in the detection of other toxins by simply changing the corresponding recognition units.Keywords:ELISA;light scattering;metal organic framework;ZIF-8;staphylococcal enterotoxin B
研究领域:
生物化学
·
食品安全检测
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
Pharmaceutics
·
2023
·Vol. 15
·期 5
·1493
·
IF 5.4
Chaojun Song,Yutao Liu,Jinwei Hu,Yupu Zhu,Zhengjun Ma,Jiayue Xi,Minxuan Cui,Leiqi Ren,Li Fan
Highly sensitive staphylococcal enterotoxin B (SEB) assay is of great importance for the prevention of toxic diseases caused by SEB. In this study, we present a gold nanoparticle (AuNP)-linked immunosorbent assay (ALISA) for detecting SEB in a sandwich format using a pair of SEB specific monoclonal antibodies (mAbs) performed in microplates. First, the detection mAb was labeled with AuNPs of different particle sizes (15, 40 and 60 nm). Then the sandwich immunosorbent assay for SEB detection was performed routinely in a microplate except for using AuNPs-labeled detection mAb. Next, the AuNPs adsorbed on the microplate were dissolved with aqua regia and the content of gold atoms was determined by graphite furnace atomic absorption spectrometry (GFAAS). Finally, a standard curve was drawn of the gold atomic content against the corresponding SEB concentration. The detection time of ALISA was about 2.5 h. AuNPs at 60 nm showed the highest sensitivity with an actual measured limit of detection (LOD) of 0.125 pg/mL and a dynamic range of 0.125–32 pg/mL. AuNPs at 40 nm had an actual measured LOD of 0.5 pg/mL and a dynamic range of 0.5 to 128 pg/mL. AuNPs at 15 nm had an actual measured LOD of 5 pg/mL, with a dynamic range of 5–1280 pg/mL. With detection mAb labeled with AuNPs at 60 nm, ALISA’s intra- and interassay coefficient variations (CV) at three concentrations (2, 8, and 20 pg/mL) were all lower than 12% and the average recovery level was ranged from 92.7% to 95.0%, indicating a high precision and accuracy of the ALISA method. Moreover, the ALISA method could be successfully applied to the detection of various food, environmental, and biological samples. Therefore, the successful establishment of the ALISA method for SEB detection might provide a powerful tool for food hygiene supervision, environmental management, and anti-terrorism procedures and this method might achieve detection and high-throughput analysis automatically in the near future, even though GFAAS testing remains costly at present.Keywords:gold nanoparticles;gold nanoparticle-linked immunosorbent assay;staphylococcal enterotoxin B
研究领域:
生物化学
·
医学检测
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Environmental Research and Public Health
·
2013
·Vol. 10
·期 4
·1598-1608
·
IF 1.998
Hua Kuang,Wenbing Wang,Liguang Xu,Wei Ma,Liqiang Liu,Libing Wang,Chuanlai Xu
A sensitive and specific monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (ELISA) was established and validated for the detection of staphylococcal enterotoxin A (SEA). After routine fusion and selection, 10 monoclonal antibodies showed high affinity for SEA. An optimal pair for sandwich ELISA was selected by pairwise interaction analysis. After optimization, the limit of detection (LOD) and linear dynamic range of the method were established, and were found to be 0.0282 ng/mL and 0.06–2 ng/mL, respectively. The recovery in pure milk ranged from 82.67% to 111.95% and the intra- and inter-assay coefficients of variation ranged from 3.16% to 6.05% and from 5.16% to 10.79%, respectively. Cross-reactivity with staphylococcal enterotoxin B (SEB), staphylococcal enterotoxin C (SEC), staphylococcal enterotoxin D (SED), and staphylococcal enterotoxin E (SEE) in this method were insignificant. These results indicate that the sandwich ELISA method developed in our study is effective for routine identification of SEA in food samples.
研究领域:
食品科学
·
免疫学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Yijie Wang,Yue Huang,Yan Zhang,Henan Wang
Staphylococcal enterotoxins as common bacterial toxins can bring about food contamination and lead to severe foodborne illnesses, so facile and sensitive detection of staphylococcal enterotoxins is significant to ensure food safety and protect public health. In this work, an ultrasensitive biosensor for staphylococcal enterotoxin B (SEB) detection is developed based on the novel electrochemical nanoprobe named MXene/AgNPs@pSC4/MB, which is prepared by functionalizing MXene with para-sulfonatocalix[4]arene-decorated silver nanoparticles (AgNPs@pSC4) and electroactive signal molecules methylene blue (MB). The collaborative effect of AgNPs and MXene endows the fabricated electrochemical nanoprobe with excellent conductivity and admirable loading capacity of signal molecules to significantly enhance the response of biosensor, while pSC4 as the robust linker can bridge the nanoprobe and target SEB by binding with multifarious amino acid residues on SEB. With the specific aptamer immobilized on the electrode as the identifying element to capture SEB, the electrochemical nanoprobes MXene/AgNPs@pSC4/MB are anchored to SEB via pSC4, producing remarkably amplified signal output. The proposed electrochemical biosensor facilitates the ultrasensitive detection of SEB with a low detection limit of 1.76 × 10 −7 ng/mL in addition to the merits of facile operation and rapid response. Moreover, benefiting from the high chemical stability and good resistance to fouling and passivation of the electrochemical nanoprobe, the biosensor exhibits long-lasting stability, good accuracy and anti-interference ability in SEB detection. The reliable detectability of the biosensor has also been examined in the analysis of real food samples, demonstrating potential application toward food safety monitoring.
研究领域:
食品安全检测
·
生物传感
·
纳米技术
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
TOXICON
·
2022
·
IF 3.035
Mao Zhang,Hui Li,Addo Keren Agyekumwaa,Yigang Yu,Xinglong Xiao
Staphylococcus aureus ( S. aureus ) is known to be one of the most common foodborne pathogens capable of secreting a wide range of exotoxins such as enterotoxin , which severely threatens the health of consumers. Over the past few years, the development of safe and effective strategies in inhibiting the growth and enterotoxins generation of S. aureus in food turns out to be the research focus and emphasis. This research explores citronellal (CIT), a native compound with extensive existence in spices, which could effectively inhibit the growth and enterotoxins generation of S. aureus (ATCC 29213). Results from minimal inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and time-kill curves, showed that CIT could tremendously inhibit the growth of S. aureus . Analysis on hemolysin showed that CIT at sub-MIC could significantly (p < 0.05) inhibit the hemolytic activity of S. aureus . As revealed by the results of ELISA , the production of enterotoxins in the culture supernatant and pork meat decreased significantly (p < 0.05) after exposure to CIT at sub-MIC. Furthermore, a significant (p < 0.05) decrease in dose-dependent was found in the transcription levels of virulence-related genes. In all, CIT proved to be a possible inhibitor of the growth and enterotoxins production of S. aureus with highly promising application in the food industry.
研究领域:
食品科学
·
微生物学
·
毒理学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2015
·
IF 2.587
Xiaoqian Chen,Qingxiang Wang,Liheng Wang,Hongxu Guo,Yizhen Yang,Feiming Li,Feng Gao
Rugby-ball-shaped CoS2 (rCoS2) submicroparticles have been synthesized via a simple hydrothermal reaction. The rCoS2 submicroparticles were then casted on a glassy carbon electrode and served as a highly conductive and large surface area platform for the electrosynthesis of poly(2-thiophenesulfonyl chloride) (pTSC), using 2-thiophenesulfonyl chloride as the monomer. The stepwise modification process was characterized by attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR) and different electrochemical technologies. The stable pTSC layer was utilized as a functional interface to immobilize amino-modified probe DNA via a facile sulfonyl–amino condensation reaction, without the assistance of any other cross-linkers. Because of the synergistic effect of rCoS2 and pTSC from the hybrid material, the developed DNA biosensor could detect target DNA over a wide dynamic range, from 1.0 × 10–13 to 1.0 × 10–7 M, with the detection limit down to 1.1 × 10–14 M. Benefiting from the steady electropolymerization film of pTSC and the stable sulfamide bond between the amino-modified DNA and pTSC, the biosensor also showed high stability, reusability, and reproducibility.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Yukun Huang,Hui Zhang,Xiujuan Chen,Xiaole Wang,Nuo Duan,Shijia Wu,Baocai Xu,Zhouping Wang
Food safety is one of the most important public health issues worldwide. Foodborne illnesses caused by Staphylococcus aureus enterotoxins (SEs) commonly occur, affecting both developing and developed countries. In this study, multicolor lanthanide-doped time-resolved fluorescence nanoparticles labeled with aptamers were used as bioprobes, and graphene oxide (GO) was employed as a resonance energy acceptor. Based on the “turn down” strategy, the simultaneous detection of multiplex SEs was realized in a homogeneous solution. Under the optimal conditions, the developed method exhibited high sensitivity and selectivity to three serological types of enterotoxins, including type A, B, C1, with limits of detection below 1 ng mL −1 . The application of this bioassay in milk analysis with no sample dilution was also investigated, and the results of recovery rates covered from 92.76% to 114.58%, revealing that the developed method was accurate. Therefore, this detection aptasnesor can be a good candidate for multiplex analysis and screening with simple and effective operations.
研究领域:
食品安全
·
生物传感技术
·
纳米材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbiology Spectrum
·
2022
·
IF 9.043
Zhaoxi Deng,Kangwei Hou,Teresa G. Valencak,Xin M. Luo,Jianxin Liu,Haifeng Wang
The LuxS enzyme plays a key role in both quorum sensing (QS) and the regulation of bacterial growth. It catalyzes the production of autoinducer-2 (AI-2) signaling molecule, which is a component of the methyl cycle and methionine metabolism. This study aimed at investigating the differences between the Lactobacillus rhamnosus GG (LGG) wild-type strain (WT) and its luxS mutant (ΔluxS) during biofilm formation and when resisting to inflammation caused by Enterotoxigenic Escherichia coli (ETEC) in germ-free zebrafish. Our results suggest that in the absence of luxS when LGG was knocked out, biofilm formation, extracellular polysaccharide secretion and adhesion were all compromised. Addition of synthetic AI-2 indeed rescued, at least partially, the deficiencies observed in the mutant strain. The colonizing and immunomodulatory function in WT versus ΔluxS mutants were further studied in a germ-free zebrafish model. The concentration of AI-2 signaling molecules decreased sharply in zebrafish infected with the ΔluxS. At the same time, compared with the ΔluxS, the wild-type strain could colonize the germ-free zebrafish more effectively. Our transcriptome results suggest that genes involved in immunity, signal transduction, and cell adhesion were downregulated in zebrafish infected with ΔluxS and WT. In the WT, the immune system of germ-free zebrafish was activated more effectively through the MAPK and NF-κB pathway, and its ability to fight the infection against ETEC was increased. Together, our results demonstrate that the AI-2/LuxS system plays an important role in biofilm formation to improve LGG and alleviate inflammation caused by ETEC in germ-free zebrafish.IMPORTANCE Lactobacillus rhamnosus GG is a widely used probiotic to improve host intestinal health, promote growth, reduce diarrhea, and modulate immunity. In recent years, the bacterial quorum sensing system has attracted much attention; however, there has not been much research on the effect of the LuxS/AI-2 quorum sensing system of Lactobacillus on bacteriostasis, microbial ecology balance, and immune regulation in intestine. In this study, we used germ-free zebrafish as an animal model to compare the differences between wild-type and luxS mutant strains. We showed how AI-2/LuxS QS affects the release of AI-2 and how QS regulates the colonization, EPS synthesis and biofilm formation of LGG. This study provides an idea for the targeted regulation of animal intestinal health with probiotics by controlling bacteria quorum sensing system.
研究领域:
微生物学
·
免疫学
·
分子生物学
·
动物模型研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2025
·Vol. 14
·期 8
·914
·
IF 6.6
Pengxin Jiang,Kangping Liu,Yanan Cui,Puyu Liu,Xutao Wang,Zijuan Hou,Jiamei Cui,Ning Chen,Jinghui Fan,Jianguo Li,Yuzhu Zuo,Yan Li
EnterotoxigenicEscherichia coli(ETEC) leads to severe diarrhea in piglets. Naringenin (Nar), a natural flavonoid compound, is known for its antibacterial and anti-antioxidant properties. However, the protective effects of Nar against ETEC-induced diarrhea have not been reported yet. This study investigated the protective mechanisms of Nar against ETEC infection in porcine intestinal epithelial cells (IPEC-J2). ETEC infection induced oxidative stress and ferroptosis in IPEC-J2 cells by elevating intracellular iron content and ROS accumulation, increasing MDA levels, downregulating SOD activity and GPX4 expression, and upregulating the transcription of CHAC1 and SLC7A11. In contrast, Nar suppressed ETEC-induced ferroptosis of IPEC-J2 cells by inhibiting the SLC7A11/GPX4 pathway. Specifically, Nar mitigated mitochondrial damage, reduced intracellular iron levels and ROS accumulation, and ultimately reversed the oxidative stress. Network pharmacology and molecular docking identified heat-shock protein 90 (HSP90) as a potential target of Nar. Overexpression and knockdown experiments revealed that ETEC-induced ferroptosis was mediated by upregulation of HSP90, while the protective effects of Nar against ETEC-induced ferroptosis were dependent on the downregulation of HSP90. In conclusion, Nar targets host HSP90 to protect IPEC-J2 cells from ferroptosis caused by ETEC infection. This study demonstrates that Nar is a potent antioxidant natural compound with potential for preventing ETEC-induced intestinal damage.
研究领域:
兽医学
·
微生物学
·
药理学
·
细胞生物学
·
分子生物学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）