JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Buqing Wang,Yasen Wang,Xiaoman Zhou,Xiao-Dong Gao,Morihisa Fujita,Zijie Li
BACKGROUND Acidic lipases with high catalytic activities under acidic conditions have important application values in the food, feed and pharmaceutical industries. However, the availability of acidic lipases is still the main obstacle to their industrial applications. Although a novel acidic lipase Rasamsonia emersonii (LIPR) was heterologously expressed in Escherichia coli , the expression level was unsatisfactory. RESULTS To achieve the high-efficiency expression and secretion of LIPR in Pichia pastoris GS115, the combinatorial optimization strategy was adopted including gene codon preference, signal peptide, molecular chaperone co-expression and disruption of vacuolar sorting receptor VPS10 . The activity of the combinatorial optimization engineered strain in a shake flask reached 1480 U mL −1 , which was 8.13 times greater than the P. pastoris GS115 parental strain. After high-density fermentation in a 5-L bioreactor, the highest enzyme activity reached as high as 11 820 U mL −1 . LIPR showed the highest activity at 40 °C and pH 4.0 in the presence of Ca 2+ ion. LIPR exhibited strong tolerance to methanol, indicating its potential application in biodiesel biosynthesis. Moreover, the gastrointestinal digestion simulation results demonstrated that LIPR was tolerant to pepsin and trypsin, but its activity was inhibited by sodium taurodeoxycholate. CONCLUSION This study provided an effective approach for the high expression of acidic lipase LIPR. LIPR was more appropriate for lipid digestion in the stomach than in intestine according to the gastrointestinal digestion simulation results. © 2024 Society of Chemical Industry.
研究领域:
生物技术
·
酶工程
·
食品科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Jia-Yi Yin,Ya-Ning Han,Meng-Qi Liu,Zan-Hao Piao,Xu Zhang,Yu-Ting Xue,Ying-Hua Zhang
Human health can be damaged by free radicals, and antioxidant peptides are excellent radical scavengers. Antioxidant tripeptides data set based on 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulofnic acid) (ABTS) assay was created, 9 types of descriptors were integrated and 4 quantitative structure–activity relationship (QSAR) models were constructed in this study. Several structural factors influencing the activity of antioxidant tripeptides and the dominant amino acids at each position of tripeptides were revealed by the optimal model. Ten food-derived tripeptides with higher activity were selected for synthesis and activity determination. Molecular docking results demonstrated that these tripeptides were stably bound to the Keap1 receptor, further elucidating the antioxidant mechanism. It was known from the simulation of gastrointestinal digestion experiments that the model results possessed a guiding effect on the selection of proteins with high antioxidant activity . The performance of the model was proved to be robust after validation.
研究领域:
生物信息学
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营养学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Chao Du,Zhenzhen Hui,Na Li,Kaiyue Liu,Yong Wang,Ping Wang,Shuyang Sun
This investigation sought to identify antioxidative peptides derived from Meretrix meretrix L. (MML) throughout simulated gastrointestinal digestion (SGD) via in vitro and in silico strategies. The study demonstrated that hydrolysates from MML captured at various SGD phases showcased robust antioxidative activity. These hydrolysates also displayed a protective role against hydrogen peroxide-induced stress in Raw 264.7 cells. Notably, hydrolysates sourced from the intestinal phase of digestion (MMLHs-I) markedly inhibited the reduction of cell viability, limited the generation of reactive oxygen species under oxidative stress conditions, and promoted the activity of key antioxidant enzymes including superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, thus amplifying the cells' antioxidative defense system. Through nLC-MS/MS analysis, 105 peptides tracing back to 18 parent proteins were pinpointed in MMLHs-I, with 18 of these peptides previously reported in the BIOPEP-UWM database. Subsequent in silico analysis, alongside empirical antioxidant activity assays, pinpointed the peptide WLV as an exceptional antioxidative agent, demonstrating an IC 50 of 0.125 mg/mL in the ABTS assay and an impressive 15.18 μmol Trolox/mg in the ORAC assay. The outcomes highlight MML's promise as a novel source of antioxidative peptides, positioning peptide WLV as a potentially valuable addition to functional foods designed to combat oxidative stress.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Quan Qian,Jun Liang,Zhefan Ren,Jingke Sima,Xiaoyun Xu,Jörg Rinklebe,Xinde Cao
The simulated in-vitro gastrointestinal method provides a simple way to evaluate the health risk of human body exposed to soil contaminants. Several in-vitro methods have been successfully established for soil As, Pb, and Cd. However, the method development for soil Cr failed up to now, which could be resulted from alteration in the species of Cr (e.g., Cr(VI)/Cr(III)) caused by the gastrointestinal digestion components, ultimately affecting the accessibility of Cr. This study explored the transformation and bioaccessibility of Cr in two Cr-contaminated soils during the physiologically based extraction test. The water-soluble and exchangeable Cr in soil was dissolved in gastrointestinal tract, accompanied with reduction of Cr(VI) into Cr(III), and the reduction occurred after the chemical extraction in two soils rather than during the extraction. Pepsin and organic acids in gastric phase could reduce Cr(VI) into Cr(III) and reduction efficiency were 20.4%− 53.0%, while in intestinal phase, pancreatin and bile salt had little effect on the Cr(VI) reduction, instead, more Cr(VI) was released from soil. In the gastric solution, Cr(VI) was mainly present as HCrO 4 - and Cr(III) as free Cr 3+ ion. In the intestinal phase, Cr(VI) mainly occurred as CrO 4 2- and Cr(III) as Cr(OH) 3 (aq). Cr in the soil solid phase was dominated as the precipitates of Cr-Fe oxide, which was hardly extracted. Bioaccessibility of Cr in gastric phase increased as extraction duration increased and decreased in the intestinal phase, the contrary trend was observed for the hazard quotient of Cr in two phases due to Cr(VI)/Cr(III) transformation. This study indicates that the gastrointestinal components could influence the Cr transformation and subsequently affect the Cr bioaccessibility, which would help for a successful establishment of in vitro determination method for soil Cr bioaccessibility.
研究领域:
环境科学
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毒理学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2023
·Vol. 14
·期 20
·9324-9336
·
IF 6.1
Hui Yang,Yingying Hou,Yujie Pan,Tingting Zhang,Qi Meng,Jianzhong Han,Weilin Liu,Daofeng Qu
Chewing ability has a strong effect on food digestion. However, little is known about the relationship between the food mastication degree and the subsequent gastric emptying. This study was to explore the effects of individual chewing ability (strong and weak) on the in vivo oral processing characteristics and in vitro dynamic gastrointestinal starch hydrolysis of three types of rice (japonica rice, indica rice and waxy rice). Results showed that the swallowable bolus in the weak chewing group had larger holes and a looser microstructure with more small rice particles, while the strong chewing ones obtained a bolus with higher saliva content (up to 28%) and starch hydrolysis degree (up to 13.55%). Moreover, the gastric retention and starch hydrolysis of the strong chewing ability group were higher in the artificial gastric dynamic system (AGDS). The indica rice particles with the higher degree of fragmentation contacted enzymes easier and hydrolyzed quicker, thus emptying through the stomach faster (81.76%). However, the oral chewing properties of rice mainly influenced the starch digestion in the stomach and the initial stage of the small intestine (∼5 min). This study suggested that the chewing ability and rice variety can influence the bolus properties, which in turn affected the gastric emptying and the degree of starch hydrolysis during digestion.
研究领域:
食品科学
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消化生理学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2020
·Vol. 11
·期 4
·3483-3492
·
IF 4.171
Zhong-Yuan Liu,Yuan-Yuan Hu,Man-Tong Zhao,Hong-Kai Xie,Xiao-Pei Hu,Xiao-Chi Ma,Jiang-Hua Zhang,Yan-Hong Bai,Da-Yong Zhou
The formation and disappearance of aldehydes during simulated gastrointestinal digestion (SD) of fried clams was investigated in order to shed light on the underlying mechanism. Results from the thiobarbituric acid reactive substance (TBARS) and fluorometric assays using a specific aldehyde probe indicated that the SD (with lipase) of fried clams initially reduced (at the gastric stage), but subsequently increased (mainly at the intestinal stage) the contents of total aldehydes. Meanwhile, eight specific aldehydes including propanal, acrolein, trans-2-pentenal, hexanal, trans,trans-2,4-octadienal, trans,trans-2,4-decadienal, 4-hydroxy-hexenal and 4-hydroxy-nonenal in the digested meal were determined by using a high-performance liquid chromatography-tandem electrospray ionization mass spectrometry (HPLC-ESI-MS/MS) method. Results indicated that the changes in the trend of the contents of the eight aforementioned aldehydes were similar to those of total aldehydes during SD (with lipase) of fried clams. However, a similar SD process without lipase time-dependently reduced the contents of total and individual aldehydes. Moreover, lipid classes and free fatty acids (FFAs) in the digested meal were determined to reveal the degree of hydrolysis of lipids during the SD process. Results indicated that the SD (with lipase) of fried clams significantly hydrolyzed triacylglycerols (TAG) and polar lipids (PL) and produced FFAs, but the SD process without lipase resulted in negligible lipid hydrolysis. Thus, our results demonstrated a positive correlation between lipid hydrolysis and aldehyde generation during the SD of fried clams. Alternatively, unsaturated FFAs instead of TAG and PL could have served as the main precursors for aldehyde generation due to their high oxidative susceptibility.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESS ENGINEERING
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2020
·Vol. 43
·期 12
·e13556
·
IF 1.703
Shenghua He,Sanjiu Zhou,Weiyun Guo,Yonghui Wang,Chunhong Liu,Rongchun Wang,Fugang Xiao
In this study, rapeseed oil bodies (RPOBs) were used to prepare curcumin emulsions (CEs) in order to investigate their stability and gastrointestinal. The results showed that particle size significantly reduced ( p < .05) with increasing RPOBs concentrations from 1.0 to 6.0 wt%. Additionally, CEs showed good stability at pH 2.0–10.0. However, CEs were instable at salt concentration ranging from 100 to 500 mM and at temperatures between 35 and 95°C. CE fat droplet size after 30 min of digestion time exhibited a remarkable increase ( p < .05) compared with other times. Upon exposure to simulated intestinal fluid (SIF) conditions, CEs particle sizes gradually decreased with increasing digestion time. Then, 56% curcumin and 40 μmol/ml free fatty acids were produced from CEs after 180 min digestion time under SIF conditions. Practical applications Our findings indicate that rapeseed oil bodies as a novel carrier can be applied for the delivery-controlled release of lipophilic bioactive compounds from emulsions during gastrointestinal digestion.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 61
·38243-38250
·
IF 3.108
Huimin Feng,Chao Li,Chin Ping Tan,Xiong Fu,Bin Zhang,Qiang Huang
Lutein is a natural colorant and functional ingredient with many health-promoting bioactivities. However, its use in functional foods is limited due to its low water-solubility and chemical instability. The objectives of this study were to investigate the physicochemical properties and in vitro bioaccessibilities of lutein loaded emulsions stabilized by corn fiber gums (CFG1 and CFG2). The physicochemical properties and stabilities of the emulsions were evaluated by droplet size and distribution, ζ-potential, viscosity, visual observation of phase separation, and lutein retention during storage. The lutein bioaccessibilities of the emulsions were measured after digestion in a simulated gastrointestinal tract (SGT). The results showed that CFG2 had better emulsifying capacity and stability than CFG1. Compared to lutein dispersed in corn oil, the chemical stability and in vitro bioaccessibility of lutein in the emulsions were significantly increased through emulsification. These results suggest that corn fiber gum is a promising natural emulsifier, which has potential to fabricate emulsions for delivering lutein or other lipophilic bioactive compounds in functional foods.
研究领域:
食品科学
·
营养学
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Xianjiang Ye,Ran Yang,Zhizhi Yang,Baote Huang,Talha Riaz,Chao Zhao,Jicheng Chen
Food-derived peptides with angiotensin-I-converting enzyme inhibitory (ACEI) activity have garnered significant research attention. This study focused on examining ACEI peptides from Porphyra haitanensis and investigating their potential inhibitory mechanisms through a combination of in silico and in vitro methods. Five novel potential ACEI peptides (ASF: Ala-Ser-Phe, EIIL: Glu-Ile-Ile-Leu, LCVPR: Leu-Cys-Val-Pro-Arg, LDVVN: Leu-Asp-Val-Val-Asn, and LSPSW: Leu-Ser-Pro-Ser-Trp) were identified through a combination of peptidomics and in silico bioinformatics analysis, of which LSPSW showed the highest activity (IC 50 : 5.27 ± 0.53 μM). After simulated gastrointestinal digestion (SGD), the activities of LCVPR and LSPSW remained almost unchanged. The results from inhibition kinetics and molecular docking demonstrated that ACEI peptides with differing inhibition mechanisms could successfully bind to ACE through hydrogen bonds. Based on network pharmacology analysis and cellular experiments, LSPSW exerted antihypertensive effects by regulating the expression levels of peroxisome proliferator activated receptor gamma (PPARG), prostaglandin-endoperoxide synthase 2 (PTGS2), and renin (REN). This research provided a novel theoretical foundation for the high-value application of P. haitanensis .
研究领域:
食品科学
·
生物信息学
·
药理学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Naiyi Yin,Zeliang Han,Huili Du,Pengfei Wang,Yunpeng Li,Xiaochen Chen,Guoxin Sun,Yanshan Cui,Zhengyi Hu
To determine the effect of vitamin supplements on the oral bioaccessibility of Pb in soils, Pb bioaccessibility was measured in the presence of 9 vitamins by a physiologically based extraction test. Gastric Pb bioaccessibility (G-BA, 2.6–83.3%) was found to be mostly reduced (1.1–3.1 fold) in the presence of B vitamins, specifically vitamins B1, B6, and B9. In contrast, a significant increase in Pb G-BA was observed with vitamin C and E involved. In the small intestinal phases, Pb bioaccessibility (I-BA) ranged from 0.1% to 16.0%, being 5–50 fold lower than the corresponding G-BA values. Vitamin C supplementation showed a 7-fold increase in Pb I-BA, with a similar increase presented in approximately 30% of samples treated to vitamin B involvement. Lead liberation in gastrointestinal digests was associated with the dissolution of Fe and Mn regulated by vitamins. In conclusion, the addition of B vitamins resulted in the reduction of gastric Pb bioaccessibility, but the bioaccessibility value increased in participation of vitamin C and E. Elevated intestinal bioaccessibility was found especially for vitamin C. This should contribute to more accurate assessment of health risks from contaminated soils. Nutritional management aimed at preventing Pb-induced toxicity can benefit from knowledge of vitamin influence on soil Pb bioaccessibility.
研究领域:
环境科学
·
营养学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2017
·
IF 3.086
Tao Bao,Yuting Li,Jiahong Xie,Chongde Sun,Xian Li,Yunfeng Pu,Wei Chen
This study was aimed to investigate the impact of in vitro gastrointestinal digestion on some common and new bayberry cultivars. The contents of total phenolics (246–669 mg gallic acid equivalents/kg FW (fresh weight)), flavonoids (116–689 mg quercetin- 3-O -rutinoside equivalents/kg FW), procyanidins (28–133 mg catechin equivalents/kg FW) and anthocyanins (1–7 mg cyaniding- 3-O -glucoside equivalents/kg FW) were detected in digested cultivars. HPLC-TOF-MS analysis identified 17 phenolic compounds in digested sample. Among all digested cultivars, the new cultivars Anhaizaomei (ABTS, IC 50 = 2.95 mg/mL; FRAP, 401.32 mg vitamin C equivalents (VCE)/kg FW) and Yingsi (ABTS, IC 50 = 3.28 mg/mL; FRAP, 400.81 mg VCE/kg FW) showed better in vitro antioxidant capacity. Further cellular assay indicated that the common cultivar Dongkui (2 mg/mL) possessed the strongest ROS scavenging activity. The comprehensive evaluation of bioactive components and antioxidant properties using principal component analysis suggests that common cultivar Dongkui, new cultivars Yingsi and Anhaizaomei could be considered as dietary supplements.
研究领域:
食品科学
·
植物化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Chaoting Wen,Jialuo Tang,Dongming Liu,Meidi Fan,Xinying Lin,Guoyan Liu,Li Liang,Xiaofang Liu,Jixian Zhang,Youdong Li,Yuqing Duan,Xin Xu
In the study, Grifola frondosa polysaccharides (GFPs) and gallic acid (GA) were utilized to synthesize selenium nanoparticles (GFPs-GA-SeNPs) through a redox reaction involving sodium selenite and ascorbic acid. The morphological changes of GFPs-GA-SeNPs and their antioxidant activities during simulated gastrointestinal digestion were investigated. The results revealed that the antioxidant capacity of GFPs-GA-SeNPs was significantly enhanced compared to SeNPs and GFPs-SeNPs, with IC₅₀ values for scavenging DPPH, superoxide anion, and hydroxyl radical of 0.45, 0.55, and 0.63 mg/mL, respectively. In addition, GFPs-GA-SeNPs showed a two-stage release pattern in in vitro digestion and had a stronger selenium release capacity. At the cellular level, oxidative stress and apoptosis were aggravated in the model group compared with the control group. Interestingly, compared with SeNPs and GFPs-SeNPs, GFPs-GA-SeNPs can more effectively restore mitochondrial membrane potential, reduce ROS levels, and improve apoptosis. Besides, GFPs-GA-SeNPs can up-regulate the expression of Nrf2 protein ( p < 0.05) which can induce the expression of HO-1, SOD, GSH-Px, CAT, decrease the MDA content. Moreover, GFPs-GA-SeNPs can downregulate NF-κB protein, which can decrease the expression of TNF-α and increase the expression of IL-10. GFPs-GA-SeNPs demonstrated exceptional antioxidant activity and could benefit various industries, including food and health products, and have enormous potential application values.
研究领域:
食品科学
·
生物医学工程
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）