Environmental Science-Processes & Impacts
·
2021
·Vol. 23
·期 10
·1566-1577
·
IF 4.238
Cécilia Siri,Yang Liu,Thibault Masset,William Dudefoi,Dean Oldham,Matteo Minghetti,Dominique Grandjean,Florian Breider
The sorption of hydrophobic organic compounds (HOC) onto microplastics is relatively well reported in the literature, while their desorption remains poorly investigated, especially in biological fluids. The present study investigated the sorption and desorption of progesterone on polyethylene (PE), polypropylene (PP), and polystyrene (PS) microplastics. The sorption experiments showed that the equilibrium was reached in a few hours for all plastics. A sorption efficiency of 357.1 μg g−1 was found for PE and PS, and 322.6 μg g−1 for PP. Sorption experiments indicated that adsorption would certainly happen via surface sorption and a potentially pore-filling mechanism. The desorption was carried out in Simulated Gastric Fluid (SGF) and Simulated Intestinal Fluid (SIF), whose formulations were more complex than similar models reported so far. It has been found that the desorption was higher in SIF as compared to SGF, due to micelle formation in SIF promoting the pollutant solubilization. The sorption of pepsin onto microplastics has also been revealed, suggesting a competition between pollutants and pepsin for sorption sites and a potent reduction in pollutant solubilization. This study indicates that the ingestion of microplastics could be considered as an additional route of exposure to pollutants and therefore emphasizes pollutant bioavailability for aquatic organisms.
研究领域:
环境科学
·
毒理学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jie Yang,Yujing Sun,Xuyan Dong,Man Li,Yang Qin,Lei Dai,Qingjie Sun
This study investigates the interaction of amino-modified starch nanoparticles (NH 2 -SNPs) and unmodified SNPs with pepsin and trypsin and the influence of the formation of protein coronas on the release of polyphenols. We discovered that NH 2 -SNPs bound loosely to pepsin, while they bound tightly to trypsin, by quartz crystal microbalance with dissipation monitoring and zeta potential measurement. SNPs did not easily bind to the two digestive enzymes. In addition, the influence of NH 2 -SNPs on digestive enzymes was investigated by ultraviolet-visible spectrophotometry, and circular dichroism spectroscopy, showing that the addition of NH 2 -SNPs had no effect on the conformational structure of pepsin and trypsin. Using NH 2 -SNPs and SNPs to load four polyphenols revealed that the nanoparticles had a slow-release effect on the polyphenols, but the presence of protein coronas had little effect on the release. The release was mainly related to the destruction of the starch-based carrier by the amylase in digestive enzymes.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Yihui Wang,Yujing Sun,Jie Yang,Lei Dai,Na Ji,Liu Xiong,Qingjie Sun
Nanoparticles (NPs) can form a protein corona (PC) with proteins in biological fluids. We examined whether starch nanoparticles (SNPs) form a PC and interact with digestive enzymes in simulated gastric and intestinal fluids. We investigated the adsorption of pepsin and trypsin on unmodified, carboxyl-, and amino-modified SNPs (SNPs, COOH-SNPs, and NH2-SNPs, respectively). Quartz crystal microbalance data showed that a tight and irreversible pepsin corona formed on the NH2-SNPs, pepsin had little or no binding to the SNPs and COOH-SNPs, and trypsin had weak binding to all three kinds of NPs. Dynamic light scattering data showed that pepsin significantly increased the size of the NH2-SNPs from 120 ± 2.6 to 203 ± 12.2 nm and decreased their surface potential from 23.2 ± 1.0 to 12.7 ± 0.2 mV. NH2-SNPs could induce the fluorescence quenching of pepsin and change its secondary structures without affecting its activity.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Shaofeng Yuan,Changjian Li,Hang Yu,Yunfei Xie,Yahui Guo,Weirong Yao
Organophosphorus pesticides (OPPs) are a group of common residual pollutants in food that cause serious harm to human health. In the present study, the ability of 10 lactic acid bacteria (LAB) to degrade OPPs (dimethoate, parathion methyl, trichlorfon and chlorpyrifos) was explored for their potential application to degrade OPPs in fermented food . The results showed that some LAB can survive in the presence of OPPs and degrade them significantly in a short time. Among these, Lactiplantibacillus plantarum subsp. plantarum CICC20261 ( L. plantarum 20261) showed the highest degradation ability. The degradation behavior of OPPs by L. plantarum 20261 followed pseudo-zero-order kinetic model ( k = 1.0939–1.5915). Phosphatase produced by the strain played the key role in the degradation of OPPs because its activity was positively related to the degradation of OPPs ( r = 0.975–0.995). Phosphatase could degrade OPPs rapidly in vitro , further confirmed that L. plantarum 20261 degraded rather than adsorped the OPPs. In addition, the excellent antioxidant ability of LAB and the tolerance of simulated gastric and intestinal juices showed their potential protective effects against oxidative damage induced by pesticides in vivo .
研究领域:
食品科学
·
微生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Kefu Wang,Kangkang Wang,Yaoyao Chen,Siqi Liang,Yi Zhang,Changyan Guo,Wei Wang,Jide Wang
Microplastics (MPs) can enrich pollutants after being released into the environment, and the contaminants-loaded MPs are usually ingested by organisms, resulting in a potential dual biotoxic effect. In this paper, the adsorption behavior of Sulfamethoxazole (SMX) on Polyamide 6 (PA6) MPs was systematically investigated and simulated by the kinetic and isotherm models. The effect of environmental conditions (pH, salinity) on the adsorption process was studied, and the desorption behavior of SMX-loaded PA6 MPs was focused on simulating the seawater, ultrapure water, gastric and intestinal fluids. We found that lower pH and solubilization of SMX by gastrointestinal components (bovine serum albumin (BSA), sodium taurocholate (NaT), and pepsin) can reduce the electrostatic interaction between the surface charge of PA6 MPs and SMX. The result will lead to an increase in the desorption capacity of SMX-loaded PA6 MPs in gastrointestinal fluids and therefore will provide a reasonable mechanism for the desorption of SMX-loaded PA6 MPs in the gastrointestinal fluids. This study will provide a theoretical reference for studying the desorption behavior of SMX-loaded PA6 MPs under gastrointestinal conditions.
研究领域:
环境科学
·
生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Qihong Zhang,Hui Wang,Zongmiao Feng,Zhaohui Lu,Chen Su,Yunchun Zhao,Jingbo Yu,Alexandr V. Dushkin,Weike Su
As a functional polyphenolic compound, curcumin (CUR) has limited application due to its low solubility and bioavailability. In this work, curcumin loaded micelles self-assembled from disodium glycyrrhizin (Na 2 GA) was coated with pectin and tannic acid to construct a core-shell solid dispersion (SD-CUR). The assembly mechanism of the core-shell nanoparticle was clarified by molecular dynamics simulation. Improved solubility and in vitro release characteristics of SD-CUR in simulated gastric and intestinal juice were achieved. The parallel artificial membrane permeability (PAMPA) test confirmed that the permeability of curcumin in SD-CUR was significantly improved compared with the pure curcumin. Pharmacokinetic studies showed that the bioavailability was increased by 10 times. Intestinal in situ absorption study proved SD-CUR could enhance drug to transport across intestinal epithelial cells. Further hypolipidemic study demonstrated that the SD-CUR medication group could considerably reduce the levels of total cholesterol (TC), triglyceride (TG), and low density lipoprotein (LDL) in hyperlipidemia rats. These findings confirmed that SD-CUR had broad application prospects in the prevention and treatment of hyperlipidemia.
研究领域:
药剂学
·
分子动力学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
TRANSACTIONS OF TIANJIN UNIVERSITY
·
2015
·Vol. 21
·期 5
·383-391
·
IF 0
He Zhimin,Zhang Xiqing,Qi Wei,Huang Renliang,Su Rongxin
The aim of this work was to develop an alginate-casein composite microsphere as a bioactive vehicle for oral administration of nutrients by a simple extrusion dripping method. Riboflavin was selected as a model drug, and the microencapsulation efficiency was raised to 97.94% after optimizing the preparation conditions by response surface methodology. In vitro release studies showed that riboflavin was released completely from alginate-casein microspheres in simulated intestinal fluids. Meanwhile, the morphology, structure and interaction between alginate and casein were characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectra.
研究领域:
食品科学
·
药物递送系统
·
生物材料
✓ 已验证
产品关联
DOI
Food Bioscience
·
2025
·
IF 5.9
Chunting Du,Tao Wang,Dandan Li,Xiaohu Luo,Jiali Xing,Ren Wang,Hao Zhang
Oral administration of insulin is still a big challenge due to the harsh gastrointestinal environment. In this study, starch and chitosan were utilized to prepare insulin-loaded microcapsules with controllable releasing properties. Herein, the complexation between chitosan and insulin upon pH adjustment tended to form positively charged insulin nanoparticles (iNPs). At a chitosan/insulin ratio of 1:2, the diameter of iNPs and corresponding insulin loading capacity reached 196.2 nm and 0.70 mg/mg iNPs, respectively. Amylolytic hydrolysis of rice starch granules for 120 min could produce porous rice starch (PRs), enabling the encapsulation of iNPs to form iNPs-loaded PRs (PRs@iNPs). The iNPs were much easier encapsulated by PRs than the free insulin at an equivalent insulin concentration of 0.60 mg/mL. When PRs@iNPs mixed with different concentrations of sodium alginate, the calcium chloride initiated the cross-linking of calcium alginate, and the formation of calcium alginate layer not only protected the starch from the amylase hydrolysis, but also maintained the sustained release of insulin in the simulated gastrointestinal fluid. Moreover, both starch digestion and insulin releasing can be controlled by the concentration of sodium alginate used. This study may provide an efficient benign technology to produce microcapsules loaded with insulin nanoparticles for controllable releasing properties.
研究领域:
药剂学
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Lina Yang,Jinghang Huang,Xinghui Wu,Li Li,Wenqi Cai,Lijie Zhu,Shengnan Wang,Hong Song,Danshi Zhu,Tao Ma,He Liu
The purpose of this study was to investigate the effects of interactions between gut microbiota and soy hull polysaccharides (SHPs) on the air-liquid interfacial activity. SHPs could be used as an energy source for gut microbiota metabolism, and within a certain concentration range, the acid production of gut microbiota had a SHP concentration dependence. In the process of interfacial adsorption, the polysaccharide concentration was negatively correlated with interfacial tension, and the simulated intestinal fluid viscosity and dilational modulus were positively correlated. The low concentration of SHPs had a greater impact on the diversity and structural composition of gut microbiota. 0.5% SHPs had a better effect on the proliferation of Lactobacillus . The gut microbiota degraded SHPs involved in the regulation of interfacial activity, which provided a new theoretical foundation for the utilization and absorption of polysaccharides in the intestine in vitro .
研究领域:
微生物学
·
食品科学
·
生物物理学
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Qing Liu,Fang Li,Na Ji,Lei Dai,Liu Xiong,Qingjie Sun
Natural amphiphilic polymer micelles are of great interest as a carrier of hydrophobic functional ingredients due to their relatively easy synthesis, biocompatibility, and extensive sources. This study modified debranched starch (DBS) with acetic anhydride to introduce the hydrophobic acetyl group, and micelles were then obtained through the self-assembly of acetylated debranched starch (ADBS). The degree of substitution (DS) and the structural and thermal properties of ADBS were systematically investigated; the maximum DS value was 0.17. Proton nuclear magnetic resonance spectra of ADBS confirmed the presence of acetyl methyl protons, and Fourier transform infrared spectra exhibited a new absorption band of carbonyl C = O vibration at 1730 cm −1 due to the introduction of acetyl. The developed micelles were mostly spherical, and the particle size was about 50–100 nm. The critical micelle concentration was 0.45 mg/mL. Curcumin was incorporated into the micelles successfully, and the curcumin-loaded micelles exhibited sustained release properties in simulated gastric and intestinal fluids.
研究领域:
食品科学与工程
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Ben Chen,Dingwei Li,Bingjie Tong,Lifen Wang,Haoran Lin,Helin Xu,Sunkuan Hu
Ulcerative colitis (UC) is a chronic bowel inflammatory disease affecting the colorectum. Epidermal growth factor (EGF) has been demonstrated to be effective to counteract UC. However, there exists the gastrointestinal challenges such as stomach acid, enzyme and bile salts for oral delivery of EGF. Herein, calcium alginate microsphere was prepared by the microfluidic technique to encapsulate EGF. The morphology of EGF-loaded microsphere (MS-EGF) was spherical and its average particle size was 80 ± 23 μm. The encapsulation efficiency of EGF was reaching to 93.8 % ± 1.6 %. In vitro release experiments showed that MS-EGF presented the good pH-sensitive properties, that was, it could effectively resist the gastric acid and small intestinal fluids, and undergone the rapid dissolution in the artificial colon fluid. In vitro cellular experiments demonstrated that the bioactivity of EGF was well preserved by microsphere. Moreover, in vivo murine colitis model showed that MS-EGF presented the obvious colitis alleviation. Furthermore, the colonic morphology of colitis mice was effectively recovered and the tight junction between the gut epithelium was obviously repaired. In conclusion, calcium alginate microsphere might be a promising vehicle of EGF for UC treatment.
研究领域:
药学
·
胃肠病学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Food Research
·
2023
·
IF 0
Ziqi Xiao,Tianyu Tan,Shaoying Zhang,Jiaqi Tian,Tiandi Liu,Yandong Zhang,Tengfei Li
Pomegranate seeds are rich in polyphenols, known for their significant health benefits. In this study, we developed microcapsules to encapsulate pomegranate seed polyphenols (PSP) using a combination of zeaxylated protein (Zein) and chitosan (Cs). The optimization process involved a Zein to Cs volume ratio of 1:1, a chitosan concentration of 1.0 mg mL −1 , and a polyphenol concentration of 2.0 mg mL −1 . Scanning electron microscopy confirmed the formation of Zein/Cs/PSP complex coacervates, indicating the successful encapsulation. Fourier-transform infrared spectroscopy provided further evidence of electrostatic attraction between PSP and the Zein/Cs matrix. Differential scanning calorimetry analysis demonstrated the successful incorporation of PSP into the Zein/Cs wall material. Moreover, the encapsulated core material exhibited exceptional storage stability and retained its antioxidant activity. Notably, more than 85% of the encapsulated PSP remained intact after 5 h of simulated intestinal fluid digestion. This study underscores the potential of pomegranate seeds for comprehensive utilization in food engineering.
研究领域:
食品工程
·
材料科学
·
药学
✓ 已验证
DOI
危险品化学品经营许可证（带存储）