JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2022
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IF 9.965
Lijun Yang,Jiaxing Zhang,Mengfan Wang,Yutong Wang,Wei Qi,Zhimin He
As bridging species between short peptides and macromolecular proteins, peptide assemblies not only provide a supramolecular approach for the fabrication of controllable molecular machines with enzyme-like functions, but also a simplified model for understanding the catalytic mechanism of natural enzymes. In this study, we focused on probing the effect of microenvironment on the catalytic behavior of peptide assemblies. Upon simply replacing the X residue in Fmoc-FFXAH-CONH 2 , we realized the modulation of the microenvironment of the amyloid assemblies, which thus appeared esterase-like function with different catalytic abilities. The chemistry , structure and activity were analyzed to explore the principles that how the hydrophobic, charged, polar and chiral microenvironment deciding the catalytic behavior of the esterase mimic. In addition, we also presented the potential of the catalytic assemblies in the encapsulation, delivery and enzymatic metabolization of a mutual prodrug . This work sheds new insights for understanding the structure–function relationship of catalytic peptide assemblies and natural enzymes, and also provides a new avenue for the designing of artificial enzymes with better functions.
研究领域:
生物化学
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分子生物学
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催化科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Rongfang Yan,Ning Zhang,Weihua Liu,Xuelian Hu,Wenxiu Wang,Yiwei Tang,Shuo Wang,Xianghong Wang,Qinghai Sheng
Self-assembled Eu-dipeptide (tryptophan-phenylalanine) microparticles with multi-emission fluorescence was prepared and modified with a single-stranded DNA corresponding to the sulfamethazine (SMZ) adapter (Eu-PMPs@cDNA). Aptamer-functionalized magnetic Fe 3 O 4 (MNPs@aptamer) was used to specifically bind the target SMZ. Using Eu-PMPs@cDNA as fluorescent signal probe and MNPs@aptamer as catcher, a noncompetitive fluorescence sensing strategy was developed for determination of SMZ with good sensitivity, accuracy, selectivity, and stability. Under the optimized conditions, fluorescence increases linearly in the 0–20 ng/mL SMZ concentration range, and the detection limit is 0.014 ng/mL. The fluorescence sensing method was applied to analysis of water and fish muscle samples, and recoveries ranged from 81.78 to 119.46 % with relative standard deviations below 4.2 %. This study offered a reliable and sensitive fluorescence sensing strategy for SMZ determination in food samples, which owns great potential for wide-ranging application in harmful compounds assay by simply changing the type of aptamer and its complementary single-stranded DNA.
研究领域:
分析化学
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食品科学
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生物医学工程
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2018
·Vol. 7
·期 6
·915-926
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IF 4.776
Hong Liang,Ao Hu,Xiaobing Chen,Rongrong Jin,Kefeng Wang,Bowen Ke,Yu Nie
Disulfide modified lipopeptide assemblies with an arginine-rich dendritic periphery provide a promising platform for effective gene transfer. Dendritic arginine peptides that mimic the cell-penetrating peptides of a virus envelope are vital for complexation, interaction with physical barriers, and final gene release. Here, we report three lipopeptides with different-generation dendritic peripheries (R1LS, R2LS and R3LS), each of which contains a dioleoyl-L-lysinate hydrophobic tail. Such molecules were proven to self-assemble in aqueous solution with different morphologies, sizes, and surface zeta potentials. R2LS and R3LS assemblies showed spherical and spindle shapes with zeta potentials of 27.2 and 32.8 mV, respectively. They exhibited complete condensation of pDNA at a low N/P ratio, while R1LS assemblies displayed a fiber pattern with a relatively low electric potential of 10.9 mV with poor DNA binding ability. In a cellular viability experiment, R1LS and R2LS have no significant cytotoxicity even at high dosage, while R3LS showed conspicuous toxicity. As a gene vector, R2LS presented high gene transfection efficiency either in the presence or the absence of serum, which was 58.7% greater than liposome 2000 and PEI in the condition of 10% fetal bovine serum for HeLa cells. While R3LS showed good results just without serum and R1LS was unserviceable in all situations. Moreover, molecular dynamic simulation was exploited to analyze the kinestate of the signal molecule and the interactions of multiple molecules, which could assist us in better understanding the experimental phenomena. The simulation results indicated that the R2LS molecule has better flexibility, which was favorable for interaction with the cell membrane. And it could generate tight integration in self-assembly while R1LS and R3LS assemblies have a large molecular interval, which led to a controllable release of cargos for R2LS in a reductive environment. In summary, the generation of the dendrimer in lipopeptides is vital for the gene transfer effect. For optimization, it is necessary to study the structure–function relationship, and molecular dynamic simulation is an effective strategy for screening the molecular structure and even for predicting experimental results.
研究领域:
生物化学
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分子生物学
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药剂学
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DOI
PubMed
高影响因子
Science Advances
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2024
·Vol. 10
·期 37
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IF 11.7
Dinghao Chen,Ziao Zhou,Nan Kong,Tengyan Xu,Juan Liang,Pingping Xu,Bingpeng Yao,Yu Zhang,Ying Sun,Ying Li,Bihan Wu,Xuejiao Yang,Huaimin Wang
Current pharmacotherapy remains futile in acute alveolar inflammation induced by Gram-negative bacteria (GNB), eliciting consequent respiratory failure. The release of lipid polysaccharides after antibiotic treatment and subsequent progress of proinflammatory cascade highlights the necessity to apply effective inflammation management simultaneously. This work describes modular self-assembling peptides for rapid anti-inflammatory programming (SPRAY) to form nanoparticles targeting macrophage specifically, having anti-inflammation and bactericidal functions synchronously. SPRAY nanoparticles accelerate the self-delivery process in macrophages via lysosomal membrane permeabilization, maintaining anti-inflammatory programming in macrophages with efficacy close to T helper 2 cytokines. By pulmonary deposition, SPRAY nanoparticles effectively suppress inflammatory infiltration and promote alveoli regeneration in murine aseptic acute lung injury. Moreover, SPRAY nanoparticles efficiently eradicate multidrug-resistant GNB in alveoli by disrupting bacterial membrane. The universal molecular design of SPRAY nanoparticles provides a robust and clinically unseen local strategy in reverse acute inflammation featured by a high accumulation of proinflammatory cellularity and drug-resistant bacteria.
研究领域:
药理学
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纳米技术
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传染病学
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肺科学
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免疫学
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DOI
PubMed
高影响因子
BIOMACROMOLECULES
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2017
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IF 5.246
Jing Xu,Xiangyi Li,Xiaodong Li,Bao Li,Lixin Wu,Wen Li,Xiaoming Xie,Rong Xue
Peptide assembly has reached exquisite levels of efficiency in the creation of bioactive materials. However, we have not yet been able to take what we have learned from peptide assembly to develop a general strategy for the fabrication of biomimetic underwater adhesives, which retain significant advantages as medical glue for clinical treatment. Herein we report a simple approach to prepare peptide-based adhesives through the supramolecular polymerization of cationic peptides drove by polyoxometalates (PMs). Mass spectra, Fourier-transform infrared spectra and 183W NMR spectra confirmed the structural integrity of peptides and PMs during the coassembly process. Scanning electron microscopy demonstrated that the multivalent interactions between peptides and polyoxometaltes led to the formation of robust 3D network structures. The rheological study revealed that the peptide/PM assemblies exhibited mechanically rigid gel-like behavior and self-healing property. Interestingly, the assemblies showed the capacity to adhere various wet solid materials under waterline. The shear strength of the peptide-based adhesives are stronger than that of the commercially available fibrin glue. This finding is exciting and serves to expand our capability of the fabrication of peptide-based materials.
研究领域:
材料科学
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生物材料
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化学
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生物医学工程
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高分子科学
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产品关联
DOI
PubMed
高影响因子
Advanced Science
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2024
·
IF 14.3
Huiyan Du,Ensheng Xu,Yihan Xu,Qingwang Xue,Hongxia Xu,Jibin Song
The development of theragnostic nanosystems integrating FRET (fluorescence resonance energy transfer) imaging and chemodynamic therapy (CDT) for accurate diagnosis and effective treatment of lung tumors is still a big challenge. Herein, a peptide-assembled 3D DNAzyme motor nanodevice is engineered for a self-powered FRET amplifier profiling human neutrophil elastase (HNE) and self-supplied H 2 O 2 enhancing CDT. The nanodevice is prepared by depositing AuNPs on ZIF-8, in which ZIF-8 co-loaded the lysosomal targeting peptide-modified copper peroxides (PCPs) and hairpins (H1, H2, and H3), AuNPs are co-labeled by DNAzyme-peptide (DP) conjugate and H3. In the tumor micro-environment, HNE driven 3D DNAzyme walker followed by an exponential amplification constructed by a synergistic cross-activation between hybridization chain reaction and DNAzyme, generating a self-powered FRET amplifier. The FRET amplifier specifically measures HNE with a sensitivity of 0.026 pM, and successfully images exogenous HNE in living cells and monitors HNE in mouse models. Moreover, the PCPs can target lysosomes, reducing lysosome escape. The self-supplying H 2 O 2 undertaken by PCPs improves the Cu (II)-catalyzed Fenton-like reaction, effectively causing cell apoptosis to inhibit tumor growth. Significantly, the nanodevice successfully screens inhibitors and discriminates the HNE level in normal and lung cancer tissues, suggesting that the nanodevice provides an effective tool for the diagnosis and treatment of lung tumors.
研究领域:
纳米技术
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癌症诊断与治疗
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分子生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
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