Foods
·
2021
·Vol. 10
·期 12
·2976
·
IF 4.35
Jianying Zhao,Jing Qian,Hong Zhuang,Ji Luo,Mingming Huang,Wenjing Yan,Jianhao Zhang
This study aimed to investigate effects of plasma-activated solution (PAS) on the cell biology ofStaphylococcus aureusand qualities of fresh lettuce leaves. PAS was prepared by dielectric barrier discharge plasma and incubated withS. aureusfor 10–30 min or with lettuces for 10 min. Effects on cell biology were evaluated with microscopic images, cell integrity, and chemical modification of cellular components. Effects on lettuce quality were estimated with the viable microbial counts, color, contents of vitamin C and chlorophyll, and surface integrity. PAS reducedS. aureuspopulation by 4.95-log and resulted in increased cell membrane leakage. It also resulted in increased contents of reactive oxygen species in cells, C=O bonds in peptidoglycan, and 8-hydroxydeoxyguanosine content in cellular DNA, and reduced ratios of unsaturated/saturated fatty acids in the cell membrane. PAS treatment reduced bacterial load on fresh lettuce and had no negative effects on the quality. Data suggest that PAS can be used for the disinfection of ready-to-eat fresh vegetables.Keywords:reactive oxygen species;peptidoglycan;cell membrane;DNA;lettuces
研究领域:
食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Toxics
·
2024
·Vol. 12
·期 6
·380
·
IF 3.9
Hui Yang,Xue Zhang,Yadong Zhu,Bo Zhang,Junfeng Fan,Hongfei Zhao,Bolin Zhang
Acrylamide (AA) and 5-hydroxymethylfurfural (HMF), which are potentially carcinogenic to humans, are often produced during the hot processing of foods. This study first used a molecular docking model to simulate the binding behavior of four lactic acid bacteria peptidoglycans (PGNs) to AA/HMF, and the binding rate of LAB-based PGNs to AA/HMF was evaluated in vitro. In silico results show that interaction energy is the driving force responsible for the adsorption of LAB-derived PGNs to AA/HMF. In vitro results showed that the PGN ofB. lactisB1-04 bound the most AA (28.7%) and HMF (48.0%), followed byL. acidophilusNCFM,B. breveCICC 6079, andL. plantarumCICC 22135. Moreover, an AA/HMF-bound layer on the cell surface ofB. lactisB1-04 was observed via AFM and SEM due to adsorption. XPS analysis indicated the removal rate of AA/HMF by selected strains was positively correlated with the proportion of C-O, C=O, and N-H groups of PGNs. The atoms O1, O2, O3, O4, N1, N2, N3, H1, and H2 are involved in the adsorption of LAB-based PGNs to AA/HMF. Thus, the PGNs derived from these fourLactobacillusstrains can be regarded as natural adsorbents for the binding of AA/HMF.
研究领域:
食品科学
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微生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yanbei Zhang,Jie Li,Shoulei Yan
Lactic acid bacteria fermentation significantly enhances the color quality of fruit and vegetable juices and delays non-enzymatic browning. Nevertheless, the underlying mechanisms had rarely been reported and the existing ones were only relatively superficial studies from the perspective of browning substrates. Therefore, the mechanism of lactic acid bacteria fermentation mitigating the browning of lotus rhizome juice was investigated from the perspective of browning product removal in this research. Results showed lactic acid bacteria improved the color of lotus rhizome juice by adsorbing browning pigment with 53.42 %. Peptidoglycan was the key site for pigment adsorption, with this capability being intricately linked to its composition and structure. Electrostatic and hydrophobic forces were involved in browning pigment adsorption. Notably, cell viability was not a factor that affected pigment adsorption. In the future, inactivated lactic acid bacteria can be widely used as biosorbents to enhance the color of unfermented food.
研究领域:
食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Chang’e Wang,Junjian Miao,Chang Liu,Yi Guo,Lili Zhang,Ying Lu
Peptidoglycan (PG) is a key component that enables lactic acid bacteria to remove tetrodotoxin (TTX). However, the adsorption behaviors and interaction mechanisms between PG and TTX remain unclear. Herein, three types of PGs (A3α-PG, A1γ-PG, and A4α-PG) were used to treat TTX. After treatment, the residual TTX content was reduced by 82.85 %–84.73 %, and TTX toxicity decreased by 87.30 %–93.02 %, indicating that the three PGs could effectively remove TTX. Adsorption kinetics analysis showed that the adsorption of TTX by the three PGs conformed to the quasi-second-order ( R 2 > 0.99) and the Freundlich ( R 2 > 0.99) models. These findings suggest that the adsorption behavior between PG and TTX involves a coexistence of chemical adsorption and multilayer physical adsorption modes. Furthermore, molecular docking simulations and quantum chemical calculations clarified that the adsorption interaction between PG and TTX involved physical interactions mediated by hydrophobic interactions and van der Waals forces, as well as chemical interactions mediated by strong hydrogen bonds (1.352 Å) with a Mayer bond order of 0.2687. This study elucidates the mechanism of PG in removing TTX, providing a theoretical basis for developing more efficient TTX adsorbents and detoxification technologies in the future. Further research on the adsorption kinetics and interactions between PG and TTX may offer methodological references and novel insights into the adsorption mechanisms of other biological molecules.
研究领域:
微生物学
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生物化学
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食品安全
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生物物理学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Jian Zhang,Haochen Qi,Jie Jayne Wu,Xuanjiao Mao,Hailin Zhang,Niloufar Amin,Feng Xu,Changkun Dong,Chunchang Wang,Pengjun Wang,Lei Zheng
Rapidly identifying and quantifying Gram-positive bacteria are crucial to diagnosing and treating bacterial lower respiratory tract infections (LRTIs). This work presents a field-deployable biosensor for detecting Gram-positive bacteria from exhaled breath condensates (EBCs) based on peptidoglycan recognition using an aptamer. Dielectrophoretic force is employed to enrich the bacteria in 10 s without additional equipment or steps. Concurrently, the measurement of the sensor’s interfacial capacitance is coupled to quantify the bacteria during the enrichment process. By incorporation of a semiconductor condenser, the whole detection process, including EBC collection, takes about 3 min. This biosensor has a detection limit of 10 CFU/mL, a linear range of up to 105 CFU/mL and a selectivity of 1479:1. It is cost-effective and disposable due to its low cost. The sensor provides a nonstaining, culture-free and PCR-independent solution for noninvasive and real-time diagnosis of Gram-positive bacterial LRTIs.
研究领域:
医学诊断
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生物传感
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微生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2016
·Vol. 51
·期 3
·664-671
·
IF 1.504
Zhen Wu,Daodong Pan,Xiaoqun Zeng,Yangying Sun,Jinxuan Cao
Summary Peptidoglycan (PG) is available from a wide variety of lactic acid bacteria (LAB) and is the main structure of cell wall components. Phosphorylated modification would bring new properties such as the potential antioxidant activities and antiviral capability to an organic molecule. In the present work, small molecular fragments of PG (derived from Lactobacillus acidophilus ) hydrolysed by mutanolysin were phosphorylated under optimal conditions. P-PG had a monomer molecular structure of GlcNAC[PO 3 ]–MurNAC–Ala–Glu–Lys–Ala, with a molecular mass of 884 Da and a phosphorus content of 8.9%. P-PG displayed some immunoregulatory activity in lipopolysaccharides (LPS) stimulated RAW 264.7 macrophages. Compared with the LPS-stimulated group, the addition of P-PG inhibited the secretion of GM-CSF, TNF-α and IL-1 in a dose-dependent manner. The effect of 50 μg mL −1 of P-PG was more significant than 50 μg mL −1 of PG. Lower fluorescence of lysosomes was observed in P-PG-treated RAW 264.7 cells may also reveal some immune defence function in the LPS-induced macrophages.
研究领域:
微生物学
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免疫学
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分子生物学
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产品关联
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·Vol. 107
·期 22
·6985-6998
·
IF 5
Zhang Mengmei,Chen Yuan,Lai Jinghui,Wang Xingjie,Hu Kaidi,Li Jianlong,Li Qin,He Li,Chen Shujuan,Liu Aiping,Ao Xiaolin,Yang Yong,Liu Shuliang
Abstract The presence of cypermethrin in the environment and food poses a significant threat to human health. Lactic acid bacteria have shown promise as effective absorbents for xenobiotics and well behaved in wide range of applications. This study aimed to characterize the biosorption behavior of cypermethrin by Lactiplantibacillus plantarum RS60, focusing on cellular components, functional groups, kinetics, and isotherms. Results indicated that RS60 exopolysaccharides played a crucial role removing cypermethrin, with the cell wall and protoplast contributing 71.50% and 30.29% to the overall removal, respectively. Notably, peptidoglycans exhibited a high affinity for cypermethrin binding. The presence of various cellular surface groups including –OH, –NH, –CH 3 , –CH 2 , –CH, –P = O, and –CO was responsible for the efficient removal of pollutants. Additionally, the biosorption process demonstrated a good fit with pseudo-second-order and Langmuir–Freundlich isotherm. The biosorption of cypermethrin by L. plantarum RS60 involved complex chemical and physical interactions, as well as intraparticle diffusion and film diffusion. RS60 also effectively reduced cypermethrin residues in a fecal fermentation model, highlighting its potential in mitigating cypermethrin exposure in humans and animals. These findings provided valuable insights into the mechanisms underlying cypermethrin biosorption by lactic acid bacteria and supported the advancement of their application in environmental and health-related contexts. Key points
• Cypermethrin adsorption by L. plantarum was clarified.
• Cell wall and protoplast showed cypermethrin binding ability.
• L. plantarum can reduce cypermethrin in a fecal fermentation model.
研究领域:
环境科学
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微生物学
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食品科学
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毒理学
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产品关联
DOI
PubMed
高影响因子
Biomed Research International
·
2018
·
IF 2.583
Shumei Wang,Xue Han,Lanwei Zhang,Yingchun Zhang,Hongbo Li,Yuehua Jiao
The Lactobacillus paracasei subsp. paracasei M5 strain exerted potential anticancer activity through the cell wall. In this study, whole peptidoglycan (WPG) was extracted from the Lactobacillus paracasei subsp. paracasei M5 strain and was evaluated for anticancer effects as well as its properties. SDS-PAGE analysis confirmed the presence of WPG with dominant bands of approximately 14.4 kDa. Further analysis revealed that the amino acids present in the WPG consisted of alanine, glycine, glutamic acid, and lysine in a molar ratio of approximately 8 : 5 : 3 : 3.5. In addition, the cell viability of HT-29 cells with WPG addition was investigated with methyl thiazolyl tetrazolium (MTT) and trypan blue exclusion (TBE) assays, and cell apoptosis was analyzed with a transmission electron microscope, flow cytometry, and semiquantitative RT-PCR. These results showed that WPG exerted cytotoxic effects on colon cancer HT-29 cells in a dose-dependent manner and upregulated proapoptotic genes, while downregulating antiapoptotic genes. The gene expression study definitively revealed that WPG induced a mitochondria-mediated apoptotic pathway.
研究领域:
微生物学
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癌症生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROBIOLOGICAL RESEARCH
·
2025
·
IF 6.9
Zheng Jia,Lingdi Niu,Junjie Guo,Jiaqing Wang,Hai Li,Runhang Liu,Ning Liu,Shuhe Zhang,Fang Wang,Junwei Ge
Regulation of the innate immune response may be an effective strategy to enhance Staphylococcus aureus vaccines. Based on our previous findings that the Listeria peptidoglycan skeleton (pBLP) enhances the immune response through an unknown mechanism, we hypothesized that pBLP provides protection by modulating the innate immune response via trained immunity. In vitro, pBLP increased phagocytosis and inflammatory cytokine levels and elevated the anti-inflammatory cytokine TGF-β following secondary stimulation. In an in vivo model, our findings indicate that pBLP, when administered with a vaccine, protects mice from methicillin-resistant S. aureus challenge and also provides protection against S. aureus CMCC26003 in the absence of antigens. Using an ex vivo model, we demonstrated that pBLP increases markers of trained immunity in peritoneal macrophages. Transcriptome analysis of differentially expressed genes and inhibitor experiments revealed that the trained immunity process induced by pBLP depends on mTOR-HIF-1α and hexokinase 2. This study is the first to demonstrate that pBLP can induce trained immunity. Furthermore, we show that the peptidoglycan skeleton induces a distinct trained immunity phenotype compared to β-glucan, enhancing vaccine protection. Our study provides valuable insights for the design of novel vaccines that integrate both specific and innate immune responses.
研究领域:
免疫学
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微生物学
·
疫苗研发
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产品关联
DOI
PubMed
高影响因子
BIOTECHNOLOGY AND BIOENGINEERING
·
2024
·
IF 3.5
Mingyuan Tang,Yao Lei,Kehan Chen,Mingming Ding,Qian Ou,Jing Tang,Yunwen Zhang,Tian Tang,Chuan Wang
Listeria monocytogenes (LM) is a Gram-positive (G + ) bacterium that secretes nanoscale membrane vesicles (MVs). LM MVs comprise various bacterial components and may have potential as an antigen or drug-delivery vehicle; however, the low yield of the LM MVs limits related research. G + -bacterial MVs germinate from the bacterial plasma membrane and must pass through a thick crosslinked peptidoglycan layer for release. Herein, we aimed to increase the release of MVs by reducing the degree of crosslinking of peptidoglycan. We knocked out two genes related to the longitudinal crosslinking of peptidoglycan, dal and dat , and supplemented the knocked-out dal gene through plasmid expression to obtain a stably inherited recombinant strain LMΔ dd ::pCW633. The structure, particle size, and main protein components of MVs secreted by this recombinant strain were consistent with those secreted from the wild strain, but the yield of MVs was considerably increased ( p < 0.05). Furthermore, Listeria ivanovii (LI) was found to secrete MVs that differed in the composition of the main proteins compared with those of LM MVs. The abovementioned method was also feasible for promoting the secretion of MVs from the attenuated LM strain and LI wild-type and attenuated strains. Our study provides a new method to increase the secretion of MVs derived from Listeria that could be extended to other G + bacteria.
研究领域:
微生物学
·
细菌学
·
生物技术
·
药学
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DOI
PubMed
高影响因子
ENGINEERING IN LIFE SCIENCES
·
2019
·Vol. 19
·期 4
·270-278
·
IF 1.936
Jinyuan Hu,Xiao Lu,Haokun Wang,Fuxiang Wang,Yuan Zhao,Wei Shen,Haiquan Yang,Xianzhong Chen
d -Alanyl- d -alanine carboxypeptidase DacC is important for synthesis and stabilization of the peptidoglycan layer of Escherichia coli . In this work, dacC of E . coli BL21 (DE3) was successfully deleted, and the effects of this deletion on extracellular protein production in E . coli were investigated. The extracellular activities and fluorescence value of recombinant amylase, green fluorescent protein, and α-galactosidase of the deletion mutants were increased by 82.3, 29.1, and 37.7%, respectively, compared with that of control cells. The outer membrane permeability and intracellular soluble peptidoglycan accumulation of deletion mutant were also enhanced compared with those of control cells, respectively. Based on fluorescence-assisted cell sorting analyses, we found that the morphology of the E. coli deletion mutant cells was altered compared with that of control cells. Local transparent bulges in the poles of the E . coli mutant with deletion of the dacC gene were found by transmission electron microscopy analysis. These bulges in the poles could explain the improvement in the production of extracellular protein by the E. coli mutant with deletion of the dacC gene. These findings provide important insights into the extracellular production of proteins using E . coli as microbial cell factories.
研究领域:
微生物学
·
生物技术
·
分子生物学
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DOI
PubMed
高影响因子
FITOTERAPIA
·
2023
·
IF 3.4
Saijuan Li,Fangge Wu,Pengcheng Gao,Can Jin,Yuyong Wang,Wenfeng Liao,Kan Ding
The accumulation of amyloid β (Aβ) containing senile plaques is one of the key histopathological hallmarks of Alzheimer's disease (AD). Increasing evidences demonstrated the important role of autophagy in Aβ clearance. Recent studies implied that extracts from Semiaquilegia adoxoides (DC.) Makino could ameliorate the memory of D-galactose induced aging mice. However, the bioactive substance and underlying mechanism remains unknown. Thus, the present study sought to explore the effects of a novel homogenous peptidoglycane on Aβ 42 secretion and the underlying mechanism. Briefly, we extracted a novel peptidoglycan named SA02C using hot water extraction and alcohol precipitation with the Mw of 13.72 kDa. SA02C contains 73.33% carbohydrate and 27.83% protein. The structure characterization revealed that its glycan part might mainly composed of galacturonic acid with minor rhamnose in backbone, and branched with glucose, galactose, arabinose, xylose and galacturonic acid. The protein or peptide moiety in SA02C was bonded to the polysaccharide via threonine. Bioactivities test showed that SA02C could reduce Aβ 42 production in a dose dependent manner with no obvious cytotoxicity. Mechanism study demonstrated that SA02C could modulate APP processing by upregulating the expression of ADAM10, sAPPα and downregulating BACE1, sAPPβ. Furthermore, SA02C also could stimulate autophagy by promoting the expression of the markers of autophagy such as LC3B and ATG5, resulting in the promotion of Aβ 42 phagocytosis.
研究领域:
药理学
·
神经科学
·
分子生物学
·
植物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）