MICROCHEMICAL JOURNAL
·
2018
·
IF 2.746
Yifan Zhang,Bei Wang,Huayu Xiong,Wei Wen,Nan Cheng
A ratiometric fluorescence nanoprobe, consisted of water-soluble fluorescent carbon dots (CDs) and 3‑mercapropionic acid-coated cadmium telluride quantum dots (CdTe QDs), was constructed for the detection of epinephrine (EP) and norepinephrine (NE). Under single-wavelength excitation (370 nm), the hybrid nanoprobe possessed dual emission peaks at 460 and 610 nm belongs to CDs and CdTe QDs, respectively. CdTe QDs fluorescence was quenched by EP or NE via electron transfer from QDs to the oxidation products of catecholamines , while the fluorescence intensity of CDs remained almost constant, which provided a ratiometric fluorescence assay for the catecholamines detection. The relative fluorescence intensity ratios were directly proportional to the concentration of catecholamines. Meanwhile, some structural analogues of the target molecules, including dopamine hydrochloride and isoprenaline hydrochloride, had no significant interference for the detection mode. The novel fluorescence detection platform was rapid and convenient, and applied to the detection of EP and NE in human serum samples with satisfactory results.
研究领域:
生物化学
·
分析化学
·
医学检测
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DOI
高影响因子
Genes
·
2022
·Vol. 13
·期 7
·1113
·
IF 4.141
Biying Jiang,Baolong Cao,Zhichun Zhou,Zejiao Li,Can Lv,Jiannan Zhang,Heyuan Zhang,Yajun Wang,Juan Li
Adrenoceptors are suggested to mediate the functions of norepinephrine (NE) and epinephrine (EPI) in the central nervous system (CNS) and peripheral tissues in vertebrates. Compared to mammals, the functionality and expression of adrenoceptors have not been well characterized in birds. Here, we reported the structure, expression, and functionality of chicken functional α2A-adrenoceptor, namedADRA2A. The cloned chickenADRA2AcDNA is 1335 bp in length, encoding the receptor with 444 amino acids (a.a.), which shows high amino acid sequence identity (63.4%) with its corresponding ortholog in humans. Using cell-based luciferase reporter assays and Western blot, we demonstrated that the ADRA2A could be activated by both NE and EPI through multiple signaling pathways, including MAPK/ERK signaling cascade. In addition, the mRNA expression ofADRA2Ais found to be expressed abundantly in adult chicken tissues including thyroid, lung, ovary and adipose from the reported RNA-Seq data sets. Moreover, the mRNA expression ofADRA2Ais also found to be highly expressed in the granulosa cells of 6–8 mm and F5 chicken ovarian follicles, which thus supports that ADRA2A signaling may play a role in ovarian follicular growth and differentiation. Taken together, our data provide the first proof that the α2A-adrenoceptor is functional in birds involving avian ovarian follicular development.Keywords:chickens;α2-adrenoceptor;ADRA2A
研究领域:
分子生物学
·
内分泌学
·
生殖生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 4
·1417-1423
·
IF 4.831
Xiao Deli,Liu Shubo,Liang Liyun,Bi Yanping
Epoxy propyl bonded magnetic microspheres were prepared by atomic layer deposition using Fe 3 O 4 @SiO 2 microspheres as a core support material. Then, a restricted-access magnetic sorbent was prepared that contains diol groups on the external surface and m-aminophenylboronic acid groups on the internal surface. This kind of microspheres achieved excellent specific adsorption of the ortho-dihydroxy compounds (dopamine, adrenaline and noradrenaline). Following desorption with sorbitol, the ortho-dihydroxy compounds were quantified by HPLC. The limits of detection for dopamine, adrenaline and noradrenaline were 0.074, 0.053 and 0.095 μg mL −1 , respectively. Recoveries from spiked mice serum samples range from 80.2 to 89.1 %.
A magnetic restricted-access sorbent with diol groups on the external surface and m-aminophenylboronic acid groups on the internal surface was synthesized for highly selective extraction of adrenaline, dopamine and noradrenaline from biological samples.
研究领域:
分析化学
·
生物医学工程
·
药物分析
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Ying Zhang,Wang Ren,Yu Zhu Fan,Hong Qun Luo,Nian Bing Li
Monitoring the concentration of norepinephrine (NE), epinephrine (Ep) and dopamine-β-hydroxylase (DβH) is of great importance in studying and diagnosing related diseases. In this study, a simple chemically-modulated turn-on fluorescence strategy is proposed for rapid, selective and visual discrimination of trace NE and Ep. Herein, Ep is differentiated from other catecholamines via its fast fluorescence response in 1.0 M NaOH medium. Furthermore, NE is alkalized with 0.01 M NaOH and is then immediately triggered by polyethyleneimine to emit bright cyan fluorescence, whereas Ep and DA show no any fluorescence response. The fluorescence intensity of resultant solution is linearly proportional to the concentration of Ep and NE in the range of 50.0–10000.0 nM and 10.0–10000.0 nM, respectively. Moreover, NE and Ep as low as 500.0 nM can be fluorescently identified with naked-eye, which is convenient for rapidly preliminary diagnosis and self-test of hypercatecholaminism. Inspired by the catalytic property of DβH transforming DA into NE, the proposed strategy enables a simple and high-efficient fluorescence method to monitor DβH by using trace DA as substrate and directly quantifying NE. The method is simple, smart, highly efficient and eco-friendly, and has been applied to the rapid assay of NE, Ep and DβH in real samples.
研究领域:
生物化学
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医学诊断
·
分析化学
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DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2019
·
IF 2.983
Huijie Lv,Siqi Luo,Ruochen Tian,Wenhua Zhang,Baoshan Sun,Yan Cui
Epinephrine and norepinephrine are a class of chiral endogenous catecholamines, which are known as major neurotransmitters. This work described a new LC–MS/MS method coupled with pre-column derivatization, enabling the simultaneous enantiomeric separation of epinephrine and norepinephrine in rat plasma. After protein precipitation procedure, the samples were derivatized with ( S )-N-(4-nitrophenoxycarbonyl) phenylalanine methoxyethyl ester, [( S )-NIFE]. The derivatives resolved with good baseline separation on an ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with mobile phase composed of methanol with 0.2% formic acid in water at a flow rate of 0.2 mL/min. Analysis was performed by multiple reaction monitoring in positive ionization mode. The linear ranges were 1.0–500 ng/mL for epinephrine enantiomers and 1.5–750 ng/mL for norepinephrine enantiomers. The lower limits of quantification for epinephrine and norepinephrine enantiomers were 1.0 and 1.5 ng/mL, respectively. The intra-day and inter-day precision were all less than 10.7% and accuracy ranged from 96.0 to 101.5%. Recoveries for all the analytes were more than 80.3%. The proposed method was successfully applied to simultaneously determine endogenous epinephrine and norepinephrine enantiomers in rat plasma. l -epinephrine and l -norepinephrine were sensitively and accurately quantified while both the d -enantiomers were not detected. Additionally, epinephrine enantiomers were analyzed for stereoselective pharmacokinetics in rats after intravenous administration of racemic epinephrine for the first time. The pharmacokinetic results indicated that the disposition of epinephrine enantiomers was stereoselective and chiral inversion did not occur in rats.
研究领域:
分析化学
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药物代谢
·
神经生物学
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DOI
高影响因子
Communications Biology
·
2025
·Vol. 8
·期 1
·1-12
·
IF 5.1
Yuan Xingyue,Li Ya,Cong Lin,Yang Lingling,Zhang Yangyang,Zhang Zhenzhen,Wang Ting,Dong Muchen,Du Xianli,Xie Lixin,Zhou Qingjun
Norepinephrine (NE) is mainly released by sympathetic nerve terminals to act on organs and tissues. After corneal epithelial debridement, we found that the sympathetic nerve fibers penetrated the limbus and regenerated toward the cornea within 24 h post-wounding. Topical NE application recapitulated the characteristics of delayed corneal epithelial wound healing and nerve regeneration in healthy mice, accompanied by the partial depletion of multiple neurotrophins, such as nerve growth factor and glial cell-derived nerve growth factor. Moreover, the diabetes mellitus (DM) mice exhibited corneal sensory nerve dysfunction and increased plasma and corneal NE contents, which were rescued by 6-hydroxydopamine (6-OHDA) and bretylium. In the cell culture model, the conditioned medium of NE-treated corneal epithelial cells inhibited trigeminal ganglion (TG) neurite outgrowth, which was reversed by the β2 adrenergic receptor (ADRB2) antagonist, but not by the β1 adrenergic receptor (ADRB1) antagonist. Topical application of the ADRB2 antagonist recovered the expression of corneal neurotrophins, and promoted corneal epithelial and nerve regeneration in DM mice. Taken together, the NE-ADRB2 axis regulates corneal neurotrophin expression and nerve regeneration in mice. Topical application of the ADRB2 antagonist may represent a promising therapeutic strategy for diabetic corneal sensory nerve dysfunction.
研究领域:
神经生物学
·
眼科
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 11
·3520
·
IF 4.927
Hongyu Zeng,Xiaoqing Zhang,Qianna Zhen,Yifan He,Haoran Wang,Yang Zhu,Qi Sun,Min Ding
A novel dual-template magnetic molecularly imprinted polymer (MMIP) was synthesized to extract normetanephrine (NMN), metanephrine (MN) and 3-methoxytyramine (3-MT) from spot urine samples. As the adsorbent of dispersive solid-phase extraction (d-SPE), the MMIP was prepared using dopamine and MN as dual templates, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the crosslinking reagent and magnetic nanoparticles as the magnetic core. NMN, MN, 3-MT and creatinine (Cr) in spot urine samples were selectively enriched by d-SPE and detected by HPLC-fluorescence detection/ultraviolet detection. The peak area (A) ratios of NMN, MN and 3-MT to Cr were used for the diagnosis of pheochromocytomas and paragangliomas (PPGLs). The results showed that the adsorption efficiencies of MMIP for target analytes were all higher than 89.0%, and the coefficient variation precisions of intra-assay and inter-assay for the analytes were within 4.9% and 6.3%, respectively. The recoveries of the analytes were from 93.2% to 112.8%. The MMIP was still functional within 14 days and could be reused at least seven times. The d-SPE and recommended solid-phase extraction (SPE) were both used to pretreat spot urine samples from 18 PPGLs patients and 22 healthy controls. The correlation coefficients of ANMN/ACrand AMN/ACrbetween d-SPE and SPE were both higher than 0.95. In addition, the areas under the receiver operator curves for spot urine ANMN/ACr, AMN/ACrand plasma free NMN and MN were 0.975, 0.773 and 0.990, 0.821, respectively, indicating the two methods had the similar performances. The d-SPE method took only 20 min, which was effective in clinical application.Keywords:magnetic molecularly imprinted polymer;normetanephrine;metanephrine;3-methoxytyramine;spot urine;PPGLs diagnosis
研究领域:
医学
·
生物化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xiaomin Cao,Miao Qin,Pan Li,Binbin Zhou,Xianghu Tang,Meihong Ge,Liangbao Yang,Jinhuai Liu
Catecholamine (CA) is neurotransmitters of the biological amines class which play an important role in organism. However, it is quite difficult to realize sensitive and selective detection of CA in complex system. Here, we employ surface-enhanced resonance Raman spectroscopy (SERRS) strategy, iron-nitrilotriacetic acid functionalized PVP-Au NPs (Au-Fe(NTA)) as Raman label for rapid and sensitive detection of CA containing dopamine (DA), norepinephrine (NE) or epinephrine (EP) in complex serum. The Au NPs is sufficient to provide Raman enhancement and Fe-NTA label can rapidly trap CA molecules adjoining gold core to form NTA-Fe-CA resonant structure, which can amplify the signals of CA. More important, we successfully distinguish these three CA molecules in serum since surface-enhanced Raman spectroscopy (SERS) technique can provide fingerprint identification. Furthermore, the SERS signals of Au-O band from PVP stabilized Au NPs can be utilized as a stable internal calibration standard for quantitative detection of target. Additionally, we continue to investigate the binding constants between different CA molecules and functionalized substrate to evaluate the corresponding adsorption property. This SERRS strategy is not only capable to offer exciting opportunities to selectively trap the analyte, but also strongly amplify the Raman signals of CA as well as achieve quantitative measurement.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2014
·Vol. 37
·期 16
·2239-2247
·
IF 2.594
Nengsheng Ye,Jian Li
A novel capillary electrochromatography method was developed for the determination of dopamine (DA), epinephrine (EP), and norepinephrine (NE) by using a graphene oxide (GO) molecularly imprinted polymers (MIPs) coated capillary. In this article, GO was introduced as supporting matrix to synthesize MIPs in the presence of DA as template molecule. Then GO MIPs were used as the stationary phase in electrochromatography for the determination of DA, EP, and NE. The separation of these three analytes was achieved under the optimal conditions with a satisfactory correlation coefficients ( R 2 ) > 0.9957 in the range of 5.0–200.0 μg/mL for EP and NE, and 20.0–200.0 μg/mL for DA, respectively. The RSDs for the determination of three analytes were <6.19%, and the detection limits were 1.25 μg/mL for EP and NE, and 10.0 μg/mL for DA, respectively. Finally, this method was used for the determination of DA, EP, and NE in human serum and DA hydrochloride injection.
研究领域:
分析化学
·
药物分析
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 16
·6785-6790
·
IF 1.821
Shuyun Zhu,Jing Yang,Xian-en Zhao,Rongmei Kong,Hua Wang,Jinmao You
Catecholamines, generally including dopamine (DA), epinephrine (EP), and norepinephrine (NE), are important neurotransmitters and serve many central nervous system functions. In this work, a simple colorimetric method for the determination of the three catecholamines is developed. The proposed method is based on the fact that Ag+ could oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB) and induce a blue color solution corresponding to an absorption peak centered at 652 nm. However, the introduction of catecholamines could cause the reduction of oxTMB which results in the fading of the blue color quickly and a decrease of the absorbance at 652 nm. Based on this finding, we propose a method to quantitatively detect catecholamines with the help of UV-vis spectroscopy. The detection limit (S/N = 3) for DA, EP, and NE is 50 nM, 100 nM, and 150 nM, respectively. More importantly, this method is simple, fast and without the use of complicated nanomaterials. This method has been successfully applied to the detection of catecholamines in pharmaceutical samples.
研究领域:
分析化学
·
药学
·
神经科学
✓ 已验证
产品关联
DOI
高影响因子
ANNALS OF CLINICAL AND LABORATORY SCIENCE
·
2019
·Vol. 49
·期 2
·204-211
·
IF 0.96
Xue Xue,Hucheng Chen,Qingqing Xia,Ping He,Yuan Lv,Peng Liu,Yan Yang,Chaochao Wu,Qiang Gao,Jun Peng,Yang Yu,Feng Wang,Wei Qu,Zizheng Wang
Background The measurement of plasma catecholamines (CAs) including dopamine (DA), epinephrine (E), and norepinephrine (NE) and theirderivatives including metanephrine (MN), normetanephrine (NMN), vanillylmandelic acid (VMA), and homovanillic acid (HVA) hasbeen used in the diagnosis of pheochromocytoma and paraganglioma (PPGL) and primary hypertension (PH) but are typically detectedindividually when clinical testing. In this study, pre-column derivatization with dansyl chloride (DNS-Cl) combined with anultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to simultaneously quantifyHVA, VMA, MN, NMN, DA, E, and NE in the plasma from patients with PPGL and PH. Methods Plasma samples were extracted by acetonitrile and derivatized with DNS-Cl, followed by reverse phase separation and triplequadruple detection. Quantification of the CAs and their derivatives in 10 PPGL, 10 PH, and 100 healthy subjects was performedby UPLC-MS/MS analysis. Results All the values of detected CAs/derivatives were in the linearity ranges of the fitted curves. The expression levels of theseven CAs in the PPGL and PH patients were significantly higher than the healthy controls, suggesting increased CA productionin the former. There were significant differences in plasma NE, NMN, and VMA levels between the PPGL and PH patients, butthere was no significant difference in plasma E, MN, DA, and HVA. A discriminant analysis showed that 90% of the final caseswere classified correctly based on the detected CAs/derivatives. Conclusions Our results show that the combined detection of the seven CAs/derivatives could be used for the clinical diagnosis of PPGLand PH.
研究领域:
临床化学
·
内分泌学
·
药理学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2019
·
IF 3.17
Qi-meng Liu,Qing Xiao,Xiang Zhu,Kai-feng Chen,Xia-wen Liu,Ru-chao Jiang,Dan Wu,Jun-min Shi,Li-jun Dai,Jun-jun Huang
Benign prostatic hyperplasia (BPH) is a common disorder of the urinary system in aging men. 2-(1H-indol-3-yl)-N-[3-(4-(2-methoxyphenyl) piperazinyl) propyl] acetamide (HJZ-3), which is derived from naftopidil , exhibited 97.7- and 64.6-fold greater inhibitory effects for a 1D adrenoceptor than for a 1B - and a 1A -adrenoceptors in vitro, respectively. To investigate the therapeutic potential for treating BPH, we evaluated the pharmacological activity of HJZ-3. Specifically, we evaluated through estrogen/androgen-induced rat benign prostatic hyperplasia model in vivo. HJZ-3 effectively prevented the progression of rat prostatic hyperplasia by suppressing the increase in prostate index and reducing the quantitative analysis of the relative acinus volume, relative stroma, epithelial volume and epithelial thickness and expression of proliferating cell nuclear antigen and α -smooth muscle actin. HJZ-3 decreased α 1A - and α 1D -adrenoceptor protein expressions in prostate tissue. HJZ-3 is a good alternative for α 1A - and α 1D -adrenoceptor blocker. It may relax smooth muscle tone and relieve symptoms of BPH.
研究领域:
药理学
·
泌尿外科
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）