Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Zechun Dang,Jizheng Sun,Jiaqi Fan,Jinqi Li,Xinlei Li,Tongsheng Chen
Zinc oxide (ZnO) nanostructures have been widely studied in biomedical fields due to their special properties. In recent years, ZnO spherical nanoparticles (SNPs) with nano-size as an anti-tumor agent have been widely concerned. While the effects of the non-spherical shaped ZnO nanoparticles on tumor cell death have been rarely reported. Here, we prepared ZnO spiky nanoparticles (SPNPs) as the research subject. We found that the SPNPs showed superiority in killing tumor cells. To be specific, SPNPs presented a long-term cytotoxicity effect on killing tumor cells, as plenty of SPNPs retained on the cell plasma membrane's exterior and still showed toxicity effect on tumor cells after co-incubation multiple times. Moreover, compared to SNPs, it was encouraging that SPNPs still showed stronger cytotoxicity in both simulated circulatory systems of tumor cells and 3D tumor cell spheroids. The stronger toxicity against tumor cells suggested that ZnO SPNPs have more advantages on killing tumor cells as a promising nanomedicine.
研究领域:
生物医学工程
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纳米技术
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Yanan Xu,Hui Wu,Chusen Huang,Caiqin Hao,Beina Wu,Chongchong Miao,Shen Chen,Nengqin Jia
Vicinal-dithiol-containing Proteins (VDPs) are overexpressed in cancer cells and become a potential biomarker for aggressive tumors, the synthesized 2-p-aminophenyl-1, 3, 2-dithiarsenolane (VTA2) is proved to be a highly selective ligand for vicinal dithiols of VDPs in cells. In this report, we developed a new cytosensor based on three-dimensional (3D)-like VTA2-conjugated multiwalled carbon nanotubes (VTA2@MWCNTs) array modified indium tin oxide (ITO) electrode for sensitive and selective detection of VDPs-overexpression tumor cells. The layer-by-layer assembling and cellular detection performances of the 3 D -VTA2@MWCNTs-based cytosensor were investigated by atomic force microscopy (AFM) and electrochemical methods including cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). Attributed to signal amplification and targeting recognition of the 3 D -structured MWCNTs@VTA2, the fabricated cytosensor showed high specificity and sensitivity to the detection of VDPs-overexpression HL-60 cells ranging from 2.7×10 2 to 2.7×10 7 cells mL −1 with a low detection limit of ca. 90 cells mL −1 . Furthermore, the captured cancer cells on the cytosensor also could be directly visualized by optical microscopy technology. This proposed 3 D -MWCNTs array-based cytosensing strategy provides a simple, sensitive approach for non-invasive cell detection, presenting potential applications in cancer diagnosis.
研究领域:
生物传感器技术
·
癌症诊断
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纳米材料应用
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产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2021
·
IF 7.991
Long Pang,Haohui Feng,Wei Zhong,Haonan Dong,Youqing Shen,Bing Yu,Hailin Cong
Chemotherapy is one of the most used method for cancer therapy, however, brings huge pain to patients as the drugs commonly causes side effects. Inducing tumor cells apoptosis by perturbing ion homeostasis is a novel approach to replace chemotherapy in recent years. Crown ether has been widely used since it was discovered in the last century and has shown its potential in biological applications. The aim of this paper is to design a series of novel nanometer micelles with crown ether on their surface. Crown ether was connected to a commonly used amphiphilic polymeric carrier, PEG-PCL, through ester bond (CPMs). Micelles about 120 nm in diameter were formed in aqueous solution. The micelles could capture potassium ions prominently in both aqueous solution and tumor cells. In vitro and in vivo experiments showed that the CPMs had certain anti-tumor activity, indicating that the CPMs had anti-tumor potential.
研究领域:
药学
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生物化学
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肿瘤学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 9
·1712-1723
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IF 4.543
Rui He,Mingxian Liu,Yan Shen,Zheru Long,Changren Zhou
Here, polystyrene sulfonate sodium (PSS) modified Halloysite nanotubes (HNTs) were self-assembled into a patterned coating on a glass substrate with ordered nanotube arrays in a slit-like confined space. The microstructure of the formed patterned HNTs coating was investigated. The formed strips are more regular and almost parallel to each other with an increase in HNTs concentration. The HNTs coating formed from the 2% PSS-HNTs dispersion has the maximum nanotube alignment degree. The patterned HNTs coating was employed to capture tumor cells. The tumor cells can be captured by the HNTs coating effectively compared with a smooth glass surface due to the enhanced topographic interactions between the HNTs coating and cancer cells. The HNTs coating prepared from the 2% PSS-HNTs dispersion has the highest capture yield which is due to the ordered nanotube arrangement and the appropriate surface roughness. The HNTs coating was further conjugated with anti-EpCAM, which leads to the capture yield of MCF-7 cells reaching 92% within 3 h. The HNTs coating can capture 8 MCF-7 cells from 1 mL artificial blood samples spiked with 10 MCF-7 cells, showing the promising applications of HNTs in clinical circulating tumor cell capture for early diagnosis and monitoring of cancer patients.
研究领域:
纳米技术
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生物材料
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癌症研究
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2013
·
IF 5.008
Hui Zhang,Ronghua Shi,Anjian Xie,Juchuan Li,Long Chen,Ping Chen,Shikuo Li,Fangzhi Huang,Yuhua Shen
A novel inorganic/organic hybrid hydrogel system containing titanium dioxide (TiO2)/poly(ethylene glycol) double acrylates (PEGDA) was prepared by in situ photopolymerization on tumor cells for photodynamic therapy (PDT). TiO2 nanorods with diameter of ∼5 nm and length of ∼25 nm in this system presented dual functions, as effective photosensitizers for PDT and initiators for causing the in situ formation of hydrogel, under near-infrared (NIR) irradiation. The hybrid hydrogel retained the TiO2 around tumor cell to form a drug-loaded hydrogel shell. This resulted in a high concentration of singlet oxygen (1O2) under NIR irradiation, which induced apoptosis of tumor cell. Also, the hydrogel could reduce the side effects by preventing TiO2 from migrating to normal tissue. Furthermore, the TiO2 nanorods in this hydrogel shell were photochemically recyclable and could be reused in regular treatment. The outcomes of this study provide a new way to exploit multifunction of inorganic semiconductor nanomaterials for a variety of biomedical applications.
研究领域:
生物医学工程
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纳米医学
·
癌症治疗
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Zhengying Peng,Youyuan Chen,Pengkun Yin,Fan He,Daibing Luo,Yixiang Duan,Qingwen Fan,Zhimei Wei,Qingyu Lin
Circulating tumor cells (CTCs) are cancer biomarkers for the diagnosis at early stage. However, it’s still a great challenge to develop the cytosensor for CTCs detection due to its ultralow counts in human peripheral blood. Herein, a highly sensitive cytosensor based on surface-enhanced Raman scattering (SERS) was developed. The tumor cells were first identified by a strand replacement reaction (SDR) that released the initiator of catalytic hairpin assembly (CHA) reaction. Subsequently, the initiator opened the residue hairpin DNA outside the silver coated gold nanoparticles ( [email protected] NPs). The double strand DNA (dsDNA) CHA products drove the [email protected] NPs towards the same side of dsDNA to form a zipper-like structure. This structure brought about hotspots area for SERS quantitative analysis of A549 tumor cells. The designed cytosensor exhibited good linearity ranging from 10 to 500 cells/mL with a calculated limit of detection (LOD) of 8 cells/mL. A SERS cytosensor with high sensitivity and good reproducibility was obtained in this work. In addition, this research presents a new method for rapid tumor cell detection in biological samples for cancer diagnosis.
研究领域:
生物传感器
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癌症诊断
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分子生物学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Macromolecular Science Part A-Pure and Applied Chemistry
·
2019
·
IF 1.163
Jie Wang,Songyan Liu,Ying Chang,Le Fang,Kai Han,Miao Li
Nowadays development of safe and simple siRNA delivery system is still a great challenge. Carbon dots have attracted considerable attention in bioimaging, drug delivery, nanosensors, and other fields during the past few years. In this study, we successfully developed carbon dots (TEPA-CDs) as siRNA nanocarriers based on glucose and tetraethylene pentamine. TEPA-CDs have a mean diameter of less than 10 nm and positive charged decoration. TEPA-CDs could condense siRNA into stable complexes without no obvious premature release. Cellular uptake analysis clearly showed that Cy3-labeled siRNA could be uptake by HeLa cells. GFP expression in HeLa-EGFP cells could be significantly inhibited by TEPA-CDs/siRNA complexes. Our study indicated that TEPA-CDs could be used as a siRNA nanocarrier to tumor cells.
研究领域:
纳米技术
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分子生物学
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癌症研究
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药物递送
·
生物成像
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Rong Qin,Mengqi Bai,Feiyi Zhang,Zengkai Wang,Fenglian Liu,Mingdong Dong,Lei Liu,Jian Xu
The detection of circulating tumor cells (CTCs) has emerged as a promising approach for the early diagnosis, therapeutic monitoring, and prognosis of malignant tumors. In this study, we present a novel magnetic nanoparticle (MP) functionalized with dual recognition peptides targeting the epithelial cell adhesion molecule (EpCAM) and human epidermal growth factor receptor 2 (HER2). Additionally, bovine serum albumin (BSA), an anti-fouling agent, is employed onto MP, effectively preventing the non-specific adhesion of platelets and proteins. This multifunctional peptide based magnetic biointerface (MPMB) platform effectively captures breast cancer cells in model systems and isolates CTCs from the blood of breast cancer patients. CTC enumeration of peripheral blood samples from 40 breast cancer patients, 30 benign breast tumor patients, and 30 healthy donors revealed a 70 % positivity rate in breast cancer patients (28/40), 6.7 % in benign breast tumor patients (2/30), and no positivity in healthy donors (0/30). CTC counts were significantly higher in breast cancer patients with advanced stages, particularly in those with distant metastasis, compared to early-stage patients ( p < 0.05). Moreover, among 10 breast cancer patients with positive CTC detection prior to surgery, 6 showed negative CTC results post-surgery. These findings indicate that the MPMB approach provides a simple, flexible, and efficient platform for monitoring therapeutic efficacy and evaluating prognosis in breast cancer patients undergoing treatment.
研究领域:
医学
·
生物技术
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产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Rui He,Mingxian Liu,Yan Shen,Rong Liang,Wei Liu,Changren Zhou
Here, we reported a fast, low-cost, and effective fabrication method of large-area and rough halloysite nanotubes (HNTs) coatings by thermal spraying of HNTs ethanol dispersions. A uniform HNTs coating with high transparence is achieved with tailorable surface roughness and thickness. Compared with normal cells, the tumor cells can be captured effectively with high capture yield by the HNTs coatings (expect HeLa cells), which is attributed to the enhanced topographic interactions between HNTs coating and cancer cells. HNTs coating formed from 2.5% ethanol dispersions shows the highest tumor cells capture yeild (90%), which is related to the appropriate roughness and anti-EpCAM conjugation. The capture yield of HNTs coating towards MCF-7 cells can be further improved to 93% within 2 h under dynamic shear using a peristaltic pump. The capture yield increases with the incubation time, and the flow rate with 1.25 mL/min leads to the maximum capture yield. The HNTs coatings are also effective for capture of tumor cells spiked in artificial blood samples and blood samples from patients with metastatic breast cancer. More than 90% targeted MCF-7 cells and very small amounts of white blood cells are captured by the anti-EpCAM conjugated HNTs coatings from a blood sample. HNTs are further loaded anticancer drug doxorubicin (DOX) and then thermally sprayed into coatings. The MCF-7 cells captured on DOX loaded HNTs coating display significant membrane rupture characteristic and only 3% cell viability after 16 h. The high capture efficiency of tumor cells by HNTs coating fabricated by the thermal spraying method makes them show promising applications in clinical circulating tumor cells capture for early diagnosis and monitoring of cancer patients. The high killing ability of the DOX loaded HNTs coating can also be designed as an implantable therapeutic device for preventing tumor metastasis.
研究领域:
材料科学
·
生物医学工程
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癌症研究
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2014
·Vol. 14
·期 10
·1415-1428
·
IF 3.65
Xiaoyu Ai,Jin Sun,Lu Zhong,Chunnuan Wu,Handong Niu,Tao Xu,He Lian,Xiaopeng Han,Guolian Ren,Wenya Ding,Jia Wang,Xiaohui Pu,Zhonggui He
A redox-responsive poly(ethylene glycol) (PEG)-sheddable copolymer of disulfide-linked PEG 5000-lysine-di-tocopherol succinate (P 5k SSLV) is developed which can self-assemble into nanomicelles in aqueous condition and trigger the rapid release of encapsulated drugs within tumor cells. The reduction-insensitive doxorubicin (DOX)-loaded P 5k LV (P 5k LV-DOX) nanomicelles are further prepared. Then head-to-head comparison of P 5k SSLV-DOX, P 5k LV-DOX and DOX-Sol is performed concerning in vitro release, cytotoxicity, cellular uptake and apoptosis. Results show that P 5k SSLV-DOX nanomicelles have a faster DOX release, a higher anti-tumor activity and more DOX concentrating in the nucleus than P 5k LV-DOX nanomicelles. In conclusion, the redox-responsive P 5k SSLV nanomicelles might hold a great potential to improve chemotherapy by tumor-triggering intracellular rapid release. The outcomes of this study also address the significance of such head-to-head comparison studies in translational research of nanomedicine.
研究领域:
药剂学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
COLLOID AND POLYMER SCIENCE
·
2019
·Vol. 297
·期 2
·225-238
·
IF 1.906
Zhang Longshuai,Liu Yuancheng,Zhang Kui,Chen Yuanwei,Luo Xianglin
Redox-responsive block copolymer micelles have been studied extensively as drug carriers. In this study, tri-block copolymers, methoxyl poly(ethylene glycol)-b-poly(ε-caprolactone)-SeSe-b-poly(ε-caprolactone)-b-methoxyl-poly(ethylene glycol) (mPEG-PCL-SeSe-PCL-mPEG) and methoxyl poly(ethyleneglyco)-b-poly(ε-caprolactone)-SS-b- poly(ε-caprolactone)-b-methoxyl-poly(ethylene glycol) (mPEG-PCL-SS-PCL-mPEG), were synthesized to investigate the redox sensitivity differences between diselenide and disulfide micelles. The changes of micelles in size and structure were investigated under conditions with glutathione (GSH) or H 2 O 2 . The results showed that the size and PDI of (mPEG-PCL-Se) 2 micelles presented more significant variations under redox condition in comparison with (mPEG-PCL-S) 2 micelles. The DOX released faster and more from diselenide micelles than disulfide micelles. The half maximal inhibitory concentration (IC 50 ) of (mPEG-PCL-Se) 2 /DOX micelles was lower than that of (mPEG-PCL-S) 2 /DOX micelles against 4T1 and Hela cells. The amount of intracellular drug release from diselenide micelles was higher than from disulfide micelles in Hela cells with GSH 4.6 mM. Therefore, the (mPEG-PCL-Se) 2 micelles similar to (mPEG-PCL-S) 2 micelles are stimuli-responsive and may be more sensitive drug carriers.
The polymer micelles containing diselenide or disulfide with similar molecular structure are prepared to investigate the redox sensitivity differences. Both micelles have redox sensitivity and capability to enter into 4T1 cells and Hela cells. (mPEG-PCL-Se) 2 micelles present more significant sensitivity and lower IC 50 against cancer cells than disulfide micelles. The diselenide micelles may be more sensitive platforms and be suit to tumor cells in which GSH is less than 10 mM.
研究领域:
药学
·
生物化学
·
材料科学
✓ 已验证
DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2017
·Vol. 33
·期 4
·581-586
·
IF 1.024
Guo Jinhua,Tan Bei,Ye Qingmei,Liang Guangping,Yi Min,Jiang Renwang
Two series of novel spin-labeled derivatives of Cinobufagin(compounds 5 and 8 a― 8 f in series 1 with five-membered ring nitroxyl free radical and compounds 6 and 9 a― 9 f in series 2 with six-membered ring nitroxyl free radical) were synthesized. The cytotoxic activities in vitro against two tumor cell lines(HepG2 and HeLa) were evaluated, and the results indicate that all compounds display potent cytotoxicity against HepG2 and HeLa cells, and most compounds show better activities on HeLa cells than on HepG2 cells except for compounds 8 a and 9 d. Generally, the compounds in series 2 have more potent cytotoxic activity against HepG2 than the compounds in series 1. Especially, compounds 6 and 9 f in series 2 exhibit even more potent activities against the two tumor cell lines than Cinobufagin. Thus incorporation of different L -amino acids as the linker changed the cytotoxic profile of the spin-labeled Cinobufagin. In addition, the representative compound 9 f significantly changed the cell cycle distribution and led to HeLa cell cycle arrested at G 2 /M phase.
研究领域:
药物化学
·
肿瘤学
·
分子生物学
·
药理学
·
有机合成
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）