JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2022
·
IF 5.062
Lanlan Lin,Haiyang Li,Zekun Chen,Xinyu Wen,Rui Yan,Lei Liu,Caihong Tao
The combination of chemotherapy and photothermal therapy can provide better tumor treatment effect than monotherapy , while the combination of imaging technology can achieve diagnostic effect during the treatment. In this paper, biocompatible mesoporous polydopamine (MPDA) were combined with the near-infrared fluorescent dye IR780 under alkaline condition to form an integrated drug delivery platform for tumor diagnosis and photothermal co-therapy. The particles size and mesoporous pore size of the nanospheres were adjusted by changing the preparation conditions of MPDA, so the platform had strong permeability and large drug loading capacity in tumor cells as drug carriers. The loading and release results of doxorubicin hydrochloride (DOX) proved that the carrier had a high drug loading and sustained release rate, as well as a faster dependent release under acidic and lighting conditions. Photothermal experiments showed that the carrier had high photothermal conversion efficiency, which was very critical in its application in photothermal therapy and fluorescence imaging of tumors. The vitro cytotoxicity test evaluated the survival rates of LX-2 cells and HepG2 cells after drug loading. The results showed that the MPDA@IR780-DOX + NIR carrier had a significant inhibitory effect on HepG2 cells (12.57%), but had little effect on LX-2 cells, which was beneficial for its application in integration of biological diagnosis and treatment.
研究领域:
生物医学工程
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纳米医学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2024
·
IF 2.5
Di Hua,Hongjie Xi,Quan Xie,Shuyue Cai,Yuxuan Zhou,Xin Hu,Ling Qiu,Jianguo Lin
Legumain is overexpressed in diverse tumors, serving as a significant tumor biomarker. Our study aimed to develop a new positron emission tomography (PET) probe [ 68 Ga]Ga-NOTA-SF-AANM for imaging the expression level of legumain in vivo . The radio-labeling of [ 68 Ga]Ga-NOTA-SF-AANM was accomplished within 15 min. The probe has good stability in vitro . NOTA-SF-AANM exhibited rapid response to recombinant human legumain enzyme, enabling intramolecular condensation cyclization. Cellular uptake and lysosomal co-localization experiments demonstrated that the probe was able to differentiate specifically between MDA-MB-468 and PC-3 cancer cells with varying degrees of legumain expression. PET imaging displayed a significant and persistent signal (3.59 ± 0.30 %ID/mL at 60 min) in MDA-MB-468 tumors, while PC-3 tumors exhibited lower radioactivity (1.08 ± 0.35 %ID/mL at 60 min), further validating the specific targeting of [ 68 Ga]Ga-NOTA-SF-AANM towards legumain. [ 68 Ga]Ga-NOTA-SF-AANM is a promising tool for precise diagnosis of legumain-related diseases due to its advantages in radio-labeling and accurate monitoring of legumain expression levels.
研究领域:
医学影像学
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分子生物学
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肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
Science of Advanced Materials
·
2021
·Vol. 13
·期 7
·1364-1373
·
IF 1.474
Zhang Yumei,Pei Ruina,Luo Tao
ABSTRACT
The development of nanomaterials, especially the development of multi-modality imaging nanoprobe technology has become a reality for tumor imaging diagnosis. In this study, the gadolinium ion chelating agent (2,2′,2′′-(10-(2-(2, 5-dioxopyrrolidin-1-oxyl)-2
oxoethyl)-1, 4, 7, 10-tetraazacyclododecane-1, 4, 7-tri)triacetic acid) (DOTA-NHS) and polyethylene glycol (PEG)-modified targeting ligand PEG-folic acid (FA) were grafted onto the PEG-modified fifth-generation polyamide-amine dendrimer (P5-NH2), which were undertaken as standards
to wrap gold nanoparticles. Then, remaining amino group was acetylated to acquire Gd–Au DENPS-FA nanoparticles. Biocompatibility and cytotoxicity of nanoparticles were analyzed while characterizing them. The cases with unidentified tumors (mass diameter >4.5 cm) of abdominal or pelvic
origin were selected and used for clinical study of computed tomography (CT)/magnetic resonance imaging (MRI) dual-modality abdominal tumors. In the test, average particle size of Gd-Au DENPs-FA nanoparticles was 4.2 nm, and they had good water solubility and stability in aqueous solution.
When concentration of Au+ nanoparticles increased, the cell morphology remained normal, only a small number of round cells appeared, and the cell activity remained above 80%. GD-Au DENPs-FA nanoparticles had a good targeting specificity for KB cells with high folate receptor expression.
During 6–24 hours of injection, the signal at the tumor site of the patient was enhanced markedly, namely the targeted gold nanoparticles were bound to the tumor tissue and the metabolic rate was slow. Based on this material, CT/MRI imaging could be performed in the patient’s body
and further used for the early diagnosis of abdominal tumors.
研究领域:
医学影像学
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纳米生物学
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肿瘤学
✓ 已验证
DOI
高影响因子
INTEGRATED FERROELECTRICS
·
2023
·
IF 0.7
Lu-Ping Chen,Zhen Zhang,Wang-Quan Zhang,Hai-Lin Cong,You-Qing Shen,Bing Yu
Malignant tumor is one of the most important causes of death in the world today. At present, the successful cure cases of tumor patients are mainly due to the timely treatment of tumors. Therefore, it has become one of the urgent problems for human beings to find tumors as soon as possible and how to completely eradicate them. The technology of simultaneous imaging and treatment through photoinitiation is a promising strategy to fight cancer. In the previous research, we developed a new small molecule p-QM2AF for photothermal therapy, but the low fluorescence efficiency (QY) in the NIR-II range made it necessary to modify it. In this paper, we obtained better imaging and photothermal conversion performance by grafting two 9- methylene nonadecanes onto the p-QM2AF sub-receptor.
研究领域:
生物医学工程
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肿瘤学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Science China-Chemistry
·
2018
·Vol. 62
·期 1
·105-117
·
IF 4.448
Ma Jinlong,Li Xiaomin,Hu Zhenpeng,Wang Xinyu,Zhang Yapei,Wang Wei,Wu Qiang,Yuan Zhi
Due to the ability to combine the separately unique characteristics of assembled and disassembled nanoparticles (NPs), the stimuli-responsive self-assembly of NPs has attracted considerable interest in functional material applications especially biomaterials. Here we demonstrate a facile and versatile approach to regulate the self-assembly process and transition pH of Au NPs by fine-tuning the co-modified pH-responsive compounds and poly(ethylene glycol) (PEG). Importantly the transition pH (ΔpH=0.4) of the system can be predetermined in the range of 8.2–5.8 (assembled to disassembled) and 8.2–4.2 (disassembled to assembled), which ideally covers the pH of normal tissue, tumor tissue milieu and organelles. The results of fluorescence imaging, Raman spectroscopy and photothermal conversion of the stimuli-responsive Au NPs shows the potential application for tumor specificity theranostics. In a nutshell this study provides a useful toolkit to design tumor-activatable self-assembled NPs with high specificity and universality.
研究领域:
生物材料
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纳米医学
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肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
Nano Research
·
2018
·Vol. 11
·期 8
·4333-4347
·
IF 7.994
Li Niannian,Cheng Jiejun,Zhang Ying,Wang Jie,Huang Gang,Zhu Jun,He Dannong
The development of multifunctional nanoparticles for diagnosis and therapy of cancer has been a focus of research in recent years. Owing to excellent properties of chemophotothermal therapy and tumor targeting accompanied by magnetic resonance (MR) or computed tomography (CT) imaging, mesoporous silica has been widely applied to tumor diagnosing carriers. However, previous research focused more on chemotherapy combined with photothermal therapeutic strategies but ignored intuitive assessment of tumors. In this work, we report a novel nanoprobe consisting of hyaluronic-acid–modified Gold Nanorods@mSiO2@Mn@DOX (GNR@MMH@DOX) nanoparticles, which realize multifunctional integration of therapeutics and imaging. Au nanorods (GNRs) were synthesized without a seed precursor by a water bath method, then sequentially modified with mesoporous silica, doped with Mn 2+ , and loaded with doxorubicin in a solution. In vitro and in vivo assays verified their great biocompatibility, good stability, and attractive specificity of targeting to receptor CD44, which is overexpressed on cancer cells. In addition, GNR@MMH nanoparticles proved to be a more distinguishable CT/MR imaging contrast compared to commercial contrast agents. The article demonstrates promising composition for CT/MR imaging and photothermal therapy of tumors, giving new insights into the diagnosis and therapy of different kinds of malignant tumors.
研究领域:
生物医学工程
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肿瘤学
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纳米医学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Xiaojing Yang,Yingying Wang,Jingjing Xu,Mei-Xia Zhao
In recent years, carbon dots (CDs) have shown potential applications in fluorescence tracing and imaging due to their excellent optical and chemical properties. In this work, a new type of carbon dots (Cy7-CDs) with near-infrared absorption and emission was synthesized from cyanine dyes and polyethylene glycol by hydrothermal reaction. The prepared Cy7-CDs exhibited low toxicity and excellent biocompatibility, which were applied to detect the noble metal ions Au 3+ /Hg 2+ and glutathione via the ‘on–off-on’ mode. In addition, in vivo fluorescence images demonstrated that Cy7-CDs could efficiently accumulate in the tumor. Therefore, Cy7-CDs could act as a multi-functional probe for biological sensing and tumor diagnosis.
研究领域:
生物传感
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医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Feichi Hu,Jiying Xu,Yi Chen
MicroRNA (miRNA) is a promising new type of biomarkers but at a low fM level and hard to be analyzed. Herein proposed is an innovated surface plasmon resonance imaging (SPRi) method merged with a novel in-plane and vertical signal amplification strategy, that is, orthogonal signal amplification to enable a direct determination of sub-fM miRNA-15a (a multiple tumor diagnostic biomarker). The core idea is to add more mass on a target sample spot first along the surficial direction, then upward from the surface. In detection of miRNA, this was realized by coupling a miRNA-initiated surficial cyclic DNA–DNA hybridization reaction with a DNA-initiated upward cyclic polymerization reaction. A perfect SPRi sensing chip with isolated gold islands bordered by hydrophobic CYTOP was fabricated and used to obtain high-quality chip with low fabrication difficulty. As a result, SPRi contrast largely increases, able to reach a limit of detection and limit of quantification down to 0.56 and 5fM for miRNA-15a, about 107-fold improvement of sensitivity compared with a common SPRi detection. The method could quantify standard miRNA-15a spiked in human serum with an ideal recovery ranging from 98.6% to 104.9% and was validated to be applicable to the direct determination of miRNA-15a in healthy and cancer human serums. The orderly and controllable in situ sensitizing strategy is powerful and readily extendable to detection of other miRNAs.
研究领域:
生物化学
·
分子生物学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2018
·
IF 4.37
Dan Wang,Yuwen Zhang,Qi Guo
Background Facile synthesis and small size theranostic agents have shown great potential for cancer diagnosis and treatment.Purpose A ternary compound (Cu5FeS4), Fe doped copper sulfide, with novel magnetic properties and strong near-infrared absorption was prepared for magnetic resonance imaging (MRI) imaging guided photothermal therapy of cancer.Patients and methods Firstly, the capability of magnetic resonance imaging based on the novel magnetic properties and the photothermal performance due to the strong near-infrared absorption was investigated in vitro. Then, the magnetic resonance imaging guided photothermal therapy for 4T1 tumor-bearing mouse was carried out.Results The Cu5FeS4 cube with good T1-weighted MRI, excellent photothermal performance and low cytotoxicity has been investigated. More importantly, the T1-weighted MRI for 4T1 tumor-bearing mouse will get the best contrast effect at tumor site after 8 h of intravenous injection of Cu5FeS4 cube. Under the guidance of the T1-weighted MRI, the PTT was carried out at 8 h after intravenous injection of Cu5FeS4 cube and only the group combined intravenous administration of Cu5FeS4 cube and laser irradiation nearly cured the tumor after 14 days.Conclusion Our study not only provides a new material for personalized treatment of tumors, but also further promotes potential applications of the cancer theranostic agents.
研究领域:
生物医学工程
·
癌症治疗
·
纳米医学
✓ 已验证
DOI
高影响因子
MOLECULAR MEDICINE
·
2025
·
IF 6.4
Quanpeng Qiu,Yi Ding,Xiaolong Guo,Jing Han,Jiaqi Zhang,Yaping Liu,Junjun She,Yinnan Chen
Background Extrachromosomal circular DNA (eccDNA) has potential in tumor diagnosis, particularly for improving diagnostic accuracy and early cancer detection; however, many challenges remain in its application to clinical practice. Methods We conducted a Circle‐Seq analysis on clinical samples at different stages of colorectal cancer progression to examine the dynamic changes of eccDNA during the progression of colorectal cancer. We used breakpoint-specific PCR to verify candidate eccDNAs identified by Circle‐Seq. The results were further validated using the AOM/DSS-induced colorectal cancer model. Results There was an increase in the abundance of eccDNA with the progression of colorectal cancer. The genes associated with these eccDNA molecules were primarily related to signaling pathways involved in tumor development and metastasis. Our analysis also revealed that eccDNA abundance positively correlates with gene expression, and eccDNA derived from specific genes has potential value for the early diagnosis of tumors. Conclusions This study revealed a connection between eccDNA and colorectal cancer progression and highlights the clinical potential of eccDNA for the early diagnosis of colorectal cancer. Supplementary Information The online version contains supplementary material available at 10.1186/s10020-025-01164-y.
研究领域:
医学
·
分子生物学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Yiqi Zeng,Siyu Zhou,Yicheng Yang,Bin Tang,Weijun Wei,Gang Huang,Changfeng Wu,Xiaofeng Fang
Nanobodies have gained significant attention as promising tools for cancer diagnostics and treatment due to their unique ability to precisely target specific cancer cells. However, a major challenge lies in the site-specific incorporation of multifunctional molecules into nanobodies, as it is essential to link these molecules in a manner that preserves the nanobody’s function and stability while retaining the desired therapeutic or diagnostic properties. This study outlines the development of dual-functional nanobody optical probes for enhanced cancer diagnostics and therapeutic interventions. We designed a dual-functional clickable linker that enables site-specific functionalization of the nanobody, facilitating the simultaneous conjugation of two dyes: indocyanine green for imaging and chlorin e6 for photodynamic therapy. In vitro cellular assays confirmed the successful labeling of the dual-functional dyes, with the nanobody probe exhibiting high cellular binding specificity. In vivo imaging of mice bearing Hep3B tumors revealed clear visualization with a high signal-to-noise ratio. Furthermore, PEGylated probes significantly improved tumor retention, enhancing both imaging contrast and photodynamic therapy efficacy as compared to free chlorin e6. These dual-functional nanobody probes show great promise for the precise diagnosis and treatment of malignant tumors.
研究领域:
生物医学工程
·
肿瘤学
·
分子影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Pharmaceutics
·
2024
·Vol. 16
·期 5
·655
·
IF 4.9
Meng Li,Aoxiang Luo,Wei Xu,Haoze Wang,Yuanyuan Qiu,Zeyu Xiao,Kai Cui
A visual Raman nano-delivery system (NS) is a widely used technique for the visualization and diagnosis of tumors and various biological processes. Thiophene-based organic polymers exhibit excellent biocompatibility, making them promising candidates for development as a visual Raman NS. However, materials based on thiophene face limitations due to their absorption spectra not matching with NIR (near-infrared) excitation light, which makes it difficult to achieve enhanced Raman properties and also introduces potential fluorescence interference. In this study, we introduce a donor–acceptor (D-A)-structured thiophene-based polymer, PBDB-T. Due to the D-A molecular modulation, PBDB-T exhibits a narrow bandgap of Eg= 2.63 eV and a red-shifted absorption spectrum, with the absorption edge extending into the NIR region. Upon optimal excitation with 785 nm light, it achieves ultra-strong pre-resonant Raman enhancement while avoiding fluorescence interference. As an intrinsically sensitive visual Raman NS for in vivo imaging, the PBDB-T NS enables the diagnosis of microtumor regions with dimensions of 0.5 mm × 0.9 mm, and also successfully diagnoses deeper tumor tissues, with an in vivo circulation half-life of 14.5 h. This research unveils the potential application of PBDB-T as a NIR excited visual Raman NS for microtumor diagnosis, introducing a new platform for the advancement of “Visualized Drug Delivery Systems”. Moreover, the aforementioned platform enables the development of a more diverse range of targeted visual drug delivery methods, which can be tailored to specific regions.
研究领域:
生物医学工程
·
材料科学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）