FOOD CHEMISTRY
·
2025
·
IF 9.8
Jie Yang,Yujing Sun,Xuyan Dong,Man Li,Yang Qin,Lei Dai,Qingjie Sun
This study investigates the interaction of amino-modified starch nanoparticles (NH 2 -SNPs) and unmodified SNPs with pepsin and trypsin and the influence of the formation of protein coronas on the release of polyphenols. We discovered that NH 2 -SNPs bound loosely to pepsin, while they bound tightly to trypsin, by quartz crystal microbalance with dissipation monitoring and zeta potential measurement. SNPs did not easily bind to the two digestive enzymes. In addition, the influence of NH 2 -SNPs on digestive enzymes was investigated by ultraviolet-visible spectrophotometry, and circular dichroism spectroscopy, showing that the addition of NH 2 -SNPs had no effect on the conformational structure of pepsin and trypsin. Using NH 2 -SNPs and SNPs to load four polyphenols revealed that the nanoparticles had a slow-release effect on the polyphenols, but the presence of protein coronas had little effect on the release. The release was mainly related to the destruction of the starch-based carrier by the amylase in digestive enzymes.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Yihui Wang,Yujing Sun,Jie Yang,Lei Dai,Na Ji,Liu Xiong,Qingjie Sun
Nanoparticles (NPs) can form a protein corona (PC) with proteins in biological fluids. We examined whether starch nanoparticles (SNPs) form a PC and interact with digestive enzymes in simulated gastric and intestinal fluids. We investigated the adsorption of pepsin and trypsin on unmodified, carboxyl-, and amino-modified SNPs (SNPs, COOH-SNPs, and NH2-SNPs, respectively). Quartz crystal microbalance data showed that a tight and irreversible pepsin corona formed on the NH2-SNPs, pepsin had little or no binding to the SNPs and COOH-SNPs, and trypsin had weak binding to all three kinds of NPs. Dynamic light scattering data showed that pepsin significantly increased the size of the NH2-SNPs from 120 ± 2.6 to 203 ± 12.2 nm and decreased their surface potential from 23.2 ± 1.0 to 12.7 ± 0.2 mV. NH2-SNPs could induce the fluorescence quenching of pepsin and change its secondary structures without affecting its activity.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Shaofeng Yuan,Changjian Li,Hang Yu,Yunfei Xie,Yahui Guo,Weirong Yao
Organophosphorus pesticides (OPPs) are a group of common residual pollutants in food that cause serious harm to human health. In the present study, the ability of 10 lactic acid bacteria (LAB) to degrade OPPs (dimethoate, parathion methyl, trichlorfon and chlorpyrifos) was explored for their potential application to degrade OPPs in fermented food . The results showed that some LAB can survive in the presence of OPPs and degrade them significantly in a short time. Among these, Lactiplantibacillus plantarum subsp. plantarum CICC20261 ( L. plantarum 20261) showed the highest degradation ability. The degradation behavior of OPPs by L. plantarum 20261 followed pseudo-zero-order kinetic model ( k = 1.0939–1.5915). Phosphatase produced by the strain played the key role in the degradation of OPPs because its activity was positively related to the degradation of OPPs ( r = 0.975–0.995). Phosphatase could degrade OPPs rapidly in vitro , further confirmed that L. plantarum 20261 degraded rather than adsorped the OPPs. In addition, the excellent antioxidant ability of LAB and the tolerance of simulated gastric and intestinal juices showed their potential protective effects against oxidative damage induced by pesticides in vivo .
研究领域:
食品科学
·
微生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Kefu Wang,Kangkang Wang,Yaoyao Chen,Siqi Liang,Yi Zhang,Changyan Guo,Wei Wang,Jide Wang
Microplastics (MPs) can enrich pollutants after being released into the environment, and the contaminants-loaded MPs are usually ingested by organisms, resulting in a potential dual biotoxic effect. In this paper, the adsorption behavior of Sulfamethoxazole (SMX) on Polyamide 6 (PA6) MPs was systematically investigated and simulated by the kinetic and isotherm models. The effect of environmental conditions (pH, salinity) on the adsorption process was studied, and the desorption behavior of SMX-loaded PA6 MPs was focused on simulating the seawater, ultrapure water, gastric and intestinal fluids. We found that lower pH and solubilization of SMX by gastrointestinal components (bovine serum albumin (BSA), sodium taurocholate (NaT), and pepsin) can reduce the electrostatic interaction between the surface charge of PA6 MPs and SMX. The result will lead to an increase in the desorption capacity of SMX-loaded PA6 MPs in gastrointestinal fluids and therefore will provide a reasonable mechanism for the desorption of SMX-loaded PA6 MPs in the gastrointestinal fluids. This study will provide a theoretical reference for studying the desorption behavior of SMX-loaded PA6 MPs under gastrointestinal conditions.
研究领域:
环境科学
·
生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Qihong Zhang,Hui Wang,Zongmiao Feng,Zhaohui Lu,Chen Su,Yunchun Zhao,Jingbo Yu,Alexandr V. Dushkin,Weike Su
As a functional polyphenolic compound, curcumin (CUR) has limited application due to its low solubility and bioavailability. In this work, curcumin loaded micelles self-assembled from disodium glycyrrhizin (Na 2 GA) was coated with pectin and tannic acid to construct a core-shell solid dispersion (SD-CUR). The assembly mechanism of the core-shell nanoparticle was clarified by molecular dynamics simulation. Improved solubility and in vitro release characteristics of SD-CUR in simulated gastric and intestinal juice were achieved. The parallel artificial membrane permeability (PAMPA) test confirmed that the permeability of curcumin in SD-CUR was significantly improved compared with the pure curcumin. Pharmacokinetic studies showed that the bioavailability was increased by 10 times. Intestinal in situ absorption study proved SD-CUR could enhance drug to transport across intestinal epithelial cells. Further hypolipidemic study demonstrated that the SD-CUR medication group could considerably reduce the levels of total cholesterol (TC), triglyceride (TG), and low density lipoprotein (LDL) in hyperlipidemia rats. These findings confirmed that SD-CUR had broad application prospects in the prevention and treatment of hyperlipidemia.
研究领域:
药剂学
·
分子动力学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Chunting Du,Tao Wang,Dandan Li,Xiaohu Luo,Jiali Xing,Ren Wang,Hao Zhang
Oral administration of insulin is still a big challenge due to the harsh gastrointestinal environment. In this study, starch and chitosan were utilized to prepare insulin-loaded microcapsules with controllable releasing properties. Herein, the complexation between chitosan and insulin upon pH adjustment tended to form positively charged insulin nanoparticles (iNPs). At a chitosan/insulin ratio of 1:2, the diameter of iNPs and corresponding insulin loading capacity reached 196.2 nm and 0.70 mg/mg iNPs, respectively. Amylolytic hydrolysis of rice starch granules for 120 min could produce porous rice starch (PRs), enabling the encapsulation of iNPs to form iNPs-loaded PRs (PRs@iNPs). The iNPs were much easier encapsulated by PRs than the free insulin at an equivalent insulin concentration of 0.60 mg/mL. When PRs@iNPs mixed with different concentrations of sodium alginate, the calcium chloride initiated the cross-linking of calcium alginate, and the formation of calcium alginate layer not only protected the starch from the amylase hydrolysis, but also maintained the sustained release of insulin in the simulated gastrointestinal fluid. Moreover, both starch digestion and insulin releasing can be controlled by the concentration of sodium alginate used. This study may provide an efficient benign technology to produce microcapsules loaded with insulin nanoparticles for controllable releasing properties.
研究领域:
药剂学
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Qing Liu,Fang Li,Na Ji,Lei Dai,Liu Xiong,Qingjie Sun
Natural amphiphilic polymer micelles are of great interest as a carrier of hydrophobic functional ingredients due to their relatively easy synthesis, biocompatibility, and extensive sources. This study modified debranched starch (DBS) with acetic anhydride to introduce the hydrophobic acetyl group, and micelles were then obtained through the self-assembly of acetylated debranched starch (ADBS). The degree of substitution (DS) and the structural and thermal properties of ADBS were systematically investigated; the maximum DS value was 0.17. Proton nuclear magnetic resonance spectra of ADBS confirmed the presence of acetyl methyl protons, and Fourier transform infrared spectra exhibited a new absorption band of carbonyl C = O vibration at 1730 cm −1 due to the introduction of acetyl. The developed micelles were mostly spherical, and the particle size was about 50–100 nm. The critical micelle concentration was 0.45 mg/mL. Curcumin was incorporated into the micelles successfully, and the curcumin-loaded micelles exhibited sustained release properties in simulated gastric and intestinal fluids.
研究领域:
食品科学与工程
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
COLLOID AND POLYMER SCIENCE
·
2021
·Vol. 299
·期 10
·1557-1566
·
IF 1.931
Hua Chenghuan,Yu Wenjie,Yang Mingyue,Cai Qun,Gao Tian,Zhang Silong,Xu Huan,He Huan,Peng Na,Liu Yi
New nanocomplexes containing curcumin-loaded casein micelles and surface-coated pectin via electrostatic interaction were fabricated. Curcumin-loaded nanocomplexes prepared at pH 4 showed the most excellent performance than that at pH 3 and pH 2, which had an average size of 266.4 nm (polydispersity index = 0.193) and loading capacity of 93%. The morphology of nanocomplexes exhibited as monodisperse spherical nanoparticles observed by SEM and TEM. The experiment of anti-oxidant activity demonstrated that nanocomplexes tremendously kept the bioactivity of curcumin. Moreover, in vitro release behaviors of curcumin from casein-pectin nanocomplexes indicated that the surface coating layer of pectin obviously delayed the release of curcumin in simulated gastric fluid, and the controlled release ability in simulated intestinal fluid was greatly enhanced. The casein-pectin nanocomplexes exhibited as a promising oral delivery vehicle of bioactive ingredients in food and pharmacy. Graphical abstract
研究领域:
食品科学
·
药学
·
生物医学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2018
·Vol. 25
·期 7
·1-12
·
IF 1.434
Hu Yan,Zhang Shangwen,Han Dandan,Ding Zongxian,Zeng Suying,Xiao Xincai
We prepared a hydroxypropyl methyl cellulose-sodium alginate (HPMC-SA) composite hydrogel with a membrane covering the semi-interpenetrating network based on a semi-synthetic polymer hydroxypropyl methyl cellulose (HPMC) and a natural polymer sodium alginate (SA) by Ca 2+ crosslinking and polyelectrolyte complexation with chitosan (CS) covering the hydrogel surface. The physiochemical properties of HPMC-SA hydrogels were evaluated by scanning electron microscopy, infrared spectrum, X-ray diffraction, and thermogravimetric analysis. The swelling ratio of the HPMC-SA composite hydrogel in simulated gastrointestinal fluid was measured. The drug release behavior of the HPMC-SA composite hydrogel for macro-molecular and small-molecule drugs was evaluated by using bovine serum albumin, metformin hydrochloride, and indomethacin as model drugs. The results showed that the HPMC-SA hydrogel had good water absorption and degradability, an increased swelling ratio of 55, and a prolonged time for maximum swelling degree of 50 h. Moreover, the hydrogel exhibited higher drug-loading capacity and improvements in the sustained release of bio-macromolecules, demonstrating its potential as a drug carrier for biomedical applications.
研究领域:
药剂学
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2019
·
IF 4.259
Ji Rui,Wu Jiahui,Zhang Junliang,Wang Tao,Zhang Xudong,Shao Lei,Chen Daijie,Wang Jian
Bifidobacteria are considered one of the most important intestinal probiotics because of their significant health impact. However, this ability is usually limited by gastrointestinal fluid and temperature sensitivity. Emulsification and internal gelation is an encapsulation technique with great potential for probiotic protection during storage and the gastrointestinal transit process. This study prepared microcapsules using an emulsification and internal gelation encapsulation method with sodium alginate, chitosan, and Bifidobacterium longum as wall material, coating material, and experimental strain, respectively. Optical, scanning electron, and focal microscopes were used to observe the microcapsule surface morphology and internal viable cell distribution, and a laser particle size analyzer and zeta potentiometer were used to evaluate the chitosan-coating characteristics. In addition, microcapsule probiotic viability after storage, heat treatment, and simulated gastrointestinal fluid treatment were examined. Alginate microcapsules and chitosan-coated alginate microcapsules both had balling properties and uniform bacterial distribution. The latter kept its balling properties after freeze-drying, verified by scanning electronic microscopy (SEM), and had a clear external coating, observed by an optical microscope. The particle size of chitosan-coated alginate microcapsules was slightly larger than the uncoated microcapsules. The zeta potential of alginate and chitosan-coated alginate microcapsules was negative and positive, respectively. Heat, acid and bile salt tolerance, and stability tests revealed that the decrease of viable cells in the chitosan-coated alginate microcapsule group was significantly lower than that in uncoated microcapsules. These experimental results indicate that the chitosan-coated alginate microcapsules protect B. longum from gastrointestinal fluid and high-temperature conditions.
研究领域:
食品科学
·
微生物学
·
生物工程
✓ 已验证
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2024
·
IF 3.8
Jingwen Xu,Guangwen Luo,Yue Zhang,Kaiqiang Lv,Sijuan Wang,Zuheng Shan,Xiang Gao,Guoliang Li
To improve the limited bioavailability of resveratrol (RSV) due to its poor water solubility, stability and bioaccessibility, there is an urgent need for the development of food-grade delivery systems. Herein, RSV was loaded into Spirulina platensis ( SP ) through passive diffusion, electrostatic and hydrogen bond interactions to create a Spirulina platensis ( SP )-based edible vehicle called SP @RSV, with a maximum loading content of 45.36 ± 1.08 % and loading efficiency of 86.72 ± 4.41 %. RSV was endowed with ultraviolet resistance after loading. SP @RSV showed both a broad-spectrum scavenging capacity against free radicals after digestion of gastrointestinal fluids, and a high bioaccessibility. Furthermore, SP @RSV could significantly prolong the life span of Caenorhabditis elegans by enhancing the activity of antioxidases and without causing acute toxicity. Besides, SP @RSV-contained jelly was prepared and sensory evaluated to showcase its potential utilization in the nutraceuticals industry. These findings confirm the feasibility of using SP as a vehicle to enhance the stability, intestinal targeting and bioaccessibility of RSV.
研究领域:
食品科学
·
药理学
·
生物技术
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Shanshan Tie,Wentao Su,Xuedi Zhang,Yannan Chen,Xue Zhao,Mingqian Tan
The purpose of this study was to construct a delivery system using a microfluidic chip to protect procyanidins (PCs) and to achieve their pH-controlled release in simulated gastrointestinal fluid. The microfluidic chip was designed and fabricated to generate water-in-water-in-oil (W/W/O) templates for the preparation of sodium alginate/chitosan microparticles with a uniform size and core–shell structure, using an internal–external gelation method. Compared with free PCs, the stability of PCs embedded in microparticles was improved and a pH stimulus-responsive release of PCs from microparticles was observed under neutral pH conditions. The delivery system of microparticles was nontoxic and showed an inhibitory effect on the decrease of mitochondrial membrane potential in Caco-2 cells caused by H2O2 and acrylamide. This work provided a method for fabricating compact microfluidic chips to prepare a pH stimulus-responsive PCs delivery system with improved stability, which may have potential applications in the delivery of other nutrients.
研究领域:
药物递送系统
·
微流控技术
·
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）