BIOCATALYSIS AND BIOTRANSFORMATION
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2020
·
IF 1.863
Chou Li,Qingzhi Ji,Tongyao He,Yingying Liu,Yunqing Ma
Deconjugation of bile salts in vivo not only promotes the cholesterol metabolism of the host, but also provides cellular carbon, nitrogen and sulphur for some probiotics. In this study, the gene of BSH from Bifidobacterium bifidum (ATCC 29521) was amplified and expressed using glutathione S-transferase (GST) Gene Fusion System. The enzyme was purified in a mild conditions without denaturation and renaturation, and the molecular mass was estimated to be 35 kDa. The optimum pH for the enzyme catalysis was 5.5. The BSH enzyme was stable from 37 to 57 °C. In addition, this enzyme was completely inhibited by Cu2+, while many other metal ions only gave moderate inhibition. Hydrolysis efficiency of BSH was quantified in two ways: ninhydrin colouration method and high-performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD). The hydrolysis process was clearly observed through real-time picture monitoring. The activity of BSH on glycine-conjugated bile salts was better than on taurine-conjugated bile salts. The proportion of hydrolysis for both sodium glycochenodeoxycholate (GCDCA) and sodium glycoursodeoxycholate (GUDCA) were more than 90% in 60 min. However, the proportion of hydrolysis for sodium taurochenodeoxycholate (TCDCA) and sodium tauroursodeoxycholate (TUDCA) were only 68% and 43% at the same condition. The study of this paper provides a better choice for hydrolysis of glycine-conjugated bile salts.HighlightsThe specificity of BSH was high for glycine-conjugated bile salts.Hydrolysis process of bile acid was obtained through real-time picture monitoring.Hydrolysis efficiency of BSH was quantified in two ways: ninhydrin colouration method and HPLC-ELSD.
研究领域:
微生物学
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酶学
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益生菌研究
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代谢
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2019
·Vol. 55
·期 2
·729-741
·
IF 2.281
Junmeng Lu,Piaohan Tu,Yanwen Feng,Na Li,Xiankang Xu,Kexuan Li,Yixin Yao,Jianzhong Han,Weilin Liu
Summary In this study, the influence of dietary compounds, including antioxidants (ethylenediaminetetraacid, ascorbic acid, catechin), chitosan and lactoferrin, on the stability of liposomes during in vitro adult and infant digestion was investigated in terms of formation of thiobarbituric acid-reactive substances (TBARS) and free fatty acid release. Liposomes were more sensitive to degradation in adults than in infants, suggesting lower pH (1.5) and higher concentration of pancreatic enzymes (3.2 mg mL −1 ) and bile salts (5 mg mL −1 ) that indicated greater damage to the structure of liposomes. Compared to ethylenediaminetetraacid and ascorbic acid, catechin presented the most apparent protective effect against liposomal oxidation by scavenging free radicals. Chitosan promoted the formation of TBARS, while lactoferrin facilitated the hydrolysis rate of liposomes under both adult and infant conditions. This study provided an insight into the development of healthcare products and functional foods related to liposomes for special populations.
研究领域:
食品科学
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营养学
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DOI
高影响因子
Journal of Environmental Sciences
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2014
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IF 1.922
Xuesong Kong,Shixiang Feng,Xu Zhang,Yan Li
The effects of bile salts (sodium cholate and sodium deoxycholate, 0–20 mmol/L), divalent cations (Ca 2+ , Mg 2+ , Cu 2+ and Zn 2+ , 0–20 mmol/L) or pH (3.0–10.0) on the adsorption of norfloxacin by three selected soils (Paddy_H, Paddy_G and Red_J) were systematically studied. Soil adsorption of norfloxacin follows a pseudo second-order kinetics model, and the maximum adsorption capacity has been determined from the nonlinear fit of the Langmuir isotherm model to be 88.8, 88.1 and 63.0 μmol/g for the adsorption onto Paddy_H, Paddy_G and Red_J, respectively. The results indicate that norfloxacin has a high adsorption affinity for the agricultural soils tested and that the organic content of these soils have at least a slight influence on this adsorption. The adsorption of norfloxacin to soils was strongly dependent on pH and exhibited a maximum at approximately pH 6. The presence of divalent cations prominently suppressed the adsorption of norfloxacin by paddy soils, which followed an order of Cu 2+ > Mg 2+ > Ca 2+ > Zn 2+ , and by red soil, which followed an order of Cu 2+ > Zn 2+ > Ca 2+ > Mg 2+ . The adsorption of norfloxacin (by the soils studied) sharply decreased as the amount of bile salts was increased. For uncharged norfloxacin at environmentally relevant pH values, such factors as soil type, exogenous divalent cations and macromolecules significantly altered the environmental fate and transport of norfloxacin between aquatic and soil interfaces.
研究领域:
环境科学
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土壤学
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DOI
PubMed
高影响因子
ACTA PHYSICO-CHIMICA SINICA
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2013
·Vol. 29
·期 11
·2415-2421
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IF 0.869
ZHU Li-Jie,HUANG Dan-Dan,LI Qin-Tang,XU Guan-Chen,CHEN Xiao
Nanofibers and vesicles are fabricated in aqueous solution by self-assembly of complexes of cationic Gemini surfactants ((C
s
H2s
-α,ω-(Me2N+C
m
H2m+1Br-)2,
m-s-m) and anionic bile salts (BS). Their morphology, structure, and properties are characterized by polarized optical microscopy (POM), transmission electron microscopy (TEM), field-emission scanning electron microscopy (FE-SEM), and X-ray powder diffraction (XRD). The morphology of
these aggregates was significantly influenced by minor structural changes of the building blocks, including the spacer and alkyl chain lengths of m-s-m, and the hydroxyl group number and position on the steroid skeleton of the BS. The formation of these aggregates is considered to arise
mainly fromelectrostatic interactions, with contributions fromhydrogen bonding and hydrophobic interactions. The results obtained may be helpful for understanding the mechanisms of ionic self-assembly and aid the design of novel supramolecular aggregates.
研究领域:
材料科学
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生物化学
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DOI
高影响因子
MOLECULES
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2019
·Vol. 24
·期 24
·4508
·
IF 3.06
Jing Wang,Xu Xu,Hao Chen,Shuai-Shuai Zhang,Yin-Xian Peng
Au nanoparticles (NPs) were prepared by UV light irradiation of a mixed solution of HAuCl4and sodium deoxycholate (NaDC) under alkaline condition, in which NaDC served as both reducing agent and capping agent. The reaction was monitored by circular dichroism (CD) spectra, and it was found that the formed gold NPs could catalyze the oxidation of NaDC. A CD signal at ~283 nm in the UV region was observed for the oxidation product of NaDC. The intensity of the CD signal of the oxidation product was enhanced gradually with the reaction time. Electrospray ionization (ESI) mass spectra and nuclear magnetic resonance (NMR) spectra were carried out to determine the chemical composition of the oxidation product, revealing that NaDC was selectively oxidized to sodium 3-keto-12-hydroxy-cholanate (3-KHC). The chiral discrimination abilities of the micelles of NaDC and its oxidation product, 3-KHC, were investigated by using chiral model moleculesR,S-1,1′-Binaphthyl-2,2′-diyl hydrogenphosphate (R,S-BNDHP). Compared with NaDC, the micelles of 3-KHC displayed higher binding ability to the chiral model molecules. In addition, the difference in binding affinity of 3-KHC micelles towardsR,S-isomer was observed, andS-isomer was shown to preferentially bind to the micelles.Keywords:noble metal nanoparticles;catalytical oxidation;chiral recognition;bile salts;supramolecular chemistry
研究领域:
生物学
·
化学
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
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2012
·
IF 4.269
Mengmeng Niu,Yi Lu,Lars Hovgaard,Peipei Guan,Yanan Tan,Ruyue Lian,Jianping Qi,Wei Wu
Oral delivery of protein or polypeptide drugs remains a challenge due to gastric and enzymatic degradation as well as poor permeation across the intestinal epithelia. In this study, liposomes containing bile salts were developed as a new oral insulin delivery system. The primary goal was to investigate the effect of cholate type, particle size and dosage of the liposomes on the hypoglycemic activity and oral bioavailability. Liposomes containing sodium glycocholate (SGC), sodium taurocholate (STC) or sodium deoxycholate (SDC) were prepared by a reversed-phase evaporation method. After oral administration, all liposomes elicited a certain degree of hypoglycemic effect in parallel with an increase in blood insulin level. The highest oral bioavailability of approximately 8.5% and 11.0% could be observed with subcutaneous insulin as reference for SGC-liposomes in non-diabetic and diabetic rats, respectively. Insulin-loaded liposomes showed slower and sustained action over a period of over 20 h with peak time around 8–12 h. SGC-liposomes showed higher oral bioavailability than liposomes containing STC or SDC and conventional liposomes. The hypoglycemic effect was size-dependent with the highest at 150 nm or 400 nm and was proportionally correlated to the administered dose. The results supported the hypothesis of insulin absorption as intact liposomes.
研究领域:
药剂学
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生物医学
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DOI
高影响因子
Journal of Functional Foods
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2016
·
IF 3.973
Chun-Feng Guo,Di Zhao,Ya-Hong Yuan,Tian-Li Yue,Bin Liu,Jing-Yan Li
The hypocholesterolaemic effects of milk fermented by Lactobacillus casei F0822 with bile salt hydrolase activity were investigated in hamsters fed a cholesterol-rich diet. Fermented milk (FM) exhibited dose-dependent hypocholesterolaemic activity. Sterilized and low-dose FM did not significantly affect the lipid profile of hamsters, whereas high-dose FM significantly reduced the levels of serum total cholesterol (TC) and non-high-density lipoprotein cholesterol (non-HDL-C), as well as hepatic TC and esterified cholesterol (EC). The daily faecal total bile acid excretion was significantly negatively correlated with the serum TC and non-HDL-C, as well as with the hepatic TC and EC. High-dose FM significantly enhanced daily faecal total bile acid excretion, which attributed to the hydrolysis of conjugated bile acids and the binding of free bile acids by the cells of strain F0822. These data suggest that the FM may serve as a functional food and has the potential to regulate cholesterol metabolism in humans.
研究领域:
营养学
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微生物学
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代谢组学
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产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
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2014
·
IF 4.28
Sha Cai,Meng Zhao,Yapeng Fang,Katsuyoshi Nishinari,Glyn O. Phillips,Fatang Jiang
Emulsification/internal gelation is an effective encapsulation method to protect probiotics from adverse environment. In this paper, microcapsules of Lactobacillus acidophilus CGMCC1.2686 were prepared by emulsification and gelation of alginate solutions with slow release of Ca 2+ ions from calcium carbonate (CaCO 3 ) and calcium disodium ethylenediaminetetraacetate (Ca-EDTA). Physical characterization of the two types of microcapsules showed that alginate-Ca-EDTA microcapsule was more uniform in size than alginate-CaCO 3 , with a span factor of 0.96 and 1.20, respectively. Mechanical measurements demonstrated that alginate-CaCO 3 microcapsule was more robust and elastic, which was supported by its denser structure as observed by electron scanning microscopy. Bacteria encapsulation yields in the two microcapsules were similar, 37.9% for alginate-CaCO 3 and 36.9% for alginate-Ca-EDTA. However, alginate-CaCO 3 microcapsule exhibited much higher cell survivals in both simulated gastric juice and bile salts solution (22.2% and 2.6*10 −2 %, respectively) than alginate-Ca-EDTA (7.1% and 0%, respectively), indicating a more effective protection of L. acidophilus CGMCC1.2686. The protection efficiency was discussed in relation to the mechanical properties of the microcapsules and the calcium sources used.
研究领域:
食品科学
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微生物学
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DOI
高影响因子
JOURNAL OF CEREAL SCIENCE
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2017
·
IF 2.223
Yu Zhu,Jiaxi Chu,Zhaoxin Lu,Fengxia Lv,Xiaomei Bie,Chong Zhang,Haizhen Zhao
Agricultural byproduct-foxtail millet ( Setaria italic ) bran was explored to extract dietary fiber by enzymatic methods and the physicochemical and functional properties of foxtail millet bran dietary fiber (FMBDF) were investigated. Results revealed that foxtail millet bran was a good source for dietary fiber production. The studies of physicochemical and functional properties showed that the water holding capacity and swelling power of FMBDF were 3.24 g/g and 2.06 ml/g, respectively. Meanwhile, FMBDF exhibited good adsorption capacities to lipophilic substances such as lard (3.34 g/g), peanut oil (2.32 g/g) and cholesterol (5.19 mg/g). Its bile salts adsorption capacity (143.03 μmol/g for sodium cholate and 76.65 μmol/g for sodium taurocholate) indirectly reflected its cholesterol-lowering effect. The chemical composition and microstructure studies of FMBDF demonstrated that these had a lot to do with its functional properties. The results indicate that FMBDF has a great potential to be used as functional ingredient in food products.
研究领域:
食品科学
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营养学
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产品关联
DOI
高影响因子
Chinese Journal of Natural Medicines
·
2016
·
IF 1.382
Zhuo-Wei SHEN,Meng-Yue LUO,Hai-Hong HU,Hui ZHOU,Hui-Di JIANG,Lu-Shan YU,Su ZENG
NTCP is specifically expressed on the basolateral membrane of hepatocytes, participating in the enterohepatic circulation of bile salts, especially conjugated bile salts, to maintain bile salts homeostasis. In addition, recent studies have found that NTCP is a functional receptor of HBV and HDV. Therefore, it is important to study the interaction between drugs and NTCP and identify the inhibitors/substrates of NTCP. In the present study, a LLC-PK1 cell model stably expressing human NTCP was established, which was simple and suitable for high throughput screening, and utilized to screen and verify the potential inhibitors of NTCP from 102 herbal medicinal ingredients. The results showed that ginkgolic acid (GA) (13 : 0), GA (15 : 1), GA (17 : 1), erythrosine B, silibinin, and emodin have inhibitory effects on NTCP uptake of TCNa in a concentration-dependent manner. Among them, GA (13 : 0) and GA (15 : 1) exhibited the stronger inhibitory effects, with IC 50 values being less than 8.3 and 13.5 μmol·L −1 , respectively, than the classical inhibitor, cyclosporin A (CsA) (IC 50 = 20.33 μmol·L −1 ). Further research demonstrated that GA (13 : 0), GA (15 : 1), GA (17 : 1), silibinin, and emodin were not substrates of NTCP. These findings might contribute to a better understanding of the disposition of the herbal ingredients in vivo , especially in biliary excretion.
研究领域:
药理学
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肝脏病学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Jiahui Chen,Minshan Guo,Runhui Fan,Yayun Peng,Ting Cai
Cocrystal engineering has become increasingly prevalent as an efficient and versatile route to overcome solubility limitations and improve the oral bioavailability of poorly soluble drugs. However, the unwanted solution-mediated phase transformation of cocrystals to less soluble parent drugs in the gastrointestinal tract may fail to deliver the drug in the body in a sufficient quantity. This study explores how bile salt sodium taurocholate (STC) affects the dissolution performance of indomethacin-saccharin (IMC-SAC) cocrystals. Fluorescence microscopy was used for in situ monitoring of the phase transformation of IMC-SAC cocrystals in the presence and absence of STC during dissolution. Intrinsic dissolution tests, atomic force microscopy and energy dispersive X-ray spectroscopy revealed that there was a significant relationship between the coformer release rate and the kinetics of the phase transformation of cocrystals. The addition of low-concentration STC in the buffer significantly inhibited the phase transformation of IMC-SAC cocrystals by retarding the release of SAC from cocrystals, leading to enhanced release rates of IMC. A molecular dynamics simulation revealed that preferential adsorption of STC molecules on the cocrystal surface may be responsible for the suppression of drastic release of SAC from the cocrystal lattice, thus preventing a rapid crystallization of IMC at the solid–liquid interface. The presence of STC in the buffer could also prolong the supersaturation of IMC, accounting for the overall improved in vitro and in vivo performance of IMC-SAC cocrystals. This study provides a deeper insight into the mechanisms of solution-mediated phase transformation of cocrystals in the presence of endogenous surfactant, which could also be used as an effective strategy to improve the absorption performance of pharmaceutical cocrystals by minimizing the risk of phase transformation during dissolution.
研究领域:
药剂学
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生物化学
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产品关联
DOI
高影响因子
Food Science and Human Wellness
·
2024
·
IF 5.6
Hyemin Oh,Yohan Yoon
This study investigated if the variation in the effect of anti-cholesterol (AC) treatment on individual mice are related to gut microbiome composition. The bile salt hydrolase (BSH) activity of 23 commercial fermented milk products was examined to select a fermented milk product for AC treatment. Mice were fed to different diets for 6 weeks: high-fat (660 % of total calories from fat; D1), high-dietary fibre (20 % cellulose; D2), and low-fat (17.2 % of total calories from fat; D3) diets to change their gut microbiomes. Subsequently, faecal microbiome was transplanted (FMT) into mice treated with high cholesterol diet contained 2 % cholesterol, followed by AC or non-AC (sterile tap water, STW) treatments. Control groups with normal (NC) and high-cholesterol diets (PC) were prepared for both AC and STW treatment. All experimental groups were subjected to serum and liver cholesterol, cholesterol metabolism-related (CMR) gene expression, and intestinal microbiome analyses. D3-FMT mice showed the most significant enhancements in cholesterol ratio and decreased hepatic cholesterol levels with AC treatment. Moreover, upregulation of the Cyp7a1 gene expression was observed in this group. Furthermore, the intestinal microbiome analysis indicated higher abundances of BSH-producing Eubacterium , Bifidobacterium , and Parabacteroides in the D3-FMT + AC group compare to others, potentially contributing to increased bile acid synthesis.
研究领域:
医学
·
微生物学
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产品关联
DOI
高影响因子
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