BIOLOGICAL CONTROL
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2022
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IF 3.857
Meilin Wei,Solairaj Dhanasekaran,Guillaume Legrand Ngolong Ngea,Esa Abiso Godana,Xiaoyun Zhang,Qiya Yang,Xiangfeng Zheng,Hongyin Zhang
Ochratoxin A (OTA), a widespread mycotoxin contaminant in food and feed, threatens human and animal safety and causes significant economic losses. The aims of the present study were to investigate the elimination properties of Cryptococcus podzolicus Y3 on OTA and its co-degradation efficiency on citrinin (CIT) and OTA. C . podzolicus Y3 eliminated 100% OTA (1 μg/mL) in the PM media within 5 d and in grape juice media within 3 d by intracellular substances. The results of HPLC-FLD and LC-MS showed that OTA was efficiently eliminated into non-toxic ochratoxin α (OTα). The substances responsible for OTA degradation were thermostable and worked effectively at 4 °C and 28 °C, and intracellular proteins were participated in the elimination of OTA. In addition, the results showed that C. podzolicus Y3 degraded OTA and CIT together in NYDB media, although the degradation efficiency was lower than single mycotoxin degradation. Generally, our study highlighted that C. podzolicus Y3 could be a potential strain to eliminate OTA and CIT to secure food products and enhance food safety.
研究领域:
食品科学
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毒理学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD MICROBIOLOGY
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2021
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IF 5.516
Shilin Wu,Xueming Xu,Na Yang,Yamei Jin,Zhengyu Jin,Zhengjun Xie
Non-conventional heating technology based on electric fields can be utilized to process liquid foods . In this study, the induced electric field (IEF) was investigated to clarify its inactivation mechanism on E.coli . Staining results show that inactivation of E.coli by IEF can be attributed to the reversible destruction of the cell membrane, followed by the denaturation of intracellular enzymes, and finally the irreversible rupture of the cell membrane. The increased levels of extracellular proteins and nucleic acids were also observed. IEF treatment at 400 Hz and 800 V (or 53 V/cm) results in a reduction of 4.5 log CFU·mL −1 in the number of E.coli . Storage life analysis shows that IEF treatment can improve the stability of apple juice and the content of bioactive components. Thus, IEF is a potential technique for liquid food processing.
研究领域:
食品科学
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微生物学
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非传统加热技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2018
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IF 8.097
Lin Hou,Yinling Zhang,Xuemei Yang,Chunyu Tian,Yingshan Yan,Hongling Zhang,Jinjin Shi,Huijuan Zhang,Zhenzhong Zhang
Nitric oxide (NO) is an important biological messenger implicated in tumor therapy. However, current NO release systems suffer from some disadvantages, such as hydrolysis during blood circulation, poor specificity, and robust irradiation for stimuli. Accordingly, we constructed an intracellular enzyme-triggered NO-generator to achieve tumor cytoplasm-specific disruption and localized rapid drug release. Diethylamine NONOate (DEA/NO) was used as a NO donor and conjugated with hyaluronic acid (HA) to form self-assembly micelle (HA-DNB-DEA/NO), and encapsulate chemotherapeutic agent (doxorubicin (DOX)) into its hydrophobic core (DOX@HA-DNB-DEA/NO). After HA receptor mediated internalization into tumor cells, HA shell would undergo digestion into small conjugated pieces by hyaluronidase. Meanwhile, DOX@HA-DNB-DEA/NO also responded to the intratumoral overexpressed glutathion and glutathione S-transferase π, leading to the intracellular NO production and controlled DOX rapid release. In vitro and in vivo results proved the enzyme-dependent and enhanced targeting delivery profile, and demonstrated that NO and DOX could colocate in specific tumor site, which provided a precondition for exerting their synergistic efficacy. Moreover, expression of p53 protein was upregulated in tumor tissue after treatment, indicating that NO induced cell apoptosis mediated by tumor suppressor gene p53. Overall, this intelligent drug loaded NO-generator might perform as an enhancer to realize better clinical outcomes.
研究领域:
药理学
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癌症研究
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药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
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2019
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IF 5.714
Li Lu,Qiwei Chai,Shanying He,Chunping Yang,Dong Zhang
Plant-endophyte synergism has been demonstrated to play a key role in the phytoremediation of contaminated water and soil. Phytoalexins, a type of chemical component in the plant apoplast, can be produced by plants in response to stimulation by endophytes. Phytoalexins may have distinct effects on the nutritional and metabolic functions of endophytes; however, direct evidence is not available to prove the effect of phytoalexins on the hydrophobic organic contaminants (HOC)-degradation activity of endophytes. In this paper, three different types of phytoalexins, coumarin, resveratrol and rutin, were selected to study their effect on the removal of polycyclic aromatic hydrocarbons (PAHs) by an endophytic bacterium Methylobacterium extorquens C1. The effects of the three phytoalexins on bacterial sorption and intracellular enzymatic activities were tested to further analyze the mechanism by which the phytoalexins affect the PAH degradation performance of M. extorquens C1. The results showed that the removal rate of PAHs by M. extorquens C1 increased in the presence of low levels of the three phytoalexins. The most effective concentrations of coumarin, resveratrol and rutin were 0.20, 0.15, and 0.25 mg/L, respectively, and the removal rate of PAHs was increased by approximately 18.3–35.0%. At the optimal concentrations, the three phytoalexins significantly promoted the sorption of PAHs by M. extorquens C1, and also enhanced the activities of catechol dioxygenases and dehydrogenase of M. extorquens C1. The positive effect of phytoalexins on both bacterial sorption and intracellular enzymatic activities promotes the overall removal of PAHs from endophytes. These results may deepen our understanding of plant-microbe cooperative mechanisms in the degradation of organic pollutants and provide a new approach for chemically enhanced bioremediation in the future.
研究领域:
环境科学
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微生物学
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植物科学
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生物修复
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）