Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Shengmei Song,Junhui Hu,Minglu Li,Xiaojuan Gong,Chuan Dong,Shaomin Shuang
The fluorescent boron, nitrogen and sulfur co-doped carbon dots (BNSCDs) were prepared by simple hydrothermal reaction of 4-carboxyphenylboronic acid and 2,5-diaminobenzenesulfonic acid at 200 °C for 8 h. The fluorescence of the BNSCDs could be quenched by Fe 3+ based on the electron transfer between Fe 3+ and BNSCDs, so a label-free, good selectivity and high sensitivity method for Fe 3+ determination was established with linear range and LOD of 1.5–692 μmol/L and 87 nmol/L, respectively. And then the fluorescent probe was employed for detection of Fe 3+ in tap water, coal gangue, fly ash and food samples successfully. Moreover, the as-prepared BNSCDs could serve as a novel pH fluorescent probe in the range of pH 1.60–7.00, which could be attributed to the proton transfer of carboxyl groups on the surface of BNSCDs. More importantly, the pH fluorescent probe possesses fast, real-time and low toxicity, applying for intracellular pH fluorescence imaging in HIC, HIEC, LO2 and SMMC7721 cells. In view of its simplicity, timely response and outstanding compatibility, the as-fabricated BNSCDs show the potential applications in water quality and solid waste monitoring, food detection, real-time measuring of intracellular pH change in vitro.
研究领域:
分析化学
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生物医学工程
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环境科学
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DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 56
·33635-33641
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IF 3.119
Meihua Lin,Xin Ma,Shijun Lin,Xiaojin Zhang,Yu Dai,Fan Xia
Carbon dots (CDs) as fluorescent probes have been widely exploited to detect biomarkers, however, tedious surface modification of CDs is generally required to achieve a relatively good detection ability. Here, we synthesized N-doped carbon dots (N-CDs) from triethylenetetramine (TETA) and m-phenylenediamine (m-PD) using a one-step hydrothermal method. When the pH increases from 3 to 11, the fluorescence intensity of the N-CDs gradually decreases. Furthermore, it displays a linear response to the physiological pH range of 5–8. Au3+ is reduced by amino groups on the surface of N-CDs to generate gold nanoparticles (AuNPs), causing fluorescence quenching of the N-CDs. If glutathione (GSH) is then added, the fluorescence of the N-CDs is recovered. The fluorescence intensity of the N-CDs is linearly correlated with the GSH concentration in the range of 50–400 μM with a limit of detection (LOD) of 7.83 μM. The fluorescence probe was used to distinguish cancer cells from normal cells using pH and to evaluate intracellular GSH. This work expands the application of CDs in multicomponent detection and provides a facile fluorescent probe for the detection of intracellular pH and GSH.
研究领域:
生物化学
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分析化学
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医学检测
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Lingling Li,Yang Li,Yijing Dang,Tingting Chen,Ao Zhang,Chunyong Ding,Zhiai Xu
Intracellular pH is a key physiological factor for controlling the activities and functions of cells and lysosome is a vital subcellular organelle. Thus, developing a novel lysosome-targeting fluorescence probe for selective and sensitive detection of lysosomal pH in living cells is very important. In this work, we synthesized a series of fluorescence probes based on imidazole-fused benzothiadiazole . The optical properties of these probes were easily adjusted by modifying the substituents with different electron-withdrawing/donating ability in imidazole moiety. All of them showed acid-response and decreased fluorescence intensity during pH values changing from 4.0 to 8.5. The introduction of morpholine group allowed them to specifically respond to the changes of lysosomal pH. Among them, probe MIBTAA possessed a suitable p K a value (5.3) and showed good linear response to pH (R 2 = 0.9918) with red emission when pH changed from 4.4 to 5.6. The probe was successfully applied for monitoring pH variation in living cells induced by proton-pump inhibitor Baf-A1 and chloroquine , indicating its great potential for pH imaging in biological applications.
研究领域:
生物化学
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细胞生物学
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医学影像学
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2021
·
IF 8.198
Xiaoyu Wang,Yishui Wang,Wei Pan,Jinping Wang,Xiaobo Sun
It is difficult to quantitatively explain the effects of intracellular conditions on bioreactions in cells because direct measurements in cells are not easily achieved. In this work, a relationship between the biosynthesis of intracellular polysaccharide and intracellular pH inside fungal cells was established by quantitatively determining them using proper methods, respectively. A fluorescent ratiometric pH probe was designed and synthesized for the detection of intracellular pH by conjugating a pH-sensitive moiety and pH-insensitive carbon dots (CDs) into one system. The intracellular pH values and intracellular polysaccharide contents inside the mycelia of Pholiota adipose fungus incubated under different conditions were individually analyzed by the ratiometric pH probe and a phenol-sulfuric acid method. Thereafter, a relationship was established using the content of polysaccharide in mycelia as ordinates and intracellular pH values as abscissa. As can be seen from the relationship, intracellular pH in the range 7.10–7.25 inside Pholiota adipose mycelia is the most suitable condition for the biosynthesis of intracellular polysaccharide.
研究领域:
生物化学
·
微生物学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Shengrui Zhang,Xiaohui Ji,Jin Liu,Qin Wang,Lingxia Jin
A new nanoprobe based on yellow-emissive carbon dots (Y-CDs) was developed for sensing full-range intracellular pH values. By using o -phenylenediamine as the raw material, Y-CDs with a quantum yield of 31% were prepared through a one-pot solvothermal carbonization method. The Y-CDs exhibited a distinctive fluorescence emission peak at 570 nm with excitation at 450 nm, showing a very large Stokes shift (120 nm). Notably, the nanoprobe revealed a linear relationship between fluorescence intensity and pH value within the range of pH 4.0 to 8.2, exhibiting the ability of this probe to monitor full-range intracellular pH variations. In addition, the nanosensor possessed excellent photostability and fluorescence reversibility in pH measurements and showed excellent selective detection of the influences of other biological species. The CD-based nanoprobe was successfully used to perform quantitative fluorescence imaging of intracellular pH variation, demonstrating its promise for application in cellular systems.
研究领域:
生物医学工程
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材料科学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Yutao Jia,Yanting Shen,Yanyan Zhu,Jing Wang
Lysosomes are the acidic organelles in the cells that play an important role in intracellular degradation and other various cellular functions. The pH disturbance of lysosomes will result in the lysosomal dysfunction and many lysosomal related diseases. In this work, we reported a methoxy-based covalent organic framework (TAPB-DMTP-COF) that a novel pH-responsive fluorescent probe for lysosomal pH imaging in cells. The prepared TAPB-DMTP-COF presented regular crystal structure, low toxicity and good pH responsive property. The rich imine structure in the material enabled pH-responsive properties of the TAPB-DMTP-COF and made it exhibited pH-dependent fluorescence response. Good detection linearity for pH measurements in aqueous solution was achieved by this probe. Moreover, the TAPB-DMTP-COF can be used for the selective lysosomal pH imaging. Confocal fluorescence imaging results demonstrated that the pH fluctuations (from 4.0 to 7.4) and the pH changes in lysosomes can be effectively monitored in situ by the developed probe. This study may provide a new avenue for the intracellular pH sensing, deep study and understanding about the mechanism of diseases related to abnormal lysosomal pH.
研究领域:
生物化学
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材料科学
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医学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2014
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IF 6.451
Changqin Ding,Yang Tian
The dysregulated pH is working as a mark of cancer. It is a challenge for developing a biosensor for targeted imaging in cancer cells and monitoring of intracellular pH . Here, a ratiometric fluorescence biosensor for pH determination was developed with targeted imaging into folate acceptor (FR)-rich cancer cells at the same time. AuNCs protected by bovine serum albumin (BSA) worked as reference fluorophore and fluorescein-isothiocyanate (FITC) acted as the response signal for pH. For targeted imaging of cancer cells, the AuNCs were simultaneously conjugated with folic acid (FA). The developed ratiometric biosensor can monitor pH with a wide linear range from 6.0–7.8 with a pKa at 6.84. Under every different pH condition , the probe showed high selectivity over various metal ions and amino acids with its fluorescence ratio stayed almost constant (<5%). It also showed good cyclic accuracy when pH switched between 6.0 and 8.0, as well as low cytotoxicity. The AuNC-based inorganic–organic nanohybrid biosensor showed good cell-permeability, low cytotoxicity, and long-term photostability. Accordingly, the pH biosensor was employed to gain targeted imaging in FR +ve Hela cells with FR −ve lung carcinoma cells A549 as comparison, and achieved to monitor the pH changes in Hela cells.
研究领域:
生物化学
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医学诊断
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纳米生物学
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DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2024
·Vol. 40
·期 2
·287-292
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IF 3.1
Li Yaohua,Wang Wei
Intracellular pH (pH i ) is a fundamental indicator of cellular physiological state, regulating cellular state and function, and has important research values. Although various probes for measuring intracellular pH were available, it is challenging to reflect pH i in real-time and reversible manners. Herein, we developed a whole-cell bioluminescent (BL) probe based on wild type BL bacteria, photobacterium phosphoreum ( P. phosphoreum ), to determine and image pH i . The dependence of BL intensity of P. phosphoreum on pH values of culture solutions was established. It was found that BL intensity could respond to the change of pH values rapidly and reversibly. We further revealed that P. phosphoreum maintained pH homeostasis in the extracellular pH (pH e ) within the range of 5.0–7.0, while intracellular pH homeostasis was destroyed at the alkaline pH e . This method opens up the enormous potential of BL bacteria as an alternative to fluorescence for monitoring and imaging pH i .
研究领域:
生物化学
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细胞生物学
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生物物理学
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微生物学
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生物医学工程
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DOI
高影响因子
LANGMUIR
·
2019
·
IF 3.683
Sihui He,Jiantao Yu,Fangfang Wang,Leilei Tian
Intracellular pH is a vital parameter which can reflect the physiological process, and the detection of intracellular pH with a high signal-to-noise ratio (SNR) remains a challenge. Compared to pH biosensors based on a single-wavelength signal, it is much easier to obtain better sensitivity and higher SNR from the biosensors by two-wavelength ratiometric signals. In this study, we used DNA-grafted graphene oxide (GO) to ratiometrically detect intracellular pH ranging from basic to acidic. A high SNR with a 35-fold difference in the ratiometric output has been achieved through careful optimization: (1) A high DNA conjugation yield of 45% has been gained through utilizing the partial double-stranded assembly strategy. (2) Herring sperm DNA (HSD) plays an important role in improving the sensitivity of the nanosystem by purifying and passivating the surface of GO; therefore, the concentration of HSD has been optimized to pursue the most sensitive ratiometric response. Apart from the ultrahigh SNR, fabricated GO-AR-Cy5/IFO-Cy3 exhibited excellent stability and biocompatibility in biological environments. Further experiments demonstrated that the nanosystem worked well in live cells in response to pH changes. It is possible to distinguish small pH differences and realize quantitative detection based on ratiometric fluorescence imaging by laser scanning confocal microscope analysis, which makes the nanosystem a promising candidate for further biological study and clinical applications.
研究领域:
生物技术
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纳米医学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2018
·Vol. 16
·期 48
·9402-9408
·
IF 3.423
Pan Li,Zhe Chen,Yishun Huang,Jing Li,Fan Xiao,Shiyao Zhai,Zhiming Wang,Xuanjun Zhang,Leilei Tian
A new DNA-based fluorescent probe, which is a hybrid molecule of an i-motif forming sequence (IFS) and mono-functionalized tetraphenylethene (TPE), has been synthesized and investigated. A distinct pH-responsive aggregation-induced emission (AIE) effect has been observed from this hybrid molecule, i.e. the fluorescence of TPE will be turned on when the IFS part folds up under acidic conditions. According to the fact that a hybrid molecule with a relatively rigid structure shows a more obvious AIE effect, and whose nano-sized aggregates are formed at a high concentration, we assume that the solubility of the hybrid molecule in water will be reduced due to IFS folding, resulting in aggregation and the resultant AIE effect. Finally, due to its excellent pH responsiveness, this DNA-based probe employing an AIEgen has been applied in monitoring intracellular pH.
研究领域:
生物化学
·
分子生物学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2022
·
IF 1.193
Xiao-Ping CHEN,Ya-Feng ZHUANG,Jian-Wei LIN,Jing-Hua CHEN,Zhi-Zhong HAN
Using citric acid and thiourea as precursors, sulfur-nitrogen co-doped dual emission carbon dots (DECDs) were prepared by a simple solvothermal method. At the excitation wavelength of 360 nm, DECDs have two emission peaks located at 465 nm (blue) and 592 nm (yellow-green) which are attributed by core and surface of DECDs. It is found that Fe 3+ ions can coordinate with oxygen-containing groups on DECDs, and then the electrons transfer from DECDs to Fe 3+ ions, which quench the fluorescence of DECDs. The fluorescence intensity is linear with the concentration of Fe 3+ ions in the range of 0–100 μM and the detection limit is 0.77 μM. Moreover, the designed sensor can be applied to detect Fe 3+ ions in tap water with high selectivity, which provides a basis for the quantitative detection of Fe 3+ ions. At the same time, DECDs can be used as a pH fluorescence probe. In the pH 2.5–11.5 range, the fluorescence increases with pH whilst the solution color changes, providing a visualization platform for pH detection. Moreover, the pH fluorescence probe can be used for intracellular pH fluorescence imaging in living cells, and DECDs have an excellent biocompatibility (e.g., cell viability remains about 80% after treatment for 24 h at 200 μg·mL −1 of DECDs). Therefore, DECDs has a certain application prospect in food detection, biological detection and determination of intracellular pH change.
研究领域:
化学
·
生物医学工程
·
分析化学
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Fengfeng Xue,Huifeng Zhao,Hui Liu,Jingjing Lou,Kailin Li,Zikang Wang,Lu An,Qiwei Tian
Iron-based chemodynamic therapy (CDT) exhibits commendable biocompatibility and selectivity, but its efficacy is constrained by the intracellular pH of tumors. To overcome this obstacle, we constructed a silica delivery platform loaded with autophagy-inducing reagents (rapamycin, RAPA) and iron-based Fenton reagents (Fe 3 O 4 ). This platform was utilized to explore a novel strategy that leverages autophagy to decrease tumor acidity, consequently boosting the effectiveness of CDT. Both in vitro and in vivo experiments revealed that RAPA prompted the generation of acidic organelles (e.g., autophagic vacuoles and autophagosomes), effectively changing the intracellular pH in the tumor microenvironment. Furthermore, RAPA-induced tumor acidification significantly amplified the efficacy of Fe 3 O 4 -based Fenton reactions, consequently increasing the effectiveness of Fe 3 O 4 -based CDT. This innovative approach, which leverages the interplay between autophagy induction and iron-based CDT, shows promise in overcoming the limitations posed by tumor pH, thus offering a more efficient approach to tumor treatments.
研究领域:
肿瘤学
·
生物化学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）