INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Dongdong Xu,Zongli Li,Zhennan Deng,Xin Nie,Yihuai Pan,Gu Cheng
Degradation profiles are critical for the optimal application of electrospun polymer nanofibers in tissue regeneration, wound healing, and drug delivery systems. In this study, natural and synthetic polymers and their composites were subjected to in vivo transplantation and in vitro treatment with lipases, macrophages, and acetic acid to evaluate their degradation patterns. The effects of environmental stimulation, surface wettability, and polymer components on the degradation profiles of the electrospinning poly(ε-caprolactone)/silk fibroin (PCL/SF) nanofibers were first evaluated. In vivo degradation study demonstrated that bulk degradation, characterized by the transition from microfibers to nanofibers, and surface erosion, characterized by fusion between the microfibers or direct erosion from both ends of the microfibers, occurred in the electrospun membranes; however, bulk degradation dominated their overall degradation. Furthermore, the degradation rates of the electrospun PCL/SF membranes varied according to the composition, morphology, and surface wettability of the composite membranes. After the incorporation of silk fibroin (SF), the degradation rate of the SF/PCL composite membranes was faster, accompanied by larger values of weight loss and molecular weight ( Mw ) loss when compared with that of the pure poly(ε-caprolactone) (PCL) membrane, indicating a close relationship between degradation rate and hydrophilicity of the electrospinning membranes. The in vitro experimental results demonstrated that enzymes and oxidation partially resulted in the surface erosion of the PCL/SF microfibers. Consequently, bulk degradation and surface erosion coordinated with each other to enhance the hydrophilicity of the electrospinning membranes and accelerate the in vivo degradation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2026
·
IF 6.6
Jihang Zhang,Yihui Luo,Siwen Ji,Jinming Zhang,Xiang Chen,Lei Ge,Jinglan Wu,Zhuotao Tan,Sheng Xu,Bin Wu,Hanjie Ying,Wei Zhuang
Sequential coupling of hydrophobic orientation and rapid covalent locking. • Immobilized TLL exhibits >4000% relative activity via conformational tuning. • Interfacial microenvironment regulates partitioning and methanol tolerance. • Biocatalyst demonstrates exceptional durability and efficient conversion. Efficient multiphase bioprocessing for sustainable lipid valorization and biodiesel synthesis relies on robust solid–liquid biointerfaces. Their rational design is fundamentally constrained by severe reaction–diffusion constraints and the intrinsic activity–stability trade-off. Herein, we report a synergistic “orient-and-lock” strategy on bifunctional mesoporous SBA-15 to decouple these limitations. Hydrophobic domains first drive the oriented adsorption and “lid-opening” hyperactivation of Thermomyces lanuginosus lipase, followed by rapid isocyanide-mediated covalent anchoring to kinetically trap this metastable state without compromising structural flexibility. Molecular dynamics simulations elucidate a critical microenvironmental compartmentalization effect. This spatial partitioning selectively enriches lipophilic substrates while isolating polar inhibitors through localized hydrogen-bond networks and hydrophobic compartments, thereby effectively reducing substrate transfer resistance and minimizing active site blockage. Consequently, the immobilized biocatalyst exhibits an unprecedented 40-fold enhancement in apparent specific activity and a 57-fold increase in catalytic efficiency ( k cat / K m ) compared to the free enzyme. Its excellent tolerance to methanol enables a highly efficient one-step methanol addition reaction. The system demonstrates exceptional operational robustness, retaining 80% activity over 25 consecutive cycles and achieving a 96.8% biodiesel yield, establishing a synergistic paradigm of conformational engineering and transport regulation for high-performance heterogeneous biocatalysts. Download: Download high-res image (141KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Bei Zhang,Xiaoying Zhang,Qiubian Yang,Peihang Chen,Jiaxin Xu,Jing Li,Fayong Gong,Jianyin Miao
Five novel lipid-lowering peptides were identified from Tartary buckwheat hydrolysate. • Molecular docking revealed peptide binding modes to 1LPB and 1F6W. • Peptides reduced lipid accumulation and improved lipid profiles in 3T3-L1 cells. • Peptides improved multiple lipid metabolism indicators in high-fat C. elegans . Highly active lipid-lowering peptides were isolated from Tartary buckwheat through enzymatic hydrolysis, ultrafiltration, and reversed-phase high-performance liquid chromatography (RP-HPLC). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and molecular docking techniques were employed to identify and screen lipid-lowering peptide monomers. The lipid-lowering effects and underlying mechanisms were evaluated through molecular docking visualization, in vitro chemical assays, the 3 T3-L1 cell model, and the Caenorhabditis elegans ( C. elegans ) model. Five novel peptides were identified: FHWDYPQA, FHWDYPQALE, LFHWDYPQA, LPSYSNAPYI, and NAIIGPRW. These peptides bind to receptor proteins 1LPB and 1F6W via hydrogen bonds, hydrophobic interactions, and electrostatic interactions. These peptides significantly inhibited pancreatic lipase activity, effectively reduced lipid accumulation in 3 T3-L1 cells, and lowered triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels. In addition, LI-10, FE-10, and LA-9 significantly elevated high-density lipoprotein cholesterol (HDL C) levels. Additionally, five peptides effectively reduced lipid accumulation in high-fat-fed C. elegans , decreased TG, glucose (GLU), and malondialdehyde (MDA) levels. Overall, these findings elucidate the lipid-lowering mechanisms of five novel peptides identified from Tartary buckwheat protein hydrolysates, providing a scientific foundation for the future development of Tartary buckwheat protein-derived functional products targeting hyperlipidemia. Download: Download high-res image (124KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Mohan Li,Chenhao Jiang,Gang Yang,Kewei Chen,Muying Du,Zsolt Zalán,Sameh Awad,Jianquan Kan
First evidence of restricted-release, matrix-bound AFB1 in PPC was proved. • Protein binding is the primary reason for AFB1 hide in PPC. • Matrix hydrolysis or force dissociation effectively releases bound AFB1. • Vitro digestion pretreatment hydrolyzed the matrix, improving detection accuracy. • Highlights critical exposure underestimation for AFB1 in protein-rich samples. Pressed peanut cake (PPC) is a valuable plant protein resource but is highly susceptible to aflatoxin B1 (AFB1) contamination. Its complex matrix can complicate the accurate analysis of AFB1. Digesting the samples in vitro prior to quantification resulted in a significant improvement in AFB1 detection value (25.81–39.40%) compared with untreated samples. Ultrafiltration analysis confirmed that conventional extraction solvents are susceptible to severe interference from the matrix. Molecular docking demonstrated that AFB1 can form complexes with the matrix. Protein hydrolysis leads to significantly higher detection values than starch or cellulose hydrolysis, a phenomenon attributed to the dominant role of protein in binding the toxin. Peanut protein entraps and locks AFB1 through synergistic hydrophobic and hydrogen bonding interactions, forming restricted matrix-bound AFB1. The study elucidated on the formation/ dissociation mechanism of matrix-bound AFB1 in PPC at the molecular level, emphasizing the underestimation of the actual exposure level of AFB1 in PPC. Download: Download high-res image (254KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2026
·Vol. 15
·期 4
·
IF 6
Kai Tong,Yuxue Liao,Yongqing Tang,Yaxin Luo,Xuan Liu,Dan Yu,Jingxuan Zhou,Chenjin Hou,Zhaoling Li
To address the limited functional diversity of traditional kombucha, this study utilized red yeast rice (RYR) as an alternative substrate and prepared three samples: black tea kombucha (KBT), black tea-red yeast rice mixed kombucha (KBL, at a 1:1 ratio), and red yeast rice kombucha (KRY). After 9 days of fermentation, KRY exhibited the lowest pH, the highest total acidity, and notable sugar metabolic activity. It exhibited in vitro inhibition rates of 82.8%, 78.2%, 70.3%, and 76.9% against cholesterol esterase, pancreatic lipase, α-glucosidase, and α-amylase, respectively, indicating potential hypoglycemic and hypolipidemic activities. In contrast, KBT maintained the strongest antioxidant capacity, with scavenging rates exceeding 90% against both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS). A total of 72 volatile flavor compounds (VFCs) were identified, with 7 key compounds enriched in KRY, which enhanced its sensory acceptance and received the highest scores in color, clarity, and aroma. Microbial community analysis revealed the post-fermentation dominance ofKomagataeibacter, Acetobacter, and Saccharomyces, which correlated positively with key VFCs. These findings indicate that RYR as a substrate enhances functional microbial growth, sugar metabolism, organic acid production, flavor enrichment, and in vitro inhibitory activity of enzymes associated with hypoglycemic and hypolipidemic effects.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Probiotics and Antimicrobial Proteins
·
2026
·
IF 5.3
Zhou Fangyan,Liang Liang,Xia Junfang,Wang Juan,Ma Wenrui,Liu Peizhao,Zhu Shiyu,Idris Munaweer,Huang Zihao,Zhu Jinfang,Wu Yun
With obesity posing a growing global health burden, probiotic interventions are gaining attention as potential therapeutic strategies. This study moves beyond conventional single-strain approaches by developing a rationally designed multi-strain consortium sourced from the under-explored microbial niche of traditional koumiss. Sixteen lactic acid bacterial strains were isolated and screened using key in vitro lipid-lowering indicators. Two high‑performing strains, Lactobacillus plantarum SR6 and Enterococcus faecalis SM5, were selected and formulated into a 1:1 composite (SR6‑SM5). The composite not only exhibited synergistically enhanced functional properties—including strong gastrointestinal tolerance, with a survival rate of over 95.7% in gastric juice and over 76% in intestinal juice, high adhesion potential (hydrophobicity 93.00 ± 0.25%; auto-aggregation 90.58 ± 0.31%), notable antioxidant activity (ABTS + and DPPH· scavenging rates > 87%), and antibacterial effects—but also demonstrated a comprehensive safety profile with no hemolytic, cytotoxic, or acute oral toxicity observed in mice. By integrating efficacy, safety, and probiotic suitability into a single tailored consortium, this work provides a novel, functionally enhanced probiotic candidate with strong potential for further development in obesity‑related functional foods.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2026
·
IF 5.6
Yangyang Song,Jie Song,Shuxian Liu,Jiyang Zhang,Ziyuan Liu,Haoyun Chen,Shiwen Wang,Tao Zhuang,Guisen Zhang
Depression and anxiety disorders are highly comorbid, yet their complex pathogenesis often limits the efficacy of monotherapy. Growing evidence implicates neuroinflammation in their pathogenesis. Co-drugs that linked two active molecules into a single compound and released the drugs after administration, which offering improved efficacy and tolerability than individual drug mixtures or monotherapy. In this work, five new co-drugs ODV-NSAIDs were synthesized from O -desmethylvenlafaxine (ODV) with non-steroidal anti-inflammatory drugs (NSAIDs) to achieve synergistic antidepression and anxiolytic effects. In vitro stability studies exhibited that these co-drugs can be metabolized into two single drugs within 60 min in simulated intestinal fluid. In both acute and chronic LPS-induced models, co-drug ODV-NAP significantly ameliorated depressive-like behaviors, evidenced by increased sucrose preference, reduced immobility in the tail suspension test (TST) and forced swim test (FST), and enhanced locomotion in the open field test (OFT). Furthermore, ODV-NAP decreased brain levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) and malondialdehyde (MDA), while elevating serotonin (5-HT), norepinephrine (NE), and superoxide dismutase (SOD) activity. Nissl staining confirmed ODV-NAP significantly attenuated hippocampal neuronal damage. Moreover, western blotting revealed ODV-NAP inhibited the TLR4/NF-κB signaling pathway and upregulated BDNF and p-TrkB protein expression. ODV-NAP also inhibited LPS-induced p65 nuclear translocation in BV-2 microglia in vitro , and caused no toxicity in histology. Thus, co-drug ODV-NAP represented a promising novel candidate for treating depression and anxiety.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2026
·
IF 9.2
Liru Ma,Zhen Feng,Chao Zhang,Haode Chang,Wenjing Zhang,Quanmiao Zhang,Yihong Bao,Chunhe Gu
This study evaluated polyphenol fractions from unripe (J1), underripe (J2), and ripe (J3) jackfruit to explore stage-dependent industrial potential. Free (FP), conjugated (CP), and bound phenolics (BP) were isolated and assessed for antioxidant and enzyme inhibitory activities. Ripening significantly altered composition and bioactivity. CP antioxidant capacity and inhibition of α-glucosidase, pancreatic lipase, and xanthine oxidase increased progressively from J1 to J3, while FP and BP showed fluctuating trends. Ripe fruit (J3) exhibited the strongest CP-related bioactivities and a more diverse conjugated phenolic profile, whereas early stages had greater FP compositional complexity. Molecular docking supported these findings, identifying cyclomorusin as a potent ligand for α-glucosidase (−9.6 kcal/mol) and xanthine oxidase (−11.8 kcal/mol), and cyclokievitone for pancreatic lipase (−6.7 kcal/mol). These results demonstrate stage-specific functional differentiation and support targeted industrial utilization for health promotion.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Yue-Yang Zhang,Zi-Xin Chen,Gang Nie,Huiling Wang,Qiaolin Li,Shi-Yu Liu
Bio-activated esters containing a functional alcohol and an acid moiety as recognition unit have emerged in bioimaging and drug discovery. When the alcohol moiety is designated as an imaging dye or a drug, disease-related enzyme activity can be visualized, or the drug can be delivered to disease lesions with aberrant enzyme expression. However, although the carboxylic acid moiety is co-delivered to enzyme-overexpressing sites along with the alcohol part, the leaving acid groups are typically non-functional. Therefore, we sought to develop a dual-functional ester design platform by incorporating a biologically active acid with functional alcohol moieties. In this study, we selected carboxylesterase 2 (CES2) as the target enzyme and first proposed aspirin-based esters as a potential CES2-targeting scaffold. A prototype compound ( HFC-AS ) was initially constructed and characterized as a dual-functional ester, which exhibited high sensitivity and specificity for CES2 sensing and significantly improved antitumor efficacy compared to the combined addition of HFC-OH and aspirin. Subsequently, an aspirin-based fluorescent probe ( DDX-AS ) was developed, displaying an ultrafast reaction rate toward CES2 while maintaining high sensitivity and selectivity. Consequently, CES2 activity in living systems could be rapidly detected with high specificity, enabling imaging of both subcutaneous transplanted and pulmonary metastatic melanoma. Furthermore, an aspirin-based prodrug ( SN-38-AS ) was developed and demonstrated a higher tumor growth inhibition rate (98%) than the clinically used prodrug irinotecan (89%), while also exhibiting an improved safety profile. Collectively, a new strategy for CES2-targeted bioimaging and drug delivery was proposed and evaluated, showing great potential for disease diagnosis and drug development.
✓ 已验证
产品关联
DOI
高影响因子
Current Research in Food Science
·
2026
·
IF 7.7
Donglei Zheng,Mengle Li,Ying Qian,Xiangsong Chen,Jianglin Chen,Long Jin,Lili Cao,Min Pang,Jiaying Huo
Sialic acid (Neu5Ac) possesses a wide range of bioactivities; however, its instability under acidic conditions prevents it from effectively reaching the intestine. This underscores the urgent need to develop a stable delivery system capable of protecting Neu5Ac from gastric degradation and achieving its controlled release in the intestine. To enhance the stability and achieve controlled release, Neu5Ac was encapsulated within water-in-oil-in-water (W1/O/W2) emulsion-based microcapsules in this work. S2 emulsions featured a “dual-chamber, tri-phase” structure, comprising a Neu5Ac-chitosan fluid gel (inner aqueous phase), maize oil (oil phase), and an external aqueous phase of 13% sodium starch octenyl succinate, 5% β-cyclodextrin, 1.5% sodium caseinate, and 0.5% carboxymethyl cellulose. Microstructural analyses confirmed spherical morphology with core-shell architecture and bimodal droplet distribution (1-10 μm). In addition, S2 emulsions exhibited an encapsulation efficiency (EE) of 92.44%, a zeta potential of −60.80 mV, a Sauter mean diameter (d 3 , 2 ) of 1.337 μm, shear-thinning behavior and weak gel network stability. S2 emulsions were spray-dried to obtain S2 microcapsules. S2 microcapsules exhibited an EE of 89.43% and a d 3 , 2 of 3.839 μm, while FT-IR and XRD verified physical encapsulation without covalent bonding. In vitro digestion, S2 microcapsules exhibited controlled release behavior during simulated intestinal digestion, with a Neu5Ac release rate of 85.61% after 120 min. In mice, microencapsulated Neu5Ac increased the relative abundance of Akkermansia , Lactobacillus , and Bifidobacterium . This system demonstrates promising potential for the co-delivery and controlled release of Neu5Ac, and shows great promise for expanding its applications in the food industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Innovative Food Science & Emerging Technologies
·
2026
·
IF 6.9
Liyuan Ma,Danyang Liang,Xiangrui Ren,Li Niu,Wenqing Zhao,Wenhao Li
To address the quality limitations of gluten-free potato noodles caused by the lack of a gluten network, this study elucidates the influence of sodium alginate (SA, 0, 0.5, and 1.5%) and zein (0, 10, 15, and 20%), individually and in combination, on the thermomechanical properties of potato dough and the quality of twin-screw extruded noodles. Mixolab analysis showed that SA significantly increased water absorption, while zein delayed protein weakening ( P < 0.05). Their combination enhanced dough's thermomechanical stability and starch gelatinization. At 0.5% SA and 10% zein, dough development time was minimized (0.70 min), and retrogradation resistance was optimal (C5–C4 = 0.63 Nm). Cooking loss was significantly reduced (16.41–30.71%) with SA at ≤1.5% and zein at ≤15%. Low-field nuclear magnetic resonance revealed their synergistic improvement of internal water distribution in noodles. Textural analysis indicated significantly reduced hardness, chewiness, and resilience for all SA-zein combinations except 1.5% SA with 20% zein, with maximum reductions (60.40%, 68.11%, and 38.18%) at 0.5% SA and 20% zein. Microscopy and quantitative network analysis confirmed that a moderate SA (<1.5%) promoted a continuous zein-starch network, whereas excess SA led to aggregation and structural deterioration. Color and in vitro digestion analyses revealed that SA and zein together enhanced redness and increased the content of rapidly digestible starch. In summary, at appropriate concentrations, the combination of SA and zein can synergistically improve noodle quality, thereby providing both theoretical and practical guidance for the rational design of gluten-free extruded noodles.
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2026
·Vol. 19
·期 10
·302
·
IF 3
Yu Haichao,Huang Jiayue,Zhao Jinfeng,Luo Wang,Li Jianjun,Liu Xiaojun
With the large-scale development of the food industry, hydrogen peroxide (H₂O₂), a potent oxidizing agent, has been illicitly used by some manufacturers for bleaching, decolorization, and preservation, making it a significant potential risk in the field of food safety. Therefore, elucidating the hazardous mechanisms of H₂O₂ residues in food is of paramount importance for strengthening food safety monitoring systems and safeguarding public health. In this study, a label-free and highly sensitive sensing platform based on 4-cyano-4'-pentylbiphenyl (5CB) liquid crystal (LC) microdroplets was developed for the detection of H₂O₂ and catalase (CAT). The sensor enables the detection of H₂O₂ and CAT by monitoring the distinct optical pattern transitions of dodecanal-doped 5CB liquid crystal microdroplets under various conditions using polarized optical microscopy (POM). The results demonstrate that under pH 11 conditions, the limit of detection (LOD) for H₂O₂ is 0.01% (w/v), and for CAT is 0.01 ng/mL. Fourier Transform Infrared Spectroscopy (FTIR) was employed to characterize the post-reaction system, verifying the chemical transformations. Furthermore, simulated testing on food samples was successfully conducted, suggesting that this sensor holds significant potential for applications in food safety, biomedicine, and analytical detection. However, the optical sensing platform is sensitive to changes in ambient humidity and temperature, which may limit its direct application in complex food matrices. Although the system enables intuitive interpretation of POM textures, future work should investigate matrix interference, quantitative image analysis, portable readout integration, and validation using authentic food samples.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）